Objective:To study the expression of Pleiotrophin and Midkine protein in endometrium of endometriosis and its clinical significance.Methods:Expressions of Pleiotrophin and Midkine protein were examined by immunohistochemical staining method in 30 endometrial samples of endometriosis(study group) and 29 endometrium of uterine fibroid(control group).Result:Pleiotrophin protein mainly expressed in the membrane and plasma of glandular epithelium of endometrium.Midkine protein mainly expressed in the plasma of grandular epithelium of endometrium and expressed in nuclei in some samples.The expression of Pleiotrophin and Midkine protein in endometriosis was significantly higher than that in the control group(P

Electromyography ; Humans ; Occupational Health

Objective:To investigate the risk factors of preterm premature ruPture of membrane(PPROM) and their pregnancy outcomes.Methods:The clinical data of 95 cases of patients with PPROM admitted and treated in our hospital from October 2013 to July 2016 were collected,which were divided into two groups,observation group(gestational week 28-33 +6 weeks) and control group(gestational week 34-36 +6 weeks).The risk factors of PPROM,delivery method and pregnancy outcomes were analyzed.Results:Genital tract infection was the main risk factor of PPROM(33.68％).of the incidences of cesarean delivery,neonatal asphyxia,neonatal infections and neonatal death in observation group were significantly higher than those of control group,the differences were statistically significant(P ＜ 0.05).The intrauterine infection and puerperal infection rates of observation group of were higher than those of control group,but differences were not statistically significant (P ＞ 0.05).Conclusions:Reproductive tract infections is the most important risk factor of PPROM.The purpose of active treatment including tocolytic therapy and prevention of infection for the patients with PPROM less than 34 weeks is to prolong the gestational weeks,to reduce the rate of neonatal complication,and to improve the pregnancy outcome.

Objective To learn the prevelance of Acinetobacter baumannii and its drug resistance related with Ⅰ class integron. Methods 22 kinds of antibiotics commonly used in our hospital were used to detect the sensitivity of the clinically separated Acineto-bacter baumannii.Methods PCR was used to detect Ⅰ class integron enzyme gene of Acinetobacter baumannii,and then parts of variable region of Ⅰ class integron enzyme gene were amplified and analyzed for gene sequence.Results The drug resistance of Acinetobacter baumannii phenomenon was very serious,which was multiple drug resistance.The resistance rate of Acinetobacter baumannii to CPZ/SB was 1.2%.And the resistance rates to tigecycline,ofloxacin,imipenem,biapenem,and amikacin were 1 5.4%-69.5%,but the resistance rates to other antibiotics were above 71%.There were 72 of 102 strains including Ⅰ class integron (positive rate was 70.2%).The resistance of strains with Ⅰ class integron was stronger than that of the strains without.The varia-ble area of Ⅰ class integron produced the same enzyme bands when analyzed with double enzyme,suggesting the Acinetobacter bau-mannii was homologous.The gene sequence analysis of Ⅰ class integron showed that the Ⅰ class integron of the Acinetobacter bau-mannii carried kinds of resistant genes such as aacA4,catB8 and aadA13.Conclusion From 2010 to 2013,the Acinetobacter bau-mannii in our hospital is highly resistant to cephalosporins,broad-spectrum penicillin,aminoglycosides and fluoroquinolones and is severely multiple drug resistant.So the antimicrobial drugs for the treatment of infections caused by Acinetobacter baumannii are limited.Ⅰ class integron is closely related to the multiple drug resistance of Acinetobacter baumannii.

Nonunion or delayed union remains one of the major complicatio ns of fractures. Repair of fractures involves a sequence of dynamic events which ultimately restore the integrity of bone and its biomechanical properties, incl uding such methods as surgery, internal fixation, bone grafting, external fixati on, electrical stimulation, ultrasound, and high-energy extracorporeal shock wa ve. The preferred management of nonunion and delayed union is autologous bone gr afting. However, the supply of suitable bone is limited and it often results in additional morbidity to the donor site. Recently some advances have been made in the therapeutic principle for nonunion or delayed union. It is aimed at providi ng non-invasion, less complication, and safe treatment. This article introduces the development of some effective and safe therapies for nonunion or delayed un ion.

