Objective To investigate the pathological changes of brain tissues from contused and contralateral non-contused sides and their significance.Methods A total of 100 healthy adult Wistar rats were assigned to brain contusion group and sham operation group according to the random number table.Ten rats in each group were sacrificed at 1,3,6,12,and 24 hours respectively.Brain samples were collected to perform pathophysiological analysis of brain tissues and test blood brain barrier (BBB)permeability by semiquantitative immunohistochemical staining of IgG.Results There was no damage to the bilateral brain tissues in sham operation group and IgG stain was negative.In brain contusion group,angioedema characterized by the breakdown of BBB was seen in the contused side at 1 hour followed by cellular edema at 3 hours,with aggravation of both over time.Moreover,tissue necrosis,inflammatory cell infiltration,and microglia proliferation emerged at 12 hours.Besides,IgG-positive staining was seen at 1 hour,was strongest at 6 hours,and remained a high level at 24 hours.With respect to the non-injured side in brain contusion group,no pathological abnormalities and negatively stained IgG were observed at 1 hour; cellular edema and weakly positive-stained IgG were found at 3 hours; aggravated cellular edema,emergence of angioedema,and IgG strongly positive staining were noted at 6 hours;cellular edema continued aggravation,but angioedema tended to be attenuated,IgG staining weakened,and microglia proliferation were observed at 12-24 hours.Conclusion Pathological changes of brain tissues from the contused and contralateral non-contused sides are differed,which provides a basis in determining treatment choices.

Objective To study the effects of violet flower Bidai extracts on body weight,blood lipid level,serum leptin level,the activity of lipoprotein lipase and hepatic lipase of obese rats,and to discuss the mechanism of violet flower Bidai extracts on weight reduction.Methods Male obese rats which induced by high energy diet were randomly divided into four groups.All rats were given high energy diet.One control group was gavaged with normal saline and one western medicine group was gavaged with sibutramine,and two violet flower Bidai extracts groups was gavaged with water solutions of violet flower Bidai extracts at doses of 6 g(crude drug)/kg BW,12 g(crude drug)/kg BW,respectively.After six weeks,body weight,obese index,weight of abdominal fat tissue,fasting lipid,leptin in serum,the activity of lipoprotein lipase and hepatic lipase were measured.Results Body weight,obese index,weight of abdominal fat tissue in the two violet flower Bidai extracts groups were significantly lower than those in obese control group(P

AIM:To evaluate the effectiveness and safety between trabeculectomy with collagen matrix versus trabeculectomy with mitomycin C(MMC) for patients with primary open-angle glaucoma(POAG).METHODS:In this prospective randomized comparative study from January 2015 to December 2016.Thirty-two eyes presented with POAG were included in this study, 14 eyes treated by trabeculectomy with subconjunctival implant of collagen matrix (study group) and the other 18 eyes treated by trabeculectomy with mitomycin C.Postoperative IOP, the success rate of operation, number of postoperative glaucoma medications and postoperative complications were recorded.Each patient was followed up at least 6mo.RESULTS:The mean postoperative IOP was statistically different between the study group and the control group after 1d (P<0.05), while not statistically different at 1 and 1mo follow-up (P>0.05), and the mean postoperative IOP was statistically different between the two groups (P<0.05) at 3 and 6mo after surgery.There was no significant difference in the success rate of operation between the two groups at 6mo after operation (P>0.05).The IOP decreased at 1d after openations compared with before, kept stable at 1wk to 6mo.IOP of study group was lowen than control.IOP was controlled by glaucoma medications in the study group by 28% compared to control group by 33% at 6mo after operation, but there was no significant difference.There was no significant difference between the study group and the control group in complications (P<0.05).CONCLUSION:Trabeculectomy with collagen matrix implant is comparable to the use of MMC with a similar success rate in open-angle glaucoma and the range in reducing intraocular pressure was significantly higher than that of MMC and it can significantly avoid the occurrence of low IOP postoperatively, transient anterior chamber, conjunctival wound leakage complications has no advantages compared with the use of MMC.

