Objective: To evaluate the risk of depressive disorders using memory task indicators, so as to provide insights into clinical assessment of depressive disorders. Methods: A total of 68 patients with depressive disorders undergoing treatments in the departments of psychiatrics and clinical psychology in a tertiary hospital during the period from January to September, 2021 were enrolled as the case group, while a total of 31 hospital employees, social volunteers and university students served as controls. The error rate and response time of classical memory task experiments were compared between the two groups, including implicit memory, short-term memory and working memory tasks. In addition, the predictive indicators of depressive disorders were identified using multivariable logistic regression analysis and receiver operative characteristics (ROC) curve. Results: The case group included 29 men and 39 women and had a mean age of (24.12±7.40) years, including 46 subjects with an educational level higher than diploma. The control group included 15 men and 16 women and had a mean age of (26.45±6.65) years, including 23 subjects with an educational level higher than diploma. Multivariable logistic regression analysis showed significant associations of age of >18 years (OR=3.431, 95%CI: 1.259-9.350), error rate of 2-back task (OR=1.056, 95%CI: 1.016-1.097) and error rate of short-term memory tasks (OR=1.078, 95%CI: 1.009-1.152) with the development of depressive disorders. ROC curve analysis showed that the error rate of 2-back tasks showed an area under the ROC curve (AUC) of 0.730 (95%CI: 0.630-0.831), cutoff of 22.5%, sensitivity of 42.6% and specificity of 93.5% for prediction of the risk of depressive disorders, and the error rate of short-term memory tasks showed an AUC of 0.717 (95%CI: 0.605-0.829), cutoff of 23.5%, sensitivity of 67.6% and specificity of 71.0% for prediction of the risk of depressive disorders. In addition, the combination of the error rate of 2-back tasks and the error rate of short-term memory tasks showed an AUC of 0.829 (95%CI: 0.734-0.923), sensitivity of 75.0% and specificity of 80.6% for prediction of the risk of depressive disorders. Conclusion Short-term and working memory task indicators are feasible for assessment of the risk of depressive disorders.

Objective: To analyze the working memory impairment in patients with different degrees of depressive disorder and its influencing factors, so as to provide the basis for the clinical evaluation of cognitive function in patients with depressive disorder. Methods: From January 2019 to November 2020, the patients with depressive disorder were selected in the psychiatry and clinical psychology departments of a tertiary hospital. Demographic information, disease course and medication were collected. Depression subscale ( DEP ) of Mental Health Inventory ( PHI ) was used to measure the degree of depressive disorder. N-back tasks were used to test working memory, with error rate and reaction time as experimental variables to compare the working memory impairment of patients with different degrees of depressive disorder. The multivariate linear regression model was used to analyze the influencing factors for working memory impairment in patients with depressive disorder. Results: A total of 75 patients with depressive disorder were included, including 45 mild cases and 30 moderate and severe cases. The error rates of mild cases and moderate and severe cases in the 3-back task were ( 27.47±20.59 )% and ( 39.60±25.95 )%, the response time between the two groups in 0-back, 1-back, 2-back and 3-back task were ( 458.29±104.19 ) ms and ( 527.90±99.58 )ms,( 499.87±85.46 ) ms and ( 574.57±126.25 ) ms, ( 567.44±114.86 ) ms and ( 675.37±137.54 ) ms, ( 663.76±167.99 ) ms and ( 753.27±152.06 ) ms, the differences between two groups were significant ( P<0.05 ). The multivariate linear regression analysis showed that the severity of depressive disorder was correlated with the response time of 0-back to 3-back tasks and the error rate of 3-back task; whether patients took medicine or not was depressive disorder correlated with the 0-back task response time ( all P<0.05 ). Conclusion There are differences in working memory impairment among patients with different degrees of depressive disorder and the severity of impairment is correlated with the severity of depressive disorder and the use of antidepressant drugs.

