Forty-six cases of scapulohumeral periarthritis were treated by selecting the acupoints far from the affected part plus acupuncture differentiation of symptoms and signs, in comparison with 34 cases treated by simple acupuncture differentiation of symptoms and signs. The result showed that cure occurred in 21 cases,marked effectiveness in 19 cases, improvement in 3 cases and ineffectiveness in 1 case in the treatment group. In control group, cure occurred in 10 cases, marked effectiveness in 15 cases, improvement in 8 cases and ineffectiveness in 3 cases. There was a significant difference in the therapeutic effect between the two groups (P＜0.05).

Iatrogenic vertebral artery injury is a rare complication in cervical spine surgeries,the consequences of which may be catastrophic.With the extensive development of cervical spine surgeries and application of diverse new internal fixations of cervical spine,there is a potential increase of risk of iatrogenic vertebral artery injury,which should be paid attention to.This article reviews the rate,cause and management etc.of iarogenic vertebral artery injury.

Objective To review our clinical experiences in the treatment of big area burn accompanying in-halation injury to explore the optimal preventive measures and therapy. Methods To analyze the clinical cases con-ducted of 20 such admitted the big area burn aeeornpanying inhalation injury from 2004 to 2007. Results 18 patients (90%) were cured and 2 patients(2%) died. Conclusion The curative rate of big area burn accompanied by inha-lation injury can be enhanced by combining various treatments,including early treat,early presentive tracheotomy,ap-propriate tracheobronchial lavage,and clear traeheoobronchial secretion.

Objective To evaluate the effectiveness and feasibility of web-based lifestyle Or behavior intervention on Chinese urban adults.Methods Six hundred and eighty-one adult subjects were rand omly assigned to the intervention group (n=341) or the control group (n=340).The intervention group was encouraged to visit a specified interactive web site at 1east once a month to submit self-report health diaries.and provided with individualized health promotion instructions and tailored counseling at the chat room or through email.The control group received routine medical services.The primary outcomes were changes in cigarette smoking,alcohol consumption,fruit or vegetable intake,and physical exercise duration at 6 month from baseline.Intention-to-treat method was used for data analysis.Results At 6 month,smoking cessation rate was 23.6% in intervention group and 4.6% in control group (X2=22.4,P<0.05),and daily alcohol consumption decreased by 28.6 g in intervention group compared to 6.1 g in control group (t=14.9.P<0.05).There Was no significant change in physical exercise duration for those with sub-optimal physical activities between the two groups.In contrast to the control group.mean systolic blood pressure,fasting blood glucose,and body mass index of the hypertensive,diabetic,or overweight/obese subjects in the intervention group showed statistically significant decrease.There was no significant change in serum cholesterol level for the participants with hypercholestemlemia in both groups.The attrition rate was 6.7% in the intervention group.Conclusion Web-based health management services may be helpful in promoting healthy lifestyle and behavior and enhancing the care of chronic conditions including hypertension,diabetes,overweight and obese.

Objective: To investigate the effect ofintestinal trefoil factor (ITF) on the transcriptional activity of ITF promoter and to explore the regulatory mechanismofJanus kinase/signal transducersand activators of transcription(JAK/STAT) on ITF promoter. Methods: The 5' flanking sequence of the ITF gene was cloned from human whole blood genomic DNA by PCR. ITF promoter fragment was cloned and inserted into the pGL3-Basic vector to construct recombinant vector. ITF promoter vector was stimulated with ITF at various concentrations and the luciferase activity was measured. The JAK-STAT3 signal transductionpathway was then blocked by a speciifc inhibitor AG490 to determine the signal pathway involved in ITF promoter activity. Results: Restriction endonuclease analysis and DNA sequencing confirmed that the recombinant plasmid, containing ITF promoter, was constructed successfully. After transient transfection, the activity of ITF promoter was increased signiifcantly in the presence of ITF (P<0.05). Blockage of the JAK-STAT3 signal transduction pathway with AG490 signiifcantly reduced the ITF promoter activity (P<0.05). Conclusion: ITF increases the transcriptional activity of ITF promoter via the JAK-STAT3 signal transduction pathway.

