Objective:To investigate the role of Danshensu on TGF?1 stimulated signal transduction in rat hepatic stellate cells(HSCs).Methods:The mice HSCs were isolated and cultured for the following study.HSC were treated with different concentration Danshensu(0.062 5～0.250 0 mmol/L)and TGF?1 to detect the proliferation、?-SMA protein expression and the chang of Smad2,Smad3,Smad7 mRNA.Results(:1)Danshensu in the concentration of 0.062 5～0.250 0 mmol/L could prevent the proliferation of HSC induced by TGF?1 in a dose-dependent manner(P

Expression of transferrin receptor was examined by means of an immunohistochemical method and DNA cytometry in tumor cells was assayed by image analysis technique in 32 cases with pleomorphic ade- noma of lacrimal gland.Transferrin receptor was positive in 8.The DNA content of some tumors was increased,the DNA distribution pattern showed two or several peaks,good correlation has been found between DNA distribution pattern and the expression of transferrin receptor,and the DNA distribution pattern of tumors with the expression of transferrin receptor showed mainly two or several peaks.It maybe suggest that expression of transferrin receptor be related to the proliferative nature of the tumor.

The responsiveness of SAC-activated human B cells to human rIL-4 remains controversial. We have proved that rIL-4 definitely supports the proliferation of SAC-activated human tonsillar B cells. B cells preactivated with SAC for 24 hr or 72 hr exhibited the same magnitude of response to rIL-4. However, the 24 hr SAC-B cells showed substantially higher DNA synthesis without supply of rIL-4 than did of 72 hr SAC-B cells. The time that rIL-4 promoted proliferation of B cells is short. The growth factor activity of IL-4 for B cells is strongly unraveled in the first 24 hr cultivation and then, becoming weaker with prolonging of culture period. Two-step assay with 24 hr incubation for each step is introduced for the assessment of rIL-4 activity by SAC-B cells. Anti-? beads activated B cells showed better response to rIL-4 in comparison with SAC-B cells. B cells activated by either anti-? or SAC responded well to rIL-2 or rIL-4.

Objective: To determine the value of CT,DSA,endoscopic ultra sonography(EUS),and a combined protocol for preoperative detection of insulinomas.Methods : We retrospectively studied data from 15 patients with insulinomas who underwent EUS,CT and DSA from 2000 to 2005.All patients were treated surgically.Results: The overall diagnostic sensitivity for helical CT was 36.3%.Endoscopic sonography and DSA showed sensitivity were 72.7% and 80.0% respectively.The overall diagnostic sensitivity for combined helical CT and endoscopic sonography was 72.7%,which was not statistically significant.The sensitivity for combined EUS and DSA was 93.3%,which was statistically significant.Conclusion: EUS is important to detect insulinomas,and the most effective method for revealing insulinomas is a combined imaging protocol that consists of both EUS and DSA.

Objective To investigate the current status of postoperative life quality of 2-to 12-yearold children with congenital heart disease,to compare results between 2-to 4-year-old,5-to 7-year-old and 8-to12-year-old groups.Meanwhile,compare the results with foreign researches.Methods Pedsol cardiac module was adopted to investigate 255 children patients with letters in a certain heart center in Shanghai.The survey included heart problems and treatment,perceived physical appearance,treatment anxiety,cognitive problems and communication.2-to 4-year-old group was reported by parents and others were reported by both children and parents.The results underwent analysis and were compared with data of other countries.Results There existed statistical difference among three groups.Compared with foreign researches,quality of life of Chinese children was lower except heart problems and treatment.Conclusions We should provide suitable health care and family centered nursing according to physical and psychological development of children to improve children's quality of life.

