Objective: To observe the clinical effects of needling acupoints Shenting(GV 24),Neiguan(PC 6) and Sanyinjiao(SP 6) in the treatment of migraine. Methods: Thirty cases of Germen patients with migraine were treated mainly by Shenting(GV 24), bilateral Neiguan(PC 6) and Sanyinjiao(SP 6), as well as bilateral Hegu(LI 4) and Taichong(LR 3), Touwei(ST 8),Yangbai(GB 14), Cuanzhu(BL 2), Sizhukong(TE 23), Shuaigu(GB 8) and Sibai(ST 2) in the affected side of head, auricular Sympathetic (MA-AH 7), Er Shenmen (MA-TF 1), Heart (MA-IC) and Endocrine (MA-IC 3); another 28 cases were treated by routine acupuncture. The clinical results and immediate pain-relieving effects were observed. Results: The total effective rate was 90.0% in treatment group and 75.0% in control group, χ2= 4.57, P ＜ 0.05; the 24-hour pain-relieving effects were obviously better in treatment group than that in control group, χ2 = 10.11, P＜ 0.01. Conclusion: Needling Shenting(GV 24), Neiguan (PC 6) and Sanyinjiao(SP 6) has better effects than routine acupuncture in the treatment of migraine.

BACKGROUND: Cecropins are a kind of micromolecule protein with antibacterial activity. Eukaryotic cell-expressed or artificially synthesized Cecropins is characterized by low efficiency and high cost. OBJECTIVE: To clone and express an antibacterial peptide gene of Musca domestica Cecropin A, and to identify recombinant expression product. METHODS: Mature Musca domestica Cecropin A encoding nucleotide sequence was searched from the GenBank and amplified by RT-PCR. The gene of Musca domestica Cecropin A was cloned into prokaryotic expression vector pET32a and fused with gene of Thioredoxin (Trx) and expressed in E.coli BL2l (DE3). After induction by isopropyl-β-D-thiogalactoside, the sera of the immunized rabbits were collected after rabbits were immunized with the hemolymph of housefly larvae. Recombinant protein was identified by western blot analysis and N-[Tris(hydroxymethyl)methyl]glycine-sodium dodecylsulfate-polyacrylamide gel electrophoresis.RESULTS AND CONCLUSION: After induction by isopropyl-β-D-thiogalactoside, E.coli BL21 expressed mature Cecropin. Rabbit anti- housefly larvae sera, N-[Tris(hydroxymethyl)methyl]glycine-sodium dodecylsulfate-polyacrylamide gel electrophoresis and western blot analysis results confirmed that expression products were mature Cecropin. These suggest that prokaryotic expression system can be utilized to obtain natural mature Cecropin.

MicroRNA (miRNA) regulate the expression of target mRNA according to the specific base pairing. They are involved in proliferation,differentiation and apoptosis of cells. The expression of miRNA has distinct differences between normal tissues and cancers, miRNA can be oncogenes but also suppressor genes, they play an important role in cancer. And miRNA are promising to be a new therapeutic target. In this review, we will discuss the advance in the research of miRNA in cancer.

The developed countries have increasingly used private sector involvement in the practices of developing, financing and providing public health infrastructure and services through public-private partnerships (PPPs) in recent years.The main reasons for this uptake in these countries are manifold ranging from the rising expenditures for refurbishing, maintaining and operating public assets, and the increasing constraints on the governmental budgets stifle because of the economic downturn.As a result, the government needs funds to cover inadequate inputs and seeks innovation through private sector experts and management expertise aiming for better operational risks mitigation.In this paper, 18 risk factors in health sector of China were tracked through the literature research, and the research aims to identify the risk allocation by issuing the questionnaires and conducting interviews with experts from the academia, and public and private sectors.This paper finally provides the scheme about the appropriate risk allocation to focus on improving the mitigation of the existing risks in order to achieve successful PPP projects.

Recently, Public-private Partnerships (PPPs) are being increasingly used in the public facilities and services provision in China.The procurement system ranges from simple contracting of services to the involvement of the private sector in the infrastructure financing, design, construction, operation and maintenance.However, organizing a PPP is not an easy task due to its complexity and long term contractual obligations and this some projects to fail to attract the private sector in the partnership and in the services provision.18 critical success factors of PPP project in the Chinese health sector were undermined by the investigation of this research.The mostly identified CSFs are thorough and realistic benefit assessment, sound policies, appropriate risk allocation and risk mitigation, and the public/private sector responsibilities.This paper finally puts forward the recommendations based on the statistics that are published from the Integrated Information Platform of CPPPC in order to focus on validating them for successful PPP projects achievement.

