Objective To explore the effect of tracing methodology on nursing management of cardiology department. Methods From January 2014 to December 2016, the two departments were divided into two groups: the observation group and the control group, 81 cases in each group. The patients in the control group were given routine nursing management, and the observation group was given tracing methodological nursing management. The two groups were compared in terms of basic care pass rate, incidence of adverse care events, patient's satisfaction level. Result The prevalence of basic nursing care of the observation group was significantly higher than that of the control, the incidence of nursing adverse events was significantly lower and the patient's satisfaction level was significantly higher (P<0.05). Conclusion In the department of cardiology, the tracing methodology can effectively improve the quality of clinical nursing and patient 's nursing satisfaction.

OBJECTIVE:To observe the analgesic efficacy and adverse reactions of fentanyl transdermal in the treatment of advanced carcinoma pain.METHODS:43patients suffering from advanced carcinoma pain were administered with fentanyl transdermal system every3days with the dosage regulated accordingly until the patients feel painless or almost painless in24h.RESULTS:The general remission rate was90.7%for fentanyl transdermal in the treatment of moderate and severe cancer pain.Its main manifestations of adverse reactions were dizziness,drowsiness,nausea,vomiting and constipation,which have low incidence rates and degree of which were also low.CONCLUSION:Fentanyl transdermal system has durable and stable analgesic effect with its distinct way of transdermal slow-release,which is simple in operation and minor in adverse reactions;and it can be used as the substitutes for oral hadro-opioid.

Objective To investigate the drug tolerance of Acinetobacter baumanni and Stenotrophomonas maltophilia . Methods The antimicrobial susceptibility tests for 275 isolates of Acinetobacter baumanni and 107 isolates of Stenotrophomonas maltophilia from 2000 to 2004 were measured by MicroScan WarkAway 96 The chemotherapeutic effects of 18 cases of sequent infection with Acinetobacter baumanni and Stenotrophomonas maltophilia was analyzed. Results The resistance of the two species of bacteria to twelve antibiotics increased obviously during the last five years, especially from 2000 to 2001. The resistant rate of Acinetobacter baumanni to Cefepime、Cefotaxime、Ceftazidime and Ceftriaxonewas was 20~50% in 2000, but raised to 70%~81% in 2004. For Acinetobacter baumanni to Amikacin、Amp/sulbac、Ciprofloxacin、Gentamicin、Tobramycin and Trimeth/Sulfa, the resistant rate was 20%~40% in 2000, while 63~86% in 2004. The lowest resistant rate was to Imipenem, only 7% or so. The resistant rate of Stenotrophomonas maltophilia to Ciprofloxacin、Ceftazidime、ceftriaxone and Tobramycin was 25%、50%、0% and 0%, respectively, in 2000, but in 2004 year, was 76%、76%、95% and 95%, respectively. Stenotrophomonas maltophilia showed a high drug tolerance to other antibiotics. Conclusion To strengthen the monitoring of the antibiotic resistance of Acinetobacter baumanni and Stenotrophomonas maltophilia and the monitoring of sequential infection in Acinetobacter baumanni and Stenotrophomonas maltophilia is very important for clinic so as to choose antibiotic rationally and improve curative effect

