0.05);the annual recurrence rates of hemorrhage of groupⅠand groupⅡ were 35.0% and 15.0% respectively,the recurrence rates of hemorrhage in group Ⅱ was significantly lower than that of group Ⅰ(P

ObjectiveTo observe the effect of combined rehabilitative training on stroke patients in convalescent stage.Methods52 stroke patients with hemiplegia were divided into rehabilitation group (n=26, receiving combined rehabilitative training) and control group (n=26, not receiving combined rehabilitative training). Scores of functional independence measure (FIM) and Fugl-Meyer assessment (FMA) of all patients were assessed when admission and discharge.ResultsFor patients of rehabilitation group, FMA scores when discharge was not significant difference compared with admission, but FIM scores was significantly higher than that of control group (P<0.01).ConclusionCombined rehabilitative training can improve function and living quality of stroke patients in convalescent stage.

Objective To investigate the expression of GDNF in the olfactory ensheathing glia cells of the adult rats and golden hamster for studying the mechanisms of the OECs in the CNS regeneration. Methods The immunohistochemical staining was performed on the slides of adult olfactory bulb from 3 rats and 3 golden hamsters.According to the primary antibody,tissue slides were divided into 3 groups:rabbit polyclonal GDNF,the rabbit polyclonal GFAP and rabbit polyclonal NGFRp75(Santa Cruz Biotechnology)respectively,and followed by the rabbit ABC immunoreactive staining system. Results It revealed that GDNF was expressed by cells in the first two lines of olfactory bulb from rat and golden hamster.The GFAP and NGFRp75 were also expressed in the same position from other two groups of slides respectively.Conclusion The olfactory ensheathing cell could secret GDNF in the progress of mediating neuronal survival and axonal elongation.

Objective To investigate the effect of miR-15b and miR-16 on the expression of vascular endothelial growth factor (VEGF) protein in human embryonic lung fibroblast (HLF) cells under hyperoxia.Methods The expression level of miR-15b and miR-16 was up-regulated and down-regulated in HLF cells by transfection technology, respectively.The expression of VEGF protein in HLF cells was assessed by Western blot.Furthermore, under hyperoxia exposure in vitro, the expression of miR-15b, miR-16 and VEGF protein in HLF cells was analyzed.Results Up-regulation of miR-15b and miR-16 suppressed VEGF protein expression, while down-regulated miR-15b and miR-16 promoted VEGF protein expression.In addition, hyperoxia exposure induced up-regulation of miR-15b and miR-16, but down-regulation of VEGF protein in HLF cells.Conclusions Hyperoxia exposure may up-regulate the expression level of miR-15b and miR-16, but suppress VEGF protein expression.These may contribute to the development of bronchopulmonary dysplasia.

Objective To investigate the systemic effects of Helicobacter pylori (Hp) infection by comparing differential counts of leukocytes in peripheral blood and C-reactive protein (CRP) levels before and after eradication of Hp. Method A total of 158 Hp-positive patients underwent eradication by standard one-week triple therapy were divided into Hp eradicated group(n = 108) and Hp non-eradicated group(n = 50),the populations of peripheral blood leukocytes and CRP levels before and 0 (just after therapy),1 and 12 months after eradication were retrospectively analysed. Results In the eradicated group,blood leukecytes,neutrophils and monocytes decreased significantly after eradication,and the CRP levels were significantly lower than the levels before treatment (P < 0.05 ),but there was no significant change in eosinophils,basophils and lymphecytes (P > 0.05).In the non-eradicated group,there was no significant change in any studied pa-rameter (P > 0.05). Conclusion These findings suggest that Hp infection increase blood leukocytes,neu-trophil and monocyte counts in the peripheral blood and CRP levels,which indicates a significant role of Hp infection in systemic disorders.