Objective: T o further investigate pathogenesis about vulvovaginal candi diasis (VVC). Methods: Human normal vaginal epithelial cells in vitro were cultured by tissue cultur e and keratinocyto serum-free medium. Passaging epithelial cells were coculture d with candida albicans medium in separate wells for 0, 3, 6, 12, 24, and 48 h. C ontr ol epithelial cells were cultured alone in separate wells. For examination o f cytokines and chemokines, at each time point, the coculture supernatant and th e control culture were collected. ELISA was applied to detect the levels of HBD -1, HBD-2 and SP-A protein expression in the vaginal epithelial cells (HBD-1 :F= 62.784,P=0.001; HBD-2: F=5127.984, P=0.000; F=542.210, P=0 .000). Also, we colle cted the vaginal doughing water of VVC patients and healthy women, and detected the levels of SP-A, then analysed the data. Results: In the tra il of human vagin al epithelial cells, in contrast to the control group, the medium group induced the epithelial cells to secrete more HBD-1, HBD-2 and SP-A at h48, which was co nsidered to be significant (P

Aim To investigate the apoptosis of cervical cancer HeLa cells cultured in vitro transfectead by combining telomerase anti-hTR and anti-hTERT. Methods The activity of telomerase was tested by polymerase chain reaction-telomeric repeat amplification protocolenzyme-linked immunosorbent assay (PCR- TRAP- ELISA). Cell morphologies were observed by fluorescence microscope stained with acridine orange. Apoptosis and cell cycle were examined by Flow Cytometer method (FCM). Results The telomerase activity of HeLa cells transfected by antisense oligonucleotides were significantly decreased compared with those of nontreated control,sense oligonucleotides and oligofectamine TM(P

Objective:To observe whether the in-flow anesthetic gas absorber can reduce the recovery time after stopping inhalation of enflurane or isoflurane. Methods: In fixed tidal volume, minute volume and fresh gas flow, the recovery time of isoflurane or enflurane anesthesia with or without in-flow anesthetic gas absorber were compared after closing the vaporizer.Results: With in-flow anesthetic gas absorber, the time for anesthetic gas concentration in circle to reduce the MAC to 0.3was 3.3±0.5 min for isoflurane, which was significntly shorter than that without absorber (20±0.3min) ( P＜0.01 ) and the time for enflurane was 3.5±0.5min and 25±0.1min respectively. Conclusion:Anesthetic gas absorber can reduce the recovery time of either enflurane or isoflurane inhalational anesthesia significantly.

ObjectivesTo observe correlation between serum vitamin A status and prevalence or severity of NRDS. MethodsRecruited into this study were 166 preterm infants admitted to our neonatal department. The serum concentration of vitamin A was measured, and the prevalence of NRDS at different levels of vitamin A was compared. According to the clinical manifestation and X-rays, there were 30 infants diagnosed as NRDS. Another 30 patients from the rest 133 infants without NRDS were randomly selected as control group according to the gestational ages, then the difference between NRDS group and control group were observed according to gestational ages. The 30 preterm infants with NRDS were divided further into mild group (in-cluding stagesⅠandⅡ,n=18) and severe group (including stageⅢ andⅣ,n=12) according to ifndings chest X-ray, then the difference between these two groups were observed.ResultsIn the 166 preterm infants recruited, 65/166 had serum vitamin A lower than 0.35 μmol/L, in which 14/65 (21.54%) were NRDS patients. Another 93/166 patients had serum vitamin A ranged from 0.35 μmol/L to 0.7 μmol/L, in which 15/93 (16.13%) were NRDS patient. The rest 8/166 had serum vitamin A higher than 0.7 μmol/L, in which 1/8 (12.50%) were NRDS patient. However, the differences between each two groups were not signiifcant. When gestational ages ranged from 34 to 37 weeks, the serum concentrations of vitamin A of NRDS group were lower than that in controls (P<0.05). No signiifcant difference between the serum concentrations of vitamin A and the severity of NRDS were ob-served (P>0.05).ConclusionsThe prevalence of NRDS for those preterm infants lacking of vitamin A was tended to increase. And the late preterm infants (34 w≤ GA <37 w) with vitamin A deifciency are prone to NRDS.

Atrial fibrillation is the most common arrhythmia in clinical practice.However,the therapeutic effects of traditional antiarrhythmic medicines are far from satisfactory,because of the high rate of arrhythmia recurrence and the potential proarrhythmia effect.Recently,with the further investigation of the mechanisms that initiate and perpetuate atrial fibrillation,and the support of extensive data from clinical trials and animal experiments,thera-peutics based on anti-inflammation,antioxidation,regulation on renin-angiotensin-aldosterone system(RAAS)and gap junction may be new therapeutic targets for the treatment of arrhythmia.In this paper,the potential molecular mechanisms involved in these processes and several clinical trials about some non-traditional antiarrhythmic medi-cines were reviewed,in order to propose a new idea about the development of antiarrhythmic medicines.