Decorin( DCN) is one of the leucine-rich proteoglycan family of small molecules.It is recog-nized as a tumor suppressor gene.DCN can inhibit tumor cell proliferation and metastasis by binding and inactiva-tion of transforming growth factor beta( TGF-β) and inhibiting the expression of vascular endothelial growth fac-tor or by activating the signaling pathway that promotes cell proliferation by EGFR/MAPK/p21.it plays an impor-tant effect on tumor development,angiogenesis and metastasis.DCN is expressed in various malignant tumors,By up or down regulation,DCN can exert its anti tumor activity,as well as reducing or delaying the occurrence and development of many kinds of malignant tumor.DCN gene may be a potential target for the treatment of many kinds of malignant tumors.

OBJECTIVE:To investigate the characteristics and rules of leukocytopenia induced by essential antipsychotic medi-cines in psychotic in our hospital,and provide reference for the clinical treatment. METHODS:1 345 psychotics received essential antipsychotic medicines from Nov. 2012 to Nov. 2014 in our hospital were collected,and retrospective analysis was adopted to sta-tistically analyze the age,sex and the drug type,dose and treatment time that caused leukocytopenia,symptom duration,white blood cell(WBC)count,clinical manifestation and prognosis. RESULTS:In the 1 345 patients,there were 205 patients with WBC sustained reduction in the process of medication,accounting for 15.24%. The incidence rate of leukocytopenia in patients older than 60 years old reached up to 54.15%;57 patients with leukocytopenia was induced by combination medication,accounting for 20.14% in the total cases of combination medication,and it was significantly higher than the incidence rate of single medication (13.93%),the difference was statistically significant(P<0.05);incidence rate of leukocytopenia induced by the first generation of antipsychotic medicines was 63.36%,which was significantly higher than the second generation(36.64%),the difference was sta-tistically significant(P<0.05);there were 113 patients with the WBC count of 3.0×109-3.9×109 L-1,accounting for 54.63%,com-pared with other WBC count groups,there were significant differences(P<0.05);most of the patients had no discomfort and obvi-ous clinical symptoms,and all patients were improved or recovered after symptomatic treatment. CONCLUSIONS:The incidence rate of antipsychotic in elderly patients with essential antipsychotic medicines is relatively high,and the incidence of antipsychotic is more likely to be caused by combination medication and the first generation antipsychotic medicines. Although leukocytopenia can be cured,it may increase the risk of infection,so blood routine should be regularly checked and paid great attention during treatment.

Education of the adolescent includes sexual physiological education,sexual psychological education and sexual moral education.of both the sexual psychological education and sexual moral deucation,which are often ignored,are the very important two aspects.

OBJECTIVE:To establish an HPLC method for determination of Emodin in Yanyan granules.METHODS:The stationary phase was Diamonsil C18(150mm? 4.6mm,5? m)column and the mobile phase was methanol-0.1% phosphoric acid(68∶ 32),with detection wavelength set at 254nm.RESULTS:Within the range of 0.26～ 3.90? g Emodin had a good linearity,and the average recovery was 96.73%(RSD=1.49%).CONCLUSION:The method is accurate,sensitive,reproducible,simple and can be used for the quality control of Yanyan granules.

Cytomegalovirus (CMV),a DNA herpusvirusesw,is the most common virus leading to intrauterine infetcion.It is transmitted from mother to fetus through placenta,and the neonates who suffering from congenital symptomatic infections may have sensorineural hearing loss,hepatosplenomegaly,microcephaly,chorioretinitis etc.Studies have suggested that the characters of infections on pregnant women,such as the time of infection,the serological results and virus load in amniotic fluid are associated with the outcome of offsprings.This paper is to review the risk of CMV infections on women at childbearing age,screening of CMV infections on pregnant women and the prenatal diagonosis of fetal CMV infection.

