OBJECTIVE To identify 134 fungus strains and to detect their drug susceptibility in order to instruct clinical treatment.METHODS The colored culture medium isolated fungus was identified by ID 32C.The resistance to antifungal agents,such as amphotericin B,fluconazole,itraconazole and voriconazole,was detected by ATB Fungus 3 automatic analysis system.RESULTS The isolation rate of C.albicans was the highest from 134 fungus strains,the next was C.tropicalis,the non-C.albicans infection assumed an escalation trend.From the result of drug susceptibility of 134 fungus strains,the susceptibilities to amphotricin B,voriconazole and 5-fluorocystine were the highest,the lowest to fluconazole.CONCLUSIONS As a result of the widespread application of hormons,the immunity inhibitors and the antibiotics,the change of fungal infection is increased,It is necessary to make the fungus identification and the drug sensitive experiment in order to instruct correct clinical uses of the antifungal agents.

Despite progress in the understanding of perirectal anatomy and subsequent adoption of total mesorectal excision,local recurrence after curative resection for rectal cancer continues to be a significant concern.Local recurrence of rectal cancer is associated with a wide spectrum of clinical symptoms and morbidities,and decreased quality of life.The management of locally recurrent rectal cancer is challenging because of the altered pelvic anatomy,postoperative adhesion and difficulty in obtaining a definite diagnosis before surgery.Surgery is the mainstay treatment for locally recurrent rectal cancer,and the use of radiation and chemotherapeutic agents provides significant improvement in survival rate and quality of life.

Objective To compile an applicable inventory of academic diligence for middle school students. Method By means of literature analysis and in-deep interview with teachers, an academic diligence inventory was constructed and used for 393 high school students. Such methods as correlation analysis, exploratory factor analysis and reliability analysis were analyzed by SPSS11. 0 for windows XP. Results ① Five principal components of academic diligence were selected, which could be called target monitoring, time investment, task commitment, persistence and concentration. ②Internal consistency reliability and split-half reliability of total scale are 0. 9126 and 0. 9003. Reliability of each subscale are above 0. 7615. Correlation coefficient between subscales and total scale ranged from 0. 672 to 0. 820. That of among subscales ranged from 0. 248 to 0. 521. Conclusion Reliability and validity of the inventory of academic diligence for middle school students are both satisfactory enough to meet psychometric indexes.

Stoma has been used in colorectal surgery for various indications. Stoma type and stoma location are associated with stoma complications and quality of life with a stoma. The question regarding how to avoid stoma complication and improve quality of life should be considered when stoma creation is planned. The benefit of stoma should be balanced with complications brought about by stoma creation.
Enterostomy ; adverse effects ; classification ; Humans ; Postoperative Complications ; Quality of Life

Objective To implement a remote monitor system in a core medical laboratory.Methods Firstly,the Hub of StreamLab laboratory automation system was connected to a wireless router to build a wireless network,and several equipments were also integrated into the wireless network using wireless network cards.Secondly,VNC Server was installed into the control PCs of StreamLab LAS,CA7000 Coagulation Analyzer,DPC IMMULITE 2000 SMS and its chemiluminescence photometer,and remote control function of Dimension RxL Max was activated,then some control software such as VNC Viewer,Q/Remote and PC Anywhere were setup in two agency control computers.Finally,PCAnywhere and VNC Viewer were setup in the remote control computers in the Internet and Intranet,respectively.Results A remote control system with three functions was built successfully in a core medical laboratory.Conclusion The remote control system makes the daily operation and remote maintenance of the equipments easy.

Objective To implement a real-time bidirectional communication function on Siemens StreamLab laboratory automation system, Methods Firstly, the system selecting sample was changed from the default settings and then the communication character string sent by the StreamLab was utilized by the laboratory information system to build up a real-time bidirectional control of the samples assay. Results New functions such as real-time signing in and real-time detection information getting of specimens could be performed by the StreamLab automatically.Conclusion The real-time bidirectional communication between laboratory automation system and laboratory information system can optimize the working flow and improve the work efficiency.

