With glucose as substrate, sodium tripolyphosphate as the phosphorus acylating agent, and phosphorylase of Solanum tuberosum as the catalyst, glucose 1-phosphate was synthesized. Based on a three-level, three-variable Box-Behnken experimental design, response surface methodology was used to evaluate the effects of temperature, molar ratio of glucose to sodium tripolyphosphate and time on the production. The structure of the product was confirmed by 1H NMR spectra. The results show that the optimum conditions were as follows: temperature 35 degrees C, molar ratio of glucose to sodium tripolyphosphate 1.35:1 and time 19 h.
Catalysis ; Glucose ; metabolism ; Glucosephosphates ; biosynthesis ; Phosphorylases ; metabolism ; Polyphosphates ; chemistry ; Solanum tuberosum ; enzymology ; Surface Properties

Red doctor's culture shouldering the important mission of inheriting the red gene and the promotion of medical and health culture.As a multi-dimensional concept of cross-discipline and cross-domain,the cultural momentum space is a mechanical space for the dynamic development of culture.In the context of new era culture and new mission,taking red doctor's culture as a mechanical space field,the paper analyzed the essential connotation of"why red doctor's culture is new in the new era".From the three vectors of diachrony,synchrony,and immediacy,this paper argued for the value implications of"why red doctor's culture needs to be new in the new era".The methodology of"how to promote the new of red doctor's culture in the new era"was formed based on the integration and coherence of excellent medical and health cultures from ancient and modern times,both at home and abroad,combined with the actual laws and necessary rhythms of red medicine culture to push forward,used the internal and external driving forces to help the effective renewal of the space energy of the red doctor's culture in the new era.