OBJECTIVE:To establish the quality standard of Tangkang capsule. METHODS:Momordica charantia L. and Euonymus alatus(Thunb.)Sieb.,the main ingredients in Tangkang capsule including were identified by TLC, meanwhile, the content of total Momordica charantian saponin was determined by UV-Vis. RESULTS: The TLC spots were clear and the separation degree was good. The linear range of Ginsenoside Rg1 was 72～252 ?g(r=0.999 7) with average recovery rate at 100.68% (RSD=1.43%,n=6). CONCLUSION: The established quality standard can be used for the quality control of this Tangkang capsule.

Objective To observe the efficacy of bioelectric stimulation of the pelvic floor combined with acupuncture in treating detrusor urine weakness after transurethral resection of the prostate (TURP) in patients with prostate hyperplasia.Methods Forty-seven cases with detrusor urine weakness after TURP were randomly divided into an experimental group and a control group.All of the patients received routine bladder function training and medication.In addition,patients in the experimental group were treated with bioelectric stimulation of the pelvic floor and with acupuncture.The differences between the two groups were compared using the International Prostate Symptom Score (IPSS),maximum urine flow rate (MFR),average flow rate (AFR),detrusor pressure at peak flow (PdetQmax) and residual urine volume in the bladder.Results No significant difference was found between the two groups before treatment,but at two weeks after treatment MFR,AFR and PdetQmax were significantly higher in the experimental group than in the control group,and residual urine volume and IPSS were significantly lower.Conclusion Bioelectric stimulation of the pelvic floor combined with acupuncture is more effective than routine bladder function training and medication alone for alleviating detrusor urine weakness after TURP.

Electromagnetic Fields ; adverse effects ; Humans ; Occupational Exposure ; analysis ; Railroads

Objective To compare the clinical effect of single-row (SR) and double-row (DR)rotator cuff injury repair.Methods Twenty-three patients with rotator cuff injury were randomized into SR group(12 patients) and DR group (11 patients) by ranom digits table.Two anchors and simple sutures were applied in SR group,three anchors and medial mattress sutures were applied in DR group.Clinical function were compared after operation 24 months,included pain visual analogue scale (VAS) scores,Constant scores,American Shoulder and Elbow Surgeons (ASES) scores,University of California at Los Angeles (UCLA) scores,range of shoulder motion (anteflexion,adtorsion conclination,external rotation),operation time and patient's satisfaction were compared.Re-tear rate and clinical function were also analyzed.Results The operation time in SR group was shorter than that in DR group [(115.8 t25.0) min vs.(124.5 ± 19.7)min,P =0.033].The scores of VAS,Constant,ASES,UCLA in two groups had no significant difference (P ＞ 0.05).The full-thickness re-tear in SR group was 2 patients,in DR group was 2 patients,there was no significant difference (P ＞ 0.05).The full-thickness re-tear and part-thickness re-tear in SR group was 7 patients,in DR group was 2 patients,there was significant difference(P=0.041).Furthermore,the scores of VAS,Constant,ASES,UCLA had no significant difference (P＞ 0.05).Conclusion Compared with SR,2-5 cm rotator cuff injury repaired by DR has no obviously advantage.

Objective:To investigate the cycling stability effect of methoxamine at low dose in cesarean section in order to find the optimal regimen for the prevention and treatment of hypotension during cesarean section. Methods:Totally 200 patients with scheduled cesarean section were selected and randomly divided into the experimental group and the control group with 100 ones in each. The ex-perimental group was anesthetized through L3-4 lumbar-peridural puncture immediately followed by the infusion of methoxamine, and the control group was anesthetized in the same way, and only when the blood pressure was dropped by more than 15%, the infusion of me-thoxamine was given immediately (2-3mg diluted to 20ml). The systolic blood pressure (SBP), diastolic blood pressure (DBP), heart rate ( HR) were compared between the two groups at five time points of before spinal anesthesia ( T0 ) , 5 min after spinal anesthe-sia ( T1 ) , 10 min after spinal anesthesia ( T2 ) , 15 min after spinal anesthesia ( T3 ) and the end of the operation ( T4 ) . The umbilical arterial blood gas and the 1-minute Apgar score of the newborn were recorded, and the adverse reactions in the two groups were com-pared. Results:In T1-T3, SBP and DBP in the two groups were significantly lower than those at T0(P<0. 05), and the decrease in the experimental group was more significant than that in the control group (P<0. 05). In T1-T4, HR in the control group was signifi-cantly lower than that in T0 , while HR in the experimental group had no significant difference when compared with that in T0 ( P>0.05), and HR in the control group was significantly lower than that in the experimental group in T1-T3(P<0.05). There were no significant differences in the scores of pH, PO2, PCO2 and Apgar of the newborn between the two groups (P>0. 05). The incidence of adverse reactions in the experimental group was significantly lower than that in the control group (P<0. 05). Conclusion: The prophylactic use of methoxamine through micro pump can maintain the cycling stability of cesarean section with higher reliability and without adverse effects on the newborn.