Objective This study was designed to investigate the correlation between the apparent diffusion coefficient(ADC)value and pathological change of the non-injured side brain tissue of traumatic brain injury(TBI)of rat.Methods Sixty healthy adult Wist-ar rats were randomly divided into two groups:the control or TBI groups.The TBI group was divided into five sub-groups according to the different time intervals:1,3,6,12,24 h(n = 10).The animal brain of each group was scanned with MR-DWI,and the ADC value of damaged areas and contralateral non-damaged areas were measured.After that the brains were taken out at different time points after TBI.The cerebral edema and blood-brain barrier(BBB)changes in structure were examined with an optical micros-copy and transmission electron microscopy,and the IgG content in the same tissues were determined by means of immunohistochem-istry.The data was analyzed with SPSS 13.0 statistical software.Results There was no signal abnormality on MR-DWI and tissue structure in control group,and the IgG stain was negative.In the contralateral non-damaged areas of TBI group,pathological obser-vation revealed no variation at 1 h after TBI,and the IgG stain was also negative.Cellular edema was shown at 3 h and the IgG stain was slight positive.The cellular edema aggravated with time and angioedema appeared at 6 h.IgG stain was shown significantly posi-tive.At 12~24 h,cellular edema kept increasing more severe,however angioedema had a trend to mitigated along with time,IgG stain became slight and the proliferation of glial cells could observed.Compared with control group,the ADC values of the contralat-eral non-damaged areas in each group showed no significant difference (P >0.05).However,in the damaged areas of each group, angioedema appeared at 1 h and gradually aggravated,cellular edema occurred at 3 h,Both of them were aggravated with time. ADC values increased at 1 h,and then decreased until 6 h,then followed a climbing up to 24 h,showed as a “V”-shaped.Con-clusion When contused on one side brain,the contralateral non-inj ured side tissue also has pathological changes that occurs later than those on the inj ured side.Cellular edema of the tissues ap-peares first and is followed by angioedema,which lessens over time..There is no significant difference of ADC values between con-tralateral side of TBI groups and control group(P >0.05),which reveals a “pseudo-normal”phenomenon.

OBJECTIVETo study the changes of cardiovascular regulating factors in rats during recovery of aerobic exhaustive exercise.

METHODSSixty male Wistar rats were randomly divided into control group, 1 h-exercise group, 3 h-exercise group, exhausted group, 2 h-recovery group and 12 h-recovery group. The rats were killed at corresponding times for each group after an 8-week-long treadmill training, and the levels of NO, ET, ANP and TXB2 in plasma were measured in each group.

RESULTSNO/ET ratio of 1 h-exercise group was significantly higher than that in control group (P < 0.01), while it was significantly decreased in 3 h-exercise group and exhausted group (P < 0.05). ANP contents in rat plasma were significantly higher in 3 h-exercise group, exhausted group and 2 h-recovery group than that in control group (P < 0.05 or P < 0.01). The concentration of TXB2 in plasma was significantly increased in 3 h-exercise group, exhausted group and 2 h-recovery group (P < 0.05).

CONCLUSIONChanges in cardiovascular regulating factors after exhaustive exercise may lead to deficiency of coronary circulation blood/oxygen supply, which may cause exercise-induced fatigue.

Animals ; Atrial Natriuretic Factor ; blood ; Cardiovascular System ; physiopathology ; Endothelins ; blood ; Exercise Test ; Fatigue ; blood ; Male ; Nitric Oxide ; blood ; Physical Conditioning, Animal ; Rats ; Rats, Wistar ; Thromboxane B2 ; blood

Objective To investigate the protective effects of quercetin on primary cultured SD neonate rats cardiomyocyte injury induced by daunorubicin.Methods Cultured cardiomyocytes were divided into blank groups,DNR groups,DNR+QUE groups,QUE groups.After preincubation of cardiomyocytes for 24 hours,cytoprotection effect was assessed by cell morphous,and cell apoptotic rates determined by flow cytometry,extracellular lactate dehydrogenase(LDH),superoxide dismutase(SOD) and malondialdehyde(MDA).Results Compared with DNR groups,the shedded cardiomyocytes and the cell debris of DNR+QUE groups decreased;the cardiomyocyte apoptosis rates decreased;the content of LDH and MDA decreased(P