Objective To explore urban middle students' learning strategies, as well as to provide substantial evidences for learning strategies counselling in middle schools. Methods The differences between sexes,among grades and between class types were examined by t test and MANOVA for 466 middle school students. Resukts The participants'total learning strategies level (3.17±0.52) was slightly higher than midpoint "3" of moderate level. Significant score differences of total learning strategies was found between genders((3.11 ±0.54)vs(3.22±0.48), t=2.30, P＜0.05) and among grades ((3.26±0.51)vs(3.09±0.51), t=3.50, P＜0.01). Interaction of grade and class type on learning strategies was significant (F=7.997,P＜0.01). Conclusion Learning strategies level of middle school students exist genders and grades,and grade and cla.ss type have interaction obviously.

Objective To analyze the coincidence situation of the results of neonatal ABO blood group reciprocal stereotypy with crossmatching test of allotype blood and to investigating the limitations of cross-matching test in infant blood transfusion and effec-tive measures for ensuring the neonatal safe blood transfusion.Methods The micro-column gel test was adopted to identify the ABO blood group and conduct the crosshatching test of allotype blood in 1 095 cases of neonatal blood samples.Results Among the 1 095 samples,the detected rates of weak A and weak B antigen were 3.99% and 17.93% respectively,and the weak B antigen was predominant.The negative rates of anti-A and anti-B antibody were 53.72% and 60.70% respectively;in the cross-matching test with allotype blood,the main side without appearing agglutination accounted for 52.87% and weak agglutination accounted for 33.27%,and the secondary side appearing weak agglutination accounted for 9.49%.Conclusion The maturity of antibody and an-tigen and the coincidence rates of group typing and reciprocal stereotypy in the newborns are less than those in the adults;so blood transfusion according to the cross-matching test results has certain limitation;high attention should be paid to the accuracy of neo-natal ABO blood type,the individual blood transfusion strategy in the newborn should be determined in order to avoid hemolytic blood transfusion reaction caused by ABO allotype blood transfusion and ensure the blood transfusion safety in newborns.

Objective To investigate the role and mechanism of signal transducer and activator of transcription 4 (STAT4) in homocysteine (Hcy)-induced proliferation,migration and senescence of human aorta smooth muscle cells (VSMCs).Methods After VSMCs were incubated with Hcy (0-0.50 mmol/L) for various time periods,a CCK-8 assay was performed to determine proliferation activity.Modified Boyden chamber system (Transwell apparatus) was used to examine migration ability.A SA-β-gal staining kit was used to determine senescence status.Western blotting was performed to examine the expressions of STAT4 and phosphorylated STAT4 (p-STAT4) proteins,and ELISA was performed to examine MCP-1 expression.Results Hcy (from 0.05 mmol/L) significantly increased the proliferation of VSMCs (P ＜ 0.05).Hcy (from 0.10 mmol/L) significantly increased the migration of VSMCs (P ＜ 0.05).Hcy promoted the senescence of VSMC in a concentration-dependent manner,and significantly increased VSMC senescence when the concentration of Hcy reached 0.10 mmol/L (P ＜ 0.05).Hcy (from 0.10 mmol/L) treatment significantly increased the expression of p-STAT4 (P ＜ 0.05) and MCP-1 (P ＜ 0.05).Conclusions Hcy increases the proliferation,migration and senescence of VSMCs,probably by the activation of STAT4 pathway.

The etiology and pathogenesis of inflammatory bowel disease are up to now still not clear and definite. Establishing the ideal animal model to study its cause and pathogenesis of this disease is very important. The ideal animal model should have the same manifestation with human inflammatory bowel disease on clinical and pathologic feature etc. In this article, the method, the pathologic character istics and concerning pathogenesis, of a few common useful experiment animal models are discussed. [