Objective To investigate the correlations between the occurrence of carotid artery atherosclerosis and chlamydia pneumoniae (Cpn) and cytomegalovirus (CMV).Methods Carotid color ultrasonography showed that the patients with intima-media thickness (IMT) ＞ 1.5 mm were enrolled and were used as a carotid atherosclerosis group,and the healthy subjects (IMT ＜ 1.0 mm) were used as a control group.Enzyme-linked immunosorbent assay (ELISA) was used to detect the antibody levels and positive rates of the serum anti-Cpn and anti-CMV IgG.The demography,vascular risk factors,anti-Cpn and anti-CMV IgG antibody positive rates of the carotid atherosclerosis group and the control group were compared.Results A total of 92 patients of the carotid atherosclerosis group were enrolled,including 30 patients with stable plaque and 62 with unstable plaque; a total of 49 healthy subjects of the control group were enrolled.There were no significant differences in the proportions of age,male,hypertension,diabetes,hyperlipidemia,and smoking patients between the carotid atherosclerosis group and the control group (all P ＞0.05).The anti-Cpn IgG (69.5％ vs.26.5％ ; x2 =23.887,P ＜ 0.001),anti-CMV IgG positive rate (75.0％ vs.30.6％ ; x2 =26.156,P ＜ 0.001),and anti-Cpn IgG + anti-CMV positive rate (51.2％ vs.10.2％ ;x2 =24.006,P ＜0.001) of the carotid atherosclerosis group were significantly higher than those of the control group.In the atherosclerosis group,there were no significant difference in the proportions of age,male,hypertension,diabetes,hyperlipidemia,and smoking patients between the unstable plaque subgroup and the stable plaque subgroup (all P＞ 0.05).The anti-Cpn IgG (80.6％ vs.46.7％; x2=11.025,P=0.001),anti-CMV IgG positive rate (83.9％ vs.56.7％;x2 =7.980,P=0.005) and anti-Cpn IgG + anti-CMV positive IgG rate (44.6％ vs.7.6％ ; x2 =10.210,P =0.006) of the unstable plaque subgroup were significantly higher than those of stable plaque subgroup.Conelusions The occurrence of carotid atherosclerosis and the stability of plaque are associated with the Cpn and CMV infection.The Cpn and CMV infection may be the important factors for the occurrence of carotid atherosclerosis plaques and unstable plaques.

Objective To study the effect of arsenic trioxide(As 2O 3) combin ed w ith radiation on the killing of ovarian cancer cells. Methods Using MTT and FCM to detect the cytotoxic and apoptosis at different As 2O 3 concentrations combined with 2 an d 8 ?Gy radiation on ovarian cancer cells(SKOV-3). Radiation survival curves were det ermined by cloning assay with 5?mol/L As 2O 3 combined with 2, 3, 4, 6, 8 and 12?Gy radiation. Curve was used to evaluate the effect of cell killing. Results ⑴ Inhibition of cell proliferation seemed more dependent on the increase of As 2O 3 concentration, ⑵Cell survival rate was lower in the combination of As 2O 3 an d r adiation than As 2O 3 alone, ⑶The apoptosis ratio was increased in 2?Gy and As 2O 3 with increase in As 2O 3 concentration, ⑷D q , D 2 value was decr eased in t he combined As 2O 3 and radiation than radiation only (D q: 1.44 vs 2.78, D 0: 0.85 vs 1.30, SF 2: 0.42 vs 0.87), with radiation enhancement ratio of 1.53 and 2.0 7 according to D 0 value and SF 2. Conclusions Arsenic trioxide is able to enhanc e radiation effect obviously ,especially at lower radiation dose.