BACKGROUND: In recent years, epidemiological studies have found that the saturation of transferring or the increased level of serum ferritin are associated with the attacks of cancer, coronary heart disease, diabetes mellitus, Parkinson disease, liver disease and the diseases of immune system. Therefore, it is suggested that the intake of excessive iron may cause adverse influence on the healthy of human body.OBJECTIVE: To establish high-iron model in mice by using full-rate diet pellets by adding regular quantitative intraperitoneal injection of iron dextran, and observe the iron levels in vivo and the changes of organ coefficients.DESIGN: A comparative observation.SETTING: Department of Nutrition and Food Hygiene, School of Public Health and Tropic Medicine, Southern Medical University.MATERIALS: The experiment was carried out in the laboratory of the Department of Nutrition and Food Hygiene, School of Public Health and Tropic Medicine, Southern Medical University in May 2006. Forty Kunming mice of SPF grade, 20 males and 20 females, weighing 18-22 g, were provided by the Animal Experimental Center of Southern Medical University. The mice were randomly divided into control group (n =10) and high-iron model group (n =30) by introperitoneal injection of saline and iron dextran respectively, and the latter group was subdivided into low, middle and high-dosage groups (6.25, 12.5 and 25 g/L) respectively, 10 mice in each group. Full-rate diet pellets (iron content was 370 mg/kg)were purchased from the Animal Experimental Center of Southern Medical University. Iron dextran reagent (norm: 2 mL containing 50 mg iron) was the product of Zhejiang Ruian Pharmaceutical Factory (certification number: H33021758).The kits of superoxide dismutase (SOD) and maldondialdehyde (MDA) were provided by the Nanjing Jiancheng Bioengineering Institute.METHODS: Mice in each group were raised in plastic stainless steel cages respectively at (23±3) ℃, and they were free to the access of food and deionized water. Mice in the low, middle and high-dosage group were treated with intraperitoneal injection of iron dextran once every other day, 0.8 mL for each time, whereas iron dextran was replaced by saline in the control group, all the mice were treated for 6 weeks, and their nutritionol conditions were observed. All the mice were killed at the end of the 6th week. The iron contents in organs of heart, liver, spleen, lung and kidney, and serum were determined with atomic absorption spectrophotometer and automatic biochemical analyzer respectively; Pathohistological examination of organs were performed; The organ coefficients of liver and spleen were calculated; MDA content and SOD activity in serum were determined.MAIN OUTCOME MEASURES: General conditions of mice in each group; Iron contents in organs and iron concentration in serum; Organ coefficients of liver and spleen; MDA content and SOD activity in serum; Pathological changes.RESULTS: In the high-iron model group, the body figures of the mice were changed, body masses were obviously decreased. The iron contents in organs and serum of mice in the high-iron model group were all obviously increased as compared with those in the control group (t =5.841, P ＜ 0.01), the organ coefficients of liver and spleen were also markedly increased (t =5.841, P ＜ 0.01), which were all in a dosage-dependent manner. The MDA content in serum was obviously increased (t =5.841, P ＜ 0.01) whereas the SOD activity was obviously decreased (t =12.924, P ＜ 0.01) as compared with those in the control group. The pathohistological examination under light microscope showed that there were pathological damages of different degree occurred in the tissue and cells and cell degeneration was observed,which affected the normal physiological function of cells.CONCLUSION: High-iron mice models can be successfully established by the intraperitoneal injection of iron dextran.The storage of excessive iron in vivo will result in the organic damages.