Nitrous oxide/oxygen is one of the inhaled anesthetics that receives attention by scholars at home and abroad for its unique characteristics of excellence comparing with intravenous sedation analgesia which is more commonly used. In addition, the nurses who are the main body personnels of the pain management have certain differences in the implementation of the nitrous oxide/oxygen sedation analgesia both at home and abroad. In this paper, the nitrous oxide/oxygen analgesia and sedation and its nursing implications were reviewed from the physical and chemical properties, pharmacokinetic characteristics, excellent properties, mechanism of action and clinical application, then development directions of management in the future are put forwarded so as to provide theoretical evidences and practical guidelines for domestic medical staffs cooperating to implement the technology.

Objective We observe the ?-aminobutyric acid(GABA)′s variation in the visual cortex of Royal College of Surgeons Rat(RCS rat)′s retina following the photoreceptor cell transplantation. Method The healthy Wistar rats′photoreceptor cells were grafted into the sub-retinal space of the left eye of the RCS rat by outer-path way.Two weeks later,host animals were sacrificed.Both sides of visual cortex were processed for light microscopic analysis.So did the 74 days Wistar rats and 74 days RCS rats.Then immunocytochemistry was used to determine the localization of the amino acid neurotransmitters(?-aminobutyrate,GABA).Results After two weeks of transplantation,the transplanted photoreceptor cells survived.On the opposite side of the visula cortex in the grafted RCS rats,GABA-like immunoreactivity was intensely stained.But the same side of the visual cortex in the grafted RCS rats,GABA-like immunoreactivity could not be observed.Conclusion These results suggest that we can rebuild the visual pathway by transplanting the photoreceptor cells.In the visual cortex of the grafted RCS rats, GABA- like immunoreactivity may indicate that the visual cortex had reflection to the stimulation after photoreceptor cells had been grafted.

The hotspots and frontiers in studies on medical information education were identified with key words cluster algorithm and burst words detection algorithm by making use of CiteSpaceⅢwith 540 papers on medical in-formation education published in core journals of medical information education from 2007 to 2016 as sample data, which showed that the 7 hotspots and frontiers in studies on medical information education from 2007 to 2016 in medical information education field of China and can thus provide support for keeping abreast of its development trend and working out its development strategies.

To study the chemical constituents from the bark of Myrica rubra, fourteen compounds were isolated from the methanolic extract using various chromatographic techniques, including silica gel, Sephadex LH-20 and preparative HPLC. Their structures were identified on the basis of chemical properties and spectroscopic data, as 3, 5-dimethoxy-4-hydroxymyricanol (1), myricanol (2), myricanone (3), myricanol 11-sulfate (4), myricitrin (5), quercetin (6), quercetin-3-rhamnoside (7), tamarixol (8), uvaol (9), ursolic acid (10), taraxerol (11), myricadiol (12), β-sitosterol (13) and β-daucosterol (14). Among them, compound 1 is a new compound, named as 3, 5-dimethoxy-4-hydroxymyricanol, compounds 8, 9 were isolated from the genus Myrica for the first time.
Diarylheptanoids ; chemistry ; isolation & purification ; Myrica ; chemistry ; Phytochemicals ; chemistry ; isolation & purification ; Plant Bark ; chemistry

Aim: Clone and characterize of the 5′- flanking region of the nitrate reductase (NR) gene derived from Dunaliella salina(D. salina). Methods : The genomic DNA from D. salina was respectively digested with BamHI, EcoRI, HindIII, Pst I, Sal I and Xba I. A genomic walking cassette was ligated to the ends of the digested DNA fragments, and then genomic walking libraries comprising BL, EL, HL, PL, SL and XL were constsucted. The 5′- flanking region of the NR gene from genomic walking libraries of D. salina was amplified by LA-PCR. The DNA sequences were analyzed with the software - Promoter Predictions. Isolated 5′-flanking regions fused to the GUS gene were tested for transient expression in the alga. Results: A single specific PCR product of about 1200bp in length from the HL library was generated. Also, several conserved motifs, such as CAAT-box, GAGA-box were found, which are related to regulation of transcription, and the putative binding sites of transcriptional factors such as EBP, EFII, NF-E1 and LV. BLAST showed that the DNA sequences shared high homology with 5′-upstream region of the NR gene from Dunaliella viridis. The isolated 5′-flanking regions were able to strongly drive GUS reporter gene expression, suggesting that it contains the promoter elements necessary for the transcription of the NR gene. The expression pattern of the GUS gene and the NR gene were similar, both ware induced by nitrate and repressed by ammonium. Conclusion: The cloned 5′- flanking sequences of NR gene derived from D. salina might be a specific promoter with the ability to“switch on or off” an expression of the heterologous gene in transgenic D. salina.