Objective To discuss the effect of treating tubal obstruction infertility through hydrotubation by hysteroscopy combined with Chinese traditional medicine. Methods Chose 308 patients with tubal obstruction infertility from October 2001 to December 2008 and used the hysteroseopy under which making tubal cannulation, then injected methylene blue and clear liquid to record cases of tubal patency.After the operation the patients would be given Chinese traditional medicine and followed up the case of pregnancy. Result The recanalization rate in 308 patients was 90.9% (280/308), and the rate ofre-pregnancy was 55.5%(161/290). Conclusion The effect of treatment through hysteroscopy to make tubal cannulation and have patients take medicine after operation is very satisfactory and it is worth promoting.

〔Abstract〕 Taking PubReader as an example , the paper introduces typical applications of HTML 5 in displaying medical information . By cases in medical information display , it illustrates advantages of HTML 5 in interface alternation , intelligent terminal support , multime-dia presentation , and local storage , etc.It also predicts its future development tendency and disadvantages .

Neovascularization is the basis of tumor growth and metastasis. Angiogenesis depends mainly on the regulation of vascular endothelial growth factor and angiogenesis inhibiting factor. The vascular endothelial growth factor and its receptor are the most important and are studied most. The expression of them increased significantly in gastric cancer tissue and serum.. They promote angiogenesis of tumor. They are closely related to the prognosis of patient. The vascular endothelial growth factor and its receptor in the gastric cancer are reviewed in this article.

Objective To evaluate the efficacy of three-dimensional conformal radiotherapy(3D-CRT) combined with transcatheter hepatic arterial chemoembolization(TACE) for patients with primary liver cancer.Methods From July 2003 to July 2006,followed by transcatheter hepatic arterial chemoembolization(TACE,mitomycin + 5-fluorouracil + pirarubicin) 3 times in 6 weeks,59 patients with primary hepatic cancer received noncoplanarity multifield 3D-CRT by using Leibinger three-dimensional stereotactic conformal radiotherapy planning system,Varian 600C/D 6MeV X liner accelerator and multileaf collimator at a dose of 60-65Gy/9-11 fractions/15-22 days.Results Of 59 patients,58 cases received a complete course of 3D-CRT combined with TACE.CT results showed that the rates of complete response(CR),partial response(PR),no change(NC) and progression(PD) rate were 57.6%(34/59),35.6%(21/59),3.4%(2/59) and 3.4%(2/59),respectively.Overall response rate(CR+PR) was 93.2%(55/59).Jaundice occurred one week after treatment in one patient,who died of acute hepatic function failure.No serious adverse effects or intoxication were found in the remaining 58 patients.Conclusions The combination of 3D-RCT with TACE was an effective treatment for patients with primary hepatic cancer.

Objective To detect the creatin kinase (CK) and its isozeyme (CK-MB) activity in serum of the healthy black bear (Ursus thibetanus) reared in pens to establish reference standard of biochemistry for CK and CK-MB activities of black bears as well as to offer scientific basis for prevention and cure of the black bear disease. Methods The CK activity and CK-MB activity were measured using spectrophotometer based on the principle of enzymatic reaction rate. All data were used to calculate the mean value, variation coefficient and the confidential interval of expected value of enzymatic activity. Results Under 30 ℃, of the male black bears, the average CK activity is 162.70 U/L, the variation coefficient 11. 90%, the confidential interval of expected value 148.90 to 176.50 U/L, and the average CK-MB activity is 17.4 U/L, the variation coefficient 17%, the confidential interval of expected value 15.26 to 19.54 U/L. Of the female black bears, the average CK activity is 149.3 U/L, the variation coefficient 8%, the confidential interval of expected value 140.68 to 157. 92 U/L, and the average CK-MB active is 15.8 U/L, the variation coefficient 18.6%, the confidential interval of expected value 13.70 to 17.90 U/L. Conclusion The data we obtained can serve as biochemical indexes for the healthy black bears.

AIM: To optimize the best technique of making Semen Euphorbiae into frost-like powder. METHODS: Machine was developed equipped with temperature and pressure control. Pretreatment of Semen Euphorbiae, heating temperature and time were selected as factors,fat oil content,euphorbiasteroid and lathyrol diadetate benzozte as main levels,to evaluate the processing. RESULTS: The experiment showed that seeds of Semen Euphorbia were directly broken into powder and heated at 55 ?C for 30 min. The oil productivity ranged from 51% to 53% . CONCLUSION: The heating temperature has an influence on fat oil and laxative constitutents' content, whereas pretreatment of seed and heating time lack the relationship to.