ObjectiveTo evaluate the learning curve of utilizing the full endoscopic technique for the surgical treatment of lumbar disc herniation.MethodsFrom August 2008 to February 2009,30 patients with lumbar disc herniation underwent lumbar discectomy by the full endoscopic-only approach were retrospectively analyzed.The patients were divided into three groups of 10 sequential cases each.Group of early time consisted of the first 10 cases,Group of medium term the subsequent 10 cases,and Group of later time the last 10 cases.The clinical evaluation data included operative time,length of hospital stay,leg and back pain visual analogue scale (VAS),and complications.ResultsAll patients were observed prospectively for (1.61±0.22) years.There was no measurable intraoperative bleeding,no postoperative infections and symptomatic recurrences in the three groups.Compared to group of early time,the operative time in group of medium term was significantly decreased(P＜0.05).The patients in group of later time had much less operative time than that in group medium term(P＜0.05).There was no significant difference with length of hospital stay between the three groups (P＞0.05).The improvement of leg and back VAS in each group was similar:there was a significant improvement 3 months post-operation compared with pre-operation (P ＜0.05),but no statistical difference between 3 months post-operation and final foliow-up(P＞0.05).The complication rate was 12.5％ for group of early time,10％ for group of medium term,and 0 for group of later time.There were two cases conversed to an open procedure for group of early time compared with 0 cases in both other groups.ConclusionExcellent clinical and minimally invasive outcomes can be obtained in the surgical treatment of lumbar disc herniation via the interlaminar approach assisted by full endoscopic technique.However,attention must be paid to the steep learning curve with use of this complex technique.Obtaining microsurgical experience and suitable patient selection can help shorten the learning curve and decrease the complications.

BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) can be induced to differentiate into neuron-like cells in new environment after transplantation, and then to replace damaged cells for reconstructing neural circuit. OBJECTIVE: To establish the co-culture system between rat BMSCs and neural cells in vitro, and to study the influence of neural cells on the differentiation of BMSCs into neuron-like cells in the co-culture system. METHODS: The neural cells obtained from Wistar rat fetal brain tissue and BMSCs gained from rat thighbone were co-cultured in Transwell culture plate. The morphological changes of BMSCs were observed and the special markers of neural cells in BMSCs were examined by immunofluorescence on the fifth day of the co-culture. The results were compared with control group which where BMSCs were alone cultured. RESULTS AND CONCLUSION: BMSCs in the neural cells co-culture system extended, were radial, connected each other. Neuron specific enolase immunoreactions showed positive results, showing neuron-like cells. The positive ratio of neuron specific enolase-positive cells was (33.0±10.5)%. However, BMSCs in the control group did not express neuron morphological character. Immunofluorescence exhibited that cells were negative for neuron specific enolase. These indicate that microenvironment provided by neurons improves the differentiation of BMSCs into neuron-like cells.

ObjectiveTo study the expression of telomerase of fibroblasts in the formation and development of scar,and to investgate the relationship between the telomerase activity and the formation and development of scar.MethodsExpression of telomerase was detected by immunohistochemical techniques in 18 specimens from granulation tissue,17 keloid,16 specimens from hypertrophic scar,28 specimens from mature scar,32 specimens from normal skin,and SPSS16.0 statistics software was used to analyze the relationship between telomerase and scar.Results It was shown that the positive expression rate of telomerase in the granulation tissue group was 94.4 ％,that in the keloid group was 58.8 ％,that in the hypertrophic scar group was 18.8 ％, and that in the mature scar and in the normal skin was zero. comparisons between groups,in addition to the normal scar group and normal skin group,other groups were statistically significant differences.ConclnsionsScar formation is a process with multi-factor participation,while telomerase activation is an important factor. Detecting telomerase activity in the development process of scar formation may determine the prognosis.Through the control of telomerase activity in the pathogenesis of scar may become a new approach of scar treatment.

Objective To establish a rabbit posttraumatic acute diffuse brain swelling (PADBS) model and investigate the mechanism of action.Methods Fifty New Zealand rabbits were assigned to control group (n =10) and model group (n =40) according to random number table.The animal model of sinus balloon compression was established under intracranial pressure monitoring by using intracranial pressure probe.The model group was subdivided equally at 1.5 hours after compression,1.5 hours after decompression,3 hours after decompression and 4.5 hours after decompression,for which intracranial pressure,brain water content,pathological mechanism and ultrastructure were measured dynamically.Results The success rate of modeling was 83％ (33/40).Intracranial pressure was (4.9 ± 0.8)mmHg in control group,(50.1 ± 4.3) mmHg in 1.5 hours after compression group,(45.2 ± 1.7) mmHg in 1.5 hours after decompression group,(48.6 ± 2.2) mmHg in 3 hours after decompression group,and (59.1 ±2.5)mmHg in 4.5 hours after decompression group (P ＜0.05).Brain water content was (75.0 ± 0.6) ％ in control gorup,(76.7 ± 0.8) ％ in 1.5 hours after compression group,(77.3 ± 0.5) ％ in 1.5 hours after decompression group,(78.5 ± 0.6) ％ in 3 hours after decompression group,and (79.4 ± 0.7) ％ in 4.5 hours after decompression group (P ＜ 0.05).Vasogenic brain oedema was seen 1.5 hours after decompression.Cytotoxicity brain swelling generated with brain tissue destroyed 3 hours after decompression.The vicious cycle of high intracranial pressure and brain tissue destruction occurred 4.5 hours after decompression.Conclusion Under intracranial pressure probe monitoring,the rabbit model of PADBS by sinus balloon compression has stable pressure of the sinus balloon and has good reliability and repeatability,which provides a reliable evidence for further study on the possible mechanism and treatment methods of PADBS.

