1.Feasibility of serum galactomannan assay in hematologic malignancy patients with invasive fungal infections
Journal of Leukemia & Lymphoma 2010;19(7):427-431
Objective To evaluate the feasibility of the serum galactomannan (GM) assay as a rapid detection method for the early diagnosis of invasive fungal infections (IFI) in haematological malignancy patients. Methods Thirty-nine patients with haematological malignancies at high risk of IFI were enrolled into this study. The criteria of high risk included fever (≥ 38℃) lasted for more than 96 hours, fever didn' t response to proper broad spectrum antibiotic or recurrenced after short period of response, and no antifungal drug was used in the last week. The GM assay in serum specimens were performed twice a week for 3 weeks. Thirty health donor were selected as control group to perform the serum GM assay. The sensitivity and specificity, the positive and negative predictive value (PV+, PV-) of GM assay in serum specimens were calculated and compared with traditional diagnosis methods. Results In the 39 patients, 31 patients were diagnosed as IFI by clinical evidence and the other 8 patients were diagnosed as bacteria infection. The cut-off of GM assay was 0.5. GM assay results showed that the positive rate, sensitivity, specificity, total consistent rate, PV+ and PV- were 80.6 %, 87.1%, 62.5%, 82.0%, 90.0 % and 55.6%, respectively. The Kappa rate was 0.474. In the 8 patients without IFI, 3 cases were GM positive and 2 BG positive. The time of the first positive GM assay was (2.8±4.8) d (ranged from 24 d before to 3 d after clinical diagnosis) before IFI was diagnosed. During antifungal treatment, the level of GM maintained highly in the patients with aggravation of IFI, and dropped with the IFI improving. Conclusion The results of GM assay were consistent with that of traditional IFI diagnosis. Compared with classical IFI diagnosis, the GM assay has the advantages of the early, rapid, and high sensitivity and specificity.
2.The development of one kind of safety protection device for lead cable used in equipment and its application in clinical practice
China Medical Equipment 2017;14(6):12-14
Objective:To develop a safety protection device for lead cable of equipment in order to reduce or avoid the skin burn of patient and the invalidation of monitoring data caused by the short circuited of lead cable, and enhance the comfortable level of patient, at the same time, and reduce the pain of patient and potential adverse events.Methods: The device was consisted of the fixed sleeve of cylinder-shape (protective shell) and fixed sleeve of packaged cable (fixed sleeve of wire and cable), and its component was carbon fiber reinforced polycarbonate and silicone rubber. The fragile, repaired part or the rupture rubber of shell in upside and bottom of device were clamped in fixed sleeve. And then, the upside and bottom were fixed in one device, and take them socket with lead cable of device.Results: After the safety protection device of leading cable were used, the cable were fixed in the interior of the device, and could be protected. Therefore, some risks of adverse event were reduced and avoided.Conclusion: The device is stable in performance, is simple and convenient in operation and installation. In the application, it avoids the risk which caused by short circuit of cable in therapy monitoring, and could enhance the comfortable feel. This device is appropriate to various installation and application of lead cable, and it can be repeated to apply. It has got national patent for utility models due to its favourable application value.
3.Features analysis of hidden blood loss in the elderly femoral intertrochanteric fractures treated with extramedullary and intramedullary fixations or hip arthroplasty
Baoshan LI ; Ling LENG ; Cheng LI
The Journal of Practical Medicine 2015;(18):3004-3007
Objective To analyze the features of hidden blood loss in the elderly femoral intertrochanteric fractures treated with extramedullary dynamic hip screw (DHS), intramedullary nails (Gamma 3, PFN) and hip arthroplasty (LBFH). Methods The clinicle records of 193 elderly patients (ages ≥75 year old) with femoral intertrochanteric fractures treat by DHS,Gamma3, PFNA and LBFH in our hospital were retrospectively analyzed. The estimated blood loss were calculated by Gross equation, according to the height,weight and changes of blood routine test reoperative and postoperative,the differences of hidden blood loss among DHS group,Gamma 3 group, PFN group and LBFH group were compared. Results Total blood loss in intramedullary nail groups were significantly higher than DHS group [(766 ± 83) mL],P < 0.05). No significant difference was found between PFN group [(887 ± 75) mL] and Gamma 3 group [(903 ± 91) mL] (P > 0.05), The hidden blood loss were significantly higher in the intramedullary nail groups than those in the LBFH group [(453 ± 98) mL,accounting 54%] and DHS group [(429 ± 59) mL,accounting 56%] (P < 0.05). No significant difference of hidden blood loss was found between Gamma 3 group [(742 ± 137) mL, accounting 82%] and PFN group [(711 ± 153) mL,accounting 80%], (P > 0.05). Conclusion Noteworthy, the hidden blood loss is major part of perioperative total blood loss in the elderly femoral intertrochanteric fractures , intramedullary fixations may result in greater hidden blood loss than extramedullary fixations and hip arthroplasty ,which may cause postoperative anemia.
