MicroRNA (miRNA) regulate the expression of target mRNA according to the specific base pairing. They are involved in proliferation,differentiation and apoptosis of cells. The expression of miRNA has distinct differences between normal tissues and cancers, miRNA can be oncogenes but also suppressor genes, they play an important role in cancer. And miRNA are promising to be a new therapeutic target. In this review, we will discuss the advance in the research of miRNA in cancer.

Objective: To observe the clinical effects of needling acupoints Shenting(GV 24),Neiguan(PC 6) and Sanyinjiao(SP 6) in the treatment of migraine. Methods: Thirty cases of Germen patients with migraine were treated mainly by Shenting(GV 24), bilateral Neiguan(PC 6) and Sanyinjiao(SP 6), as well as bilateral Hegu(LI 4) and Taichong(LR 3), Touwei(ST 8),Yangbai(GB 14), Cuanzhu(BL 2), Sizhukong(TE 23), Shuaigu(GB 8) and Sibai(ST 2) in the affected side of head, auricular Sympathetic (MA-AH 7), Er Shenmen (MA-TF 1), Heart (MA-IC) and Endocrine (MA-IC 3); another 28 cases were treated by routine acupuncture. The clinical results and immediate pain-relieving effects were observed. Results: The total effective rate was 90.0% in treatment group and 75.0% in control group, χ2= 4.57, P ＜ 0.05; the 24-hour pain-relieving effects were obviously better in treatment group than that in control group, χ2 = 10.11, P＜ 0.01. Conclusion: Needling Shenting(GV 24), Neiguan (PC 6) and Sanyinjiao(SP 6) has better effects than routine acupuncture in the treatment of migraine.

BACKGROUND: Cecropins are a kind of micromolecule protein with antibacterial activity. Eukaryotic cell-expressed or artificially synthesized Cecropins is characterized by low efficiency and high cost. OBJECTIVE: To clone and express an antibacterial peptide gene of Musca domestica Cecropin A, and to identify recombinant expression product. METHODS: Mature Musca domestica Cecropin A encoding nucleotide sequence was searched from the GenBank and amplified by RT-PCR. The gene of Musca domestica Cecropin A was cloned into prokaryotic expression vector pET32a and fused with gene of Thioredoxin (Trx) and expressed in E.coli BL2l (DE3). After induction by isopropyl-β-D-thiogalactoside, the sera of the immunized rabbits were collected after rabbits were immunized with the hemolymph of housefly larvae. Recombinant protein was identified by western blot analysis and N-[Tris(hydroxymethyl)methyl]glycine-sodium dodecylsulfate-polyacrylamide gel electrophoresis.RESULTS AND CONCLUSION: After induction by isopropyl-β-D-thiogalactoside, E.coli BL21 expressed mature Cecropin. Rabbit anti- housefly larvae sera, N-[Tris(hydroxymethyl)methyl]glycine-sodium dodecylsulfate-polyacrylamide gel electrophoresis and western blot analysis results confirmed that expression products were mature Cecropin. These suggest that prokaryotic expression system can be utilized to obtain natural mature Cecropin.

Causality ; Humans ; Occupational Exposure ; Vibration ; adverse effects

Cell Research got all impact factor of 3. 426, which was the highest Chinese SCI journal, but ranked 4th by journal's h index. By analyzing the cites and citations of Cell Research from 1999-2006 using the method of bibliometrics, four main characteristics of Cell Research were found:①The quantity of papers published Oil Cell Research is low and their quality is hish. ②Advantageous opening, with authorship patterns, editorial members and peer reviewers from home and abroad. ③Journal published in English is easy to perform international academic exchange. ④International operation model also played an important role in Cell Research's success.

