As an important organ of the human body,vascular endothelial cells (VECs) play a vital role in heat stressinduced tissue damage.Its integrity not only serves as a barrier for maintaining vascular permeability but also has major impact on cellular structure and function during acute phase response to heat stress.In heat stroke,a series of acute and complicated pathophysiological changes,including microcirculation change,damage VECs and thereby induce or aggravate multiple organ dysfunction syndrome (MODS).Meanwhile,studies have shown that,during heat stroke,VECs are the major responding cells and one of the most common cells that experience morphological and functional changes.Therefore,VECs damage might be an important mechanism involved in heat stroke.This article reviews the mechanism of heat-induced damage of VECs and its effect on vital organs.

Rehabilitation therapy with the integrative traditional and western medicine is the devel opment trend in China,and it is also the aim of talent education of Nanjing University of Chinese Medicine.Based on the introduction of rehabilitation therapy education in our university,this article makes a discussion on the research and practice of curriculum reformation of rehabilitation therapy with the integrative traditional and western medicine.Additionally,it puts forward some implement measures.

Objective To observe the effect of theanine in vitro and in vivo, including suppression of lung tumor growth, tumor an-giogenesis and promotion of apoptosis. Methods The inhibitory effects of theanie on lung cancer A549 cells were analyzed by MTT assays. The cell cycle and the apeptotic percentage of A549 cells were detected by FCM. The angiogenesis effect of theanine was observed with CAM model. The inhibitory effects of theanine were observed with lung carcinoma nude mice model, and the immunohistochemic technique was used to investigate the expression of CD34 and VEGF (vascular endothelial growth factor). Results Treatment of A549 cells with VEGF re-sulted in significant dose-dependent and time-dependent inhibition. FCM detection indicated that administration of EGCG resulted in an in-crease in cells in the S phase of the cell cycle and a typical apoptosis peak before the GI phase with an apoptosis rate of 15.9%. There was a significant difference in tumor volume and weight in theanine group compared with control group after two-week treatment, and the tumor in-hibition rate was 43.6%. There was no significant difference in expression of VEGF in tumor tissue according to tumor MVD marked by CD34 between the theanie group and control group. Thus, theanine could obviously inhibit tumor angiogenesis. Conclusion VEGF can ar-rest lung tumor growth via the promotion of tumor cell apoptosis and the inhibition of tumor angiogenesis.

Objective To investigate the effects of photodynamic therapy (PDT) on Staphylococcus aureus and Escherichia coli strains and their laboratory-developed biofilms in vetro and the basic mechanisms of its action.Metods The bacteria were irradiated with a He-Ne laser in the presence of toluidine blue O (TBO).The colony forming units (CFU) of each strain were countde before and after treatment,and the uptakd of TBO by the bacteria was determined using fluorospectrophotometry. Results With a constant light dose,the bactericidal effects of PDT against the two bacteria cultures increasde woth the concentration of TBO,with the strongest bactericidal effect after PDT treatment more than 90%.S.aureus seemed to be more sensitive to PDT than E.coli,though the amount of TBO absorbed by the E.coli cells was significantly larger.The effects of PDT on mature and immature biofilms formed by the two strains showed no significant difference.The bactericidal rate was 20% to 30%.Conclusion Ge-Ne laser irradiation associated with TBO demonstrated significcant photodynamic bactericidal effects on S.arueus and E.coli strains cultivatde in vitro,but the iffects on their biofilms wer limitde.The efficacy of PDT was independent of the absorption of TBO into the bacterial cells.

Objective To investigate the clinical efficacy of acupuncture at Hegu plus Fuliu in treating hyperhidrosis in Parkinson’s disease. Methods Fifty-seven Parkinson’s disease patients with hyperhidrosis were randomly allocated to a treatment group of 28 cases and a control group of 29 cases. The control group received conventional acupuncture and the treatment group, acupuncture at Hegu plus Fuliu in addition. The VAS score and the HDSS score were recorded in the two groups before and after treatment. The clinical therapeutic effects were compared between the two groups.Results There were statistically significant pre-/post-treatment differences in the VAS score and the HDSS score in the two groups (P<0.01). There were statistically significant post-treatment differences in the VAS score and the HDSS score between the treatment and control groups (P<0.01). The total efficacy rate was 96.4% in the treatment group and 72.4% in the control group; there was a statistically significant difference between the two groups (P<0.05).Conclusion Acupuncture at Hegu plus Fuliu can effectively reduce hyperhidrosis in Parkinson's disease patients.

Objective:By analyzing the principle and mechanism of far-infrared radiation therapy, explore the far infrared radiation therapy for arteriovenous fistula function protection significance. Methods: Summarized and analyzed in hemodialysis patients arteriovenous fistula dysfunction causes and hazards, combined with clinical results, analyzed the intervention and protective effect of far-infrared radiation therapy for arteriovenous fistula.Results: Long-term treatment of far-infrared radiation play a very effective role in the intervention and protection for arteriovenous fistula function maintenance.Conclusion: By analyzing the principle and mechanism of far infrared radiation therapy, summed arteriovenous fistula dysfunction reasons. Clarified the far-infrared radiation therapy has a positive clinical significance in hemodialysis patients arteriovenous fistula function of protection.

