1.Detection of Chemical Warfare Agent Simulants by UV Photoionization High-Field Asymmetric Ion Mobility Spectrometry
Youjiang LIU ; Chilai CHEN ; Lehua ZHANG ; Xiaotian ZHANG ; Hongwei WANG ; Deyi KONG
Chinese Journal of Analytical Chemistry 2014;(9):1259-1263
Accurate diffusion was used to get low concentrations samples, and then the samples were detected by UV photoionization high-field asymmetric ion Mobility spectrometry ( UV-FAIMS ) . The samples were chemical warfare agent simulants ( CWAS) vapor:dimethyl methylphosphonate ( DMMP ) , dimethyl sulfoxide ( DMSO) , tributyl phosphate ( TBP ) and dimethyl sulfoxide ( DMF) . The results of FAIMS spectra data were analyzed by separation of spectra at different dispersion voltage ( DV ) and compensation voltage ( CV ) . A two-dimensional spectrum of α2 and α4 of CWAS was established. It was shown that FAIMS could identify CWAS well and have a good sensitivity. Take DMMP as a example, the detection limit was better than 0. 55 μg/L.
2.Clinical analysis of 88 cases of lumbar inter-vertebral disc protrusion treated with rapid angulation rotation traction
Lehua YU ; Nanshun WU ; Shiming WU ; Hongying FU ; Xuemei HAN ; Chuanfu ZHANG
Journal of Third Military Medical University 2001;23(4):476-477
Objective To evaluate the therapeutic effect of rapid angulation rotation traction for lumbar intervertebral disc protrusion. Methods A total of 88 patients (66 male and 22 female;age: 18-65), with a history of 2 days to 10 years were analyzed. Among them 20 cases were central protrusion 68 cases were lateral protrusion. Traction range: 60-65 mm, angle: 20°-25°, rotation degree: 20°-25°, traction time: 3 s-1 min. The process of treatment was computer designed. The traction was then 1 to 3 times, with a interval of 4-7 days between two treatments. Results The effective rate was 96% for the case with history <1 month 96% and 85% for cases with history >1 month (P<0.01). The effective rate was 62% for central protrusion, and 87% for lateral protrusion respectively. χ2 test showed the difference was not significant. Conclusion The rapid angulation rotation is an effective treatment for lumbar inter-vertebral disc protrusion and this non operative method should be used as early as possible.
3.Radiotherapy with (186)Re-lipiodol II for primary liver cancer patients.
Lehua SHI ; Zhifeng ZHANG ; Daoling ZHUANG ; Hongyan CHENG ; Yetao GAO ; Mengchao WU
Chinese Journal of Surgery 2002;40(11):814-816
OBJECTIVETo analyze the biodistribution of (186)Re-lipiodol (RL) in patients with primary liver cancer (PLC) after hepatic arterial injection in attempt to assess the potential of RL as a radiopharmaceutical for the treatment of PLC.
METHODSRL was synthesized from (186)Rhenium and Lipiodol by a series of physical and chemical procedures. Doses of RL ranged from 1 110 MBq to 2 220 MBq per patient depending on the volume of tumor. Quantitative gamma camera imaging (ECT) and gamma counting of serum and urine were used to obtain data for dosimetry estimation. Serum tumor marker (AFP) level and shrinkage of tumor were used to evaluate the therapeutic efficacy of RL.
RESULTSIn the hepatic tumor, RL was selectively retained and radioactivity was very high throughout this study. The ratio of tumor concentration to the normal liver tissue concentration (T/NT ratio) was 10 - 14 at 48 hours after injection of RL. The main side effects of this therapy were transient fever and anorexia. No unacceptable toxicity was observed. In 100% of the patients, the therapy resulted in a significant decrease of AFP level and reduction of tumor volume.
CONCLUSIONThe biodistribution and imaging results demonstrated RL localized selectively in tumor, and that RL may be a potential internal radiopharmaceutical agent for the treatment of primary liver cancer.
