Objective To study the expression of red blood cell keratin 19(CK19) mRNA in the peripheral blood of the pa‐tients with colorectal cancer and the relationship between the CK19 mRNA and the pathological characteristics ,to investigate the relationship between CK19 and colorectal cancer .Methods Clinical data of patients with colorectal cancer received treatment at our hospital from 2012 to 2014 was retrospectively analyzed .A total of 160 patients were retrospective analyzed ,including 52 patients with colorectal cancer ,55 with colon polyps and 53 normal persons .The control group was the healthy subjects and the patients with colon polypsc who accept the physical examination at our hospital at the same period .The different expressions of CK19 mR‐NA of colorectal cancer ,colon polyps and the normal persons were compared .The relationship between the CK19 mRNA and the pathological characteristics of colorectal cancer was analyzed .The different diagnostic values of CK19 mRNA and CEA ,CA199 to colorectal cancer were compared .Results There were significant differences in the positive rate of CK19 mRNA between three groups(χ2 =54 .53 ,P<0 .01) .The positive rate of CK19 mRNA in the patients with colorectal cancer was the highest .The positive CK19 mRNA in the patients with colorectal cancer of different Dukes typing(χ2 =16 .14 ,P<0 .01) ,differentiation(χ2 =8 .155 ,P=0 .017) ,liver metastasis(χ2 =13 .68 ,P<0 .01) were different .And the differences were significant .The result of ROC area under the curve was 0 .947 which described the diagnostic value of CK19 mRNA to the patients with colorectal cancer .The sensitivity and specificity of CK19 mRNA were higher than both of CEA and CA199 .Conclusion The expression of CK19 mRNA in colorectal cancer is significantly increased .There is significant correlation between the expression level of CK19 mRNA and the staging ,grad‐ing and transferring of the tumor .The diagnostic value of CK19 mRNA to the patients with colorectal cancer is better than CEA and CA199 .

Mucopolysaccharidosis type Ⅱ is a rare single gene inherited disorder. In the earlier studies, more attention was paid to the molecular analysis of the hot spots of the mutations. With the detection of more and more novel mutations,more studies are trying to analyze the effect on splicing of different types of mutations and the genotype-phenotype correlations. This review will introduce the progress on molecular genetics, the gene mutation,analysis of the female patients and the genotype-phenotype correlations. With the study of these progress,it suggested that more attention should be paid to the possibility of gene-pseudogen recombinations, trying to study the effect on splicing from the cDNA level, which will benefit the investigation of the genotype-phenotype correlations.

Objective: To study the relationship between endoplasmic reticulum stress (ERS) and adiponectin;to explore the role of ERS for increasing myocardial ischemic vulnerability in type 2 diabetes mellitus (DM) mice.
Methods: Type 2 DM model was established by high fat diet with streptozotocin (STZ) injection. A total of 35 C57BL/6J male type 2 DM mice were divided into 4 groups: ①Control group, n=5. ②Tauroursodeoxycholic acid (TUDCA) group,③Thapsigargin (TG) group and ④Normal saline group. The mice in Groups ②, ③, ④were fed by high fat and high glucose diet by injecting streptozotocin (STZ), in the last 3 weeks and respectively received intraperitoneal injections of TUDCA (250 mg/kg), thapsigargin (TG) (300μg/kg) and normal saline twice a day, n=10 in each group. Then myocardial infarction (MI) model was established in 5 mice from each group. 72 hours later, the MI ranges were measured, serum levels of adiponectin were detected, mRNA expressions of adiponectin and CHOP in myocardial tissue were examined.
Results: The MI range in TUDCA group (21.47 ± 2.85)%and in Normal saline group (39.92 ± 4.28)%were both lower than TG group (66.56 ± 8.15)%, both P<0.01. Before MI occurrence, serum levels of adiponectin in TUDCA group (79.25 ± 6.40) pg/ml and in Normal saline group (70.23 ± 4.15) pg/ml were both higher than TG group (62.64 ± 5.70) pg/ml, both P<0.01;serum levels of adiponectin in each group were higher than they were 72 h after MI. In each group, the mRNA expression and protein content of adiponectin in myocardial tissue were constant to serum adiponectin;while the mRNA expression and protein content of CHOP was opposite to serum adiponectin.
Conclusion: ESR could increase myocardial vulnerability in type 2 DM mice which might be related to down-regulating adiponectin expression.

A variety of molecular markers are related to the prognosis of gastric cancer,such as the the loss expression of AT rich interactive domain 1A (ARID1A),the overexpression of CD133 and survivin are releated to the incidence and poor prognosis of gastric cancer; leptin,CD44 and microRNA (miRNA) are releated to the invasion and metastasis of gastric cancer; the low expression of secreted protein acidic and rich in cysteine (SPARC) contributes to the angiogenesis of gastric cancer,but the relationship between SPARC and prognosis needs further study; the expression and gene polymorphism of excision repair cross-complementing 1 (ERCC1) may be useful for choice of medication in the treatment of gastric cancer.

