Objective To investigate the expression of C-C chemokine ligand 5(CCL5) in head and neck squamous cell carcinoma(HNSCC),and explore the effect of CCL5 on the biological characteristics of laryngeal carcinoma cells.Methods Gene Expression Profiling Interactive Analysis(GEPIA) database was used to investigate the expression of CCL5 in HNSCC.The laryngeal carcinoma cells TU177 were transfected with siRNA(siRNA group),and the control(NC) group was set up.The cell proliferation,migration,cycle and apoptosis of each group were detected by CCK8 assay,cell scratch test and flow cytometry respectively.RT-PCR and Western blot were used to detect the knock-down efficiency of CCL5 and the mRNA transcription and protein expression of multidrug resistance protein 2(MRP2) and bcl-2-associated x protein(Bax).Results The expression of CCL5 in HNSCC was higher than that in normal tissues(P <0.05).Compared with NC group,siRNA showed higher knock-down efficiency(t=12.898 and 22.656 respectively,each P <0.01);siRNA interference with CCL5 inhibited the proliferation and migration of laryngeal carcinoma cells,and promoted the late apoptosis of laryngeal carcinoma cells and the expression of apoptosis protein Bax(t=2.600～11.667,each P <0.05).Conclusion CCL5 was highly expressed in HNSCC,while siRNA interference with CCL5 inhibited the proliferation,migration and promoted apoptosis of laryngeal carcinoma cells TU177 by up-regulating the expression of Bax,which laid a foundation of the possibility of CCL5 as a new target for the treatment of laryngeal carcinoma.

Humans ; International Classification of Diseases ; International Cooperation ; Occupational Diseases ; classification

Objective To investigate correlation between vitamin D and glucose metabolism,lipid metabolism, insulin resistance,early secretory function of pancreatic islets in postmenopausal women with type 2 diabetes. Methods 115 postmenopausal women with type 2 diabetes were selected as observation group.115 postmenopausal women without type 2 diabetes were selected in hospital at same period as control group.All patients received detection of vitamin D,glucose metabolism,lipid metabolism,insulin resistance,early secretory function of pancreatic islets. Correlation between vitamin D and glucose metabolism,lipid metabolism,insulin resistance,early secretory function of pancreatic islets were analyzed.Results Fasting blood glucose,postprandial 2h blood glucose,glycosylated hemoglobin, body mass index,total cholesterol,triglyceride,low density lipoprotein cholesterol,insulin resistance index in the observation group were higher than the control group [(8.5 ±1.2)mmol/L vs (5.1 ±0.6)mmol/L,(13.4 ± 1.5)mmol/L vs (7.8 ±0.7)mmol/L,(8.7 ±1.1)% vs (5.6 ±0.5)%,(25.7 ±1.0)kg/m2 vs (22.3 ±0.4)kg/m2 , (5.6 ±0.7)mmol/L vs (4.5 ±0.4)mmol/L,(2.3 ±0.5)mmol/L vs (1.7 ±0.3)mmol/L,(3.5 ±0.6)mmol/L vs (2.2 ±0.4)mmol/L,(3.6 ±0.5)vs (2.5 ±0.4),t =9.667,10.179,8.535,4.524,5.443,6.529,8.909,7.406, all P <0.05].High density lipoprotein cholesterol,insulin first secretion phase peak factor,pancreatic islet βcell function index,fasting insulin,vitamin D in the observation group were lower than the control group [(1.0 ± 0.2)mmol/L vs (1.2 ±0.3)mmol/L,(5.2 ±1.3)vs (8.4 ±1.6),(135.8 ±11.7)vs (194.3 ±19.2),(4.1 ± 0.9)IU /mL vs (10.8 ±0.6)IU /mL,(10.8 ±2.1)ng/L vs (27.4 ±3.8)ng/L,t =5.087,9.153,7.370,10.414, 18.371,all P <0.05].Vitamin D was significantly negatively correlated with fasting blood glucose,postprandial 2h blood glucose,glycosylated hemoglobin,body mass index,total cholesterol,triglyceride,low density lipoprotein choles-terol,insulin resistance index(r =-0.537,-0.512,-0.469,-0.387,-0.325,-0.316,-0.372,-0.457,all P <0.05).Vitamin D were significantly positively correlated with high density lipoprotein cholesterol,insulin first secretion phase peak factor,pancreatic islet βcell function index,fasting insulin(r =0.351,0.418,0.674,0.316,all P <0.05).Conclusion Vitamin D deficiency in postmenopausal women with type 2 diabetes may be involved in glucose -lipid metabolism and insulin resistance,but also may be one of the reasons leading to decreasing of early secretory function of pancreatic βcells.

