Objective:To study the effects of different Epstein-Barr virus (EBV) DNA load, induction chemotherapy+ radiotherapy and concurrent radiochemotherapy on patients with stage Ⅲ nasopharyngeal carcinoma (NPC).Methods:A total of 178 patients with stage Ⅲ NPC were selected as the study subjects in the Department of Otorhinolaryngology of the First People′s Hospital of Xianning of Hubei Province from January 2012 to March 2019, including 44 patients received adjuvant chemotherapy. According to the pre-treatment EBV DNA load of 1 000 copies/ml, the patients were divided into high viral load group (EBV DNA≥1 000 copies/ml, n=53) and low viral load group (EBV DNA<1 000 copies/ml, n=125), and 14 patients in the high viral load group and 30 patients in the low viral load group received adjuvant chemotherapy. According to treatment method, the patients were divided into induction chemotherapy+ radiotherapy group ( n=105) and concurrent radiochemotherapy group ( n=73). The general clinical data, recurrence rate, 5-year overall survival (OS) rate, disease free survival (DFS) rate, local recurrence free survival (LRFS) rate and disease metastasis-free survival (DMFS) rate of each group were compared. Results:Among 178 patients with stage Ⅲ NPC, 34 cases recurred, accounting for 19.10%, and 29 cases died, accounting for 16.29%. There was a statistically significant difference in N staging between the induction chemotherapy+ radiotherapy group and the concurrent radiochemotherapy group ( χ2=6.40, P=0.01). The tumor recurrence rate in the high viral load group was 33.96% (18/53), and that in the low viral load group was 12.80% (16/125), and there was a statistically significant difference ( χ2=10.79, P<0.01). The recurrence rate of lymph nodes [(9.43% (5/53) vs. 1.60% (2/125), χ2=4.15, P=0.04], the distant metastasis rate [18.87% (10/53) vs. 5.60% (7/125), χ2=7.59, P=0.01] were significantly higher than those in the low viral load group, and there were statistically significant differences. The tumor recurrence rate of patients in the induction chemotherapy+ radiotherapy group was 17.14% (18/105), and that in the concurrent radiochemotherapy group was 21.91% (16/73), and there was no statistically significant difference ( χ2=0.63, P=0.43). The 5-year OS rate, DFS rate, LRFS rate and DMFS rate of 178 patients with stage Ⅲ NPC were 84.68%, 72.80%, 79.68% and 79.54%, respectively. The 5-year OS rate (79.25% vs. 92.80%, χ2=6.86, P<0.01), DFS rate (73.58% vs. 88.00%, χ2=5.67, P=0.01), LRFS rate (73.21% vs. 89.24%, χ2=8.32, P<0.01) and DMFS rate (65.24% vs. 78.00%, χ2=4.15, P=0.02) in the high viral load group were significantly lower than those in the low viral load group, and there were statistically significant differences. The 5-year OS rate (89.52% vs. 87.67%, χ2=0.15, P=0.70), DFS rate (84.76% vs. 82.19%, χ2=0.21, P=0.65), LRFS rate (80.38% vs. 79.84%, χ2=0.00, P=1.00) and DMFS rate (79.52% vs. 81.78%, χ2=0.05, P=0.83) in the induction chemotherapy+ radiotherapy group were not statistically significant compared with those in the concurrent radiochemotherapy group, and there were no statistically significant differences. The 5-year OS rate of 44 patients receiving adjuvant chemotherapy was significantly higher than that of patients who did not receive adjuvant chemotherapy (93.77% vs. 87.49%), and there was a statistically significant difference ( χ2=5.21, P=0.02). In the high viral load group, the 5-year OS rate of patients receiving adjuvant chemotherapy was significantly higher than that of patients who did not receive adjuvant chemotherapy (93.77% vs. 84.13%), and there was a statistically significant difference ( χ2=5.11, P=0.03). Conclusion:Induction chemotherapy+ radiotherapy can achieve the same therapeutic effect as concurrent radiochemotherapy. High viral load is associated with high recurrence rate and poor survival rate. For these patients with high viral load, treatment intensity needs to be strengthened.

