1.Physiological roles of the receptors for purines and pyrimidines
Chinese Pharmacological Bulletin 1987;0(01):-
Purines and pyrimidines have widespread and specific action in many tissues of both invertebrates and vertebrates. These compounds have powerful physiological roles in both short-term actions of neurotransmission, exocrine and endocrine secretion, and regulation of immune cell function,and long-term actions of cell growth, cell differentiation and proliferation in the development and regeneration.
2.Effects of adenosine A_1 receptor antagonist on the hippocampal BDNF protein expression and endoplasmic reticulum content of rats
Danshen ZHANG ; Leiming REN ; Li ZHANG
Chinese Pharmacological Bulletin 1987;0(02):-
Aim To study the effect of blocking adenosine A_1 receptors on BDNF protein expression and the endoplasmic reticulum(ER) content of the rat hippocampus.Methods The effect of DPCPX on BDNF protein expression in granular cells of the rat hippocampus was observed using immunohistochemical method,and the effect of DPCPX on ER in neurons of the rat hippocampus was observed with transmission electron microscope.Results DPCPX(0.1 mg?kg~(-1),ip,15 d) significantly increased the number of BDNF positive cells in hippocampus granular cells.DPCPX(0.5 mg?kg~(-1),ip,15 d) significantly increased both the levels of BDNF protein and the number of BDNF positive cells in hippocampus granular cells(P
3.Diclofenac sodium extended-release capsule for cancer pain in senile patients
Yong ZHANG ; Leiming WENG ; Jie SHEN ;
China Oncology 2001;0(05):-
Purpose:To study analgesia effect and adverse reaction of diclofenac sodium extended release capsule for cancer pain in senile patients. Methods:20 senile patients with light or moderate cancer pain were selected.The analgesia effect and adverse reaction were observed after diclofenac sodium extended release capsule 100 mg qn po.Results:The treatment of cancer pain with diclofenac sodium extended release capsule in senile patients had obvious analgesia effect without obvious adverse reaction. Conclusions:Diclofenac sodium extended release capsule 100 mg qn po is a safe,effective and simple way to relieve cancer pain in senile patients with light or moderate cancer pain.
4.Determination of Salvianolic Acid B in Qianlie Kangxin Granules by HPLC
Fengqin ZHANG ; Wei GONG ; Leiming QU
China Pharmacy 2007;0(33):-
OBJECTIVE: To develop an HPLC method for the determination of salvianolic acid B in Qianlie kangxin granules.METHODS: The sample was determined on DiamonsilTM C18(250 mm?4.6 mm,5 ?m) with the mobile phase consisted of methanol-acetonitrile-water-formic acid(25∶10∶64∶1) at a flow rate of 1.0 mL?min-1.The detection wavelength was set at 286 nm.RESULTS:The linear range of salvianolic acid B was 0.206~1.854 ?g(r=0.999 9).The average recovery rate for salvianolic acid B was 98.0%(RSD=1.5%,n=6).CONCLUSION: The established method is simple,reliable,stable in results and reproducible,and it is applicable for the quality control of Qianlie kangxin granules.
5.Studies on antiarrhythmic portion in Cornus officinalis extract
Lantong ZHANG ; Leiming REN ; Jinkun WEN ;
Chinese Traditional and Herbal Drugs 1994;0(11):-
Object To study the effective portion in Cornus officinalis Sieb. et Zucc. extract (COE) for the treatment of arrhythmia. Methods Effect of COE on chloroform induced ventricular fibrillation in mice and electrophysiology of isolated guinea pig papillary muscle were studied. Results Antiarrhythmic effect of COE may be related to its prolongation of action potential duration, increase of the absolute value of resting potential and a decrease of autonomy of sinus node. The effective portion in COE may be its total organic acid and a certain yet unknown trace substance, whereas its total glycosides were devoid of such activities. Conclusion Pharmacodynamic and myocardial electrophysiologic studies showed that the total organic acid and a certain unknown trace substance possessed the obvious antiarrhythmic activity.
6.Combined administration of rhubarb and niatrii sulfas for severe acute pancreatitis
Jun LI ; Hui ZHANG ; Leiming ZHU ; Xuezhi WANG ; Junling DING
Chinese Journal of General Surgery 1997;0(06):-
Objective To discuss the therapeutic effects of rhubarb and natrii sulfas for severe acute(pancreatitis)(SAP).Methods Eighty-three patietns with SAP were randomly divided into treatment group(group A,n=45) and control group(group B,n=38).The 2 groups received the same therapy except that group A received rhubarb and natrii sulfas through nasogastric tube.Results In treatment group(compared) to control group,the clinical symptoms such as abdominal pain and distention were more quickly(relieved),with early return to oral food intake,lesser complications and shorter hospital stay(all with P
7.Effects of curcumin combining with adriamycin to kill KB and KB_(v200) cells
Huizhu ZHANG ; Lin YANG ; Leiming REN ; Shumei LIU ;
Chinese Pharmacological Bulletin 1986;0(06):-
AIM To investigate antitumor effects of curcumin (Cur) combining with adriamycin (ADR) on human tumor cell lines in vitro and study the mechanisms. METHODS The antitumor effects of the drugs on tumor cell lines in vitro were determined with MTT method. The Jin's formula was used to analyze the effect of drug combination. Cellular ADR accumulation was measured by fluorescence spectraphotometry. RESULTS In simultaneous adiministration Cur 4 25~34 00 ?mol?L -1 combining with ADR produced a simple addition or potentiation effect. The cellular ADR accumulation was markedly increased in the presence of Cur in MDR cells, but decreased in sensitived cells. The experiment of membrane fluidity show that that Cur affects membrane fluidity neither on KB nor on KB v200 cell line. CONCLUSION Simultaneous adiministration of Cur combining with ADR produces synergistic effect. The mechanism is connected with increasing cellular ADR accumulationon on KB v200 cell line. While on KB cell line, it is related to synergistic pharmacological effect. But the action of Cur is not connected with the change of membrane fluidity on both cell lines.
