OBJECTIVE To study the distribution and drug resistance supervision of infection pathogens of Gram-negative bacterial pneumonia in lower respiratory tract of senile patients and draft preventive and control measures.METHODS The sputum and lower respiration secretion of 368 senile patients were cultured to detect pathogens which were confirmed by routine methods.The disk diffusion test was adopted to analyze drug resistantce.RESULTS Pseudomonas aeruginosa rated the top one,reached 25.0%;the drug resistance supervision manifested that the resistant rates of Gram negative organisms to the routine drugs were high except for Haemophilus influenzae.And extensive-spectrum beta-lactamases(ESBLs) occurred with rate of 30.2%.CONCLUSIONS It is very necessary for clinical microbiology department to detect pathogens of lower respiratory tract pneumonia among senile patients and then to give reasonable advice for medicine in time.

OBJECTIVE To investigate the spread status and drug-resistance of pathogenic bacteria causing chronic bacterial prostatitis among the senile patients,and provide the reference for clincal diagnosis and treatment.METHODS Prostatic fluid from the senile patients with chronic bacterial prostatitis was collected,and bacterial cultures and susceptibility tests were performed.The data were analyzed statistically.RESULTS Among 216 prostatic fluid specimens from the senile patients with chronic bacterial prostatitis,140 isolates of pathogenic bacteria were detected.In these strains,Gram-positive cocci accounted for 60.0% and most of them were Staphylococcus aureus.Gram-negative bacilli accounted for 40.0% and most of them were Escherichia coli.Pathogens causing prostatitis have been resistant to the common antibiotics.Among the total staphylococci,meticillin-resistant Staphylococcus(MRS) accounted for 34.8%.And among the total Enterobacteriaceae,ESBLs producing bacteria accounted for 35.6%.CONCLUSIONS Chronic bacterial prostatitis in the senile patients possesses intractability and persisting.It is important to monitor the pathogens and their drug-resistance.

AIM:To develop a method for determining cholic acid by HPLC-ELSD and GC was applied to determing muscone;in Jawei Xihuang Soft Capsule(Calculus Bovis,Moschus,Venenum Bufonis,Olibanum,Myrrha).METHODS:AC_(18) column(Kromasil C_(18),5 μm,4.6 mm×250 mm)was used as stationary phase,the mobile phase was methanol-0.01% glacial acetic acid(73:21)at a flow rate of 1.0 mL/min.The parameters of ELSD were set as follows:evaporation temperature was 40℃,carrier gas(N_2)pressure was 200 kPa.The GC system consisted of DB-1 capillary column(30 m×0.32 mm×0.25 μm)and FID as the detector.The programmed temperature-GC and internal standard method were employed to determine the content of muscone.RESULTS:The linear ranges of cholic acid and muscone were in the range of 45.2 ng-904 ng and 0.05 mg/mL-0.5 mg/mL respectively.The average recoveries were 99.06% and 99.40% with RSD of 1.56% and 0.95% respectively.CONCLUSION:The method is convenient and accurate,and it can be used for the quality evaluation of Jawei Xihuang Soft Capsule.

AIM:To develop a method for determining cholic acid by HPLC-ELSD and GC was applied to determing muscone;in Jawei Xihuang Soft Capsule(Calculus Bovis,Moschus,Venenum Bufonis,Olibanum,Myrrha).METHODS:A C18 column(Kromasil C18,5 ?m,4.6 mm?250 mm)was used as stationary phase,the mobile phase was methanol-0.01% glacial acetic acid(73:27) at a flow rate of 1.0 mL/min.The parameters of ELSD were set as follows:evaporation temperature was 40 ℃,carrier gas(N2) pressure was 200 kPa.The GC system consisted of DB-1 capillary column(30 m?0.32 mm?0.25 ?m) and FID as the detector.The programmed temperature-GC and internal standard method were employed to determine the content of muscone.RESULTS:The linear ranges of cholic acid and muscone were in the range of 45.2 ng-904 ng and 0.05 mg/mL—0.5 mg/mL respectively.The average recoveries were 99.06% and 99.40% with RSD of 1.56% and 0.95% respectively.CONCLUSION:The method is convenient and accurate,and it can be used for the quality evaluation of Jawei Xihuang Soft Capsule.

Anesthesia ; methods ; Brain Ischemia ; prevention & control ; Cerebrovascular Circulation ; Humans ; Intracranial Pressure ; Monitoring, Intraoperative ; Neurosurgical Procedures ; Oxygen ; blood ; Ultrasonography, Doppler, Transcranial

MicroRNAs (miRNAs), a non-encoding small molecular RNA, is entitled to regulate gene expression after transcription, which plays a key role in the progress of tumorigenesis, growth and metastasis. MiR-29 family consists in miR-29a, miR-29b and miR-29c, all featured by the regulation of breast cancer. This review will summarize the regulation and molecular mechanism of miR-29 family in all critical stages of breast cancer, and evaluate the value of miR-29 as a new diagnostic and prognostic biomarker or therapeutic target.

The prognosis of advanced gastric cancer and gastroesophageal junction (GEJ) adenocarcinoma is poor. Although che-motherapy prolongs patient survival and improves quality of life to a greater extent best supportive care compared to, the median over-survival of patients with advanced gastric cancer is limited to approximately 7-10 months. With remarkable progress in the understand-ing of molecular mechanisms, molecular-targeted agents have been developed and evaluated in international randomized phaseⅢclini-cal trials. These agents may change the treatment mode of this disease. A ToGA study initially demonstrated that the trastuzumab, the monoclonal antibody of HER-2, as a molecular-targeted agent, in combination with chemotherapy, can prolong the overall survival of patients to 13.8 months. Several agents targeting angiogenesis, c-Met, PARP, and immunotherapy are currently subjected to clinical tri-als. This review summarizes the current status of molecular-targeted therapies for gastric cancer and GEJ adenocarcinoma.

Clinical trials have demonstrated that arsenic trioxide was effective in the treatment of acute promyelocytic leukemia. Based on this fact, more and more studies have been carried out to study its role in the treatment of human hepatocarcinoma cell. This article reviews arsenic trioxide in trial studies and clinical use of hepatoma carcinoma cell.

Gap junction is one kind of synapse coexisting with chemical synapse in the nervous system. Nowadays with the development of electrophysiological technique,more research began to focus on the role of gap junction in the neural network,especially in the cognitive function. Gap junction collaborates with chemical synapses to induce the neural network activities related to memory and perception,such as gamma and ripple oscillation,which is regarded as the mechanism of the role of gap junction in cognitive function. This review introduces the structure of gap junction,distribution and expression of different kinds of connexins in central nervous system,which are the basic element of gap junction. Furthermore,this review discusses the role of gap junction in cognition related network oscillations and activity-dependent plasticity of gap junction.

OBJECTIVE:To study the stability of the mixture of cefradine for injection and ornidazole sodium chloride injection.METHODS:HPLC method was adopted in which the Nova-pak C 18 was taken as the chromatographic column,methanol-water(35∶65)was taken as the mobile phase with detection wavelength at240nm.The contents were determined after mixing of cefradine for injection and ornidazole sodium chloride injection under the room temperature within8hours,and the appearance of the solution was observed and its pH value was determined.RESULTS:No significant differences were found in terms of the appearance,pH value and the contents of the mixed solution.CONCLUSION:The mixture of ornidazole for injection and cefradine sodium chloride injection can be used under room temperature within8hours of mixing.