Objective:Toconstruct the shRNA(Short Hairpin RNA)eukaryotic expression vector targetingat IER3IP1 gene and observe its silencing effect on IER3IP1 gene.Methods:Two pairs of DNA fragments targeting at IER3IP1 gene and one pair of DNA fragment of negative control were synthesized and cloned into the Eukaryotic Expression Vector pGenesil-1.These vectors were separately transfected into the L02 cells.Inhibitory effect at the time of 24,48,72 h after transfection were detected by semiquantitative RT-PCR.Results:These shRNA eukaryotic expression vectors were successfully constructed.One of them could significantly and specially inhibite the expression of IER3IP1 gene and the inhibition ratios were separately 64%,79%and 69%at the time of 24,48 and 72 h,compared with empty vector group.Conclusion:The combinated eukaryotic expression vector could inhibit the expression of IER3IP1 gene,which forms the basis for investigating IER3IP1 gene's function.

Objective To mine the relation between leukemia and genes using Weka. Methods The papers on leuke-mia and genes were retrieved from PubMed, their subject headings and subheadings were extracted using BICOMB to generate co-occurrence matrix and term-paper matrix. The research hotspots were found by cluster analysis of the data on co-occurrence matrix using Weka and Cobweb. The literature was verified. Results The 42 high fre-quency words were clustered into 7 classes by Weka. No high frequency words of leukemia or genes were found in classes 1, 2, 4 and 5, indicating that their clustering efficiency was poor. The clustering efficiency of the other 3 classes was good. Conclusion Cluster analysis showed that leukemia is related with myc gene, ab1 gene, p53 gene, virus gene, immunoglobulin gene and mdm gene.

Objective To explore the correlation of urine albumin and creatinine ratio(UACR)and short -term prognosis in patients with acute ischemic stroke (AIS).Methods 112 patients with AIS were selected as the research subjects.The admission UACR and related laboratory examination indexes were detected,the US national institutes of health stroke scale (NIHSS)scores,and 28 d after admission of the modified Rankin scale (mRS) prognostic score were recorded.The NIHSS 5 or more points and NIHSS <5 points,different levels and UACR patient observation index between the different prognosis group were compared.Results NIHSS 5 or more group of patients on admission UACR level[(15.41 ±3.21 mg/g)]was higher than the NIHSS <5 patients (9.52 ±2.04)mg/g (t =12.214,P <0.001);UACR acuity 11.60mg/g group in NIHSS of 5 or more proportion of patients (87.5%),mRS score >2 points (82.1%),the proportion of the recent death patients (23.2%)than the proportion of UACR <11.60mg/g,the proportion of the group (32.1%,25.0%,7.1%;the chi -square values 33.509,34.476,5.598,all P <0.050);MRS score >2 groups of patients UACR level[(16.10 ±3.29)mg/g]was higher than that in patients with mRS score 2 points or less (8.38 ±1.95)mg/g(t =20.132,P <0.001);Pearson correlation analysis showed that the standard of UACR and NIHSS score (r =0.412,P <0.05 ),high -sensitivity C -reactive protein (hs -CRP)(r =0.473,P <0.050),white blood cell count (WBC)(r =0.442,P <0.050),fasting plasma glucose (FPG)(r =0.375,P <0.050)were positively correlated.Multiariable Logistic regression analysis showed that UACR (OR =1.587,P <0.050)was a recent independent risk factors of poor prognosis in patients with AIS. Conclusion UACR the illness severity and recent prognosis of patients with AIS is closely related to high levels of UACR may be recent independent risk factors of poor prognosis in patients with AIS.

Objective: To assess the mental health status of poor medical students.Methods: 40 poor medical students and 40 ordinary medical students were studied controlly with symptom checdlist-90. Results: poor medical students than ordinary medical students had more scores in factors and all average. Conclusion: the mental health status of poor medical students is not good.The work of mental health education and consultation should be accessed according to their mental status for them.

ObjectiveTo explore the effect of Songlingxuemaikang combined with losartan on the blood pressure and quality of life in female patients with menopausal hypertension.Methods87 female patients with menopausal hypertension were randomly divided into combined group (Songlingxuemaikang combined with losartan group,44 cases) and the control group(losartan alone,43 cases).Before and after treatment the ambulatory blood pressure (ABMP) was monitored for 24h,and blood lipids and clinical symptoms were observed.ResultsAfter two months treatment,the blood pressure variability and nighttime blood pressure decrease rate of the combination group significantly reduced,and better than those of the control group( all P ＜0.05) ;The effective rate and efficiency rate of the combination group were 90.9％,63.6％ respectively,and was higher than 74.4％,39.5％ in the control group( x2 =4.15,5.06,all P ＜ 0.05 ).The clinical symptoms improving,total cholesterol ( TC ) reducing,density lipoprotein (LDL-C) lowing in the combination group was superior to the control group( all P ＜ 0.05).ConclusionSonglingxuemaikang combined with losartan in treatment of female patients with menopausal hypertension had a good antihypertensive effect,and could reduce blood pressure variability in menopausal women,improve the symptoms of menopause and lipid metabolism,incresse the quality of lfie of patients with menopausal hypertensive.