Epidemiological and clinical studies have provided compelling evidence that suggests a link between the relative lack of infectious diseases and the increase in allergic disorders. Asthma usually starts early in life. The early occurrence of asthma suggests that prinatal influences are of importance.Prenatal or postnatal exposure to endotoxins and other bacterial products may protect against the development of asthma in the infant. We will briefly outline the current state of the relationship of the perinatal infection and asthma in the infant, and focus our review mainly on possible rneehanism of perinatal infection providing protection against asthma in the infant, such as Th1/Th2 immune system, transcription factors, protection by maternal antibodies, endotoxin tolerance, pulmonary inflammation and genetic influences.

Severe acute pancreatitis is a relatively common surgical disease. Patients have a hypermeta-bolic state and nutritional support for patients with SAP is needed. Early enteral nutrition support is a viable option and can improve the nutritional status of patients, protect the gut barrier function, reduce bacteria translocation to regulate the inflammatory response and reduce hospitalization costs. Combined with our do-mestic and foreign literatures in recent years, this review the feasibility and practical implementation of enter-al nutrition in severe acute pancreatitis treatment summarizs.

Objective To investigate the effects of ventilation strategy of permissive hypercapnia on the expression of inflammatory mediators in the patients received laparoscopic radical gastrectomy. Methods Thirty-six patients received laparoscopic radical gastrectomy were randomly divided into two groups: control group and experiment group. The patients in control group received conventional ventilation strategy [partial pressure of carbon dioxide in endexpiratory gas (P<,ErCO2) 34.5-45.0 mm Hg (1 mm Hg =0.133 kPa),pH7.35-7.45], and patients in experiment group received ventilation strategy of permissive hypercapnia(PErCO2 49.5-60.0 mm Hg,pH7.20). Blood samples were collected respectively from the peripheral vein before anesthesia induction (T0), 1 h (T1), 3 h (T2) after operation and when operation finished (T3) for the assessment of interleukin(IL)-8, IL-6,tumor necrosis factor (TNF)- α. Results The levels of IL-8, IL-6, TNF-α at T2, T3 were higher in control group [(96.732±3.173) μg/L and (107.767±3.654) μg/L, (0.273±0.009 ) ng/L and (0.287±0.008) ng/L,(1.443±0.086)μg/L and (1.614±0.107) μg/L] than those in experiment group [(91.771±4.332) μ g/L and (97.682±4.998) μg/L, (0.231±0.008 ) ng/L and (0.241±0.006) ng/L,(1.268±0.066) μg/Land (1.428±0.058) μg/L](P<0.05 or <0.01), mean arterial pressure, partial pressure of oxygen in artery and heart rate were relative stabilization. Conclusions Ventilation strategy of permissive hypercapnia can reduce the released amount of inflammatory mediators and may be advantageous for the patients received laparoscopic radical gastrectomy.

Statins may not only lower cholesterol concentration,reduce the incidence of heart diseases and relieve kidney damage,but also stimulate bone formation obviously,as suggested by a number of observation studies and animal researches.However,the exact mechanism of promoting bone formation has not been very clearly yet.Moreover,their adverse effects and their interaction with other drugs still need further clinical trials.In this review,on the basis of clinical data about the effects of hydroxymethylglutaryl-CoA(HMG-CoA)reductase inhibitors,we summarize current hypotheses on their mechanism and safety,in order to provide reference for the domestic bone reconstruction research.

Objective:To induce experimental autoimmune uveoretinitis in Wistar for investigating the pathogenesis mechanism of uveitis.Methods:18 Wistar rats were immunized by different dosage of bovine soluble antigen(S Ag),and were detected the clinic changes dayly.The animals were anesthetized and killed when they had significant clinic EAU appearance or 3～4 weeks after immunized,the eyes were removed and followed by pathologic studies.Results:The incidence of EAU in the 3 different dosage S Ag immunized rats were 2/12 in 100 ?g S Ag group(6 rats,12 eyes),6/12 in 200 ?g S Ag group(6 rats,12 eyes) and 8/12 in 300 ?g S Ag group(6 rats,12 eyes) separately and the pathology scores were 0?16 76?11.02?17 56?9 96 separately in 3 group of rats.Conclusion:The middling sensitive Wistar rats can be used to induce EAU successly with middling purified S Ag.