Objective: To develop an improved method for the primary isolation and culture of highly purified endometrial glandular epithelial cells in vitro.Methods: Normal endometrial tissues were isolated and cultured in vitro by double collagenase digestion and selective wall-pasting.Results: Highly purified endometrial epithelial cells were successfully isolated and cultured.Conclusion: Epithelial and interstitial cells of the endometrium can be successfully isolated with high purity by double collagenase digestion and selective wall-pasting.

Objective To evaluate the effectiveness,safety,predictability and stability of a new posterior chamber implantable collamer lens with central hole to correct myopia.Methods 111 eyes of 58 patients were evaluated,including uncorrected (UCVA) and best corrected (BCVA) visual acuities,spherical equivalent(SE),cylindrical equivalent(CE),contrast sensitivity,intraocular pressure evaluated preoperatively.75 eyes of 41 patients were implanted with ICL V4c(ICL group) while 36 eyes of 21 patients were implanted with Toric ICL V4c(TICL group).Postoperative observation and adverse events were evaluated over 3 months follow-up.Results 3 months after surgery,the mean UCVA in ICL group was 1.0 or better in 58.67%.The UCVA of 61 eyes (95.31%) was more than BCVA before operation.The mean SE was (-0.26±1.16) D,66.67% of 50 eyes were within ±0.50 D.The safety and efficacy indices were 1.37 and 1.31,respectively.The mean UCVA in TICL group was 1.0 or better in 86.11%.The UCVA of 32 eyes (96.97%) was more than BCVA before operation.The mean SE was (0.08±0.84) D,63.89% of 23 eyes were within ±0.50 D.And the mean CE was (-0.34±0.54) D,66.67% of 24 eyes were within±0.50D.The safety and efficacy indices were 1.32 and 1.30,respectively.The postoperative contrast sensitivity values in two groups were found significantly higher than those of the preoperative eyes.The IOP remained stable over time.Conclusion Implantation of the posterior chamber ICL with a central hole is effective,safe,predictable,and stable for the correction of high myopia.

It has long been noted that dermabrasion, plum-blossom needling or micro-needling, and even ablative fractional CO 2 laser can effectively induce repigmentation of vitiligo lesions resistant to conventional ultraviolet B phototherapy. In addition to trauma-induced increase in the transdermal absorption of drugs, these treatments also initiate wound repair and activate melanocytes in hair follicles or epidermis. Basal layer keratinocytes, vascular endothelial cells and fibroblasts in the wound margins can secrete chemokine CXCL12 to recruit melanocytes or melanocyte stem cells expressing chemokine receptors CXCR4/CXCR7 in the hair follicle bulge or around the skin lesions to move towards the vitiliginous area. This review summarizes progress in repigmentation of vitiligo lesions induced by therapeutic skin trauma.

Objective To study the use of early bile duct to bile duct end-to-end(side) anastomosis for extrahepatic bile duct injuries.Methods The clinical data of 25 patients with extrahepatic bile duct injuries with early bile duct to bile duct end-to-end(side) anastomosis were retrospectively analyzed.Results Bile duct injuries happened in laparoscopic cholecystectomy (n=17),laparoscopic exploration of common bile duct (n=1),laparotomy (n=5),and knife wound (n=2).All of the repairs were successfully carried out.Except for one patient who was lost to follow-up,the other patients were followed for over 2 years.There was no bile duct stenosis.One patient died after 5 months of repair in other hospital because of a recurrent bile duct calculus.Conclusions Early bile duct to bile duct end-to-end(side) anastomosis had a good long-term results.The operation needs to be done by a good surgeon or supervised by an experienced specialist in biliary surgery.A prolonged period of T tube stenting is a prerequisite for success.

Objective To establish the blood glucose fluctuation diabetic rat model by subcutaneous injecting insulin. Method The male Sprague-Dawley (SD) rats were randomly assigned into two groups, normal group ( n =20) and model group ( n =40). Normal group rats were fed with normal diet, model group rats were fed with a high-sucrose-high-fat diet. After 6 weeks, a low dose of streptozotocin ( STZ ,30mg/kg) was injected to induce hyperglycemia in model group rats. And then the model group rats were ran-dom divided into two subgroups, diabetic group ( n = 20) and blood glucose fluctuation group ( n = 20). Blood glucose fluctuation group rats were induced by subcutaneous injecting insulin twice daily. All rats were executed after intervening for 3 months, metabolic cages were used to collect 24-hour urine one week before all rats were executed. Results The average blood glucose levels (MBG), the standard deviation of daily aver-age blood glucose (SDBG) and the largest amplitude of glycemic excursions (LAGE) were significant differ-ent (P<0. 05) among normal group, diabetic group and blood glucose fluctuation group. The 24 hours, pro-teinuria of diabetic group and blood glucose fluctuation group was higher than that in normal group[( 100. 38±7. 99)mg/24h vs (18. 13±1.41)mg/24h, (123. 71±11.23) mg/24h vs (18.13 ± 1.41 ) mg/24h, P <0.01] , and blood glucose fluctuation group was higher than diabetic group[(123. 71 ± 11.23 )mg/24h vs(100.38±7.99)mg/24h, P <0.05] . Conclusions The blood glucose fluctuation diabetic rats model was successfully induced by the method of subcutaneous insulin injection in diabetic rats twice daily.