OBJECTIVE:To establish a RP-HPLC method for the determination of capsaicin in Capsicum annuum. METHODS:The determination was performed on Zorbax Eclipse XDB-C18(150 mm?4.6 mm,5 ?m) column with column temperature set at 40 ℃.The mobile phase consisted of acetonitrile and 0.1% phosphate acid solution (45∶55,pH=2.6) with flow rate at 1.0 mL?min-1 and detective wavelength set at 281 nm.RESULTS:The calibration curve of capsaicin was linear over the concentration range of 30～70 mg?L-1(r=0.999 9).The average recovery for capsaicin was 101.33 %(RSD=1.57%,n=9).CONCLUSION:The method is simple,rapid and accurate,and suitable for the determination of capsaicin in Capsicum annuum.

Objective The special features of discoid meniscus(DM) symptoms， the relationship between the symptoms and the signs of DM, the type and scale of DM injury as well as the prognosis were studied. Methods According to the analysis of 64 cases of DM diagnosed by arthroscopy, a comparitive analysis was made and the following topics were addressed: the distribution of the complete type of DM in patients of different age groups, the signs of locking and non-locking, with or without effusion, snapping and non-snapping, a comparison of the distribution of the snapping in different groups of injury types, a comparison of the distribution of the flexion contracture in different types of injury, a comparison of the distribution of the rates of the total and subtotal menisectomy in different groups divided according to the period of pain, the presence of effusion, the presence of locking, snapping, quadriceps atrophy and extension function. The data was analysed statistically. Results There is significant difference in the distribution of the complete type DM between 20 and over 20-years of age(P0.05). Conclusion The complete type of DM was mostly seen in cases below 20 years of age and was rarely seen in cases with the locking sign. Snapping sign was mostly seen in cases of complicated and extensive injuries and was one of the indications of arthroscopic surgery. Knee flexion contracture was mostly seen in cases of longitudinal and extensive injuries. The estimation of the results of arthroscopic surgery for DM is difficult on the bases of the clinical symptoms and signs.

Objective: To study the influence of inhibitor of differentiation 2 (Id2) on cell proliferation and migration in SKOV3 cells, and to investigate the influence of HLH domain deletion in 1d2 gene on SKOV3 cells. Methods: Human ovarian cancer cell line SKOV3 was transfected with pcDNA3.1-Id2, pcDNA3. 1-Id2-DBM and pcDNA3. 1-Id2-DBM-δHLH vectors by SuperFect Transfection Reagent. The cells transfected with blank vector pCDNA3. 1 were used as control. Id2 protein was detected by Western blot, and mRNA transcriptions of Id2, Id2-DBM (D-box mutant), and Id2-DBM-δHLH were measured by RT-PCR. Cell proliferation was assessed by MTT assay. Cell invasion was detected by scratch test and transwell chamber assay. The regulatory effect of Id2 on E-cadherin was evaluated by Western blot. Results: The mRNA and protein levels indicated that the plasmid was successfully transfected. Cell growth curve suggested that the proliferation of cells had no significant difference between each group. Compared with the control group, cell migration ability was significantly enhanced after transfection with pcDNA3. 1-Id2, pcDNA3. 1-Id2-DBM and pcDNA3. 1-Id2-DBM-δHLH vectors. The downregulation of E-cadherin protein was accompanied with increased migration capability in pcDNA3. 1-Id2-DBM and pcDNA3. 1-Id2-DBM-δHLH vectors-transfected cells. Conclusion: Overexpression of Id2 promotes the migration capability of SKOV3 cells, which might be related with the downregulation of E-cadherin. The action still exists after the HLH domain deletion in Id2 gene.

Objective To illustrate the relationship between the serum viral load, clinical types and the G1896A in precore, A1762T/G1764A in basal core promoter (BCP) or united mutation of them of HBV from patients with HBeAg-negative hepatitis. Methods Sera from 240 HBeAg-negative, 60 HBeAg-positive patients, and 40 negative controls were collected. Mutations of G1896A and A1762T/G1764A were detected using CD-PCR. Viral load in sera was demonstrated using real-time quantification PCR. Results The mutations of G1896A were detected in 57.6% and 6.7% in HBeAg-negative and HBeAg-positive patients respectively. The mutations of A1762T/G1764A were detected in 37.9% and 31.7% in the same patients respectively. The united mutations were detected in 13.5% of HBeAg-negative patients. G1896A was associated with low viral load, and A1762T/G1764A did not have any special relation viral load in sera from HBeAg-negative patients. The united mutations often occurred in patients with severe chronic hepatitis B, and been not related to the viral load. Conclusions The replication capability of G1896A variant decreased without HBeAg production as usual. For A1762T/G1764A variant, the situation of its replication activity was complicated. Its HBeAg production was less influenced than that of G1896A variant. The united variant might has higher pathogenicity and replication activity, and to which much attention should be paid in the future.