Objective:To explore mental health of students of military medical university Method:Based on understanding of help seekers in counseling, we selected grade-2 students in the fourth military medical university as our sample (n=374, male: 252, female: 122; 264 from army, 110 submitted by civil units) The research instruments included SCL-90 and EPQ Results:EPQ results showed, female had lower N, P scores, higher E score than male They also had higher score of phobia in SCL-90 than male, while male had higher scores of obsession, interpersonal sensitivity, and anxiety in SCL-90 than female Compared with students from one child family, students having siblings had higher P score, lower E score, and higher scores of depression, phobia, and psychotism in SCL-90 Civil students had lower N, P scores and higher E score than military students did They had also lower scores of obsession, interpersonal sensitivity, depression, anxiety, and psychotism in SCL-90 Conclusion: In students of military medical university, male, those from multiple children families and those from army have relatively poor mental health than their counterparts

Objective To evaluate availability and outcomes of using anterior half of the peroneus longus tendon (AHPLT) in knee ligament reconstruction as an autograft source.Methods From July 2007 to January 2008,100 patients with knee ligament injuries were recruited in this study.There were 33 males and 67 females aging from 16 to 62 years (mean,32.3 years).49 cases had undergone medial patellofemoral ligament reconstruction,19 cases multiligament reconstruction,18 cases double-bundle posterior cruciate ligament (PCL) reconstruction and 14 cases double-bundle anterior cruciate ligament (ACL) reconstruction.AHPLT was used as sole (49 cases) or part (51 cases) of reconstruction materials.One-incision or two-incision striping techniques were adopted to harvest AHPLT.Ligaments were fixed with screws.Post-operative assessments included Kujala knee score,Lysholm knee score,Marx knee score,International Knee Documentation Committee (IKDC) subjective evaluation form and objective evaluation grade,the Foot and Ankle Disability Index (FADI) and the American Orthopedic Foot and Ankle Society (AOFAS) scale.Results 92 cases were followed up for more than 24 months.Postoperative Kujala score,IKDC subjective score,Lysholm score and Marx score were improved significantly in all four groups of patients.According to IKDC objective evaluation grade,the number of patients reaching Grade A (normal) or Grade B (near-normal) in multiligament,PCL and ACL reconstruction were 17,15 and 12,with an excellent rate of 89.5％ (17/19),93.7％ (15/16) and 100％ (12/12),respectively.Preoperative and postoperative AOFAS scores were 97.4±2.0 and 97.2±1.6,respectively,while the FADI scores preoperatively and postoperatively were 96.8±2.2 and 96.9±2.5,respectively.These results had no statistical significance.No signs of peroneal nerve injury or peroneus longus tendon rupture was found.Conclusion It is acceptable to use AHPLT as an autograft due to its feasibility to harvest,good clinical outcome,and low rate of donor site morbidity at a minimum of two-year follow-up.

ObjectiveTo evaluate the influence of chronic obstructive pulmonary disease on the risk of intrathoracic operation.Methods35 patients associated with chronic obstructive pulmonary disease undergoing intrathoracic operation were analyzed retrospectively.ResultsPostoperative acute and chronic respiratory failure occurred in 4 patients respectively, resulting in 6 deaths.ConclusionThe indication of patients associated with chronic obstructive pulmonary disease for intrathoracic operation should be critically scrutinized, which may include FVC>60% and MVV>40% or FEV1>40%.

AIM: To explore the biopharmaceutical character of the combination of Fructus Schisandrae and Radix Salvia Miltiorrhizae. METHODS: Mice were divided into four group according to schizandrol A, Schizandrin B, Fructus Schisandrae and combination with Radix salvia Miltiorrhizae and then perfused. Mouse's bood plasma was collected in sequence, and the test solusion was prepared by adding amine sulfate and the mixture of acetonitrile methanol (4∶1). Its contents were measure by HPLC in the aid of SIP and 3p97 program. RESULTS: Metabolic rate in mouse of schizandrol A and schizandrin B from combination is apparently lower than that of non combination. CONCLUSION: Chinese Traditional medicinal combination has its biopharmaceutical value.

Objective:To develop an interface program between a hospital inspection information system and Hospital Information System(HIS). Such interface program not only guarantees the safety and independence of HIS, but achieves interoperability between HIS and other third-party system.Methods: The interface program between the inspection information system and HIS conforming to hospital demands is developed by using Visual Studio 2008 development platform and WebServices+XML technology.Results: The interface between the inspection information system and HIS operates stably, reliably and efficiently, avoiding manually input information errors, reducing the amount of registration work, and raising work efficiency.Conclusion:WebServices+XML technology is utilized to develop an interface characterized by safety, stability and reliable performance, realizing resource sharing among information systems in the hospital. Besides, the interface can be expanded in the future to adapt to other third-party system, achieving customization.

Objective To evaluate the choice of therapeutic methods and MRI diagnosis of acoustic neuroma.Methods The MRIimaging and clinical materials of 60 patients with acoustic neuroma were analyzed,48 cases were underwent an operation,12 cases weretreated conservatively or gamma knife treatment,follow-up ranged from 1 to 4 years.Results There were 62 tumors round the internalauditory canal.There were 58 cases with single acoustic neuroma and two cases with couples acoustic neuroma.28 tumors demonstratedhypointense and 30 tumors demonstrated hypo-and isointense signals on T_1 weighted image.38 tumors demonstrated hyperintense and 24tumors demonstrated hyper-and isointense signals on T_2 weighted image.The Ⅶ,Ⅷ nerves affected side were thickened than that ofopposite side in 32 patients.After Gd-DTPA administration 24 tumors were homogeneously enhanced,26 tumors were inhomogeneously or circularly enhanced in 50 acoustic neuromas of 48 cases.Operation was still the main election for acoustic neuroma.Conclusion MRI is an effective method in the diagnosis of acoustic neuroma,and providing advice for clinics in making therapeutic programs.