Objective To investigate the effectiveness of the SF-36 health survey questionnaire used to investigate the quality of life (QOL) of the patients undergoing tibial bone transport.Methods Reliability analysis,correlation analysis and validity analysis were performed to evaluate the reliability and validity of the SF-36 scale.According to treatment time,94 patients who underwent tibial bone transport were divided into 3 groups:group A were 30 patients who had just started bone transport,group B 43 patients who had completed bone transport and removal of external fixation within 2 years,and group C 21 patients who had completed bone transport and removal of external fixation beyond 2 years.A mean comparison of items in the SF-36 scale was further carried out.Results The questionnaires were completed well.The Chonbach α indexes of all the items were more than 0.8,showing good reliability of the scale.Most of the correlation coefficients were almost or more than 0.5,indicating acceptable content validity of the scale.The QOL of the patients undergoing tibial bone transport was differentiated from that of normal people,proving good discriminant validity of the scale.Groups B and C obtained significantly better scores in all items of the scale than group A (P ＜ O.05).Group C scored significantly higher in physical functioning (PF) and role-emotional (RE) than group B (P ＜ 0.05),but there were no significant differences between the 2 groups regarding other items of the scale (P ＞ 0.05).Conclusions With good reliability and validity,the SF-36 health survey questionnaire can be used to assess the QOL of the patients undergoing tibial bone transport.The treatment of bone transport in the tibia can significantly and durably help improve the QOL of the patients.

Objective To mine the relation between leukemia and genes using Weka. Methods The papers on leuke-mia and genes were retrieved from PubMed, their subject headings and subheadings were extracted using BICOMB to generate co-occurrence matrix and term-paper matrix. The research hotspots were found by cluster analysis of the data on co-occurrence matrix using Weka and Cobweb. The literature was verified. Results The 42 high fre-quency words were clustered into 7 classes by Weka. No high frequency words of leukemia or genes were found in classes 1, 2, 4 and 5, indicating that their clustering efficiency was poor. The clustering efficiency of the other 3 classes was good. Conclusion Cluster analysis showed that leukemia is related with myc gene, ab1 gene, p53 gene, virus gene, immunoglobulin gene and mdm gene.

A rapid, sensitive, reliable and simple microdetermination technology for anti-cancer activity, incorporation technology of ~3H-TdR, was developed to seek the process for anti-cancer preparations of compound Chinese medicines. In such a way, more rational process could be drafted during the short time. The preparation made by this process was found to possess good curative effect, no obvious toxicity and no side-effect in the compreshensive evaluation of clinic and a total 14 biology indexes, The application of some rapid and sensitive medical-determination technology in studies of preparation process, quality standards and stability could solve some problems induced by indefinite active components, make research way broad, and raise research level.

Objective To research the association between the prognosis of Acinetobacter baumannii infection and drug resistance among patients in the intensive care unit( ICU). Methods Eighty-four patients with Acine tobacter baumannii infection from February 2003 to April 2009 in Peking University Shenzhen Hospital ICU were retrospectively analyzed and divided into alive group(59 patients) and dead group(25 patients) by using 30-day mortality. The impact of drug resistant Acinetobacter baumannii on mortality was assessed by binary logistic regression. Results The mortality of patients with Acinetobacter baumannii infection was related to age, haematological malignancy, the Chanson Comorbidity Index, APACHEII score, multidrug-resistant Acinetobacter baumannii ( MDRAB) , carbapenem-resistant Acinetobacter baumannii (CRAB ) , septic shock and antimicrobial inappropriate therapy by univariate analysis( P < 0.05). Binary logistic regression revealed that CRAB( OR = 3. 360,95%CI: 1. 563 ～7.223,P< 0.05)and the Charison Comorbidity Index( OR = 1. 633,95% CI: 1. 201 ～2. 221 ,P <0.05) were independent risk factors. Conclusions The prognosis of Acinetobacter baumannii infection was closely correlated with drug resistance. The patients with CRAB infection had a higher mortality.