In this paper, barcode technique is combined with laboratory information system to improve the degree of automation in laboratory. Barcode stuck to specimen collected, such routine laboratory operations are finished according to barcode as specimen sorting, data transmission, two-way communication of analyzing machine, results verifying, reports inquiring, specimen keeping et al. As the only specimen identification, barcode is used to realize two-way communication between analyzing machine and it, thus laboratory workflow is simplified. The application of barcode technique to clinical laboratory improves work efficiency, result reliability and degree of automation greatly. It is the development direction in laboratory in the future.

FJ, with 9.7 times difference between the highest and the lowest. CONCLUSION: Prepared with different base materials, the permeation ability of of the preparation will be different.

OBJECTIVE:To observe the efficacy of Compound Epidermal Growth Factor Pellicles for recurrent oral ulcer. METHODS: 106 patients with recurrent oral ulcer were randomly assigned to receive Compound Epidermal Growth Factor Pellicles with efficacy-confirmed hospital preparation as control. The clinical efficacy in two groups was compared. RESULTS: The effective rate was significantly higher in patients treated with Compound Epidermal Growth Factor Pellicles than with hospital preparation (control) (89.66% vs. 75.00%,P

OBJECTIVE: To investigate the tagging condition in medication of special population in package inserts in order to give reference for rational drug use of special population. METHODS: 389 pieces of common Chinese and western medicine package inserts of our inpatient and out-patient dispensaries were randomly collected and analyzed statistically in terms of the content of package inserts for special population, including pregnant and lactant women, children and the aged. RESULTS: A part of medication items of special population in package inserts had deficiency. According to the classification of dosage form, the tagging rate of injection exceeded other dosage forms reached to more than 90%, except of medication items for children. The tagging rate of oral formulation arrived at more than 90% only in terms of contraindications and cautions. According to the classification of manufacturer, the tagging rate of domestic drugs was kept at the low level while that of imported drugs exceeded 90%. CONCLUSIONS: Package inserts need to be improved with the effort of drug supervision management department, drug production department and population of using drugs to guarantee the safety of drug use in special population.

OBJECTIVE:To prepare zolmitriptan-diclofenac microemulsion,and conduct quality evaluation and in vitro trans-dermal study. METHODS:Using solubility and microemulsion area in pseudo-ternary phase diagram as indexes,the types of oil phase and mixed emulsifier ratio of zolmitriptan-diclofenac microemulsion were screened;the microemulsion quality was inspected using particle size,Zeta potential,appearance and stability. HPLC was used to measure the contents of zolmitriptan and diclofenac. Transdermal diffusion test instrument was used,2 g microemulsion was smeared in cuticle of extracouporeal rats'skin,and cumula-tive transdermal rate in 24 h was determined. RESULTS:The microemulsion formulation was as follow as 10% oil phase(octanoic acid triglyceride),25% mixture emulgator [polysorbate 80-brij 97 (1:1)],8.3% propylene glycol and 25 mg zolmitriptan,1.25 mg diclofenac,and water adding to 100 mL. The average particle size of prepared microemulsion was(28.2±2.5)nm,Zeta poten-tial was(-3.25±0.33)mV,the appearance was rounding;the microemulsion showed no stratification or flocculation at room tem-perature after placed for 1 month. Contents of zolmitriptan and diclofenac were 0.248 mg/mL,12.46 mg/mL(n=3);24 h cumula-tive transdermal rates were 80%,75%. CONCLUSIONS:Zolmitriptan-diclofenac microemulsion is prepared,and its in vitro trans-dermal ability is good.