OBJECTIVE To study the influence of enzyme detergent for improving the quality during the cleaning of medical instruments.METHODS The 360 pieces of contaminated instruments for manually cleaning were divided into two groups with 180 pieces each, one group as test one immersed in Lionser enzyme solution 5 minutes at 1∶200 and another one as control immersed in non-enzymatic detergent 30 minutes at 1∶200.Eventually flush all instruments using free water.RESULTS Significant difference(P

Strontium-89(89Sr)therapy has been recognized as an effective method for treating bone metastases. It functions as a calcium analog that binds with collagen matrix produced by the MC3T3-E1 cells during collagen mineralization. 89Sr emits pure β rays which palliate bone pains and reduce the number of new metastasis sites. Response to 89Sr is affected by factors such as the location, size and histological type of lung cancer, the type of metastasis and the duration of treatment, and can be predicted by relief of bone pain, elimination of metastasis, CD4 +/CD8 +, ALP, and others. 89Sr therapy is safe and effective. Repeat administration or combined used with other treatments can improve the therapeutic effect of 89Sr.

Objective To explore the clinical features,treatment methods and effects of late-stage burned complicated with stress ulcer.Methods The clinical data of 206 burned patients were retrospectively analyzed,and the clinical features,treatment methods and effects of burned patients complicated with stress ulcer were analyzed.Results In 206 patients,there were 42 late-stage burned patients complicated with stress ulcer(20.4％),of which 34 cases were cured(81.0％),8 cases died(19.0％) (3 cases died of stress ulcer bleeding,5 cases died of stress ulcer bleeding complications).Conclusion The probability of late-stage burned complicated with stress ulcer is high and dangerous.For the patients with late-stage burned patients complicated with stress ulcer,early enteral nutrition,gastric mucosal protection,wound sepsis to prevent wound infection,the treatment measures should be effectively put into practice,which will be good for making patients stable,and then the patients can be cured.

Objective :To establish a simple, reproducible brain edema model induced by endotoxin in infant rats and explore the role of intracellular free calcium and calmodulin (CaM). in the development of brain edema. Methods: A total of 45 infant rats were divided randomly into endotoxin treated( n = 36) and control( n = 9) group. The animals were intraperitoneally injected with 10mg/kg of endotoxin or saline respectively. The brain tissue intracelluar free [Ca2 + ] as well as the expression of CaM was detected by fluorescence and protein hybridizaton methods respectively. The tissue water content,Evans Blue content and pathology under electronic microscope were also observed. Results:Brain water and EB content were remarkably increased in endotoxin treated animals compared with those in the control, in which the intracellular free [Ca2+ ] was also significantly increased and the expression of CaM up - regulated. Electron microscopic studies revealed that the blood - brain barrier(BBB)was disrupted with neuronal degeneration,neurogliocyte swelling and necrosis. Conculsion :These results suggest that LPS increases the intracellular free [Ca2+ ] ,and activates the CaM - dependent function via Ca2+ /CaM pathway,which might relate to the increased BBB permeability and brain edema formation.

ObjectiveTo observe the effect of Sanfeng Rougan Tang on the type of Ganyang Shangkang essential hypertension.Methods42 essential hypertension patients in type of Ganyang Shangkang Zhen were treated with Sanfeng Rougan Tang. The anti-hypertension medicine remained if the patient had taken. The blood pressure and symptoms of the patients was recorded before and a month after treatment.ResultsAfter treatment, the blood pressure and clinical symptoms of the patients had been significantly improved. The ratio of the blood pressure control was 95.2%, and of the primary symptoms control was 94.4%.ConclusionSanfeng Rougan Tang is effective on the type of Ganyang Shangkang essential hypertension.

ObjectiveTo investigate the effect of psychological intervention on quality of life (QOL) of patients with mammary carcinoma.Methods64 patients with mammary carcinoma were divided into intervention group (n=36) and control group (n=28), and psychological status and QOL of patients of two groups were analyzed after treatment.ResultsThe psychological status and QOL of intervention group was better than that of control group (P<0.05～0.001).ConclusionThe psychological intervention in full stage being in hospital can improve QOL of patients with mammary carcinoma, and it is clinically significant to improve cure rate and survival rate of patients.

