In the teaching reform of cytobiology,we should first formulate a reasonable syllabus according to training goals,pay attention to course content reform,strengthen connection with other preclinical medicine subjects,combine quality-oriented education in class instruction,and cultivate students scientific and original spirit.The experimental courses should be offered from higher level and be conformed with subject development.The teacher should have innovative thinking,adopt diversified teaching methods,and fully arouse students' study enthusiasm.

Objective To investigate the relationship between the serum concentration of amiodarone and QT dispersion.Methods The serum concentration of Am and DEA were determined by HPLC during the treatment in forty Patients to study the relationship between the serum concentration and QTd.Results After using amiodarone,QT interval was significantly increased[(383±31)ms vs(418±29)ms,P＜0.05].In the same time QTd was significantly decreased[(34±12)ms vs(21±9)ms,P＜0.05].With maintaining dosage,the serum concentration of DEA had positive relationship with the increment of QT(r=0.50,P＜0.05).While with loading dosage,the serum concentration of amiodarone had no relationship with it.The serum concentration of amiodarone had no relationshiP with the decrement of QTd in all patients.And it varied in a large range.Between the groups of different therapeutic effect,the serum concentration had no statistical difference.Two patients appeared the adverse effects.Their serum concentration of amiodarone weren't higher than the average concentration of the group.Conclusion The serum concentration of amiodarone couldn't be an index tO estimate the effect and the occurrence of adverse effects.

Objective To probe into the application feature and use tendency of traditional Chinese medicine (TCM) injections in our hospital;To evaluate rational drug use and provide reference for related researches. Methods TCM injections in our hospital during 2010-2012 were analyzed by employing the analytic method of defined daily dose, with a purpose to evaluate frequency of drug use and rationality of medication expenses. Results From 2010 to 2012, drug possession rate of TCM injections in our hospital was 4.07%-4.47%;the amount of medication accounted for 10.50%to 12.69%of the amount of all drugs;the annual growth rate increased significantly, among which TCM injections used for removing blood stasis took the lead;frequency of drug use rose annually;medication synchrony in 3 years indicated that daily expensesof Shenkang Injection, Aidi Injection, salvianolateinjection, safflor yellow sodium chloride injection and other TCM injections were on the high side. Conclusion From 2010 to 2012, TCM injections in the clinical application in our hospital were of relatively high frequency, and the TCM injections used for removing blood stasis took the lead. Daily expenses of TCM injections drugs were on the high side and there may be unreasonable application.

A new method of the separation of hydrocarbons and glycerides in liver oil of Mustelus jriseas was designed. The Waters Prep-500A Liquid Chromatograph with silica gel column and differential refractive index detector was used. The mobile phase was petroleum ether and ethyl acetate. The pure hydrocarbons' and glycerides were obtained from the liver oil.

Tripterygium Hypoglaucum Hutch and Tripterygium Wilfordii Hook.f are all Evonymus alata family Tripterygium Wilfordii genus plants.They are generally used in the clinical treatment in department of dermatology.They resemble each other not only in some chemical constitutions,but also in some pharmacologic actions(such as anti-inflammatory,anti-tumor,immune suppression,antifertility and so on),but they are all poisonous plants.When they are used for therapy,they can induce some toxic reactions,even the death of patients.In order to facilitate the adoption of the two drugs,we review their pharmacologic actions.

To study on one case of severe cerebral parlsy. Special education and rehibilitative training for children with severe cerebral parlsy arc investigated in the current study.

Objective: The clinical experience of treatment in 38 patients of Reinke′s edema by surgery of minimally invasive was reported from June 1997 to December 2000. Methods: Thirty-eight patients (25 males and 13 females, ranging in age from 46 to 67 years old) with Reinke′s edema of vocal cord were diagnosed by frequency-flash-laryngoscope and pathologic. The bilateral vocal cord stripping surgery with micro-suspension laryngoscope under general anaesthesia was completed in all 38 patients. Results: After treatment 3 months to 1 year follow-up in 33 cases, 27 cases(81.8%) had acquired good voice and only 6 cases (18.2%) had acquired lower tone voice. Conclusion: None of them had vocal cord adhesive with bilateral vocal cord mucous stripping operation which remained anterior 1/2 parts of vocal cord mucous on one side.