OBJECTIVE:To explore the treatment effect and complication prevention of various stent materials for intracranial arteriostenosis.METHODS:A computer-based online search of Science Direct and Ei database (1998-01/2009-10) was performed for English articles with the key words of "intracranial arteriostenosis,stent placement" In addition,CNKI and CBM database (1998-01/2009-10) were searched for related Chinese articles with the same key words in Chinese.Moreover,related works were manually searched.Studies regarding various stent materials for intracranial arteriostenosis were included,including basic and clinical experiments.RESULTS:Multicenter studies of worldwide show that stent implantation displays obvious advantages in treating carotid artery stenosis compared with carotid endarterectomy.Recently,with developing of balloon and stent compliance,as well as stent technology,and application of various novel materials and cerebral protection device,stent has become a safe and effective therapy for intracranial arteriostenosis.However,complications following stent implantation,such as restenosis,ischemic stroke,cerebral hemorrhage hypertransfusion syndrome,and cerebral angiospasm,limit its application.CONCLUSION:The safety and efficacy of intracranial stent implantation remains validation of multicenter and perspective studies.High incidence of restenosis following stent implantation requires further improvement.

An analytical method of reversed phase high performance liquid chromatography (RP-HPLC) was developed for simultaneous determination of 5-hydroxymethylfurfural and nine phenolic compounds (including (+)-catechin, (-)-epicatechin, chlorogenic acid, rutin, caffeic acid, protocatechuic acid, syringic acid, ferulic acid and p-coumaric acid) in 20 min.Ten components were detected and separated successfully by Diamonsil C_(18) column (150 mm x4.6 mm, 5 μm) at wavelength of 280 nm and column temperature of 42 ℃, with acetonitrile and 3% acetic acid solution as the mobile phase in gradient elution.The resultant correlation coefficients of the ten compounds were between 0.9911 and 0.9995 with detection limit from 0.2 to 0.5 mg/L, the RSD less than 2.4% and the average recoveries from 89.4% to 98.3%.These experimental results demonstrate that 5-hydroxymethylfurfural and the nine phenolic compounds in different rice wine samples can be determined with the new method for practical uses.

With the emergence of new endocrine drugs and the development of research in resistance mechanisms,significant progress has been achieved in the endocrine treatment of breast cancer in recent years. Fulvestrant,the combination of endocrine drugs and the combination of targeted therapy with endocrine therapy will be effective treatments for postmenopausal women with hormone receptor positive in advanced breast cancer.

Reflux is a common post-gastrectomy complication due to the destruction of physiological antireflux mechanism,which affects patients' post-surgery life quality greatly.It's one of the basic requirements of digestive tract reconstruction to reduce the incidence of post-surgery reflux,and nowadays there are many kinds of anti-reflux surgical methods,which are also hot topics both at home and abroad.The non-surgical anti-reflux treatments are mainly focused on gastrointestinal motility promotion,mucosa protection,acid suppression,and so on.There are still some controversies concerned with which surgical or non-surgical way to choose.It's of great significance to have a deep insight into the associated mechanisms and make a wise decision on the surgical management with proper non-surgical assistance.

Objective To investigate the risk factors of post-pancreaticoduodenectomy complications.Methods The clinical data of 207 patients with pancreatic carcinoma or peri-ampullary carcinoma who underwent pancreaticoduodenectomy at the Anhui Provincial Hospital from Dec.2007 to Dec.2012 were studied.Seventeen clinicopathologic factors to correlate with the postoperative mortality and methods were selected for univariate analysis and multivariate analysis using logistic regression.Results Univariate analysis showed the major risk factors of postoperative mortality and morbidity were a history of previous operation,pre-operative drainage,total serum bilirubin level,alanine aminotransferase level,serum albumin level,serum pre-albumin level,parenchyma texture and pancreatic duct diameter (P＜0.05).Multivariate analysis showed alanine aminotransferase level,parenchyma texture and pancreatic duct diameter were independent risk factors of postoperative complications.Pancreatic duct diameter was the independent risk factor of pancreatic fistula.Pancreatic fistula was the independent risk factor of hemorrhage.Conclusion The postoperative complications of pancreaticoduodenectomy was closely related to alanine aminotransferase level,parenchyma texture and pancreatic duct diameter.