BACKGROUND: Iron deficiency anemia (IDA) is one of the highest incidence nutritional-deficiency diseases all over the world; especially infants and children are the main group. IDA presently becomes one of the most important nutritional problems to be solved.OBJECTIVE: To observe the effect of chocolate carrier and orange juice on recovery of IDA model rats.DESIGN: Randomized controlled animal study.SETTING: Laboratory of Nutrient and Food Hygiene, School of Public Health and Tropical Medicine, Southern Medical University.MATERIALS: The experiment was carried out in the Laboratory of Nutri ent and Food Hygiene, School of Public Health And Tropiacal Medicine, Southern Medical University from March to June 2006. A total of 60 healthy SD rats of clean grade were provided by Animal Center of Southern Medical University (certification: 2002-009 2005A047). METHODS: ① Establishment of IDA models: Among them, 20 rats of half genders were randomly selected toregard as control group, and other 40 were regarded as model group. Rats in control group were fed with rou tinefeed and drank freely. Rats in model group were fed with AOAC-modi fied low-dosage iron feeds to establish IDA models by blooding at caudal vein. Three weeks later, average concentration of ferrohemoglobin in model group was decreased to about 90 g/L, and this suggested that model estab lishment was successful. Ten rats of half genders in each group were ran domly sacrificed. Pre-experiment and 3 weeks of post-experiment, rats were weighed to measure concentration of ferrohemoglobin with hemoglobin cyanide (HiCN) technique, red blood cell count (RBC, direct method), serum iron (microparticle chemiluminescent immunoassa y and related kit) and concentration of serum transferrin receptor (STFR, ELISA method and related kit). ② Recovery test: Other 10 rats in control group were regarded as normal control group, and they were fed with routine feed and drank freely. The rest 30 rats of half genders in model group were randomly di vided into 3 subgroups: model control group, FeSO4 group and chocolate & orange juice group with 10 in each group. Rats in model control group were perfused with distilled water everyday; rats in FeSO4 group were per fused with FeSO4, and rats in chocolate & orange juice group were per fused with chocolate carrier and orange juice. The iron volume in the last two groups was 6 mg/(kg·d). At 40 days after intervention, the experiment was stopped. Concentration of ferrohemoglobin, RBC, serum iron, concentration of STFR and activity of plasma-protein aconitase were measured with atom-trapping atomic-absorption spectrophotometry; meanwhile, biological utilization rate of chocolate carrier & orange juice was calculated.MAIN OUTCOME MEASURES: ① Contents of herrohemoglobin, RBC,serum iron and STFR before experiment and after modeling; ② contents of ferrohemoglobin, RBC, serum iron, STFR and activity of plasma-protein aconitase before recovery test and at 40 days after experiment; ③ Related biological utilization rate.RESULTS: All 60 rats were involved in the final analysis without any loss. ① Comparison of blood index after modelling: Content of ferrohemoglobin, RBC and content of serum iron were lower in model group than those in control group (P ＜ 0.01), but content of STFR was higher than that in control group (P ＜ 0.01). ② Comparison of blood index and activity of plasma-protein aconitase in liver before recovery test and at 40 days after experiment: At 40 days after intervention, concentration of ferrohemoglobin,RBC and content of serum iron were higher in FeSO4 group and chocolate & orange juice group than those in model control group (P＜ 0.01); however, content of STFR was lower than that in model control group (P ＜ 0.01).At 40 days after intervention, activity of plasma-protein aconitase in FeSO4 group and chocolate & orange juice group was higher than those before recovery test (P ＜ 0.01). ③ Related biological utilization rate of chocolate carrier plus orange juice: Biological utilization rate of FeSO4 was regarded as 100%, and biological utilization rate of chocolate carrier plus orange juice was increased remarkably (106.7%).CONCLUSION: Chocolate carrier plus orange juice can improve IDA function and wildly use on treating IDA because of its good absorption. It is characterized by well biological utilization rate and good taste; therefore,it is a hot topic for trophology and foods produce presently.

Angiotensin-Converting Enzyme Inhibitors ; therapeutic use ; Cardiotonic Agents ; therapeutic use ; Child ; Diuretics ; therapeutic use ; Heart Failure ; drug therapy ; etiology ; surgery ; therapy ; Heart Transplantation ; methods ; Heart-Assist Devices ; Humans ; Pediatrics ; Treatment Outcome