This article introduces four cases of children moyamoya disease,female central precocious puberty,children restless legs syndrome and damsel retrograde menstruation,which were treated by Yang Shaoshan.Doctor Yang insists that the pediatric difficult diseases should be treated with the methods of nourishing yin,nourishing kidney and lower evil fire and clearing and nourishing the Spleen and Stomach,of which protecting yin essence is therapeutic core.

This article explored professor YANG Shao-shan's clinical experiences in treating senile diseases with ointment.He proposes that the TCM method is as following: First,invigorating kidney for protecting semen,pay attention to nourishing yin and decreasing yang.Second,invigorating spleen and stomach,pay attention to "vegetarian diet".Third,eliminating pathogenic factor,like phlegm and stasis.Forth,reinforcing the three treasures.

OBJECTIVE: To establish an HPLC method to determine the content of Baicalin in Oral liquid for gastritis. METHODS: Zorbax SB-C18(150 mm?4.6 mm,5 ?m) was used as chromatographic column, and the mobile phrase consisted of methanol-water-phosphoric acid(50∶50∶0.2) with detection wavelength set at 276 nm.RESULTS: The calibration curve of baicalin was in good linearity over the range of 0.065～0.520 ?g (r=0.999 8). The average recovery of Baicalin was 99.79% with RSD at 1.28% (n=6).CONCLUSION: The method is simple and accurate and applicable for the quality control of Oral liquid for gastritis.

Ethyl pyruvate ( EP) is a stable lipophilic ester derivative of pyruvate with many pharmacological actions confirmed by researches , the anti-tumor effects of EP has attracted the attention of many domestic and foreign scholars .EP exerts antitumor activi-ty through several ways , such as inhibiting proliferation , inducing apoptosis , inhibiting angiogenesis , blocking cell cycle .This paper briefly reviews the rencent findings of antitumor function of EP .

There is a close relationship between changes in endocrine function and related diseases.Thyroid and breast both belong to hormone dependent organs.There are some common pathogenic risk factors between thyroid diseases and breast cancer.Most of the patients with breast cancer are complicated by thyroid morphologic changes,pathogenic changes and functional changes.Although the researches on the relationship between the thyroid diseases and breast cancer have made some progresses,the results are less consistent and the specific mechanism is remain unclear.

Objective To observe the selective regulation of peroxisome proliferator-activated receptors (PPAR) on fatty acid binding protein-4 (FABP4) in human syncytiotrophoblasts.Methods Cultivate normal human syncytiotrophoblast cells,and put in the specific antagonists and agonists of PPAR each subtypes receptors,then observe the different expression of FABP4 mRNA and protein.Results Pretreated the human syncytiotrophoblast cells with the agonists (GW7647,GW0742) and antagonists (GW6471,GSK0660) of PPARα and PPARβ receptors,the expression of the FABP4 was not significantly change (P＞0.05).However pretreated with PPARγ agonists (rosiglitazone,1 × 10-9,1 × 10-8,1 × 10-7 and 1 × 10-6 mol/L),the expression of FABP4 mRNA and protein could be dose dependent-promoted significantly (mRNA:1.27 ±0.12,1.45 ±0.14,1.57±0.14,1.72 ±0.12,protein:1.10 ±0.08,1.37 ±0.09,1.60 ±0.13,1.79 ± 0.14 ; P ＜ 0.05),furthermore,the promotion can be dose dependent-reversed by specific antagonists GW9662 (mRNA:0.92 ± 0.06,0.77 ± 0.06,0.64 ± 0.05,0.55 ± 0.05,protein:0.91 ±0.03,0.78 ±0.06,0.70±0.07,0.55 ±0.06; P ＜ 0.05).Conclusions In normal human syncytiotrophoblast cells,FABP4 is a target factor of PPARγ.PPARγ regulated the expression of FABP4 mRNA and protein selectively.And the regulation will not be influenced by the other two PPAR subtypes.

