Asthma is a chronic airway inflammation involved by a variety of cells.Glucocorticoid can relieve most of asthmatic patient's symptom,but cannot treat all patients with asthma.KCa3.1 ion channel expressed in a variety of immune cell surface, participate in a variety of autoimmune disease immune process.Because potassium-calcium ion channels are involved in the immune process mediated by T lymphocytes, the adjustment of the function of KCa3.1ion channels may be a new way for asthma treatment.

Airway remodeling is the result of chronic inflammation, which including airway wall thickening, matrix and collagen deposition, epithelial hyperplasia and fibrosis, smooth proliferation and hypertrophy,fibroblast proliferation, and mucus glands and goblet cell proliferation, micrangium generation and other pathological changes. Airway smooth muscle change is known as the reason of airway hyper - responsiveness and asthma aggravating. There are many factors which can induce airway smooth muscle hypertrophy and proliferation, such as inflammation, cytokines,extracellular matrix and genetic factors. In addition, recent researches reveal the airway smooth muscle is also an important source of inflammation. In this paper the latest opinion of the role of asthma airway smooth muscle in the airway remodeling were elaborated,and inhale hormone earlier was suggested.

The damage of airway epithelial cell in asthma including epithelial cells differentiated into goblet cell, mucous cells metaplasia, which lead to the extracellular matrix increasing, airway walls fibrosis and mucus high secretion. There are many cytokines, growth factors, signal transduction pathways and gene regulating this process.Neuroendocrine cell plays a very important role in the immune adjustment.The repairing process of airway epithelial cells after damaged is a complicated process and affected by many factors.

Objective To study the effect of the ECV-304 cells modified with LIF gene on the ex vivo culture of HSC/HPC in cord blood.Methods The ECV-304 cells were infected by Eukaryotic Expression plasmid pcDNA3.0LIF,and the positive ECV-304 cells were obtained by selected with G418.These cells were used to co-culture with CD34+ cells of cord blood.The phenotype of CD34+ and CD34+ CD54+、CD34+ CD62L+ primitive progenitors was detected by flow cytometry.Results The LIF gene can express in ECV-304 cells steadily.ECV-304 cells modified with LIF gene can improve expansion of CB CD34+、CD34+CD54+ and CD34+ CD62L+ cells while sustaining the expression of homing-related adhesion molecule.Conclusion The ECV-304 cells modified with LIF gene can not only significantly expand CB hematopoietic progenitor cells ex vivo,but the expanded CD34+ cells may well retain their homing ability.

There was never a time in history that so many disciplines and specialists participate and try to lead the clinical management for acute pancreatitis. Chaos remains at bedside, and episodes of under-intervention and especially over-treatment frequently happened. Physicians are easily confused by questions and controversies in management for acute pancreatitis. We have reviewed the articles in acute pancreatitis and made our recommendations based on the latest evidences.

Objective To evaluate the efficiency and safety of non- nasogastric tube on fast-track surgery patients with colorectal cancer. Methods We searched PubMed, CBM, Cochrane Library, CNKI, Wanfang and Vip data. Controlled trials that met the criteria were selected. All reports were collected and analyzed by RevMan5.3. Results 8 reports were enrolled. The non-nasogastric tube group had a shorter anus exhausting time and lower morbidity of pulmonary and throat complications , and it also had a shorter postoperative length of hospital stays. Significant differences were found between the non-nasogastric tube group and the control group (P< 0.01). There are no significant difference in the morbidity of gastrointestinal complications (P > 0.05). Conclusion Current evidence shows that non-nasgastric tubes are recommended for the patients with colorectal cancer surgery.

The incidence of hyperuricemia and relevant diseases has been increasing recently since the living improvement and dietary changing. Both patients and doctors do not pay enough attention to this disease, due to the lack of obvious clinical presentations in early stage. This paper comments on the relationship between hyperuricemia and gout, gouty nephropathy, impaired glucose metabolism,and atherosclerotic diseases in order to arouse enough attention to this disease.

Objective To establish a method for content determination of hyodeoxycholic acid in Qingkailing Injection. Methods An UltimateXB-C18 column (5 ?m, 250 mm?4.6 mm) was used with a mobile phase of acetonitril-water-phosphoric acid (35∶65∶0.1). The flow rate was 1 mL/min. The temperature of the column was 40 ℃. The detection wavelength was 192 nm. Results There was a good linear relationship between the concentration of hyodeoxycholic acid and absorption area value in range of 0.201 35～1.006 75 mg/mL, r=0.999 6. The average recovery was 98.25% with RSD=1.26%. Conclusion This method was accurate, credible and repeatable which can be used to control the quality of Qingkailing Injection.

Objective To observe the mRNA expressions of ornithine decarboxylase(ODC), endostatin and microvessel density (MVD) in esophageal squamous cell carcinoma (ESCC) and their correlations, and to explore the mechanisms of ODC in facilitating angiogenesis, its correlation with invasion a nd metastasis of the carcinoma. Methods Semi-quantitative RT-PCR method was used to detect the mRNA expressions of ODC and endostatin in 41 paired surgically resected specimens of ESCC (T)and their adjacent tissue(N), a nd the T/N ratio was calculated.Immunohistochemistry was used to detect the MVD by anti-CD 34 monoclonal antibody in ESCC tiss ue and corresponding adjacent tissue. Results Of 41 cases, the T/N ratio of ODC mRNA was greater than 1.0 in 39 cases (95.1%) and the T/N ratio of MVD was greater than 1.0 in 4 0 cases ( 97.6%). The mRNA expressions of ODC and endostatin, and MVD in esophageal specimens we re significantly correlated with tumor sizes, lymph node metastasis and adventit ia invasion. The mRNA expressions of ODC and endostatin were correlated with differentiation status of tumor. T/N value of ODC mRNA was positively correlated with T/N value of MVD, and nega tively correlated with the T/N value of endostatin mRNA. While the T/N value of endos tatin mRNA was negatively correlated with the T/N value of MVD. Conclusion ODC is closely related to tumor angiogenesis, invasion and metastasis of ESCC. It is possible that ODC overexpr ession may promote tumor angiogenesis by suppressing endostatin expression, which may be o ne of the mechanisms that ODC facilitates invasion and metastasis of ESCC.

OBJECTIVE:To investigate release characteristics of sirolimus liposome in vitro.METHODS:The concentration of sirolimus was determined by RP-HPLC.In vitro release rate of sirolimus liposome within 24 h was investigated by the reverse dialysis method with 20% ethanol 500 mL as medium.Release curve of sirolimus was fitted with drug release model equation.RESULTS:The linear range of sirolimus were 0.5～20 ?g.mL-1(r=0.999 8)with an average recovery of 99.42%(RSD=1.23%).At the first 4 hours of release,sirolimus liposome released rapidly with accumulative release rate of 50%.After that release rate of liposome was slowed down with accumulative rate of 80% in 24 h.The in vitro release curve conformed to the first order equation.CONCLUSION:Sirolimus liposome has delayed release capability,and in vitro drug release of sirolimus liposome is in concentration dependant manner.