Objective To examine the effect of lutein supplementation on visual function in people with long-term display light exposure. Method Thirty-seven persons (age 22-30) with long-term display light exposure were divided into low-dose group, high-dose group, and placebo group involving supplementation with 6 mg lutein/d, 12 mg lutein/d, and placebo for 12 w. The levels of serum lutein and visual function indices such as break-up time (BUT), critical flicker fusion frequency (CFF), visuognosis persistence, and reaction time were measured at week 0 and 12. Results After lutein supplementation serum concentrations of high-dose group and low-dose group increased from (0.328?0.120) ?mol/L to (0.733?0.354) ?mol/L, and from (0.356?0.117) ?mol/L to (0.607?0.176) ?mol/L respectively. There was significant increase of break up time in left eyes of high-dose group. Visuognosis persistence was significantly improved, and significant decrease of simple reaction time was observed in high-dose group. No statistical change was observed in critical flicker fusion frequency, discriminative reaction time and choice reaction time during the study. Conclusion Visual function indices such as break-up time, visuognosis persistence and simple reaction time in people with long-term display light exposure who received lutein supplements were significantly improved.

Endothelial Cells ; pathology ; Endothelium, Vascular ; cytology ; pathology ; Humans ; Mucocutaneous Lymph Node Syndrome ; pathology

Objective To compare the efficacy and safety of glargine(Lantus) versus biphasic insulin aspart 30 (30% free and 70% protamine-bound, BIAsp 30) after continuous subcutaneous insulin infusion treatment (CSII) in newly diagnosed type 2 diabetes mellitus. Methods A 20 week open and random study was performed. All 60 patients with newly diagnosed T2DM were randomly divided into two groups. Group B patients was treated by glargine and group A received treatment with BIAsp 30 administered immediately before dinner and breakfast. Blood glucose at 7 time points, glycosylated hemoglobin A1c(HbA1c) and hypoglycemia were observed. Results The postprandial glucose(PPG) was significantly lower in group B than in group A (P<0.05), and the frequency of hypoglycaemic episodes was lower in group B than in group A (P<0.05). The fasting blood glucose(FBG) was not different between two groups (P>0.05). And there was no difference between two groups in HbA1c and other adverse events. Conclusions Glargine is surperior to BIAsp30, not only in controlling the PPG but also in reducing the incidence of hypoglycemia.

Objective:To evaluate the efficacy of Liangxue-Xiaofeng Decoction combined with acitretin A capsule in the treatment of psoriasis vulgaris. Methods:A total of 80 patients with psoriasis vulgaris from March 2015 to June 2019 in our hospital, who met the inclusion criteria, were divided into two groups according to the random number table method, 40 patients in each group. The control group was given acitretin A capsules on the basis of conventional western medicine treatment, and the study group was given Liangxue-Xiaofeng Decoction on the basis of the control group. Both groups were treated for 2 months and followed up for 6 months. The skin lesion area and Psoriasis Area and Severity Index (PASI) scores were performed before and after treatment. The levels of IL-8, IL-6 and TNF-α were detected by real time PCR amplification, and the levels of histone acetyltransferase (HATs) and histone deacetylase (HDACs) by ELISA. The adverse reactions and recurrence during the follow-up period were recorded and the clinical efficacywas evaluated. Results:The total effective rate of the study group was 82.5% (33/40) and that of the control group was 62.5% (25/40). The difference between the two groups was statistically significant ( χ2=4.012, P=0.045). After treatment, the PASI score of the study group was significantly lower than that of the control group ( t=7.921, P<0.01), and the serum levels of IL-8, IL-6 and TNF-α were significantly lower than those in the control group ( t=12.749, 13.933, 10.856, all Ps<0.01); the activity of HATs [(35.03 ± 3.79) g/L vs. (29.26 ± 2.41) g/L, t=8.125] of the study group was significantly higher than that of the control group ( P<0.01), and HDACs activity [(31.00 ± 2.71) g/L vs. (37.55 ± 3.08) g/L, t=10.098] of the study group was significantly lower than that of the control group ( P<0.01). Conclusions:Acitretin A capsule combined with Liangxue-Xiaofeng Decoction can reduce the level of inflammatory cytokines in patients with psoriasis vulgaris, and prevent the disease progression. The mechanism may be related to the regulation of homeostasis balance in acetylation.