ObjectiveTo observe the clinical efficacy of warming-unblocking Yin Heel Vessel plus rehabilitation training in treating post-stroke strephenopodia, for seeking the optimal treatment protocol.MethodNinety eligible subjects withpost-stroke strephenopodia were randomized into an acupuncture group, a rehabilitation group, and a united group, to respectively receive warming-unblocking Yin Heel Vessel, rehabilitation treatment, and warming-unblocking Yin Heel Vessel plus rehabilitation training. ResultTwo treatment courses later, there was no significant difference in comparing the strephenopodia score between the acupuncture group and rehabilitation group (P>0.05); the strephenopodia score in the united group was significantly different from that in the acupuncture group and rehabilitation group (P<0.05), and the improvement in strephenopodia in the united group was superior to that in both acupuncture group and rehabilitation group.ConclusionWarming-unblocking Yin Heel Vessel plus rehabilitation can produce a content efficacy in treating post-stroke strephenopodia.

Young faculty are an essential part of a university's strength and is important for its sustainable development.And their scientific research capability is associated with the long-term development of the university.At present,there are 4 major problems with the development of young faculty's research capabilities,which include their relatively single academic knowledge structure,insufficient experience in writing proposals,insufficient opportunities for academic exchange,and inequity in resource allocation.Establishing a training mechanism may help solve some of the problems and promot their development.The research management department of a university must abandon erroneous traditional management concepts,provide research training seminars,develop a new mechanism to stimulate academic exchange,design a system for short-term laboratory rotations,and promote the participation of your faculty in major research projects.These may bring about marked improvement in the improvement of young faculty's research capabilities.

To observe the pathological changes of vascular aging in spontaneously hypertensive rats (SHRs), and the effects of extracts from Panax ginseng, Panax notoginseng (Burk.) and Ligusticum chuanxiong on vascular aging.

BACKGROUND:Bronchial asthma is considered general y to have an association with Th2 immune response disease, but there is no ideal treatment. Bone marrow mesenchymal stem cel s as a kind of adult stem cel s not only have the multipotent differentiation and proliferation capacity, but also have low immunogenicity and immunoregulation ability. OBJECTIVE:To investigate the effect of culture supernatant of bone marrow mesenchymal stem cel s on lung inflammation of bronchial asthma mice. METHODS:Twenty experimental mice were randomly divided into control and experimental groups, 10 mice in each group. At 0 and 14 days, intraperitoneal injection of ovalbumin induced sensitization in mice, and at 24-26 days, aerosolized ovalbumin solution was used for excitation. From the 24th day, in the experimental group, bone marrow mesenchymal stem cel supernatant was intraperitoneal y injected at 2 hours before excitation;meanwhile, normal saline was injected in the control group. At the last of excitation, the mice were sacrificed under anesthesia to take serum samples, bronchoalveolar lavage fluid and lung tissues. RESULTS AND CONCLUSION:(1) Mice in the control group appeared to have abnormal lung tissue structure, and there were a large amount of eosinophils and monocytes in the submucosa and muscularis. However, lung inflammation was relieved in the experimental group after bone marrow mesenchymal stem cel treatment. (2) The levels of interleukin-17 in the bronchoalveolar lavage fluid and serum were significantly lower in the experimental group than the control group (P<0.05), but there was no difference in the levels of interleukin-4 between the two groups (P>0.05). These findings indicate that the intraperitoneal injection of bone marrow mesenchymal stem cel s can ease lung inflammation and reduce levels of inflammatory markers in the bronchoalveolar lavage fluid and serum of bronchial asthma mice.

OBJECTIVE:To discuss the issue of how to take advantage of intellectual property law to promote the sus?tainable development of our traditional Chinese medicine,and to establish the related legal system to protect the intellectual property of traditional Chinese medicine.METHODS:On the basis of literature review,the necessity and foundation of establishing distinctive protective law on traditional Chinese medicine were discussed.RESULT:There were internationally precedents of legislation on biodiversity and cultural diversity;Neither“Trade-Related Aspects of Intellectual Property Rights”nor other WTO files forbad individual country to establish its own policy according to its specific situation,however,these policies should not be against the fundamental principle of WTO;Moreover,foreign countries is paying much more at?tention on legislation of traditional Chinese medicine and herb than they used to.DISCUSSION:It is of great necessity to establish the distinctive protective law on traditional Chinese medicine,which can best be enforced by promoting the self de?velopment of traditional Chinese medicine,especially the investment and innovation in this field.