Purines and pyrimidines have widespread and specific action in many tissues of both invertebrates and vertebrates. These compounds have powerful physiological roles in both short-term actions of neurotransmission, exocrine and endocrine secretion, and regulation of immune cell function,and long-term actions of cell growth, cell differentiation and proliferation in the development and regeneration.

Aim To study the effect of blocking adenosine A_1 receptors on BDNF protein expression and the endoplasmic reticulum(ER) content of the rat hippocampus.Methods The effect of DPCPX on BDNF protein expression in granular cells of the rat hippocampus was observed using immunohistochemical method,and the effect of DPCPX on ER in neurons of the rat hippocampus was observed with transmission electron microscope.Results DPCPX(0.1 mg?kg~(-1),ip,15 d) significantly increased the number of BDNF positive cells in hippocampus granular cells.DPCPX(0.5 mg?kg~(-1),ip,15 d) significantly increased both the levels of BDNF protein and the number of BDNF positive cells in hippocampus granular cells(P

Purpose:To study analgesia effect and adverse reaction of diclofenac sodium extended release capsule for cancer pain in senile patients. Methods:20 senile patients with light or moderate cancer pain were selected.The analgesia effect and adverse reaction were observed after diclofenac sodium extended release capsule 100 mg qn po.Results:The treatment of cancer pain with diclofenac sodium extended release capsule in senile patients had obvious analgesia effect without obvious adverse reaction. Conclusions:Diclofenac sodium extended release capsule 100 mg qn po is a safe,effective and simple way to relieve cancer pain in senile patients with light or moderate cancer pain.

Object To study the effective portion in Cornus officinalis Sieb. et Zucc. extract (COE) for the treatment of arrhythmia. Methods Effect of COE on chloroform induced ventricular fibrillation in mice and electrophysiology of isolated guinea pig papillary muscle were studied. Results Antiarrhythmic effect of COE may be related to its prolongation of action potential duration, increase of the absolute value of resting potential and a decrease of autonomy of sinus node. The effective portion in COE may be its total organic acid and a certain yet unknown trace substance, whereas its total glycosides were devoid of such activities. Conclusion Pharmacodynamic and myocardial electrophysiologic studies showed that the total organic acid and a certain unknown trace substance possessed the obvious antiarrhythmic activity.

OBJECTIVE: To develop an HPLC method for the determination of salvianolic acid B in Qianlie kangxin granules.METHODS: The sample was determined on DiamonsilTM C18(250 mm?4.6 mm,5 ?m) with the mobile phase consisted of methanol-acetonitrile-water-formic acid(25∶10∶64∶1) at a flow rate of 1.0 mL?min-1.The detection wavelength was set at 286 nm.RESULTS:The linear range of salvianolic acid B was 0.206～1.854 ?g(r=0.999 9).The average recovery rate for salvianolic acid B was 98.0%(RSD=1.5%,n=6).CONCLUSION: The established method is simple,reliable,stable in results and reproducible,and it is applicable for the quality control of Qianlie kangxin granules.

Objective To discuss the therapeutic effects of rhubarb and natrii sulfas for severe acute(pancreatitis)(SAP).Methods Eighty-three patietns with SAP were randomly divided into treatment group(group A,n=45) and control group(group B,n=38).The 2 groups received the same therapy except that group A received rhubarb and natrii sulfas through nasogastric tube.Results In treatment group(compared) to control group,the clinical symptoms such as abdominal pain and distention were more quickly(relieved),with early return to oral food intake,lesser complications and shorter hospital stay(all with P

Objective To explore the factors related with difficulty of laparoscopic cholecystectomy(LC) for acute calculus cholecystitis.Methods A total of 245 patients were retrospectively divided into two groups:easy group(123patients)and difficult group(122patients,including 32patients of conversion to OC)based on surgery time and LC or OC.Results There were statistical differences between the groups in regards to preoperative body temperature,white blood cell count,surgical timing,the thickness of gallbladder wall,gallbladder volume,and diameter of common bile duct(all P