Objective To studytherolesof hypoxia-inducible factor-lalpha( HIF-1α )and vascular endothelial growth factor ( VEGF) in the pathogenesis of hypoxia-induced pulmonary hypertension ( HPH) in neonatal rats.Methods Wistar neonatal rats were assigned into HPH group and the control group using random number table method. Neonatal rats in HPH group were exposed to hypoxia according to HPH model. On day 3 , 7 ,14 and 210f hypoxia,the mean pulmonary artery pressure ( mPAP) , the level of mRNA and protein expression of HIF-1α and VEGF in lung tissue were examined using RT-PCR and Western blot methods respectively. The correlation of HIF-1,VEGF and mPAP were also analyzed. Results The mPAP ( mmHg)in HPH group on day 3 ,7 ,14 and 210f hypoxia were all higher than the control up[8.5±1.5)vs.5.2±1.0),(12.1±2.1)vs.9.6±0.8),(12.9±2.0)vs.(9.1±0.8),(21.0±2. 3) vs. (11. 2 + 1. 6) ,P < 0. 05]. 0n day 3, 7 and 140f hypoxia,the mRNA of HIF-1α in lung tissue of HPH group were significantly higher than the control group ( P <0. 05 ) . On day 70f hypoxia, the HIF-1αprotein in lung tissue of HPH group was significantly higher than the control group(P <0. 05 ) . On day 7 ,14 and 210f hypoxia,the mRNA and protein of VEGF in lung tissue of HPH group were also significantly higher than the control group( P <0. 05 ). Correlation analysis showed that HIF-1α protein were positively correlated with mPAP on day 3 ,7 . 14 and 210f hypoxia in HPH group ( r = 0. 504 .P = 0. 002) , and VEGF protein were positively correlated with mPAP in HPH group on day 7 , 14 and 210f hypoxia( r = 0. 782, P < 0. 001) .Conclusion Both HIF-1α and VEGF play roles in the occurrence and development of HPH in neonatal rats.

Objective To explore the impact of a“one-week”staged multivessel percutaneous coronary intervention (PCI) versus culprit-only PCI on deaths and major adverse cardiac events (MACE). Methods We retrospectively analyzed 447 patients with multivessel disease who experienced a ST-segment elevation myocardial infarction (STEMI) within 12 h before undergoing PCI between July 26, 2008 and Septem-ber 25, 2011. After completion of PCI in the infarct artery, 201 patients still in the hospital agreed to undergo PCI in non-infarct arteries with more than 70%stenosis for a“one-week”staged multivessel PCI. A total of 246 patients only received intervention for the culprit vessel. Follow-up ended on September 9, 2014. This study examined the differences in deaths from any cause (i.e., cardiac and noncardiac) and MACE between the two treatment groups. Results Compared to a culprit-only PCI treatment approach, the“one-week”staged multivessel PCI was strongly associated with greater benefits for 55-month all cause death [41 (16.7%) vs.13 (6.5%), P=0.004] and MACE [82 (33.3%) vs. 40 (19.9%), P=0.002] rates. In addition, there were significant differences in the number of myocardial infarctions [43 (17.5%) vs. 20 (10.0%), P=0.023], coronary-artery bypass grafting [CABG;20 (8.1%) vs. 6 (3.0%), P=0.021], and PCI [31 (12.6%) vs. 12 (6.0%), P=0.018]. Patients undergoing culprit-only PCI compared to“one-week”PCI had the same number of stent thrombosis events [7 (2.8%) vs. 3 (1.5%), P=0.522]. Conclusions Compared to a culprit-only PCI treatment approach,“one-week”staged multi-vessel PCI was a safe and effective selection for STEMI and multi-vessel PCI.