Objective To investigate the biomechanical changes of internal maxillary distraction with fixation between cleft palate.Methods Three-dimensional FEM models of LeFort Ⅰ osteotomy and soft tissue in CLP maxillary complex were established.Two different conditions were simulated:one was the cleft palate connected by a titanium plate,and the other was the cleft retained.Internal midface distraction which applied in clinic were simulated.Fixation points of the distraction devices on the LeFort Ⅰ osteotomy complex were advanced by 10 mm in the direction of frontage.Biomechanical changes for different conditions were investigated by means of finite element analyses.Results LeFort Ⅰ osteotomy of maxillary complex area in CLP was advanced.Constrictions occurred in alveolar crest and palate,when the cleft was retained.Constriction of alveolar crest and palate was not obvious,when the cleft palate was connected by a titanium plate.There were no differences between sagittal and vertical displacement to internal distraction in LeFort Ⅰ osteotomy.Clockwise rotation occurred in vertical direction.Conclusions Internal maxillary distraction with fixation between cleft palate could avoid constriction of alveolar crest and palate.It provides the options and reference to the surgical plans.

BACKGROUND: Relapse of acute leukemia is possibly correlated with abnormal expression of cellular adhesion molecule-1 (VCAM-1).OBJECTIVE: To observe the level of VCAM-1 in serum and bone marrow stromal cell (MSC) supernatant of patients with relapsed acute leukemia. METHODS: Samples of serum and MSC supernatant were collected from 17 patients with remission-phase and relapse-phase acute leukemia hospitalized in the Department of Hematology, the Fifth Affiliated Hospital of Sun Yat-sen University between June 2006 and March 2008. The levels of VCAM-1 were measured with ELISA in remission and relapse phases.RESULTS AND CONCLUSION: Compared with the remission phase, the VCAM-1 level of serum was significantly increased in the relapse phase (P < 0.05). At 1 and 3 weeks after in vitro culture, the VCAM-1 level in the MSC supernatant was not changed (P > 0.05), but it was significantly increased on the fourth week (P < 0.05). The results demonstrated that VCAM-1 expression of serum and MSC supernatant in the relapse phase was greater than that in the remission phase, suggesting that abnormal expression of VCAM-1 was possibly correlated with relapse of acute leukemia.

OBJECTIVE: To prepare clonazepam oral disintegrating tablets and to evaluate its quality. METHODS: Gelatian and aspartame were used as tastes masking. Microcrystalline celluose, low substituted hydroxypropylcellulose and polyvininylpolyrrolidone were used as disintegrants. Clonazepam orally disintegrating tablets were prepared by wet granules. The in vitro and in vivo disintegration time, the relationship between hardness and disintegration time and the relationship between the usage of magnesium stearate and disintegration time were investigated, while taste and dissolution rate were also evaluated. RESULTS: When the gelatian/aspartame/poloxamer ratio was 30:0.5:1, the tablets had good oral feel and the uppermost dissolution rate. The dissolution rate of clonazepam oral disintegrating tablets which was evaluated by the method of common tablets could exceed 95% after 20 minutes. When the microcrystalline celluose / low substituted hydroxypropylcellulose / polyvininylpolyrrolidone ratio was 9:1: 3, the in vitro and in vivo disintegration time Was within 30 s. When the usage of magnesium stearate was 0.5% and the hardness ratio was in the range of 3-4 kg, disintegration time was within 30 s. CONCLUSION: According reasonable prescription and simple product craft, we can product qualified clonazepam oral disintegrating tablets.

Objective To study the effects of direct-current field stimulation on spinal cord motor neurons apoptosis after sciatic nerves transection. Methods In experimental group direct-current stimula tors were put into the relevant spinal cord segments and electric stimulation were given instantly after sciatic nervet ransection.In control group, the same st I mulator were put inside without courrent output.The spinal cord specimens were taken 1，4，7，14，28 days after operation. The morphology of the spinal cord motor neurons was observed with the methods of HE staining and light microscope.The spinal cord motor neurons were counted and analyzed with three-dimensional quantit ative method.The TUNEL staining of the specimens was performed simultaneously and the positive-stain spinal cord motor ne u rons were counted. Results 4，7，1 4 and 28 days af ter operation, the number of spinal cord motor neurons and the size of the neurons increased obviously with compare to the control group.The TUNEL staining positive spinalcord motor neurons decreased sign I ficantly according to the control group. Conclusion Direct-current stimu lation may have intervention effects on spinal cord neuron apoptosis after sciatic nerve transection.