OBJECTIVE:To establish the quality standard for Qindan granules. METHODS: The constituents including Radix Scutellariae, Radix Astragali, Cortex Moutan and Fructus Gardeniae in Qindan granules were identified respectively by TLC. RESULTS: The test samples and their respective reference substances were alike in that the same color chromatographic spots were noted at the corresponding places. The color spectra of the samples were clear yet without interference of surrounding impurity. CONCLUSION: The established method has high specificity and can be used for the quality control of Qindan granule.

Objective To investigate the preventive and therapeutic effect of Bushen Jiedu prescription on ionizing radiation induced sex hormone disorder.Methods Totally 72 adult rats with half male and half female were randonly divided into 6 groups,including control group,radiation group,Bushen Jiedu prescription group with male and female,with 12 rats in each group.After adaptive breeding for 2 d,the rats of Bushen Jiedu prescription group were given 0.2 ml water soluble granules of Bushen Jiedu prescription (1.38 g/ml) by gavage once a day for 10 days.At 11 d,the rats were irradiated by 5 Gy of 60Co γ-rays.24 h after radiation,6 rats in each group were executed to measure the level of sex hormone.The other rats were further gavaged with Bushen Jiedu prescription or its control once a day for 14 d,then were executed for sex hormone testing.Results At 1 d after radiation,the estrogen (E2) of female of Bushen Jiedu prescription group was significantly higher than that of radiation group (t =2.686,P ＜ 0.01).Compared with control group,the levels of follicule-stimulating hormone (FSH) (t =4.305,4.642,P ＜0.01) of male and female,the levels of luteinizing hormone (LH) of male radiation group,and Bushen Jiedu prescription group were increased (t =4.537,2.336,P ＜ 0.01).At 14 d after radiation,the levels of testosterone and luteinizing hormone in male rats of radiation group was higher than those in control group (t =-3.730,2.963,P ＜ 0.05),and the levels of E2 and progesterone of female was lower than those in control group (t =-5.424,-3.178,-2.690,-3.215,P＜0.01).Conclusions Ionizing radiation can induced sex hormone disorder,and Bushen Jiedu prescription can effectively inhibit this alteration,which indicates that Bushen Jiedu prescription is an ideal radiation protectant.

Objective Accumulating reports have suggested that hepatic stellate cells (HSCs) exhibit immunosuppressive ability and may be responsible for the occurrence and development of hepatocellular carcinoma (HCC).The mechanisms through which HSCs affect T-cell-induced adaptive immune responses and the relationship with the regulatory T cells (Treg cells) were studied.Methods We isolated HSCs from wildtype mice to demonstrate the influence of HSCs on T-cell proliferation and explored their effect on Treg cells through mixed leukocyte reactions (MLRs) in vitro.Results We found that activated HSCs could induce T-cell hyporesponsiveness in adaptive immune response by inhibiting the proliferation of T cells andincreasing the quantity of Treg cells.Conclusion Activated HSCs may lead to hypoergia of T cells in adaptive immune reaction and up-regulate the expression of Treg cells,thus facilitating immunotolarance.