Objective To investigate the detection of malnutrition in patients with advanced cancer indicators for accuracy . Methods From January to September in 2014 ,100 patients with advanced Ⅲ‐Ⅳ stage cancer were treated in Hangzhou cancer hos‐pital .The clinical data of these patients were retrospectively reviewed ,the data including albumin ,hemoglobin ,total protein ,fasting blood‐glucose ,lactate dehydrogenase ,cholesterol ,triglycerides ,blood urea nitrogen and creatinine and other indicators .Patients′nu‐tritional status was evaluated by PG‐SGA scale and according to the result was graded into PG‐SGA grade A(n=70) ,B(n=8) and C(n=22) .Patients were divided into good nutrition ,moderate malnutrition(potential potentially malnutrition) ,severe malnutrition groups .Take PG‐SGA score as a standard ,evaluation of the above‐mentioned nutritional status of patients with hematology detec‐tion index evaluation accuracy .Results There were significant differences in patients with advanced malignant tumor in hemoglobin and serum albumin among PG‐SGA grade A ,B and C(P<0 .05) .Hemoglobin showed difference between patients of PG‐SGA grade A and C(P=0 .000) ,grade B and C(P=0 .025) .Albumin showed difference in patients between grade A and B(P=0 .003) ,grade A and C(P=0 .000) .Conclusion Hemoglobin ,and serum albumin can be used for evaluating nutritional status in patients with ad‐vanced cancer .

Objective To establish animal model of guinea pig anaphylactic shock ,explore the expression of IL‐4 and IL‐10 in guinea pig serum ,provide scientific and objective evidence for medicolegal and clinical diagnosis of anaphylactic shock as well .Meth‐ods After 40 guinea pigs were classified into the experimental and the control groups ,in which the experimental group was 30 and the control group was 10 ,Elisa method was used to detect IL‐4 and IL‐10 by extracting serum of guinea pigs in the experiment group and the control group .Results The IL‐4 levels of serum in experimental group was higher than that in control group (P<0 .01);the IL‐10 levels of serum in experimental group was higher than that in control group (P<0 .01) .Conclusion The level of serum IL‐4 and IL‐10 in the experiment group are obviously higher than that of the control group .This provides an evidence for an‐aphylactic shock forensic identification and clinical diagnosis .

An article entitled "Adrenal incidentalomas are tied to increased risk of diabetes: findings from a prospective study" published in JCEM in April 2020, was hereby translated into Chinese after obtaining the copyright [Giuseppe R, et al. J Clin Endocrinol Metab, 2020, 105(4): dgz284]. This is a prospective study to explore the frequency of adrenal incidentalomas and their association with comorbid diseases. It recruited 601 patients who underwent abdominal CT scan in the radiology department at a public hospital. Those with any history or doubt of adrenal diseases or malignancy were considered as non-eligible. In 7.3% of the patients, adrenal incidentalomas were serendipitously found. The patients with an adrenal incidentaloma were with higher body mass index( P=0.009) and higher waist circumference( P=0.007) and were more frequently diabetic(31.8% vs 14.2%, P=0.003 8). Multivariate regression analysis showed that diabetes was significantly associated with the presence of adrenal incidentalomas( P=0.003). Autonomous cortisol secretion was observed in 50% of patients(plasma cortisol≥50 nmol/L after 1 mg dexamethasone). This is the first study showing that the frequency of adrenal incidentalomas is 7.3% in a prospective radiological series avoiding ascertainment bias and adrenal incidentalomas are tied to increased risk of type 2 diabetes.

Objective: To study the inhibitory and apoptosis-inducing effect of vitamin E succinate (VES) on androgen-independent prostate cancer cell line PC-3 in vitro. Methods: VES was dissolved with ethanol to obtain VES solution. PC-3 cells of logarithmic growth phase were treated with various concentrations of VES solution (25, 50, 75, 100, and 125mg/L); cells in control group were treated with 1.25% ethanol. MTT method was used to measure the viability and inhibitory rate of cells in each group 24h, 48h and 72h after VES treatment; flow cytometry was employed to determine the apoptosis rate of the PC-3 cells. Results: The viability of cells in the experimental groups was significantly lower than that in the control group (P<0.05). The viability of cells in the experimental groups was negatively correlated with the concentration and exposure period of VES solution; the viability of cells in the control group was positively correlated with the exposure period of VES solution. The apoptosis rate of cells in the experimental groups was much higher than that in the control group; the rate in the experimental group was positively correlated with the concentration and exposure period of VES solution. The optimal induction of apoptosis was achieved af ter 48 h exposure to 75 mg/L VES solution, with a apoptosis rate above 80%. Conclusion: VES can inhibit the proliferation of PC-3 cells and can induce apoptosis of them, which casts new lights on prevention and treatment of prostate cancer.