China ; Chronic Disease ; Humans ; Phenotype ; Rhinitis ; genetics ; immunology ; Sinusitis ; genetics ; immunology

Desensitization, Immunologic ; Humans ; Rhinitis, Allergic, Perennial ; therapy ; Rhinitis, Allergic, Seasonal ; therapy

Asthma ; immunology ; Humans ; Rhinitis ; immunology

Allergens ; immunology ; Child ; Desensitization, Immunologic ; Humans ; Rhinitis, Allergic, Perennial ; immunology ; therapy ; Treatment Outcome

Objective　To study the effect of rehabilitation training on the expression of Fos in the central nervous system(CNS) of rats with cerebral infarction. Methods　60 SD rats were made as cerebral infarction models and divided into 2 groups at random 24 hours later: rehabilitation group was given balancing, grasping, rotating and walking and other trainings everyday, while immobiligation group was fixed in cages. Immunohistochemistry was used to detect the Fos expression 1 d, 7 d, 14 d, 21 d and 28 d after infarction respectively. Results　Fos-positive immune reaction appeared in cortex,thalamus, hypothalamus, reticular form and posterior gray horn of the spinal cord of both groups　.Rehabilitation group responded stronger than the immobilization group significantly (P<0.01).Conclusion　Rehabilitation training can activate cortical neurons around the infarted focus and in its opposite side of the CNS, and thus promote the recovery of rats' motor functions.

AIM: Pancreatic and duodenal homeobox gene 1(pdx-1) is a crucial transcription factor in pancreatic islet development and differentiation. This study was conducted to evaluate whether pdx-1 delivered by adeno-associated virus (AAV) could induce liver cells to differentiate into insulin-producing cells in diabetic rats and thus provide more information for cell replacement therapy for diabetes. METHODS: Recombinant AAV vector was employed to deliver pdx-1to STZ-induced diabetic rats via portal vein (4×1011). Blood glucose and body weight were monitored. Gene expression of pdx-1 and insulin were determined by RT-PCR and immunocytotochemistry (ICC) at the 6th week after the injection. RESULTS: AAV-pdx-1 group showed obvious gene expression of pdx-1 and insulin by RT-PCR analysis and the presence of more insulin-positive cells by ICC. Hyperglycemia was partially ameliorated and body weight was also increased in AAV-pdx-1 treated diabetic rats, though still significantly different from those in the non-diabetic group. CONCLUSION: The data indicate that AAV-pdx-1 can induce more rat liver cells into insulin-producing cells in vivo, thereby ameliorate hyperglycemia. Further experiments are needed to explore which subpopulation of liver cells responds to this development shift and the mechanism of this development shift induced by pdx-1 in order to improve the differentiation efficiency.

Objective The study aimed to investigate the effect of lipoxin A4 (LXA4) on lipopolysaccharide (LPS)?induced oxidant stress in human renal tubular epithelial cells (HK2 cells) and possible underlying mecha?nisms. MethodsHK2 cells were divided into three groups: Control ,LPS and LPS+LXA4 groups. After cells were treated with indicated conditions,morphological changes were observed. The expressions of Nrf2 were detected by immunofluorescence and cells were collected for RT?PCR experiments.Results HK2 cells seemed disrupted and necrotic with the administration of LPS. However ,LXA4 could prevent cells from injury induced by LPS. LPS decreased Nrf2 expression and promoted it to translocate to cytoplasm ,while LXA4 could increase its expression and promote it to translocate to nucleus. Moreover ,LPS could decrease Nrf2 and its downstream molecule mRNA expressions,but LXA4 could reverse this effect. Conclusion Our results demonstrated that LXA4 effectively inhibit?ed HK2 cell oxidant stress via Nrf2 pathway.

Female ; Humans ; Infant ; Male ; Ultrasonography ; Urachus ; abnormalities ; diagnostic imaging

3 months) showed that 47. 2% of 36 patients had persistent relief without pain recurrence. Besides pain relief, the interventional endoscopy resulted in weight gain and improvement in quality of life in 63. 9% of 36 patients. Furthermore, the incidences of hyperamylasemia and bleeding which recovered after medication only within 3 days were 13. 9% and 5. 6% , respectively. Stent occlusion and detachment occurred respectively in 12. 5% and 4. 2% of 24 patients undergoing stent placement. Conclusion Endoscopic therapy for patients with pancreatic duct stricture appears to be safe and effective.

Objective To establish a database for models of teeth, which could provide a new technology for personal identification based upon teeth and bite mark for forensic medical practice. Method The (X, Y) digital coordinates were established based up on materials concerning shape and arrangement of the teeth by analyzing samples of 400 models of teeth. The position of every tooth's key point corresponding to the origin was obtained, thus, the characters of tooth and dental arch could be calculated rapidly according to Borland C ++ 5.0 computer language under DOS system. Results The models of teeth could be identified automatically. Among the correlative precision indexes, the length was 1.3mm, the angle was 3?. It seems reasonable to select 12-14 teeth points for studying, which needed about 5-7 complete tooth marks. Conclusion This method is one of the bases in studying personal identification relating to tooth and bite mark.