Objective To investigate the antimicrobial resistance of Enterococcus f aecalis and Enterococcus f aecium isolated from the First Affiliated Hospital of Chongqing Medical University between 2009 and 2012 .Methods Antimicrobial susceptibility testing was carried out according to the unified protocol .The dates were analyzed by WHONET 5 .6 software according to clinical and laboratory standards institute(CLSI) of 2012 .Results A total of 783 non-repetitive Enterococcus f aecalis and 664 non-repeti-tive Enterococcus f aecium isolates were collected .The strains were still highly susceptible to linezolid and vancomycin .The resist-ance rates were all less than 2 .0% .The resistance rates of vancomycin to Enterococcus f aecalis and Enterococcus f aecium were 0 .1% and 1 .4% ,respectively .The percentage of Enterococcus f aecalis resistant to ampicillin ,penicilin and nitrofurantoin were 5 .7% ,2 .6% and 2 .2% ,respectively .About 32 .9% of Enterococcus f aecalis isolates were resistant to gentamicin .The resistance rates of ampicillin and penicillin to Enterococcus f aecium were more than 90 .0% .Conclusion Enterococcus f aecalis is main En-terococcus in the First Affiliated Hospital of Chongqing Medical University .There is an obvious difference between the antibiotic re-sistance of the Enterococcus f aecalis and Enterococcus f aecium .So ,monitoring drug resistance of the Enterococcus shows great sig-nificance to the clinical treatment .

Objectlive To explore the health effects of passive smoking on children. Methods During the period of March 1,2000-Feb. 28, 2001, the frequencies of respiratory diseases (eg. cold,chathitis,pneumonia and asthma)were investigated among 1031 4-6-year old children in 4 day-care nursing schools in Lanzhou, 485 children of which were selected for the determinations of the concentration of sulphocyanate,bacteriolytic enzyme and immunoglobulin (IgG, IgA, lgM)in saliva. Results The proportion of the numbers of children suffering from respiratory diseases and cold at least 4 times per year to all of 1031 children increased with the increase of intensity of passive smoking exposure (respiratory disease: X2=13,8, P

In vivo,dendritic cells(DCs)are the most powerful antigen-presenting cells (APCs).DCs based neoplasm immunotherapy has recently become the focus of research.DCs can be used not only as separate cell vaccine to mediate anti-tumor immunity,but can also be combined with cytokines,immune cells,and other methods in tumor immunotherapy. In animal models and clinical trials,DC-based immunotherapy as well as other tumor immunotherapy strategies have gained more significant progress.