ABSTRACT:Objective To investigate the mechanism and induction of T type calcium channel on neural stem cells after brain injury .Methods Adult mice brain injury model was established and divided into control ,sham operation ,surgery ,and surgery+mebefradil groups .Neural stem cells were separated from the subventricular zone (SVZ) and identified .Moreover ,we analyzed the results using MTT and neural stem cell sphere counting after adding different doses of mibefradil in culture medium ,respectively .Then we calculated the half maximal inhibitory concentration (IC50) of mibefradil on neural stem cells .Finally ,we analyzed the expression of Cav3 .2 in SVZ and the protein expressions of Cav3 .2 ,Cyclin A and caspase‐3 in neural stem cells by Western blot .Results In vivo , neural stem cell proliferation was increased in surgery group compared with that in control and sham‐operation groups .The proliferation of neural stem cells in surgery + mibefradil groups was significantly decreased compared with that in surgery group after mibefradil‐induced inhibition of T type calcium channel protein . In vitro , the formation of neural stem cell sphere was significantly inhibited after adding mibefradil .The cell growth ratio was significantly decreased when the concentration of mibefradil was above 5μmol/L .A values in 5 μmol/L ,10 μmol/L and 20μmol/L groups were significantly lower than those in control group (P<0 .05) .IC50 was 8 .93μmol/L .The protein expressions of Cyclin A and Cav3 .2 were inhibited while that of caspase‐3 was increased after mibefradil treatment .Conclusion Neural stem cell proliferation was enhanced by activating T type calcium channel after brain injury .

OBJECTIVETo evaluate the role of serum level of procalcitonin (PCT), C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), and white blood cell count (WBC) as predictors in postoperative early infectious complications with fever after posterior lumbar internal fixation (PLIF).

METHODSA retrospective study was conducted from January 2012 to January 2014. Fifty-two patients with fever in the early stage(within 10 days) after the PLIF were collected in the study. They were divided into infection group and non-infection group (group A and group B) according to the results of postoperative blood culture. There were 26 patients in group A and 32 patients in group B. The values of PCT, CRP, ESR, and WBC count were compared and analyzed between two groups.

RESULTSThe values of PCT, CRP, and ESR in group A were higher than those of group B. Meanwhile, CRP and ESR in group B were still higher than the normal range. Among the 26 patients with infections (group A), PCT was superior to CRP and ESR, had a good ability in discriminating different kinds of postoperative infections. The area under the ROC curve of serum PCT levels was the largest (CI 95% was 0.81 to 0.98) in the indexs; and ROC curve of WBC count was no statistically significant. When the cut off points of each predictors were evaluated, the higher sensitive was CRP and reached at 90.27% and the higher specific was ESR and reached at 88.50%.

CONCLUSIONFor the patients with fever at the early stage after the PLIF should be paid attention and reasonable choosing predictors are helpful to identify postoperative infection in the early stage. The CRP and ESR may be influenced by the surgery, and the PCT level is helpful to differentiate infection type.

Adult ; Aged ; Aged, 80 and over ; Blood Sedimentation ; C-Reactive Protein ; analysis ; Calcitonin ; blood ; Calcitonin Gene-Related Peptide ; Female ; Fever ; blood ; diagnosis ; Fracture Fixation, Internal ; adverse effects ; Humans ; Infection ; blood ; diagnosis ; Leukocyte Count ; Lumbar Vertebrae ; surgery ; Male ; Middle Aged ; Postoperative Complications ; blood ; diagnosis ; Protein Precursors ; blood

BACKGROUND:With the deepening of bone tissue engineering research and bone metabolism understanding, it is a hotspot to analyze the blood supply and nutritional status of tissue-engineered bone.
OBJECTIVE:To compare different methods for evaluating smal vascular network distribution around the knee joint in rats in order to provide a guideline for the study of microvascular network in tissue-engineered bone.
METHODS:Eighteen Sprague-Dawley rats were randomly divided into three groups, with six rats in each group. Three commonly methods were used to evaluate the smal vascular network around the knee joint in rats:immunohistochemistry analysis, angiography analysis, and CT scans and reconstruction analysis.
RESULTS AND CONCLUSION:The microstructure of vascular network could be observed by immunohistochemistry, but the spatial distribution of vessels could not be evaluated. The spatial distribution of vessels could be showed by angiography and CT scans. However, some of micro vessels were showed unclearly by CT scans. The number of blood vessels detected by immunohistochemistry was (26.50±3.02) vessels, significantly higher than those detected by angiography and CT scans that were (14.12±1.47) and (9.00±1.79) vessels, respectively. Combination of immunohistochemistry and angiography can evaluate the microvascular network at microscopic and macroscopic levels, which can provide the whole information of the vascular network.