Adenocarcinoma ; surgery ; Adenoma ; surgery ; Adult ; Aged ; Female ; Follow-Up Studies ; Gastrointestinal Stromal Tumors ; surgery ; Hemangioma ; surgery ; Humans ; Ileal Neoplasms ; surgery ; Jejunal Neoplasms ; surgery ; Laparoscopy ; methods ; Lymphoma ; surgery ; Male ; Middle Aged

Objective To observe the effect of therapy combined with rehabilitation approach and acupuncture on cerebral trauma during the recovery stage. Methods 48 cases were divided into two proups: 24 cases in treatment group who applied the rehabilitation combined with acupuncture, the other 24 cases in control group who applied acupuncture alone. They were evaluated with Functional Independent Measure (FIM) 3 months after treatment. Results The scores of FIM were significantly difference between these two groups (P<0.05). Conclusion The therapy combined with rehabilitation approach and acupuncture can improve the recovery of cerebral trauma.

Objective As a collaborator of Beijing Institute of Medical Device Testing for value assignment of state standard ma-terial candidate Cystatin-C ,we have used the internationally accepted reference material to assign value for state standard material candidate Cystatin-C ,and help Beijing Institute of Medical Device Testing get Cystatin-C national standard material certificate . Methods According to the target value and operational procedure of international reference material ERM-DA471 ,We have tested 6 dilutions of standard material candidate Cystatin-C on calibrated Hitachi 7180 immunoassay system .Results The results demon-strate good repeatability and commutability ,and have been accepted in calculating the final value for the candidate standard materi-al ,our data has assisted Beijing Institute of Medical Device Testing in passing the criteria and obtaining Cystatin-C national standard material certificate .Conclusion Compared to the data from all participating collaborators ,our results hit right on the target value , and no significant matrix effects have been observed .

Objective To investigate the location and proliferation of human hair follicle stem cells. Methods The expression of keratin 19(K19) in the human hair follicles on the occiput was detected by immunohistochemical staining, and the positive area was located. The follicle epithelium containing the K19-positive area above the hair bulb were cultured with or without mesenchymes (dermal papilla cells, DPCs) at the air-liquid interface of the collagen gel. When the proliferating colonies formed, the distance from the colony to the bottom of hair follicles was measured and the relationship between the K19-positive position and the proliferation colonies was analyzed. The ultrastructure of the proliferation colony was observed by transmission electron microscopy. Results Two portions were found positive for K19 in the outer root sheath of the human hair follicles, the upper one being at the bulge region and the lower one at the outer root sheath above the hair bulb. The proliferating colony formed only when the epithelium of the hair follicle was co-cultured with mesenchymes cells. The statistical analysis suggested the lower portion positive for K19 and the proliferation colonies in the culture were traced to locate at the same site of the human hair follicles. Under electron microscopy, juvenile cells, mature keratinocytes and apoptotic cells were found in the proliferating colonies. Conclusions The human hair follicles may contain two distinct reservoirs for stem cells, which locate in the bulge area and in the region of the outer root sheath above the hair bulb, respectively. Mesenchymes are needed for the proliferation of stem cells. New stem cells, mature keratinocytes and apoptotic cells may be three endings of the stem cells′ proliferation.

Objective To investigate the clinical effect of the treatment of senile chronic superficial gastritis with the treatment of the elderly patients.Methods Collected in our hospital diagnosed by endoscopy in 80 elderly patients with chronic superficial gastritis were randomly divided into control group and treatment group, 40 cases in each group, the control group using ranitidine therapy, treatment group with teprenone treatment, evaluation of 2 groups of drugs on chronic superficial gastritis and therapeutic effect safety.Results The total effective rate of treatment group was 92.5%, control group total effective rate was 75%;the treatment of two patients with total efficiency, the total effective rate in treatment group was significantly higher than the control group(x2=4.50, P<0.05);the average time for improvement of symptoms in the treatment group was(8.32 ±2.06)days, the average time to improve the symptoms of the control group(11.69 ± 2.12)days, there was significant difference between the two groups(t=7.21, P<0.05), the treatment group the incidence of adverse reaction was 5%, the control group adverse reaction rate was 10%, the difference between the two groups was not statistically significant(x2=0.18, P>0.05).Conclusion It is safe and effective for the treatment of chronic superficial gastritis in elderly patients.