Objective To investigate the resistance of clinically isolated bacteria to commonly used antibacterial drugs in the First Affiliated Hospital of Chongqing Medical University .Methods The bacterial susceptibility testing in clinically isolated bacte‐ria collected during January to December 2014 was carried out .The detection results were judged according to the standards by CLSI in 2014 .Results Among 7 740 clinical isolated strains of bacteria collected during this period ,Gram negative bacteria and Gram positive bacteria accounted for 70 .6% and 29 .4% respectively .Methicillin resistant(MR) strains in S .aureus and coagulase negative Staphylococcus accounted for 30 .2% and 77 .2% respectively .The resistance rates of MR strains to main antimicrobial a‐gents were much higher than those of methicillin sensitive(MS) strains .No staphylococcal strain was resistant to vancomycin or lin‐ezolid .The resistance rates of E .faecalis strains to main antibacterial agents was much lower than those of E .faecium .Some strains (2 .3% ) of E .faecium were found resistant to vancomycin ,while some strains(0 .9% ) of E .faecalis were found resistant to linezol‐id .The ESBLs producing strains were 59 .5% in Escherichia coli and 31 .8% in Klebsiella pneumoniae .Strains of Enterobacteriaceae were highly susceptible to imipenem and meropenem ,the overall resistance rates being less than 2 .0% .Resistance rates of P .aerug‐inosa to imipenem and meropenem were 24 .5% and 17 .9% ,respectively .The resistance rates of A .baumanii to the two carbapene‐ms were 71 .9% and 75 .0% ,respectively .The multi‐drug resistant K .pneumoniae and A .baumanii were increased markedly .Con‐clusion Bacterial drug resistance is serious ,especially the multi‐drug resistant bacteria constitute a serious threat to clinic .There‐fore it is urgent to strengthen the infection control measures .

Objective To establish an effective method of isolating and culturing circulating fibrocytes from human peripheral blood and study the relationship between the expression specific molecule markers expression and the morphological characteristics. Methods Total peripheral blood leukocytes were isolated from human peripheral blood by being centrifuged over Ficoll-Paque and cultured in DMEM supplemented with 20% fetal calf serum.Adhered cells were detected with immunocytochemistry,FCM and electron microscopy.The collagen synthesis was studied by measurement of the hydroxyproline concentration in the medium with chemical method. Results After 9 days cultue in vitro,CD34,CD45 and collagen Ⅰ staining was positive and 83.5% of these cells secreted collagen Ⅰ detected by FCM.Electron microscopy of circulating fibrocytes showed morphological characteristics of fibroblasts.The hydroxyproline concentration in the medium was 11.17%mg/L,which was statistically and significantly different compared with 8.07mg/L in the control medium(P

Scalp acupuncture is a method of treating whole-body diseases. The author takes the easy positioning of scalp acupuncture as starting point, covers the positioning of scalp acupuncture and needle insertion points, acupuncture manipulation and the selection of acupoints, so as to introduce the design of teaching the international standardized scalp acupuncture with texts and illustrations. The positions of scalp acupuncture are 4 lines in frontal area, 5 lines in parietal area, 2 lines in temporal area and 3 lines in occipital area. The needle insertion angle is 30° to the skin. Acupoints can be selected crossly and correspondingly in clinic.
Acupuncture ; education ; Acupuncture Points ; Acupuncture Therapy ; instrumentation ; methods ; Humans ; Scalp ; anatomy & histology

Objective: To build the relationship model of job burnout, insecurity psychology, and insecurity behavior for miners. Methods: The valid random samples included 1229 miners from national and private coal mines, who were investigated with the revised Job-Burnout Inventory for Miners, Insecurity Psychology Inventory, and Insecurity Behavior Inventory. The data was analyzed through SPSS15.0 and AMOS7.0. Results: The three dimensions of job burnout had significant direct effect on insecurity behavior [β =0.008, 0.047, 0.019) .The three dimensions of job burnout had significant direct effect on psychological paralysis (β =0.031, 0.203, 0.374) and helplessness on security (β =0.206, 0.374, 0.472) . Emotional exhaustion and disengagement from work had significant effect on temporary psychology (β =0. 192, 0.411) and antagonistic psychology (β =0.079, 0.596) . Four dimensions of insecurity psychology had significant effect on insecurity behavior (β = 0.092, 0.023, 0.093, 0.097) and it was a mediator in the model. Conclusion: Miners'job burnout have significant direct effects on insecurity behavior, but also have indirect effects through the insecurity psychology mediator.