Calcaneus ; diagnostic imaging ; Female ; Humans ; Radiography ; Tuberculosis, Osteoarticular ; diagnosis ; diagnostic imaging ; Young Adult

Objective To compare bone marrow and lymph node biopsy specimens of two kinds of multidrug resistance (MDR) proteins expression and clinical significance in untreated non-Hodgkin lymphoma.Methods We developed multiparameter flow cytometric assay to quantify expression of these multidrug resistance (MDR) proteins in lymph node viable blasts and reverse transcriptase-polymerase chain reaction (RT-PCR) method to semi-quantitate mdrl mRNA of non-Hodgkin lymphomas, including 41 lymph node and 37 bone marrow, and further study of the correlation of the multidrug resistance proteins with general clinical parameters and treatment outcome. Results In two kinds of samples P-gp, MRP and LRP over-expression of three kinds of drug-resistant gene and frequencies are very low. Patients with P-gp expression had a poor outcome of chemotherapy than those without P-gp (P ＜0.05), Patients with LRP expression had poor outcome of chemotherapy than those without LRP (P ＜0.01). In the lymph node, P-gp expression was associated with higher clinical stage (P = 0.046), elevated serum lactate dehydrogenase levels (P ＜0.05). MRP wasn' t associated with higher clinical stage (P =0.369), elevated LDH (P =0.762) and higher malignant degree(P =0.214). LRP expression was associated with higher clinical stage, elevated LDH and higher malignant degree (P ＜0.05). And in the bone marrow, LRP expression was associated with elevated LDH (P =0.003), but the others have no differences. Conclusion These data suggest that P-gp and LRP expression are important mechanism of drug resistance associated with worse clinical outcome in previously untreated NHL. The use oflymph node biopsy to detect drug-resistant protein can provide more accurate clinical information than that of bone marrow.

A novel peptide, named BF2-X, was designed based on the structure-activity analysis of an analogue of Buforin II, named BF2-A. The BF2-X was a hybrid peptide containing the N-terminal residues 5 to 13 of BF2-A and three repeats of the C-terminal regular alpha-helical motif RLLR, and the residues 8 valine were replaced by leucine. The results of bioinformatics analysis had showed that compared with BF2-A, the helicity, positive charge, hydrophobicity rate and C-terminal amphipathy of BF2-X had remarkably enhanced. Both peptides showed a random coil structure in an aqueous solution, while displaying a typical alpha-helical structure in 50% trifluoroethanol solution (a membrane mimic condition). BF2-X exhibited higher alpha-helical contents than BF2-A in hydrophobic environment. BF2-X displayed potent antimicrobial activities against a broad spectrum of microorganisms. And BF2-X showed stronger antimicrobial activities against bacteria tested than parent peptide BF2-A. These results suggest that the alpha-helical content was directly correlated with the enhanced antibacterial activity. Both peptides had no hemolytic action on mouse erythrocyte.

Objective To understand the vision functioning related quality of life in rural residents of Shanxi province,and explore its related factors.Methods By using a multi-stage stratified cluster sampling method,237 rural residents from four township of Shanxi Province were conducted a questionnaire survey of vision functioning related quality of life.Using single factor and multiple factor regression model,the factors related to vision functioning related quality of life were explored.Results There were effective survey of 223 people,including 65 males (29.10％) and 158 females (70.9％).The age of residents was (35.8 ±14.2) years old.As the three aspects of vision functioning related quality of life (general health and vision acuity,grades of activity disabilities,reflections of vision problems) scores were 70.87 ± 17.19,91.56 ±9.13,85.20 ± 19.73 respectively.The visual functioning of general health and visual acuity scores and ophthalmic use and ocular history was negatively correlated; grades of activity disabilities scores and family history and history of drug was negatively correlated; reflections of vision problems scores were negatively correlated with age,but positivley related with mental state.Conclusion Vision functioning related quality of life is related with multiple factors,including age,ophthalmic use,ocular history,medication history,family history of ocular disease,and state of mind can affect vision functioning related quality of life score.Health education need strengthen to the residents according to the actual situation.