Polymerase chain reaction(PCR) was employed to amplify the whole HBV X region from the serum of patients with chronic hepatitis B virus(HBV) infection, and then the PCR products were subcloned into pGEM Teasy vectors. Clones were randomly selected to be sequenced. Comparison of the cloned sequence was made to look for the differene. The sequencing results showed that each sequence of selected clones was differenct and there were HBV quasispecies groups in patients. There was hot deletion region near the 3' end of X gene. To address whether the mutations were responsible for the transactivating effect,the wild type(wt) and the mutants of HBV X genes were subcloned into the EcoRI site of pcDNA3 1( ) vectors, and the recombinant plasmids were cotransfected into HepG2 cells with reporter plasmid pSV lacZ,respectively. The activity of ? galactosidase controlled by SV40 early promoter/enhancer was detected, which reflected the transactivating function of HBx protein. The cotransfection results indicated that the wt HBV X gene acted as a transactivator on the SV40 early promoter/enhancer, and the mutations which caused premature termination of the X open reading frame reduced their transactivating effects to certain extent.

AIM: To study the cytoprotective role of NaNO_2 preconditioning against H_2O_2 induced damage in PC12 cells. METHODS: PC12 cells were treated with different concentrations of NaNO_2 for 6 h, 12 h, 24 h and 48 h, respectively. The viability of the cells was measured by MTT method and cell counting. The apoptotic rate of PC12 was determined by Hoechst 33258 staining to calculate the ratio of the cells between concentrated and broken nucleus in the total cell count. PC12 cells were pretreated with NaNO_2 at concentration of 3 mmol/L for 24 h. The cytoprotective role to the toxicity of H_2O_2 at concentration of 1.1 mmol/L for 6 h was observed by MTT. The cell apoptosis was measured by flow cytometry and staining with Hoechst 33258 and PI. The activities of catalase (CAT), superoxide dismutase (SOD), and the changes of glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) were measured by the method of colorimetry. RESULTS: The dose-response results showed that the effect of NaNO_2 on PC12 cell proliferation was a typical β-shape curve. The maximal stimulatory response was at 24 h, and the concentration of the maximum stimulatory response was 1.4 mmol/L. The maximal stimulation of the concentration-responses was 156% above the control. No observable adverse effect level (NOAEL) was 6 mmol/L. IC_(50) was 45 mmol/L. When the cells were pretreated by NaNO_2 at concentration of 3 mmol/L for 24 h, and then exposed to H_2O_2 at concentration of 1.1 mmol/L for 6 h, the proliferation rate was increased as compared to the cells treated with H_2O_2 alone. Under the conditions of treating the cells with NaNO_2 at concentration of 3 mmol/L to induce the adaptive response, then exposing the cells to H_2O_2 at concentration of 1.1 mmol/L, the apoptosis rate in non-preconditioning group was 44.9%, the apoptosis rate in preconditioning group was 19.1%, the difference was significant (P<0.05). The cytoprotective effect of NaNO_2 was inhibited by nitric oxide (NO) scavenger ferrohemoglobin. The activities of SOD, CAT and the level of GSH-Px were markedly increased, the content of MDA decreased in preconditioning group. CONCLUSION: Exposure of NaNO_2 at concentration of <6 mmol/L induces hormesis on PC12 cells. Low dose of nitrite plays an important role in cytoprotection by reducing nitrite to NO, indicating that decrease in lipid peroxidation and increase in endogenous antioxidants may play a key role in cytoprotection induced by preconditioning with low dose of NaNO_2 in PC12 cells.

This study is to investigate the cytoprotective role of NaNO2 preconditioning against ethanol induced damage in human hepatoma SMMC-7721 cells. The cells were preconditioned with NaNO2 (0.25 mmol x L(-1)) for 24 hours or 4 weeks, and then exposed to ethanol (200 mmol x L(-1)) for additional 12 h and untreated cells served as control. Both temporal and chronic NaNO2 preconditioning could prevent ethanol elicited cytotoxicity as evidenced by thiazolyl blue (MTT). NaNO2 preconditioning also could inhibit ethanol-induced apoptosis, which was confirmed by FITC-Annexin V/PI flow cytometer and Hoechst 33258 and PI staining. Further, simultaneous NaNO2 preconditioning treatment along with ethanol showed protection against ethanol mediated cellular damage as indicated by significantly decreased levels of malondialdehyde (MDA) and elevated activities of superoxide dismutase (SOD) and catalase (CAT). Western blotting analysis revealed that in ethanol treated cells preconditioned with NaNO2, the HIF-1alpha and Bcl-2 increased obviously, while the expression of pro-apoptotic proteins, including Bax, Caspase-9, Caspase-3 decreased. The results showed that low doses of NaNO2 preconditioning resistant to ethanol-induced human hepatoma SMMC-7721 cells apoptosis, which mechanism may be related to increased expression of HIF-1alpha in the cells.