Objective:To construct the Resuscitation-promoting factor A(RpfA) expression plasmid of Mycobacterium tuberculosis, and express and purify it in E.coli.Methods:RpfAgene of Mycobaeterium tuberculosis was amplified with PCR method from the H37Rv,cloned into pET32a(+) vector,and then transformed into E.coli Top10.After identifying by restriction digestion and DNA sequencing,the constructed recombinant plasmid was transformed into E.coli BL21,and expressed the recombinant protein under the control initiated by T7 after induction with IPTG.The target protein was identified by His-tag in-gel Stain and purified by using affinity chromatograghy.Results:The size of the RpfA gene digested by restricted enzymes accorded with the theoretical size and the DNA sequence with the reported one in literatures.66ku protein was found by SDS-PAGE and His-tag in-gel Stain.Conclusion:The recombinant expression plasmid pET32a(+)-RpfA was successfully cloned and constructed, and the recombinant protein with high purity was obteined;which laid the basis for further study.

Objective:To construct the Resuscitation-promoting factor B(RpfB)expression plasmid of Mycobacterium tuberculosis, and express and purify it in E.coli.Methods:RpfB gene of Mycobacterium tuberculosis was amplified with PCR method from the H37Rv,cloned into pET32a(+)vector,and then transformed into E.coli Top10.After identifying by restriction digestion and DNA sequencing,the constructed recombinant plasmid was transformed into E.coli BL21,and expressed the recombinant protein under the control initiated by T7 after induction with IPTG.The target protein was identified by Western-blot and purified by using affinity chromatograghy.Results:The size of the RpfB gene digestedby restricted enzymes accorded with the theoretical size and the DNA sequence with the reported one in literatures.61KDa protein was found by SDS-PAGE and Western-blot.Conclusion:The recombinant expression plasmid pET32a(+)-RpfBv of RpfB gene was successfully cloned and constructed,and the recombinant protein with high purity was obteined;which laid the basis for further study.

ObjectiveTo explore the causation of nosocomial infection in disabled children.MethodsThe causation of nosocomial infection in disabled children (n=1014) was analyzed retrospectively. The relationship between nosocomial infection and the factors, such as constituent ration in the infections parts, time in hospital, age and contact history separately were analyzed.ResultsOut of the 1014 patients, 291 cases were infected (the rate was 29%). The main site was in the respiratory system (276 cases, 94.8%). Among that, 73.2 % in upper respiratory tract and 21.6% in lower respiratory tract. The infection of digestive canal was only 7.6 %. The nosocomial infection rate in children under 1, 1～3, and above 3 years old were 44.6%, 37.8% and 27.5% respectively. The rate of those with contagious contact history was 31.6%, significantly higher than the ones without contact history (9.1%) (P≤0.05). There was no significant difference between infection rate and the time in hospital.ConclusionThe nosocomial infection is related to the age, contagious contact history, hospital environment and body nutritional situation, but not with time in hospital.

Basement Membrane ; pathology ; ultrastructure ; Biopsy ; Glomerulonephritis, IGA ; pathology ; Humans ; Kidney ; pathology ; ultrastructure ; Kidney Glomerulus ; pathology ; ultrastructure ; Lupus Nephritis ; pathology ; Microscopy, Electron, Transmission ; Paraffin Embedding ; Specimen Handling ; methods

Objective To accomplish the requirement analysis and design of database for electronic medical tag information system. Methods According to the principle and requirement of information engineering methodology, the operation requirement and data requirement were analyzed, and the database was designed including its concept, logic and physics models. Results The thought and method of electronic medical tag information database were proposed. Conclusion A basic data platform is established for casualties' information collection, storage, transmission, exchange and utilization in wartime.

Objective Taken examination of patients with colon polyps by using narrow-band imaging (NBI) com-bined with magnified endoscope, to compare the difference in the opening of the gland morphology and pathological type. Methods 110 patients of colon polyps with 147 pathological histology biopsy samples collected. Using NBI combined with magnified endoscope techniques observe pit patterns and capillary patterns identify adenomatous polyps, and compared with the results of pathological histology, then analyze the adenomatous polyp diagnosis coin-cidence rate, sensitivity and specificity. Results The pit patterns of adenomatous polyps were mostly type Ⅲ, Ⅳ andⅤ. The diagnostic coincidence rate, sensitivity and specificity was 91.16%, 88.37%, 95.08%of adenomatous polyps. It was higher than ordinary colonoscopy (80.27%, 79.07%, 81.97%), and the diagnosis accuracy of intraepithelial neoplasia and early carcinoma of NBI combined with magnified endoscope was obviously higher than that by ordi-nary endoscopy examination, the differences were statistically significant. Conclusions NBI combined with magnified endoscope was superior in observing the opening of pit patterns and capillary patterns, which was helpful to diagno-sis of tumor lesions of colon polyps.

After a description of ISO11620:2014 , the international criteria for library performance assessment , how to enforce the construction of military academic libraries was systematically elaborated in terms of improving the quality and use of their resources and infrastructures, and reducing their construction cost.

FOXP3,a member of the forkhead/winged-helix family,exerts its immunosupression effect by regulating the development,differentiation and maturation of CD4+ CD25+ regulatory T cells(Treg).Treg plays an important role in immune escape mechanism in oncology research.This article reviews the expression of FOXP3 in various types of tumors and its role in tumor immunology and immunotherapy.

A rabbit model of Yang deficiency was established by means of total thyrodectomy and the effect of Decoction for warming kidney and restoring Yang (DWKRY) on the hepatic ultrastructure of the model was observed under transmission eletron microscopy. It was shown in the liver cell of the rabbit model that the number of organelles was decreased, the mitochondria were reduced, degenerated and irregularly distributed, a large number of glycogen granules was stored in the cytoplasm, and the intercellular space was widened. After the administtration of DWKRY, the ultrastructure of liver cells approached normal. This study provides a valuable morphological basis for the establishment of Yang-deficiency animal model and the evaluation of the effect of DWKRY on Yang-deficiency syndrome.

The pharmacologic effects of verapamil (Ver) and phenylephrine(PE)on the smooth muscle of the human fallopian tube in vitro and their relationship to the stages of menstrul cycle were observed respectively. The results demonstrated that both the ampulla and isthmus of the human fallopian tube produced a contractive response to PE, which was antagonized by phentolamine. The PD_2 and PA_2 valucs were larger during the proliferative phase than during the secretory phase in the circular and longitudinal muscle of the isthmus. But there was no difference between the circular and longitudinal muscle. Ver supressed the activity of the human fallopian tuba smooth muscle and antagonized the effects of PE.The PD_2~' values were larger during the secretory phase than during the prolifertive phase. These pharmacologic characte risties of the tube indicated that d-adrenoceptor of the longitudinal muscle of the is thmus may be important, like the circular muscle in the regulation of ovum transport.