Objective To compare the clinical effects of low-temperature plasma ablation combined with adenoidectomy and decolle-ment in the treatment for patients with sleep apnea syndrome.Methods Totally 68 patients with sleep apnea syndrome who underwent elec-tive surgical treatment in our hospital from June 2013 to February 2015 were selected as the study objects.According to the order of treat-ment,the patients were randomly divided into the control group and the observation group,with 34 cases in each group.The control group were treated by routine decollement.The observation group were treated by low-temperature plasma ablation combined with adenoidectomy.After operation,the 2 groups were followed up for 6 months.The improvement effects of different operative methods in clinical symptoms and prog-nosis of patients with sleep apnea syndrome were compared.Results The total effective rate in the observation group (97.06%)was higher than that in the control group (82.35%),and the difference was statistically significant (P <0.05).The postoperative VAS pain grading of the observation group was relatively lower.The normal proportion (50.00%) was significantly higher than that in the control group (11.76%).Patients with moderate and severe pain in the control group accounted for 44.12% and 14.71% respectively,which were signif-icantly higher than those in the observation group(P <0.05).The incidence of postoperative bleeding in the observation group(2.94%)was significantly lower than that in the control group while the incidence of fever was higher than that in the control group (P <0.05).After oper-ation and at the end of the follow-up,OSA-18 score of the observation group decreased significantly and were (46.66 ±7.87)and (33.47 ± 10.59)respectively.The decreased range was greater than those in the control group (P <0.05).Conclusion To adopt low-temperature plasma radiofrequency ablation combined with adenoidectomy in the treatment of patients with sleep apnea syndrome can improve the surgical treatment effect,reduce postoperative pain,reduce the incidence of postoperative bleeding,optimize patients’quality of life and improve the prognosis.

Objective To investigate the role of subdural puncture(SDP)in the diagnosis and treatment of subdural fluid collection in young children with purulent meningitis.Methods Totally 207

Objective To investigate the distribution and antibiotic resistance of pathogenic bacteria in PICU and to provide evidence for use of antibiotics rationally.Methods All the basic clinical features and pathogenic bacteria isolated from patients in PICU of our hospital from October 2009 to September 2012 were analyzed retrospectively.Results A total of 672 pathogenic bacterial strains were isolated (positive rate 16.7％ 672/4 020),75.1％ and 24.9％ were gram negative bacteria and gram positive bacteria,respectively.Pseudomonas aeruginosa was the most common bacteria isolated in gram negative bacteria,followed by Klebsiella pneumonia and Escherichia coli in this order.And Staphylococcus was the main strain in gram positive bacteria.Positive specimens were mainly from lower respiratory tract and bloodstream infection.Most Staphylococcus were resistant to penicillin,oxacillin and erythromycin but susceptible to vancomycin,teicoplanin and linezolid.Gram negative bacteria were resistant to ampicillin,ampicillin-sulbactam,piperacillin and cephalosporin,but susceptible to imipenem,cefoperazone-sulbactam,piperacillin-tazobactam,ciprofloxacin and amikacin.Conclusion Gram negative bacteria is the prevalent strains in PICU of our hospital,Pseudomonas aeruginosa,Klebsiella pneumonia and Escherichia coli are common bacterium isolated.Most isolates are highly drug-resistant.Analysis of pathogenic bacteria and drug-resistance surveillance are of vital importance to guide treatments for critically ill children and reduce drug-resistant bacterial strains.

Objective To identify factors influencing behavioral changes among injection drug users(IDUs)by assessing the outcome of the Kunming Sunshine Homeland Project(SHP)in 2007,so as to design more effective and relevant interventions for HIV/AIDS prevention and control in China.Method Data obtained from SHP were analyzed by Logistic regression,in which the relationship between related factors and effectiveness of the interventions were examined.Results Based on Fishbein and Guinan’s hypothesis(1996),among the three factors,i.e.attitude,perceived behavioral norm and self-efficacy,the perceived behavioral norm was the most effective one to change IDUs’ behaviors.Conclusions In designing HIV/AIDS prevention and control projects targeted to IDUs,perceived behavioral norms should be given high priority in combination with other 2 factors,and the approaches applied should match with the objective of the designed project.