AIM To investigate antitumor effects of curcumin (Cur) combining with adriamycin (ADR) on human tumor cell lines in vitro and study the mechanisms. METHODS The antitumor effects of the drugs on tumor cell lines in vitro were determined with MTT method. The Jin's formula was used to analyze the effect of drug combination. Cellular ADR accumulation was measured by fluorescence spectraphotometry. RESULTS In simultaneous adiministration Cur 4 25～34 00 ?mol?L -1 combining with ADR produced a simple addition or potentiation effect. The cellular ADR accumulation was markedly increased in the presence of Cur in MDR cells, but decreased in sensitived cells. The experiment of membrane fluidity show that that Cur affects membrane fluidity neither on KB nor on KB v200 cell line. CONCLUSION Simultaneous adiministration of Cur combining with ADR produces synergistic effect. The mechanism is connected with increasing cellular ADR accumulationon on KB v200 cell line. While on KB cell line, it is related to synergistic pharmacological effect. But the action of Cur is not connected with the change of membrane fluidity on both cell lines.

Aim To investigate the role of endothe-lium in the enhancement of phenylephrine-mediated vasoconstriction by bupivacaine in the isolated rat aor-ta.Methods The isolated rat aortic rings were pre-pared, and the vascular endothelium was removed chemically or physically .Phenylephrine-mediated vas-oconstriction was recorded .Results A pretreatment with bupivacaine at 30 μmol · L-1 for 20 min signifi-cantly increased the Emax value of vasoconstrictive re-sponses to phenylephrine from 2.22 ±0.07 g of sol-vent-controlled group to 2.50 ±0.05 g ( P<0.01 ) in the isolated endothelium-intact rat aorta.However, the Emax value was not significantly changed by a pretreat-ment with bupivacaine at 30 μmol · L-1 for 5 , 10 or 15 min ( P>0.05 ) .A pretreatment with bupivacaine at 30 μmol · L-1 for 20 min slightly but significantly inhibited the vasoconstrictive responses to low concen-tration of phenylephrine in the isolated endothelium-de-nuded rat aorta (P<0.05).In the isolated endotheli-um-intact rat aorta under a combined treatment with in-dometacin, ChTX, apamin and L-NAME, the vasodi-lator responses to acetylcholine were completely sup-pressed , and a pretreatment with bupivacaine at 30μmol· L-1 for 20 min did not significantly affect the vasoconstrictive responses to phenylephrine ( P >0.05 ) .Conclusion Bupivacaine enhances α1-adre-noceptor-mediated vasoconstriction by inhibiting vascu-lar endothelium in the isolated endothelium-intact rat aorta, Which potentiates indirectly the vasoconstrictive responses to phenylephrine .

Objective To analyze the relationship between plasma concentration and efficacy , adverse drug reactions by monitoring vancomycin serum concentrations for appropriately treating the infections caused by methicillin‐resistant Staphylococcus aureus or other gram‐positive cocci .Methods Vancomycin concentration was monitored in the patients with indications for vancomycin therapy .Blood sample was taken after vancomycin was administered for at least 4 doses .The blood sample collected within 30 minutes before dosing was used to determine the trough blood concentration .The samples were taken within 30 minutes to 1 hour after infusion of vancomycin were used to estimate the peak concentration by fluorescence polarization immunoassay .The clinical data were collected at the same time to analyze clinical efficacy and safety .Results Vancomycin trough concentration ranged from 3 .22 mg/L to 50 .79 mg/L in 25 patients ,specifically ,＜ 5 mg/L in 3 cases ,5‐＜10 mg/L in 11 cases ,10‐15 mg/L in 3 cases ,and > 15 mg/L in 8 csaes .Peak concentration ranged from 13 .57 mg/L to 60 .47 mg/L ,specifically ,＜ 25 mg/L in 14 cases ,25‐40 mg/L in 7 cases ,and > 40 mg/L in 4 cases .The infection was cured in 80 .0% (20/25) of the patients .The gram‐positive cocci were eradicated in 87 .5% (21/24) of the patients .The dosage of vancomycin was adjusted in 13 patients according to the results of blood concentration monitoring .Majority of these patients (12/13 ,92 .3% ) were cured .Renal impairment was observed in 4 patients .Conclusions Vancomycin is safe and effective in treatment of methicillin‐resistant Staphylococcus aureus and other gram‐positive bacterial infections . Vacomycin concentration varies from person to person . Serum concentration monitoring is required to achieve best outcomes and the goal of individualized treatment of vancomycin.