8.Factors related with difficulty of LC in acute calculus cholecystitis
Jun LI ; Hui ZHANG ; Leiming ZHU ; Junling DING
Chinese Journal of General Surgery 2001;0(08):-
Objective To explore the factors related with difficulty of laparoscopic cholecystectomy(LC) for acute calculus cholecystitis.Methods A total of 245 patients were retrospectively divided into two groups:easy group(123patients)and difficult group(122patients,including 32patients of conversion to OC)based on surgery time and LC or OC.Results There were statistical differences between the groups in regards to preoperative body temperature,white blood cell count,surgical timing,the thickness of gallbladder wall,gallbladder volume,and diameter of common bile duct(all P
9.Effects of integrin-targeted photodynamic therapy on pancreatic carcinoma cell
Qianwen NI ; Shanying YANG ; Chunying QU ; Min ZHOU ; Pengcheng ZHAO ; Jiancheng ZHANG ; Leiming XU
Chinese Journal of Digestion 2013;33(4):253-258
Objective To investigate the anti carcinoma role of integrin targeted photodynamic therapy (PDT) on pancreatic carcinoma cells in vitro.Methods Pancreatic carcinoma cells SW1990 were divided into four groups:cells without quantum dots (QDs) and light-treated as blank control group,pure light-treated group,photosensitizer group and PDT group.The targeting of QDs-arginine,glycine,aspartic acid (RGD) and integrin probe was confirmed by laser confocal microscopy.And as a photosensitizer for photodynamic therapy,after treated for 48 hours the morphology changes of pancreatic carcinoma cells of each group were observed.After 48 hours,the cell proliferation,apoptosis and cell cycle changes were detected by methyl thiazolyl tetrazolium (MTT) assay and flow cytometry (FCM).The expressions of myeloid cell leukemia-1 (Mcl-1),protein kinase B(Akt) and tumor necrosis factor-related apoptosis inducing ligand (TRAIL) were detected by reverse transcriptase polymerase chain reaction(RT-PCR).The amount of reactive oxygen species (ROS) of each group were evaluated by fluorescence probe.One-way ANOVA was performed for comparison between groups to analyze the treatment effects of PDT group.Results The QDs RGD probe could effectively targeting pancreatic carcinoma cells.The MTT results indicated that the relative inhibition rate of pancreatic carcinoma cells proliferation of PDT group was statistically higher than that of the other groups at 24,48,72 h (F=73.00,85.10,126.58; all P<0.01).The FCM results revealed that the cell apoptosis rate of PDT group (17.860% ±1.230%) was higher than that of the other groups (F=130.617,P<0.01) and cell cycle G0/G1 phase (69.14%±2.63%) and S phase (24.41% ± 2.67 %) retardance was also significant (all P<0.05).The expression of proliferation and apoptosis related gene Mcl-1 and Akt at mRNA level was lower than that of the other groups however the expression of apoptosis-inducing ligand TRAIL at mRNA level was higher than that of the other groups (F=567.456,446.817,145.238; all P<0.05).The ROS level of PDT group was higher than that of the other groups (F=3262.559,P<0.01).Conclusion PDT with a QDs-RGD probe could significantly inhibit pancreatic carcinoma cell proliferation and promote cell apoptosis.
10.Application of rotary biopsy forceps in diagnosis of gastric pre-cancerous lesions
Min ZHOU ; Leiming XU ; Chunying QU ; Yi ZHANG ; Wenchao WU ; Ying CHEN ; Huifang CHEN
Chinese Journal of Digestive Endoscopy 2010;27(2):64-66
Objective To evaluate the sampling efficiency of rotary biopsy forceps in gastric precancerous lesions. Methods A total of 60 gastric lesions with suspected pre-cancerous characters under narrow band imaging were enrolled,and consecutive samples were taken from same lesion by one endoscopist with routine and rotary forceps,respectively. The most severe pathological diagnosis was regarded as the final diagnosis. Results There was a significant difference between rotary biopsy forceps and routine ones in regarding of sample quality and capability of minimize tissue damage (P<0. 05). The concordance rate with final pathological diagnosis from sample taken by rotary biopsy forceps was higher than that from routine ones,but without significance (P>0. 05). Conclusion The rotary biopsy forceps is superior to routine ones in sampling of gastric pre-cancerous lesions.