Objective To study portal vein hemodynamics changes after autoiogous bone marrow stem cell transplantation in patients with liver cirrhosis. Methods The hemodynamic parameters, including portal vein diameter, average portal blood flow velocity and spleen size, were determined by Colour Doppler Ultrasonography in 50 patients after autologous bone marrow stem cell transplantation and all cases were followed for up to 6 months. Results (1) After autologous marrow stem cell transplantation, the portal vein diameter reduced significantly at different timepoints compared to before the treatment ([1.41 ± 0. 15] cm, [1. 38 ± 0. 11]cm,[1.36±0. 17] cm vs. [1. 53 ±0. 18] cm,t = 1. 987,1.994,1. 976, Ps < 0.05), but we found no significant difference in the comparisons among different timepoints (F = 2. 35 ,P >0.05). (2) After autologous marrow stem cell transplantation, the average portal blood flow velocity increased significantly at different timepoints compared to before the treatment ([15. 7 ± 3. 6] cm/s, [16. 1 ± 2.4] cm/s, [15. 9 ± 3.0] cm/s vs.[11.4 ± 3. 3] cm/s ,t = 2. 345, 2.460,2. 381, Ps < 0. 01) , but we found no significant differences in the comparisons among different timepoints (F = 2. 14, P > 0.05) . (3) After autologous marrow stem cell transplantation,the spleen size reduced significantly at different timepoints compared to before the treatment ([4.8±0.3]cm,[4.7±0.6]cm,[4.8±0.5]cm vs. [5. 2 ±0. 7]cm,t =2. 289,2. 390,2.425,Ps <0.01), but we found no significant differences in the comparisons among different timepoints (F = 2. 26, P > 0.05) .Conclusion The autologous bone marrow stem cell transplantation can effectively improve the portal vein blood flow,reduce the spleen size,alleviate portal hypertension in patients with liver cirrhosis.

Objective To study the role of bacterial collagenase in wound healing with observing capillary neovascularization, epidermis regeneration and collagen fiber reparation in vivo. Method A total of 28 New Zealand rabbits were made into models of superficial soft tissue trauma and divided into two groups. In the experimental group, the right limbs of rabbits were traumatized intentionally in the same length and depth, and their wounds were treated with bacterial collagenase liquor 133 MIU/mL, and the wounds were sutured. Similarly, in the control group, the left limbs of the rabbits were modeled in the same way and their wounds were managed with physiological saline instead and closed. Their wounds were observed continually 1, 2, 3, 5, 7, 10 and 14 days after traumatized. Data were analyzed statistically by using One-way ANOVA, LSD-test, and Wilcoxon rank test. Results There were no significant differences in capillary neovascularization and epidermis regeneration between two groups ( F = 0.12, P = 0.740 and F = 0.95, P = 0.33, respectively), but significant difference in collagen fiber reparation was found between two groups (F = 6.63, P = 0.01). Conclusions In the progress of wound healing, bacterial collagenase can improve the collagen fiber reparation without effects on the new capillary vasculogenesis and epidermis regeneration. The bacterial collagenase does not play a positive role in wound healing.

As we know more and more about asthma pathogenesis and chronic inflammatory disorder mechanism,and additional immunomodulator options become available.Studies have suggested that Th2 cell differentiation increases,and Th1 cell differentiation reduces in asthma.Therefore,it becomes an important immunotherapy method to modulate the Th1/Th2 response by promoting Th1 cytokines and inhibiting Th2 cytokines.At present,the use of immunomodulator such as anti-IgE antibody,anti-IL-5 agents,CpG oligodeoxynucleotides,imiquimod,bacterial extracts,Chinese medicine in asthma has already made some progress,and besidse it demonstrate great efficacy in clinical.