OBJECTIVETo investigate 3-year antiviral efficacy and side effect of adefovir dipivoxil (ADV) on the old patients with hepatitis B chronic infection.

METHODS31 HBeAg-negative chronic hepatitis B virus infected old patients (include 8 patients with chronic hepatitis B and 23 patients with liver cirrhosis) with serum HBV DNA levels > 1000 copies/ml, and ALT > 2 times the upper limit of normal, without company with other liver diseases, cancer, renal dysfunction, and autoimmune disease. All the patients were treated with ADV orally (10 mg once daily) for 36 months. HBV DNA and biochemical and blood routine indexes were checked after treated.

RESULTSerum total bilirubin, direct bilirubin, alamine aminotransferase, aspartate aminotransferase and load of HBV DNA decrease significantly after therapy (P < 0. 001). Other biochemical indexs and blood routine are no significant changes (P > 0.05).

CONCLUSIONThe way to treat with ADV is safe and effective for old patients with chronic hepatitis B virus infection.

Adenine ; adverse effects ; analogs & derivatives ; therapeutic use ; Aged ; Aged, 80 and over ; Antiviral Agents ; therapeutic use ; Female ; Hepatitis B, Chronic ; drug therapy ; physiopathology ; virology ; Humans ; Male ; Organophosphonates ; adverse effects ; therapeutic use ; Time Factors

Objective In the present study, we investigated the effects of advanced oxidation protein products(AOPP) on reactive oxygen species(ROS) production in murine osteoblastic MC3T3-E1 cells by NADPH oxidase enzymes pathway. Methods Experiments were divided into three groups, including control group, rats albumin(RSA) group, and AOPP group. Different concentrations of AOPP were added to the osteoblastic MC3T3-E1 cells culture medium. The production of ROS in MC3T3-E1 cells was measured by the fluorescence intensity of intracellular fluoroprobe ( DCFD ) . In order to verify the effect of enzyme of the production of ROS, the specific inhibitors of corresponding enzymes were added in the MC3T3-E1 cells which were cultured in the medium with AOPP. Finally, western blot and immunofluorescence were used to observe the changes of NADPH oxidase enzymes subunits. Results Different concentrations of AOPP (50,100,200μg/ml) induced MC3T3-E1 cells to produce different amount of ROS. The higher concentrations of AOPP were added, the more ROS were produced. Furthermore,200μg/ml AOPP induced the maximum amount of ROS production(P<0. 05). Meanwhile, AOPP induced MC3T3-E1 cells to produce different amount of ROS with a time-dependent manner. The peak amount of ROS production in MC3T3-E1 cells was observed in 3h when AOPP were added (P<0. 05). In addition, when specific inhibitors of corresponding enzymes were added in the MC3T3-E1 cells, the production of ROS were significantly suppressed by C-SOD, DPI, and apocynin(P<0. 05). On the other hand, AOPP can up-regulate the expression of Nox4 protein of the MC3T3-E1 cells, which is one of the subunits of NADPH oxidase enzymes. Meanwhile, AOPP can also induce the membrane migration of p47phox subunit. Conclusion AOPP induces osteoblastic MC3T3-E1 cells to produce ROS by NADPH oxidase enzymes pathway, and which may be one of the pathogenesis of AOPP involved in osteoporosis.

