In the article the design and making method of improved chincap with Lengthwise hook and protraction appliances are introduced.The appliances are mainly used to treat early-stage skeletal crossbite of antCrior teeth,which can't be solved by simple intramaxillary anchorage appliances.The analysis of pre and post cephalometrics of 11 cases yields signifi- cant differences (P

Neurodegenerative diseases are chronic lesions caused by neuronal degeneration and secondary demyelination.Recent studies have shown that a DNA/RNA binding protein named fused in sarcoma/translocated in liposarcoma protein (FUS/TLS) is associated with neurodegenerative diseases,such as amyotrophic lateral sclerosis (ALS),frontotemporal dementia (FTD) and polyglutamine diseases.Mutations or dysfunction in FUS/TLS are responsible for the abnormal processes in the related gene transcription,RNA processing,transportation and translation and are highly correlated with the development of neurodegenerative disease.Discovery and the research of FUS/TLS not only contribute to the understanding of neurodegenerative disease at RNA level,but also provide a new way for the prevention and treatment of neurodegenerative diseases.

This article made primary reseach on the following aspects: fetus's legal status and civil rights,applied law of compensation for damages,legal responsibility' assumed,etc.

Objective To investigate the expression of microRNA in plasma of gallbladder cancer and its clinical significance.Methods miRNA microarray was performed in seven gallbladder cancer tissues and seven adjacent normal gallbladder tissues to identify differentially expressed miRNA.The results were validated in plasmas of 40 gallbladder cancers and 20 health people with real-time polymerase chain reaction (RT-PCR).Results Eleven gallbladder cancer-related miRNAs were identified with miRNA microarray analysis.Among them,miRNA-21,miRNA-370,miRNA-187,miRNA-122,and miRNA-202 were up-regulated,and let-7a,miRNA-200b,miRNA-143,miRNA-31,miRNA-335,and miRNA-551 were down-regulated in gallbladder cancers.Moreover,let-7a,miRNA-21,miRNA-187,miRNA-143,miRNA-202,and miRNA-335 were validated with RT-PCR,which were consistent with miRNA microarray results with significant difference between gallbladder cancers and health people (P ＜ 0.05).Furthermore,miRNA-187,miRNA-143,and miRNA-202 were related to clinical and pathological features(P ＜0.05).Conclusions These data indicate that The differentially expressed plasma miRNAs are related to gallbladder carcinogenesis and might be the novel biomarkers of gallbladder cancers.

Objective To discuss value of transvaginal ultrasound combined with abdominal ultrasound in the diagnosis of gynecologic acute abdomen.Methods 102 cases with gynecologic acute abdomen who were treated in our department were selected,they got through transvaginal ultrasound combined with abdominal ultrasound.The diagnosis result and pathologic diagnosis,simple ultrasound diagnosis result were compared.Results Compared with transvaginal ultrasound,abdominal ultrasound diagnosis coincidence rate obviously decreased,the difference was statistically significant (t =5.17,P <0.05 ),ultrasound combination diagnosis coincidence rate all obviously increased,the difference was statistically significant (t =11.25,P <0.01 ).The highest ultrasound combination diagnosis coincidence rate was heterotopic pregnancy (100.00%),the lowest was perforation uterus (75.00%). Average ultrasound combination diagnosis coincidence rate in this research was 86.78%,misdiagnosis rate was 1.96%,and missed diagnosis rate was 3.92%.Conclusion Accuracy rate of transvaginal ultrasound combined with abdominal ultrasound pathologic diagnosis results,have these following advantages,such as high accuracy rate,rapid diagnosis,suitable for gynecologic acute abdomen diagnosis.

Synergistic Innovation is the developing trend and requirements of the times and society.Southern Medical University practiced joint mode among colleges and universities for excellent medical talent cultivation.Based on preponderant discipline and taking mechanism innovation as the key points,Southern Medical University actively explored the diversification mode of synergistic innovation.

To investigate the relationship between the expression of cyclooxygenase-2 (COX-2) and menstrual cycle, the regulatory effects of 17-beta-estradiol (E(2)) and medroxyprogesterone acetate (MPA) on the expression of COX-2 in cervical cancer Hela cells were examined. Cervical cancer specimens were obtained from 47 pre-menopausal patients. The phase of menstrual cycle was determined by case history and HE staining of uterine endometrium. COX-2 was immunohistochemically stained by SABC staining and the staining intensity was determined with computerized image analysis system. Hela cells were incubated with alcohol, E(2), E(2)+MPA, MPA for 12, 24 and 48 h respectively. The expression of COX-2 in Hela cells was detected by Western blotting and reverse transcriptase-polymerase chain reaction (RT-PCR). Our results showed that the expression of COX-2 was significantly higher during proliferative phase than secretory phase (P<0.05), but there was no difference in the positive rate between proliferative phase and secretory phase (P>0.05). Incubation with E(2) could significantly enhance the expression of COX-2 continually. On the contrary, E(2)+MPA and MPA alone could decrease the expression of COX-2 as compared with the control and E(2) group (P<0.05 and P<0.01 respectively). It is concluded that the expression of COX-2 in cervical cancer of pre-menopausal patients and Hela cells was regulated by estrogen/progestogen.
Cyclooxygenase 2/genetics ; Cyclooxygenase 2/*metabolism ; Estradiol/*pharmacology ; Hela Cells ; Medroxyprogesterone Acetate/*pharmacology ; Uterine Cervical Neoplasms/*enzymology

Acquired Hyperostosis Syndrome ; therapy ; Combined Modality Therapy ; Humans ; Integrative Medicine ; Male ; Middle Aged

Objective To study the length of hypertension history and its related factors in patients with both ischemic cerebral vascular disease(ICVD) and hypertension.Methods The profile of hypertension history in ICVD patients from our hospital between 1990 and 1999 was summarized.Hypertension history meant the time interval between onset of first symptomatic ICVD and establishment of diagnosis of hypertension.The related factors' influence on the length of hypertension history was analyzed by means of multiple linear regression model.Results In ICVD patients with hypertension,the mean length of hypertension history was 14.29?10.63 years,the median was 11 years.In multiple linear regression model,increased age when hypertension was diagnosed,diabetes mellitus,smoking,peasant(army man as control group) were independent factors that shortened hypertension history.Antihypertensive treatment independently lengthened hypertension history.Conclusion The length of hypertension history varies greatly,50% of patients developed the first ICVD in 11 years after the diagnosis of hypertension was established .Coexisting smoking and diabetes mellitus hasten while control of arterial blood pressure postpone onset of ICVD in patients with hypertension.

Objective To study the effective methods for extraction of clenbuterol from biological material. Methods Clenbuterol in blood samples was extracted with 5 different methods,the extracts from various extraction methods were determined by gas chromatography-mass spectrometry(GC-MS?雪. The efficiency of 5 extraction methods was compared with each other. Results The standard curve showed good linear relationship with a range from 0.01-10.0 mg/L clenbuterol hydrochloride(r=0.999 9?雪. The detection limit was 0.005 mg/L. After adding 1.0 ?g/ml clenbuterol hydrochloride into blood sample,the blood sample was immersed and extracted in 0.01 mol/L HCl and absolute alcohol.The extracts were extracted by chloroform at pH value of 11. The average recovery and RSD of this method were 96.5% and 5.21% respectively(n=6?雪. Conclusion The method showed easy operation and reliable results ,it was suitable for determination of clenbuterol in biological material without deviation.