Objective:To compare the diagnostic value of conventional fine needle aspiration biopsy(C-FNAB) and Ultrasound-guided FNAB(USFNAB) for thyroid nodules by Meta-analysis.Methods: PubMed and CNKI database were searched for English and Chinese language articles related to FNAB diagnosis for thyroid nodules.The literatures were assessed and screened according to the Standards for Reporting of Diagnostic Accuracy(STARD).Data were pooled by Metadisc software.Results: A total of 8 810 specimens were included in conventional FANA group and the pooled sensitivity,specificity,and the AUC of weighted summary receiver operating characteristic(SROC) curve were 84.9%,76.3% and 0.919 4,respectively;2 393 specimens were included in US-FNAB group and the above numbers were 84.0%,80.8% and 0.935 6,respectively.Conclusion: FNAB has high accuracy in diagnosing thyroid nodules;the accuracy of ultrasound-guided FNAB is higher than conventional FNAB.

The pro-inflammation cytokine is secreted by cells,and the function is to promote and regulate inflammatory reactions.They include IL-1,IL-6,TNF-? and IFN-?.Researchs have revealed that the levels of serum pro-inflammation cytokinemight increase notably in inflammatory bowel disease patients and they could cause the change of intestinal epithelium tight junctions.

Objective The present study was to analyze the role of ACh-lateral septum(SL)pressor system in the Central amygdaloid nucleus(AC)-emotional pressor circuit.Method Interurban microinjection of different drugs,then blood pressure and heart vate were vecorded.Results(1)CRF(corticotropin releasing factor)or SP(substance P)microinjection into the SL can induced pressor responses.The AC pressor responses to glutamate(Glu)could be attenuated by preinjection of CRF antagonist or SP antagonist into bilateral SL.(2)the AC pressor responses to Glu were also reduced by preinjection of atropine into either bilateral HBL,LC or RVL respectively,(3)Since lateral HB(HBL)projects to the posterior hypothalamus(HP)containing ACh-ergic neurons,and excitation of the latter produces pressor response via the RVL and LC-RVL;atropine preinjection into the HP could also decrease the HBL-and AC-pressor response.Conclusion The present results indicate that the AC produce pressor responses involved with the SL via their CRF and SP,mechanism underlying pressor response of SL is involved in pressor response of AC.

The study compared the effects of the branched-chain amino acid enriched solution with standard.amino acid solution on patients during early postoperative period.Sixteen patients who underwent mode 時ate abdominal operative procedures were divided into study (BCAA) and control groups randomly.Either group of patients received equal amount of nitrogen (0.2g/kg d-1) and energy(l46-167kJ/kg d-1) during the first five postoperative days.Recommended doses of other nutrients were added daily.Nitrogen balance was detemined every day postoperatively.Plasma albumin,fibronectin and amino acid profile as well as other parameters were monitored preoperatively and on 3rd and 6th postoperative days.The results suggested that the nutritional effects of the branched-chain amino acid enriched solution might be superior to that of the standard nutritional amino acid solution on postoperative patients.

The childhood asthma is characterized as high morbidity,recurrent attack,chronic persistence and refractoriness. So asthma with these features mentioned above can have important effect on children′s emo-tional and physical development as well as their families. This review sketches the studies of children asthma family management present situation and its influencing factors,and stresses the necessity of asthma family man-agement. Methods of psychotherapy used in our country and abroad and its implementation effect are reviewed, including behavior therapy,cognitive therapy,cognitive behavioral therapy,relaxation therapy,family therapy and other existing psychological treatment,in order that these measures can be conductive to the development of pre-vention methods of pediatric asthma and further researches.

Objective To observe the selective regulation of peroxisome proliferator-activated receptors (PPAR) on fatty acid binding protein-4 (FABP4) in human syncytiotrophoblasts.Methods Cultivate normal human syncytiotrophoblast cells,and put in the specific antagonists and agonists of PPAR each subtypes receptors,then observe the different expression of FABP4 mRNA and protein.Results Pretreated the human syncytiotrophoblast cells with the agonists (GW7647,GW0742) and antagonists (GW6471,GSK0660) of PPARα and PPARβ receptors,the expression of the FABP4 was not significantly change (P＞0.05).However pretreated with PPARγ agonists (rosiglitazone,1 × 10-9,1 × 10-8,1 × 10-7 and 1 × 10-6 mol/L),the expression of FABP4 mRNA and protein could be dose dependent-promoted significantly (mRNA:1.27 ±0.12,1.45 ±0.14,1.57±0.14,1.72 ±0.12,protein:1.10 ±0.08,1.37 ±0.09,1.60 ±0.13,1.79 ± 0.14 ; P ＜ 0.05),furthermore,the promotion can be dose dependent-reversed by specific antagonists GW9662 (mRNA:0.92 ± 0.06,0.77 ± 0.06,0.64 ± 0.05,0.55 ± 0.05,protein:0.91 ±0.03,0.78 ±0.06,0.70±0.07,0.55 ±0.06; P ＜ 0.05).Conclusions In normal human syncytiotrophoblast cells,FABP4 is a target factor of PPARγ.PPARγ regulated the expression of FABP4 mRNA and protein selectively.And the regulation will not be influenced by the other two PPAR subtypes.