Fluorescence intraoperative cholangiography (IOC) is a potential alternative for identifying anatomical variation and preventing iatrogenic bile duct injuries by using the near-infrared probe indocyanine green (ICG).However,the dynamic process and mechanism of fluorescenceIOC have not been elucidated in previous publications.Herein,the optical properties of the complex of ICG and bile,dynamic fluorescence cholangiography and iatrogenic bile duct injuries were investigated.The emission spectrum of ICG in bile peaked at 844 nm and ICG had higher tissue penetration.Extrahepatic bile ducts could fluoresce 2 min after intravenous injection,and the fluorescence intensity reached a peak at 8 min.Inaddition,biliary dynamics were observed owing to ICG excretion from the bile ducts into the duodenum.Quantitative analysis indicated that ICG-guided fluorescence IOC possessed a high signal to noise ratio compared to the surrounding peripheral tissue and the portal vein.Fluorescence IOC was based on rapid uptake of circulating ICG in plasma by hepatic cells,excretion of ICG into the bile and then its interaction with protein molecules in the bile.Moreover,fluorescence IOC was sensitive to detect bile duct ligation and acute bile duct perforation using ICG in rat models.All of the results indicated that fluorescence IOC using ICG is a valid alternative for the cholangiography of extrahepatic bile ducts and has potential for measurement of biliary dynamics.

OBJECTIVEExplore the serum of patients with CHB of HBV large envelope protein (HBV-LHBs) trans-activation function and antiviral therapy effect relationship.

METHODS60 cases of anti-viral treatment of patients with chronic hepatitis B to take every 3 months HBVDNA, HBV-LHBs, as well as detection of hepatitis B immune markers to observe the changes in indexes.

RESULTSIncome group 60 cases of anti-virus group HBVDNA with HBV-LHBs have a higher detection rate of the consistency of the results found no statistical significance (P > 0.05), HBV-LHBs-positive rate and positive rate of HBeAg differences (chi2 = 4.08, P < 0.05). After 24 months of antiviral therapy HBV-LHBs expression always HBVDNA in 29 cases of which occurred 24 months after the negative reaction of the 20 cases, continuous positive were seven cases of non-negative. 60 cases of patients 24 months found no HBsAg seroconversion, four cases of emergence of HBeAg seroconversion.

CONCLUSION(1) detection of serum HBV-LHBs to reflect the hepatitis B virus replication with HBVDNA good correlation. (2) anti-viral treatment of dynamic observation of the process of HBV-LHBs expression can predict the effectiveness of anti-viral therapy.

Adolescent ; Adult ; Antiviral Agents ; therapeutic use ; DNA, Viral ; blood ; genetics ; Female ; Hepatitis B ; blood ; drug therapy ; virology ; Hepatitis B Antigens ; blood ; genetics ; Hepatitis B virus ; drug effects ; genetics ; isolation & purification ; physiology ; Humans ; Male ; Middle Aged ; Treatment Outcome ; Viral Envelope Proteins ; blood ; genetics ; Virus Activation ; drug effects ; Young Adult

OBJECTIVETo discuss the significance of testing hepatitis B virus (HBV) from saliva in HBV patients.

METHODSHBV DNA content in serum and saliva of 200 HBV patients and 20 healthy subjects were detected by fluorescence quantitative polymerase chain reaction. According to the serum level of HBV content, four groups were divided: control group A, group B negative, low virus C (1 x 10(3) - 1 x 10(5) copies/ml) and high-group D ( > 1 x 10(5) copies/ml). The relationship of serum and virus content in saliva was analysed.

RESULTSOf 200 HBV cases, 180 were found HBV DNA in serum with positive rate of 90.0%; while 145 were found HBV DNA in saliva with positive rate of 72.5%, and there was no significant difference (chi2 = 1.35, P > 0.05). The significant difference was observed in testing serum and saliva in Group C (100.0% vs. 38.5%; Z = 14.11, P < 0.01). In group D, there was no significant difference found either (100.0% vs. 83.8%; chi2 = 1.05, P > 0.05). Group D virus serum had a high average level of (6.63 +/- 1.55) log copies/ml virus and in the saliva had an average level of (5.21 +/- 1.85) log copies/ml; saliva had serum viral load lower than an order of magnitude average. No HBV DNA was found in serum or saliva from 20 health subjects.

CONCLUSIONWhen the serum contains a high content of HBV DNA virus, the content of saliva HBV DNA virus should be likely high, which might pose a threat of source of infection. A precise quantitative detection of HBV DNA in saliva might be used as evaluation of the level of virus in the body copy for judgment of infection.

Case-Control Studies ; DNA, Viral ; analysis ; blood ; Female ; Hepatitis B ; diagnosis ; transmission ; Hepatitis B virus ; genetics ; Humans ; Male ; Saliva ; virology