4.ENDOMORPHIN-1 IS MORE POTENT THAN ENDOMORPHIN-2 IN INHIBITION OF SYNAPTIC TRANSMISSION IN SUBSTANTIA GELATINOSA OF ADULT RAT SPINAL CORD
Dongni LENG ; Yupeng FENG ; Yunqing LI
Chinese Journal of Neuroanatomy 2005;21(3):269-275
Effects of endomorphin-1 (EM-1) and endomorphin-2 (EM-2) on synaptic transmission were investigated on neurons in substantia gelatinosa (SG) of the spinal dorsal horn by whole-cell voltage clamp recording. Both EM-1 (1 μmol/L) and EM-2 (1 μmol/L)remarkably reduced the frequency but not the amplitude of miniature excitatory postsynaptic currents (mEPSCs) and miniature inhibitory postsynaptic currents (mIPSCs). These effects were antagonized by 3-funaltrexamine ( β-FNA, 10 μmol/L), a selective μ-opioid receptor antagonist. Noticeably, EM-1 showed higher potency in decreasing the frequency of mEPSCs and mIPSCs than that of EM-2. These results indicate that EMs suppress both excitatory and inhibitory synaptic transmission by activating presynaptic μ-opioid receptors in the SG and EM-1, compared with EM-2, might be a more potent endogenous analgesic at the spinal cord level.
5.The comparison between Chinese and American medical education and its inspiration
Shouqiang LI ; Yue LIU ; Xiaoping LENG
Chinese Journal of Medical Education Research 2015;(1):14-16
The medical education of China and the United States was compared by analyzing the differences about educational system, teaching mode and teaching content in this paper. Standard-ization of the Chinese medical education system, improving clinical practice, teaching students by initi-ating questions and advanced medical education technology were recommended in China. This paper will inspirit and provide experience for the innovation of Chinese medical education.
6.Induction of immunotolerance in mouse pancreatic islet transplantation through PD-1/PD-L1 costimulatory pathway
Tao LI ; Jiye ZHU ; Xisheng LENG
Chinese Journal of General Surgery 2009;24(1):45-48
Objective To investigate the role of recombinant adenovirus Ad-PD-L1 on immunotolerance induction in mouse pancreatic islet transplantation. Methods Full-length mouse PD-L1 cDNA linked with an internal ribosome entry site (IRES)-GFP cassette was subcloned into pShuttle-GFP-CMV( - ) shuttle plasmid. The product was cut by certain restriction endonuclease and ligate with pAdxsi vector. The adenovirus bone plasmid was transformed into DH5α competent bacteria. The positive clone was identified by restriction endonuclease digestion and further confirmed by sequencing. After linearization, the recombined adenovirus DNA was transfected into 293 cells by liposome for package and amplification, which was purified by CsC1 density gradient centrifugation. Streptozocin was injected i.p. into C57BL/6 (H-2b) mouse to induce diabetic model recipient. Recipients were randomly divided into three groups, Group A was the control. Group B and group C were injected of Ad-EGFP and Ad-PD-L1 through tail vein respectively 1 day before islet transplantation. 300 to 400 islets of DBA/2 (H-2d) were transplanted into the renal subcapsular space of the diabetic model recipient. The level of blood sugar and the graft survival time were monitored. Results Recombinant adenovirns Ad-PD-L1 have high efficiency expression of PD-L1 in recipient mouse. The survival time of grafts of Ad-PD-L1 group (27.63 ± 3. 51 ) d was significantly longer than that of the control ( 7. 85 ± 0. 33 ) d and Ad-EGFP group ( 7. 67 ± 0. 59 ) d ( P < 0. 01 ). Mixed lymphocyte response showed a specific decrease reaction of recipient lymphocyte toward donor lymphocytes. Conclusion Recombinant adenovirus Ad-PD-L1 was successfully constructed. In mouse pancreatic islet transplantation, it can suppresses the activation of recipient T lymphocyte through PD-1/PD-L1 co-stimulatory pathway, and significantly prolong the survival time of grafts.
