Breast Neoplasms ; metabolism ; pathology ; Female ; Humans ; Immunohistochemistry ; standards ; In Situ Hybridization ; standards ; Neoplasm Invasiveness ; Receptor, Epidermal Growth Factor ; analysis ; metabolism

Objective To observe the clinical efficacy and security of anti-tuberculosis combined with highdose methylprednisolone in treatment of advanced tuberculous meningitis.Methods 77 patients with advanced tuberculous meningitis in our department were randomly divided into the observation group(39 cases)and the control group (38 cases).All of the patients were given initiate antiphthisie treatment,the control group were added dexamethasone,20mg/d,iv drip,then was maintained after improved;the observation group were added high-dose methyprednisolone(500mg/d)by intravenous injection for five days,then was given methyprednisolone 80mg/d to maintain.Two groups were treated with hormone no more than three months.Then the clinical efficacy and adveme reaction were compared.Results The observation group:32 cases of healing,6 cases of effective,1 case of inefficient,the total effective rate was 97.4%and the control group were 22,8,8,78.9%;the difference of total effective rates in the two groups was statistically significant(x2=7.57,P＜0.05).The time of consciousness restoration and defevesence,the white blood cell count,protein chloride and glucose content of cerebrospinal fluid of the observation group were(2.7±1.3)d,(13±5)d,(112.37±27.57)×106/L,(3.04±0.82)g/L,(32.7±6.4)mmol/L and(1.67±0.65)mmol/L;and the control group were(5.2±2.4)d,(19±6)d,(93.74±20.18)×106/L,(1.92±0.64)g/L,(25.4 ±5.2)mmol/L and(1.04±0.34)mmol/L,the difference was statistically significant(t=5.70,4.77,3.88,6.67,5.48,5.31,P＜0.01);The difference of adverse reaction rate between the two groups was not statistically significant (P＜0.05).Conclusion The clinical efficacy of anti-tuberculosis combined with high-dose methylprednisolone in treatment of advanced tuberculous meningitis was better and had advantages in improving symptoms and signs without enhancing the rate of adverse reaction and it was worth to promote in clinical.

OBJECTIVE: To analyze the situation and the related problems of Chinese patent drugs including toxicity components used in pediatrics, and to provide the suggestion for the development of Chinese patent drugs for pediatrics. METHODS: 264 Chinese patent drugs which were used in pediatrics were collected from national drug standards, and compared with toxicity drug list. The utilization of toxicity drugs were analyzed statistically. RESULTS: About half of Chinese patent drugs for pediatric included toxicity components. Major problems are complex resource of toxicity components, overdose of toxicity components, single disease and pattern, unscientific determination of amount, etc. CONCLUSION: The in-depth study of Chinese patent drugs requires perfecting national drug standards, to developing more rigorous special approval requirements, to establishing the re-evaluation system and to eliminating unsuitable category of Chinese patent drugs.

AIM: To estimate the efficacy of milrnone in treatment of congestive heart failure. METHODS: 122 cases of chronic congestive heart failure with different etiology were divided into milrinone group and dabutamine group randomly. Milrinone group accepted milrinone (10 mg?d -1 ) for 5 days, and dabutamine group accepted dabutamine (160 mg?d -1 ) for 5 days. The efficacy of treatments was evaluated. RESULTS: The effectiveness rates of cardiac function improvement were 93.9 % and 76.7 % in milrinone group and dabutamine group, respectively (P

Objective To describe the status of resilience and self-care behaviors in chronic heart failure patients and to explore the relationship between resilience and self-care behaviors. Methods It was a cross-sectional survey. The Connor-Davidson Resilience Scale, the self-care behaviors with heart failure questionnaire and the demographic scale were delivered to 150 chronic heart failure patients in Shanghai Changhai Hospital. Results The score of self-care behaviors was (2.63 ± 2.01) points for chronic heart failure patients. The resilience score was (57.56 ± 8.51) points. The resilience was significantly related to the self-care behaviors(r=0.427, P<0.01), and which explained 32.4%of the total variation. Conclusions The chronic heart failure patients have poor self-care behaviors and resilience. More attention should be paid and more action be taken to improve the resilience, then improve the self-care behaviors of patients.