Objective To investigate the effect of cardiopulmonary bypass cardiac surgery on postoperative cognitive function of patients who wre undergoing selective cardiac surgery.Methods Fifty children patients who were scheduled for cardiac surgery were investigated with C-WISC (Chinese revised Wechsler Intelligence Scales for children) preoperative.The children patients were tested at the 7th day and 1 month postoperative by C-WISC and digital span.It could be defined postoperative cognitive dysfunction (POCD) if the C-WISC score decreased 1 SD (standard deviation).Results The incidence of POCD at the 7th day and in the first month postoperative was 40％,and 20％ in pediatric patients who underwent cardiopulmonary bypass surgery.It shows that ascending aorta clip time and cardiopulmonary bypass (CBP)time had negative correlation with intelligence quotient (IQ) (P ＜0.05),but age and education had not any correlation with intelligence quotient in pediatric patients who underwent cardiopulmonary bypass surgery (IQ) (P ＞ 0.05).Conclusions The occurrence of POCD in cardiac surgery children underwent CPB tend to be high,with long time CPB and aortic clip were more likely to have postoperative dysfunction.It shows a close relationship between CPB and aortic clip with POCD.Age and education had no significant influence on postoperative cognitive dysfunction in CPB cardiac surgery children.

Circulating tumor cell ( CTC ) testing is characterized by its invasiveness, sampling convenience and facility for real-time monitoring.The booming detection technologies based on physical or immunological features, as well as the subsequent molecular analysis of CTCs including subtype classification, single cell sequencing and in vitro culture, has greatly enriched our comprehension of CTCs and cancer heterogeneity.Meanwhile, a great of researches have demonstrated the significance of CTCs testing in the clinical practice of cancers involving aspects of early diagnosis, treatment monitoring and prognosis.

Exosome is nanoscale vesicles with bilayer membrane structure, is secreted by multiple living cells in vivo, capable of carrying biological materials such as nucleic acids, proteins and lipids etc.Exosome can protect the information material (such as miRNA), which is easy to be inactivated or degraded in the circulatory system, so that they can be safely transferred to the specific target cells to participate in signal regulation, and play an important role in the information transmission between cells.Exosome is widely found in human blood, urine, pleural effusion and other body fluid samples, and can provide rich, stable, sensitive and specific biological information.As a kind of liquid biopsy specimen, exosome has a very high clinical application value in the future.

Objective To explore the relationship between restoring Gissane angle and prognosis in calcaneal fracture surgery. Methods Forty patients with single side calcaneal fracture (SandersⅡ-Ⅲ), Gissane angle changed more than 15°and having performed open reduction and internal fixation with steel plate were enrolled. In them, 25 patients (experimental group) recovered Gissane angle in surgery referencing the healthy side with X-rays. Another 15 patients (control group) didn't recover Gissane angle. The ratio of calcaneum height and length was measured at 1 week after surgery. At 6 and 12 months after surgery, the function were valued by Maryland score. Results The ratio of calcaneum height and length in experimental group was significantly higher than that in control group:0.60± 0.04 vs. 0.55±0.05, and there was statistical difference (P<0.05). All the patients were followed up. At 6 and 12 months after surgery, the Maryland score in experimental group were significantly higher than those in control group:(88.9± 5.7) scores vs. (80.5±7.3) scores and (89.5 ±5.5) scores vs. (82.5 ±6.4) scores, and there were statistical differences (P<0.05). Conclusions Restoring Gissane angle is benefitial for prognosis. So in calcaneal fracture surgery, the Gissane angle should be recovered as much as possible referencing the healthy side.

As an emergent attack,dyspnea can be caused by a variety of diseases,therefore,it is difficult and important as to the differential diagnosis in clinical practice.In the past,Physicians identified the cause of dyspnea primarily based on the clinical symptoms,signs and laboratory tests.However,dyspnea often misdiagnosed because the similarity and overlapping of clinical manifestations among many different diseases and lack of specificity for those conventional lab tests.Until recently,B-type natriuretic peptide (BNP) has emerged as a highly sensitive and specific index to identify heart failure,and has made it reality that rapid and accurate differential diagnosis of dyspnea in the lab.In the last couple of years,the differential diagnosis of dyspnea has been remarkably improved because of the increasing new test reagents,advanced devices and better assay techniques.The present article will review the laboratory differential diagnosis of dyspnea between cardiovascular and pulmonary diseases.