Objective To explore the methods of first-staged operation and its clinical efficacy in the carcinoma of left colon with obstruction.Methods The clinical datas of 15 cases of patients hospitalized in 1995 to 2005 were retrospectively analyzed.Results Incision infection and liquification occurred in 4 cases.No patient had severe complications such as fistula in anastomosis and infection of abdominal cavity.Conclusion The therapy of incison and anastomosis in the first-staged operation is practicable if we strictly control indications and improve procedures between the pre-and post-operation.

Objective To investigate the clinical application value of gene chip method for detecting human papilloma virus (HPV) DNA genotype .Methods A total of 372 samples of HPV genotype in the gynecological outpatients department of our hos‐pital from September to December 2015 were collected ,including 22 kinds of genotype(18 types of high risk ,4 types of low risk) , meanwhile all samples were performed thinprep cytologic test(TCT) .Results In 372 samples ,223 cases of HPV gene were detec‐ted with the detection rate of 59 .94% ,194 cases(52 .15% ) of high risk type and 194 cases(7 .80% ) of low risk type were detected ;the abnormal rate of TCT was 44 .08% (including atypical hyperplasia ,low‐grade and high‐grade lesion) ,the atypical hyperplasia was 22 .88% ,the detection rate of high‐grade lesion was 5 .10% and which of low‐grade lesion was 18 .01% .In the cervical lesions showing the lesion beyond inflammation ,multiple infection and cervical lesions had significant correlation (P<0 .05) .Conclusion HPV genotyping has higher clinical application in the cervical lesion screening ,moreover which has good correlation with the cyto‐logical change .Conducting HPV genotyping is beneficial to the early screening of cervical lesions .

Objective To explore the clinical effect and safety of levosimendan,milrinone,and dobutamine in treatment of patients with ADHF.Methods All 336 patients with ADHF accepted in Xi'an Center Hospital from April 2013 to April 2016 were selected and divided into group A,B,and C with 112 cases in each group.Patients in three groups were given levosimendan,milrinone,and dobutamine respectively.Then the clinical effect,hemodynamics,serum indexes,and safety of two groups were observed and compared.Results The total efficacy of group A (90.18％) was obviously higher than group B (58.93％) and group C (65.18％) with statistically significance (P ＜ 0.05).PCWP,PAMP,RAP,and SVR of three groups after treatment were lower than before (P ＜ 0.05).And PCWP,PAMP,and SVR of group A were obviously lower than those of group B and C (P ＜ 0.05).RAP among three groups after treatment had no difference.NT-proBNP,ET-1,and NE of three groups after treatment were lower than before (P ＜ 0.05),and group A was obviously lower than group B and C (P ＜ 0.05).Adverse reaction rate of group A was obviously lower than that of group B and C with statistically difference (P ＜ 0.05).Conclusion Levosimendan has better effect and safety than milrinone and dobutamine in treatment of patients with ADHF,which is worth clinical application.

Objective: to study the relationship between immune complex(IC)and the origin of atherosclerosis(AS),and to evaluate the simvastatin's potential effect on immunomodulation. Methods: Thirty eight truebred New Zealand rabbits were randomly divided into four groups: control group,high-cholesterol fed group,simvastatin-protecting group and simvastatin-treatment group.the structural change of artery and the deposit of IC(and their classes)in plaque lesions of AS were analyzed with hematoxylin and eosin stain and immunohistochemistry. Results: plaque lesions of AS were obvious after 8-weeks high-cholesterol feeding.With the development of AS,the IC of IgG accumulated in plaque lesions,while the IC of IgM did not.The immunohistochemical stain was stronger in high-cholesterol feed group than that in the simvastain-intervening groups.The distribution of immune complexes was under the endothelium in the early plaque while mainly localized in the core or the shoulder of mature plaque lesions. Conclusion: The IC of IgG exists in the plaque lesions of AS.The IC is close related to the origin and the progress of AS.Simvastatin can inhibit the formation of IC as well as lowering the lipid levels and regressing the plaque,which maybe related to it's effect on the immunomodulation.

Objective:To investigate the effect of transfection with wild hepaCAM gene on biological behavior of the transitional cell carcinoma of bladder in vitro.Methods:Human TCCB cell line T24 was transfected with pEGFP-N2-hepaCAM,mock plasmid pEGFP-N2 with lipofectamine.Laser confocal microscopy was used to detect the location of hepaCAM in cells.Western bolt was used to determine the expression of target of hepaCAM in stable cells.The cell adhesive and motility ability were tested by cell spreading assay and matrigel invasion assay.Cell proliferation ability was investigated by MTT assay。Results:In well spread cells,hepaCAM localized on the perinuclear membrane,whereas in the cells which contacts,hepaCAM predominantly accumulated at the sites of cell-cell contacts on cell membrane.Cell clone with stable expression of hepaCAM,was acquired.In vitro experiments as cell spreading assays and matrigel invasion assays showed the cell adhesion and cell motility properties of hepaCAM group were apparently enhanced compared with the non-transfection or mock-vehicle groups.The MTT assay showed cell proliferation ability in the hepaCAM group was notably decreased when compared with the non-transfection or mock-vehicle groups.Conclusion:The hepaCAM gene can restrain malignant phenotypes of the human TCCB cells in vitro,and may inhibit the TCCB invasion and metastasis by influencing the function of some adherence and proliferation factors.[