7.The Application of Fat-suppressed Spoiled Gradient-Echo (3D-FS-SPGR) Sequence in Association withThree-Dimensional Reconstruction in Cartilage Disorder of Knee
Sirun LIU ; Xiaoming LENG ; Li HUANG
Journal of Practical Radiology 1992;0(11):-
Objective To evaluate the clinical value of 3D-FS-SPGR and 3D-reconstructional technique in detecting the lesion of articular cartilage of knee.Methods Fifty eight knees with OA(42 cases)or trauma (9 cases) in 54 cases (12 cases were confirmed by operation) were imaged with eight different MR imaging sequences including T 1-weighted,proton density-weighted,T 2-weighted FSE,STIR,3D-FS-SPGR,2D-FS-SPGR,3D-SPGR and 3D-FS-GRE.The classification and three-dimensional reconstruction of cartilage in four locations of knee were performed on different MR sequences.The detecting rate of cartilage lesion were calculated.The results in partly cases were analysed compared to operation. Results The detecting rate was different on each sequence as follow:T 1WI,GRE,STIR,PDWI,T 2WI and SPGR were 8.91%,16.36%,19.32%,19.57%,21.36% and 24.09% respectively (? 2=23.523,?
8.Investigation of Residual Chlorine Contents in Hospital Wastewater of Jinan Urban Areas
Peiqing CHENG ; Jiafeng LENG ; Dong LI
Journal of Environment and Health 1992;0(04):-
Objective To understand the contents of residual chlorine in the wastewater of 12 hospitals in the urban districts of Jinan. Methods Wastewater samples discharged from 12 hospitals were collected from June to September in 2003. The contents of residual chlorine in the wastewater samples were determined using iodometric titration method and the fecal coliform counts of the wastewater were determined. Results The contents of residual chlorine in the wastewater of 12 hospitals were 3.7 mg/L-128 mg/L with the average level of 27.6 mg/L. The result of the fecal coliform count in the wastewater was accorded with the National Hygienic Standard. Conclusion The contents of residual chlorine in the wastewater discharged from municipal hospitals in Jinan were too high, which might cause the secondary pollution to water environment.
9.Management of biliary complications after orthotopic liver transplantation
Jiye ZHU ; Guangming LI ; Xisheng LENG
Chinese Journal of General Surgery 1997;0(06):-
Objective To study the causes and management of biliary complications after orthotopic liver transplantation.MethodsClinical data of 38 patients who have underwent orthotopic liver transplantation between May 2000 and January 2002 were studied retrospectively. ResultsPostoperative bililary complications occurred in 9 patients(24%), including simple biliary fistula in 4, stricture of hepatoenterostomy, intrahepatic cholelith, biliary stricture with sludge, biliary stricture with cholelith, and intrahepatic biloma and cholelith in one each. Two patients died of the complication, and seven were cured successfully. ConclusionsThe causes of biliary complications are complicated. Early diagnosis and timely management helps to decrease the mortality. Endoscopic retrograde cholangiopancreatography and percutaneous transhepatic cholangiography is a valuable, less traumatic procedure.
10.The expression and prognostic implication of p27~kip1 in patients with stage Ⅰ and Ⅱ colorectal carcinoma
Jiahun LENG ; Jin GU ; Jiyou LI
Chinese Journal of General Surgery 1997;0(04):-
Objective To investigate the expression and prognostic significance of p27kip1 in patients with stage I and Ⅱ colorectal carcinoma ( CRC). Methods We retrospectively analyzed the expression of p27kip1 by immunohistochemistry in paraffin-embedded specimens from 114 consecutive patients operated for stage I and Ⅱ CRC. Statistical analysis was done with SPSS10. 0 package. Results The rate of p27kip1 over-expression in stage I and ⅡCRC was 85. 1 % (97/114). The expression of p27kip1 was statistically associated with TNM stage and histologic grade, while there was no significant relationship between p27kip1 expression and patient's age, tumor location. The 5-year disease free survival rate of the patients with p27kip1 over-expression was significantly higher than those with p27kip1 low-expression (88. 4% vs. 52. 9% , P