To solve the problems in the illumination of the portable video monocular telescope for near-obser-vation. Some luminous lamp head and reflective lamp head were customerized for the telescope, and the lumi-nous lamp head was postpositively mounted at the side of the ocular lens, which or the reflective lamp head could be prepositively mounted at the side of the object lens. A wound pit on fresh pitaya was observed using portable video monocular telescope for near-observation equipped with illumination device or not. Luminous lamp head and re-flective lamp head custom-made was able to mount easily and gave the target ample illumination. Portable video monocular telescope for near-observation can be easily and cheaply equipped with luminous device giving ample il-lumination.

Objective · To isolate phages which can fight against extended-spectrum β-lactamase (ESBLs)-producing Escherichia coli (E. coli), and provide basic research for establishment of E. coli phage library and treatment of bacterial infection. Methods · Samples collected from sewage were co-cultured with 93 ESBLs-producing E. coli strains. A phage named JDEC001 was isolated by double agar overlay plaque assay. The biological characteristics, complete genome sequence and comparative genome analyses of JDEC001 were studied respectively. Results · JDEC001 belongs to the lytic phage as a member of the Caudovirales order, Podoviridae family. It has high activity at pH from 5 to 11 and with temperature from 0 to 39 ℃ .Whole-genome sequencing of JDEC001 demonstrated double-stranded DNA genome of 38745 bp with GC content of 49.93%, which encoded 46 open reading frames. The comparative genomics also showed that there was no virulent genes or antibiotic resistant genes in its genome. Conclusion · The phage JDEC001 against ESBLs-producing E. coli was isolated and purified, with good stability in a broad range of pH and temperature.