砄bjective:To analyse the effects of age on the color of natural tooth crown. Methods: The color of 1 944 health anterior teeth of 162 chinese people aged 20 to 73 years old was measured with a fiber optic spectrophotometry system. CIE 1976 L *a *b * and CIE 1931XYZ were applied to analyse the color mesurements. Results:The results showed that in three section of teeth(incisor, midlle, cervical) the values of L * and a *were negativly correlated to aging, that of b * was positively just at cervical and midlle sites. The value of b * was not related to age at incisal site of teeth. Conclusion: The values of L * and a * are related to age negatively.

Objective To investigate induction of the expression of human ?-defensin-2 (hBD-2) mRNA and the changes in activation of I-?B? and NF-?B by IL-1? in the human airway epithelium. Methods Primary human respiratory tract epithelium were stimulated with IL-1? and and/or PDTC. The expression of hBD-2 mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR). The I-?B? protein level in the cytoplasm was determined by Western blot, and the nuclear factor-kappaB (NF-?B) binding activity was analyzed by electrophoretic mobility shift assays. Results The hBD-2 mRNA expression could be detected after 1.5 hours of IL-1? stimulation at concentration of 1?g/L, and the expression of HBD-2 was in a dose dependent manner. Stimulation with IL-1? resulted in a reduction of I-?B? protein levels. The supershifts assays indicated that the p65-p50 heterodimer complexes of NF-?B were involved in the activation of NF-?B. Conclusions I-?B? can induce the expression of HBD-2 mRNA in a dose dependent manner; the p65-p50 heterodimer complexes of NF-?B play an important role in the regulation of hBD-2 gene expression in response to IL-1?.

It is now widely accepted that platelet aggregation plays an important role in physiological hemostasis and pathological thrombosis associated with cardiovascular and cerebrovascular diseases. Anti-platelet aggregation drug research is also a hot spot of current research. The biggest challenge of antiplatelet therapy has been the molecular overlap of the hemostasis and thrombosis, leading to a serious risk of bleeding. Recent studies have emphasized the importance of shear stress generated from blood flow, which will primarily drive platelet activation and aggregation in thrombosis. So if we can take advantage of the differences between the physiological and pathological vascular blood flow environment, the development of selective anti-platelet therapy may be a safer treatment for cardiovascular and cerebrovascular diseases. In this review, we discuss the underlying mechanisms of shear-induced platelet activation. Later, we summarize the effects and mechanisms of compounds and traditional Chinese medicine on shear-induced platelet activation. The aim is to provide a reference for the study of biological pharmacology of traditional Chinese medicine for promoting blood circulation and removing blood stasis.

Objective To explore a simple and effective method of culturing primary human bronchial epithelial cells.Methods Bronchial epithelial cells were obtained from human bronchial tissues obtained from lobectomy or pneumectomy.Epithelial cells were isolated by three methods as fellows:1.proteinase XIV digestion with mechanical scraping,2.proteinase XIV digestion with mechanical stripping,and 3.trypsin digestion with mechanical scraping.Subsequently the cells were cultured in serum-free hormone-supplemented DMEM/F12.The quantity,purity and viability of cells isolated by three methods were compared.The cultured cells were identified with cytokeratins AE1/AE3 by immunohistochemistry.Results The quantity of cells [(9.37?1.62)?105] obtained from proteinase XIV digestion with mechanical scraping was significantly higher than that of proteinase XIV digestion with mechanical stripping [(5.20?0.75)?105](P

Objective To explore the effects of microstructure grinded on the labial face of porcelain fused to metal crown (PFM) in esthetic prosthesis for anterior teeth. Methods 193 patients with single maxillary incisor tooth defects were repaired with 2 crowns for each one. After the crowns being blent colour and molded, the crown A was not grinded particularly on the labial face and the crown B was done according to the records of the microstructure.The patients were allowed to choose the most satisfied one when 2 crowns were cemented temporarily. Results 36 patients chose the crown A and 157 chose B.The difference was significant ( P0.05). Conclusion The crown has great vitality and accordes with the esthetic standards of the patients by way of being grinded microstructure on the labial face.