Objective To analyze and compare the accuracy and utility of determining sperm DNA integrity by sperm chromatin dispersion (SCD) test and acridine orange staining (AO) test.Methods The level of DNA fragmentation was determined by SCD test and AO test in 32 adult healthy fertile men and 27 idiopathic oligozoospermia (IO) patients.Sperm nuclei with large DNA dispersion halos or with mediumsized halos were normal and nuclei with small-sized halos or no halo were abnormal.The normal sperm DNA was double strands and stained by AO as green.The damaged sperm DNA was single strand,which were stained as green for native DNA and red for denatured DNA.Results The percentage of sperm nuclei with large halos,medium-sized halos,small-sized halos and no halo evaluated bv SCD test in IO patients were (49.9±13.8)%,(11.5±5.4)%,(11.9±6.1)%and(26.7±10.0)%,and they were(73.2±6.2)%,(14.7±6.3)%,(6.8±2.9)%and(5.3±2.2)%in healthy control group,respectively.There was significant difference between two groups(t=8.576,P＜0.01;t=2.083,P＜0.05;t=4.284,P＜0.01;t=11.823,P＜0.01).The percentage of sperm DNA fragmentation was (38.6±12.1)%in IO patients and was (12.1±5.2)% in fertile men,respectively.A statistically significant difference was found between IO patients and healthy control group under SCD test (t=11.995,P＜0.01).AO test showed no significant differences between IO patients and healthy control group (t=1.626,P＞0.05).The percentage of sperm DNA fragmentation evaluated by AO test in IO patients was (45.5 ±13.8)%,and it was (39.8±13.3)%in healthy control group.Conclusions Sperm DNA fragmentation may lead to male infertility.The SCD is effective in testing the sperm DNA fragmentation as a screening procedure to determine semen quality during basic infertility investigation for clinical use.

Objective To detect the expression of CD 44 +/CD24 -and E-cadherin and vimentin in non-small cell lung cancer and its clinical prognostic relevance .Methods By immunohistochemistry , detecting , 500 cases of non-small cell lung cancer E -cadherin ,Vimentin and CD44 +/CD24 -expressions′correlation ,we analyzed the relationship with non -small cell lung cancer .Results In non-small cell lung cancer , vimentin was related to tissue differentiation,TNM staging(P<0.05);there was relationship between CD44 +/CD24 -and EMT by E-cadherin expression(P <0.05);CD44 +/CD24 -expression was related to lymph node metastasis , differentiation(P<0.05);their expressions were not obviously related to age ,sex,smoking history,clinical patho-logical type .The survival time of CD44 +/CD24 -high expression in NSCLC was significantly shorter than lower expression in NSCLC(P<0.05).Conclusion EMT malignant phenomenon raises cancer stem cells ,which can lead to the development of malignant cells poorly differentiated trends ,they are not an isolated process;important role in the development of EMT and CD 44 +/CD24 -union occurs in non -small cell lung cancer and prognosis with patients ,which provides a potential breakthrough in the treatment of cancer prevention and treatment targets .

OBJECTIVETo investigate changes in sperm chromosome and sperm DNA integrity of infertile males.

METHODSThe level of DNA fragmentation was determined by Sperm Chromatin Dispersion (SCD) test in infertile males with idiopathic severe oligoasthenozoospermia (ISOA, n= 19), couples with unexplained recurrent miscarriage (URM, n= 38) and adult healthy fertile men (control group, n= 32). Multi-color fluorescence in situ hybridization (FISH) was performed with probes specific for chromosomes 13, 18, 21, X and Y in the control group (n= 5), the ISOA (n= 10) and the URM (n= 12).

RESULTSPatients with ISOA and URM showed a significantly higher abnormality with total rate of 4.02% (n= 19) and 3.91%(n= 38) for chromosomes 13, 18 and 21, and 2.03%, 1.98% for chromosomes X and Y, respectively, in their spermatozoa compared to control (1.29% and 0.61%, P< 0.01). A significantly higher proportion of total sperm DNA fragmentation was detected in patients with ISOA (40.7%+/- 17.8%) and URM (22.1%+/- 10.3%) of sperm compared to the control group (12.1%+/- 5.2%, P< 0.01). Moreover, a positive correlation was found between the rate of sperm chromosomal aberration and the rate of sperm DNA fragmentation (gamma = 0.874, P< 0.01, n= 27). There were significant correlation between sperm DNA fragmentation and sperm density, sperm motility and abnormal sperm (gamma = - 0.571, gamma = - 0.616 and gamma = 0.637, respectively, P< 0.01).

CONCLUSIONThe result indicates that spermatozoa from patients with ISOA and URM contain greater DNA fragmentation and chromosomal aneuploidy and may lead to male infertility. Screening for sperm DNA damage may provide useful information in the diagnosis of male idiopathic infertility.

Abortion, Habitual ; pathology ; Adult ; Chromatin ; metabolism ; Chromosome Aberrations ; DNA Fragmentation ; Female ; Humans ; In Situ Hybridization, Fluorescence ; Infertility, Male ; genetics ; pathology ; physiopathology ; Male ; Sperm Count ; Sperm Motility ; Spermatozoa ; metabolism ; pathology