Adult ; Aged ; Carcinoma, Hepatocellular ; drug therapy ; metabolism ; Drug Carriers ; Humans ; Infusions, Intra-Arterial ; Iodized Oil ; administration & dosage ; Liver Neoplasms ; drug therapy ; metabolism ; Male ; Middle Aged ; Radiopharmaceuticals ; adverse effects ; pharmacokinetics ; therapeutic use ; Radiotherapy Dosage ; Rhenium ; adverse effects ; pharmacokinetics ; therapeutic use ; Treatment Outcome
4.The role of ABCG2 in the sensitivity of glioma to pyropheophorbide-amethyl ester-mediated photodynamic therapy
Li PAN ; Si TIAN ; Li ZHANG ; Haidan LIN ; Hui GOU ; Qing CHEN ; Kaiting LI ; Dingqun BAI ; Yuhan KONG ; Yunsheng OU ; Lehua YU
China Oncology 2017;27(2):81-88
Background and purpose:Adenosine triphosphate-binding cassette superfamily G member 2 (ABCG2), which has been found over-expressed in a variety of cancer cells, takes part in the drug resistance of cancer through effux of anticancer drugs. The purpose of this study was to investigate the mechanisms of human glioblastoma cells sensitivity to pyropheophorbide-a methyl ester (MPPa)-mediated photodynamic therapy (PDT) eradicating tumour cells and its relationship to ABCG2.Methods:U87 and A172 glioma cell lines in the logarithmic growth phase were selected and exposed to the treatment of MPPa-PDT and MPPa-PDT+fumitremorgin C (FTC) respectively. The cell viability was measured with the use of CCK-8 assay. The expression of ABCG2 was detected by Western blot. The intracellular contents of MPPa in each group without illumination were tested by lfow cytometry. Flow cytometry with AnnexinⅤ-FITC/PI double staining was used to detect the cell apoptotic rate. DCFH-DA staining was used to assess the generation of intracellular reactive oxygen species (ROS).Results:The MPPa-mediated PDT could eradicate A172 and U87 cancer cells in an energy-dependent manner. The light energy density in A172 was 8 times of that in U87 when the cell viability reached median lethal dose after MPPa-mediated PDT. The high expression of ABCG2 in A172 cells affected the accumulation of intracellular MPPa. Inhibition of ABCG2, not only could enhance the eradicating effect of MPPa-PDT on A172 cells, but also could increase the yield of ROS triggered by MPPa-PDT and the accumulation of intracellular MPPa.Conclusion:The human glioblastoma cell line A172 is insensitive to MPPa-mediated PDT. The mechanism may relate to ABCG2, which decreases the MPPa content in cancer cells through effux of MPPa, resulting in decline of cytotoxicity.
5.Effect of antipsychotic drugs on life quality of schizophrenic patients: one year follow-up study.
Maosheng FANG ; Lehua LI ; Jingping ZHAO ; Honghui CHEN ; Meng YE ; Xiaofeng GUO ; Zheng LU ; Xueli SUN ; Chuanyue WANG ; Shiping XIE ; Bin HU ; Tiansheng GUO ; Cui MA ; Bo WANG ; Luxian LÜ ; Na LIU ; Hong DENG ; Qi CHEN ; Xiaofang SHANG ; Fajin GONG ; Xiyan ZHANG ; Xiaolin HE ; Jianchu ZHOU ; Yingli ZHANG
Journal of Central South University(Medical Sciences) 2009;34(9):850-855
OBJECTIVE:
To compare the effect of 7 antipsychotic drugs on the life quality of schizophrenia patients including chlorpromazine, sulpiride, clozapine, risperidone, olanzapine, quetiapine, and aripiprazole.
METHODS:
A total of 1,227 stable schizophrenic patients within 5 years onset who took 1 of the 7 study medications as maintenance treatment were followed up for 1 year at 10 China sites. Patients were evaluated by the short form-36 health survey (SF-36) at the baseline and at the end of 1 year.
RESULTS:
The life quality was improved obviously at the end of the follow-up. There was significant difference in body pain, vitality, and mental health (P<0.05) among these antipsychotic drugs.
CONCLUSION
All 7 antipsychotic drugs can improve the life quality of schizophrenia patients. Atypical antipsychotic drugs, especially olazapine and quetiapine, are superior to typical antipsychotic drugs in improving life quality.
Adolescent
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Adult
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Antipsychotic Agents
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therapeutic use
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Benzodiazepines
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therapeutic use
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Dibenzothiazepines
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therapeutic use
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Female
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Follow-Up Studies
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Humans
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Male
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Middle Aged
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Olanzapine
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Quality of Life
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Quetiapine Fumarate
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Schizophrenia
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drug therapy
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Surveys and Questionnaires
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Young Adult