Triple-negative breast cancer (TNBC) is a subtype of breast cancer known for the poor prognosis due to its strong invasiveness, high recurrence rate, and lack of effective treatment. Therefore, there is an urgent need to find targeted therapy for TNBC. Cathepsin A (CTSA) is an acidic serine carboxypeptidase that is highly expressed in various tumor tissues. However, the role and molecular mechanism of CTSA in TNBC are still unclear. This study found that the expression of CTSA was upregulated, and the high expression of CTSA was positively correlated with the poor prognosis of TNBC. The results further showed that knocking down CTSA inhibited the proliferation, invasion, and colony formation of TNBC cells, improved drug sensitivity of cells, and inhibited the progression of TNBC. Mechanistically, CTSA inhibited the ubiquitination and degradation of the promyelocytic leukemia protein (PML) protein by blocking the interaction between PML and its E3 ubiquitin ligase RNF4, thus maintaining the stability of PML nuclear bodies (PML-NBs). The inhibitor of CTSA had a positive therapeutic effect on inhibiting the characteristics of TNBC stem cells. In conclusion, this study demonstrates that inhibiting CTSA to decrease the stability of PML protein may be a promising therapeutic strategy for TNBC. All animal experiments in this experiment were approved by the Ethics Committee of Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College (approval number: IMB-20240326D502).

Geriatric patients may present with atypical symptoms for COVID-19. This case aims to highlight how an elderly patient with two to three weeks of low-grade fever of unknown origin was managed. The family medicine resident was able to assess the patient holistically and admit her to the right discipline with the proper contact precaution.

Objective: To investigate the serum bilirubin level in patients with acute cerebral infarction and its relationship with the prognosis. Methods: A total of 177 patients with acute cerebral infarction (onset ≤2 weeks) were met the diagnostic criteria, 38 patients whose basic information were matched but without acute cerebral infarction (onset >2 weeks), and 102 controls were selected. The serum bilirubin level and direct bilirubin level were detected at 72 hours after admission in all groups, the indirect bilirubin value was calculated, and the biochemical indexes, such as blood lipids, blood glucose, high sensitive C-reactive protein and fibrinogen (FIB) were detected. Neurological deficit in the acute cerebral infarction group was assessed on the day of admission using the National Institutes of Health Stroke Scale (NIHSS). The prognostic evaluation was conducted using the modified Rankin scale (mRs) at 3 months after the onset of stroke. Results: Circled digit oneThe serum bilirubin and direct bilirubin levels in the acute cerebral infarction group were significantly higher than those in the non-acute cerebral infarction group (P < 0.05) and control group or (P < 0.01), and the indirect bilirubin level only higher than that of the non-acute cerebral infarction group (P < 0.05). The mean level of the three kinds of serum bilirubin in the non-acute cerebral infarction group was lower than that in the control group; however, there was no significant difference. Circled digit twoWith the increased severity of neurological deficit in the acute cerebral infarction group, the mean levels of the 3 kinds of bilirubin tended to increase, however, there was no significant difference (P < 0.05). Circled digit threeThree months after the onset, both the serum total bilirubin and direct bilirubin levels at admission in patients with good prognosis (mRs, 0 to 2) in the acute cerebral infarction group were higher than those with poor prognosis (mRs, 3 to 5). The differences were statistically significant (P > 0.05). Circled digit fourMultivariate logistic regression analysis showed that the increased serum total bilirubin level was independently associated with the good prognosis (OR, 0.807, 95% CI: 0.718-0.907. P < 0.01). Conclusion: The serum bilirubin levels showed stressful increase in patients with acute cerebral infarction. The increased serum bilirubin level is not significantly associated with the severity of neurological deficit, but it is associated with good prognosis. The increased serum bilirubin level may be a protective defense response of the body for stroke events.

Cisplatin,carpolatin and oxaliplatin are platinum-based drugs that are widely used in cancer chemotherapy. Peripheral neuropathy is major dose-limiting side effect of platinum-based drug. Accordingly, this side effect restrict its clinic application. This review will focus on the research advancement on the mechanism of dorsal root ganglia neurons apoptosis in cellular and molecular biology.

Objective To explore the factors related with osteoporosis in middle-aged men who accepted high education. Methods 306 men were investigated about their bone density, also their behaviors, diet, exercise and so on. Results The incidence of osteoporosis increased with the aging. The Logistic regression showed that smoking (OR=4.61) and alcohol intake (OR=1.11) were the risk factors for osteoporosis,while the milk intake (OR=0.19) and exercise (OR=0.13) were the protective factors. Conclusion The low bone mass and osteoporosis of middle-aged men accepted high education are associated with the age, smoking, alcohol or milk intake and exercise.