In recent studies,it has been demonstrated that the clinical efficacy of surfactant is poor for late protein infants.Besides,this kind of infants have not infection or other risk factors that may lead to respiratory distress syndrome(RDS).Thus,it is speculated that the pathogenesis of RDS of late preterm infants is different from preterm infants.New researches show that,as the key point of Lung fluid transport via Epithelium,α-ENaC plays an important role in the pathogenesis of RDS of late preterm infants through the inhibition of lung fluid clearance and activity of surfactant.This review highlights the most important mechanisms of Lung fluid absorption barrier in RDS of late preterm infants.

The research on the clinical characteristic and epidemiology in Henoch-Schonlein purpara of children indicates that genetic factors are closely connected with the disease and pathological process.In recent years,molecular biology studies show that C1 GALT1 gene,IL gene,vasomotor and endothelial function regulation genes,angiotensin-converting enzyme gene,angiotensinogen gene,MEFV gene and so on,which have aberrant IgAl giycosylation,are closely related with pathogenesis of Henoch-Schonlein purpura in children.The paper reviews the progress of genetic mechanism associated with Henoch-Schonlein purpura in recent years.

Vitamin D,an immune regulator,plays an important role in innate immune function of antibacterial peptide.Vitamin D takes part in the innate immune function via toll-like receptor in response to pathogen,then release antibacterial peptide.This study describes the antibacterial peptide constitution and the functions,effection of activeted Vitamin D in innate immune function of antibacterial peptide.

Minimal residual disease(MRD)iS the origin of relapse in leukemia.So it's significant to quantitate MRD in clinical.At present, there are three MRD quantitative assays, such as flow cytometric detection of abnormal immuno-phenotype,polymerase chain reaction(PCR)amplification of antigenreceptor genes and fusion transcripts.Because every measure has its own principle and feature.the clinical applications are different.Selecting suitable assays based on the characteristic of patients can help to quantitate MRD exactly.

Objective To investigate the clinical significance of detection of serum IL -6,IL -17, GM-CSF and TNF -αlevels in patients with rheumatoid arthritis.Methods A retrospective research was made in 79 cases with rheumatoid arthritis,and they were divided into stable period and active period,and another 40 healthy persons were chosen as control group.IL -6,IL -17,GM-CSF and TNF -αlevels were compared,and the relevance was investigated.Results CRP,ESR and DAS28 of active period [39.14 (35.81 )mg/L,41.92 (33.27)mg/L, 4.85(2.74)]were higher than those of the stable period[21.07(16.53)mg/L,29.57(25.14)mg/L,2.88(1.76)] and control group[5.92(2.80)mg/L,3.19(2.24)mg/L],the differences were statistically significant (F =7.013, 6.224,2.136,all P <0.05).Serum levels of GM-CSF,IL -6 and TNF -αof active period[33.81(26.97)pg/mL, 47.23(42.81 )pg/mL,75.35 (41.69 )pg/mL]were higher than those of control group [6.19 (5.11 )pg/mL, 4.68(5.21)pg/mL,33.18 (20.94)pg/mL]and stable period [12.81 (9.76 )pg/mL,8.46 (10.37 )pg/mL, 43.05(38.14)pg/mL],the differences were statistically significant (F =5.328,6.117,8.016,all P <0.05).And there was no significant difference in IL -17 level between the groups (F =0.776,P >0.05).No correlation was observed between IL17 and DAS28,GM-CSF and IL -6 (r =0.213,0.167,0.316,all P >0.05),TNF -αand DAS28,GM-CSF,IL -6 and IL -17 (r =0.761,0.729,0.549,0.421,all P <0.05 ),IL -6 and DAS28 and GM-CSF (r =0.648,0.626,all P <0.05),GM-CSF and DAS28 had good correlation (r =0.563,P <0.05). Conclusion IL -6,GM-CSF and TNF -αlevels were positively correlated with the progression of rheumatoid arthritis,joint detection is of great value in monitoring the disease.