The relative distribution of hepatitis C virus (HCV) genotypes was determined for 123 blood donors. Genotyping was performed using a commercial reverse phase hybridization assay after amplification of the 5’ untranslated region of HCV by the polymerase chain reaction. The isolates comprised, genotype 1a (15.4%), genotype 1b (48.8%), genotype 1 (5.6%), genotype 2 (0.8%), genotype 2a/2c (8%), genotype 4 (0.8%), genotype 6a (14.6%), unusual genotype pattern (2.4%). And serotype hepatitis C virus from genotype 1b was determined
Hepacivirus ; Genotype

Objective To explore the methylation status of hMLH1 and hMSH2 promoter region in the epithelial ovarian cancer and its role in oncogenesis.Methods Methylation status of hMLH1 and hMSH2 promoter region was assayed in 20 normal ovarian tissues,25 benign epithelial tumor,56 malignant epithelial tumor and cell lines SKOV3,3AO by methylation-specific PCR (MSP).SKOV3 and 3AO were analyzed before and after 5-aza-2′-deoxycytidine (5-Aza-CdR) treatment.In addition,an alterations of mRNA expression of hMLH1 and hMSH2 was observed by reverse transcription polymerase chain reaction (PT-PCR).Results No methylation of hMLH1 and hMSH2 promoter was found in normal ovarian tissues. CPG islands methylation of hMLH1 and hMSH2 was observed in 4% (1/25),8% (2/25) respectively in benign epithelial tumor,30.4% (17/56),51.8% (29/56) respectively in malignant epithelial tumor. Methylation status in promoter showed obvious correlation with pathological grade and lymph node metastasis (P

Animals ; Cardiomyopathy, Dilated ; metabolism ; Connexin 43 ; metabolism ; Disease Models, Animal ; Hypericum ; Male ; Plant Extracts ; pharmacology ; Rats ; Rats, Wistar

Objective: To observe the effect effective constituent recombination of Cortex Moutan on thrombosis in virtro and haemorheology in rat. Methods: There were six groups: blangk group, aspirin group(50mg/k), crude drug (1200 mg/k), high dose group (Paeonol 100mg/kg+Paeoniflorin 25mg/kg), mid dose group (Paeonol 50mg/kg+Paeoniflorin 12.5mg/kg), low dose group (Paeonol 25mg/kg+Paeoniflorin 6.25mg/kg). The methods of thrombosis in vitro in rat, arteriovenous shut thrombosis and haemorheology were used to measure the index. Results: In high, middle, low doses groups, the legth,humid weight,dry weight of thrombosis in vitro in rat and thrombosis of arteriovenous shut reduced significantly (P

OBJECTIVETo observe the effect of sodium tanshinone II (A) sulfonate (STS) on Ang II -induced atrial fibroblast collagen synthesis and TGF-beta1 activation.

METHODAtrial fibroblasts of neonatal rats were cultured to determine the content of collagen protein. The original synthesis rate determined by the [3H]-proline incorporation method was taken as the index for myocardial fibrosis. The content of active TGF-beta1 and total TGF-beta1 in cell culture supernatants were tested and cultured by ELISA. The expression of thrombospondin-1 (TSP-1) was assessed by using Western blot.

RESULTAng II could significantly increase the content of atrial fibroblast collagen and the collagen synthesis rate, the TSP-1 expression and the concentration of active TGF-beta1, without any obvious change in total TGF-beta1. After the STS treatment, all of the indexes, apart from total TGF-beta1, were obviously down-regulated.

CONCLUSIONSTS could decrease the secretion of Ang II -induced atrial fibroblast collagen and the synthesis rate. Its mechanism is related to the inhibition of TSP-1/TGF-beta1 pathway.

Angiotensin II ; pharmacology ; Animals ; Collagen ; biosynthesis ; Fibroblasts ; cytology ; drug effects ; metabolism ; Gene Expression Regulation ; drug effects ; Heart Atria ; cytology ; Phenanthrenes ; pharmacology ; Rats ; Rats, Wistar ; Signal Transduction ; drug effects ; Thrombospondin 1 ; metabolism ; Transforming Growth Factor beta1 ; metabolism

Objective To analyse effect of berberine combined with levofloxacin on myocardial enzymes, isozymes and serum inflammatory factors in children with diarrhea.Methods 58 patients who were diagnosed with pediatric infectious diarrhea in our hospital were collected.All patients were randomly divided into experimental group and control group, 29 cases in each group.two groups of patients were given routine treatment, including infusion, maintaining water and electrolyte balance, supplemental calories etc.On the basis of routine treatment, control group was intravenous dripped with levofloxacin injection 200mL,one times per day,and experimental group was treated with berberine hydrochloride tables 0.2 g orally on the basis of control group.The treatment period was one week of two group.After one week of treatment, the serum levels of myocardial enzymes, isozymes, inflammatory factors and clinical curative effect were detected in all patients.Results Compared with control group post-treatment,the serum AST,ALT and LDH levels were lower than that of experimental group(P<0.05);the serum CK and CK-MB levels were lower than that of experimental group(P<0.05);the serum TNF-α,IL-6 and IL-10 levels were lower than that of experimental group(P<0.05);the total effective rate was higher in experimental group(P<0.05).Conclusion The berberine combined with levofloxacin can significantly reduce the serum myocardial enzymes, isozymes and serum inflammatory factors in children with diarrhea, improve clinical curative effect.

Objective To investigate the diagnostic value of prostate specific antigen (PSA) (fPSA, fPSA/tPSA, PSAD) combined with value of apparent diffusion coefficient (ADC) in diffusion weighted imaging (DWI) and magnetic resonance spectroscopy (MRS) in the diagnosis of prostate cancer (PCa) between total PSA (tPSA) 4- 10 μg/L. Methods From December 2013 to 2015 February , 56 elderly male patients with serum tPSA 4-10μg/L were enrolled. Pathological examination confirmed 26 patients with PCa and 30 patients with benign prostatic hyperplasia (BPH). The levels of tPSA and free PSA(fPSA) , fPSA/tPSA, Prostate specific antigen density(PSAD), ADC value according to the DWI, choline(Cho), creatine(Cre) and citrate(Cit) in MRS were analyzed, and ADC, (Cho+Cre)/Cit combined with PSAD and fPSA/tPSA in PCa diagnosis was analyzed by receiver operating characteristic curve (ROC). Results The level of tPSA in PCa group and BPH group had no significant difference (P >0.05), but the levels of fPSA、fPSA/tPSA、prostate volume, PSAD, (Cho+Cre)/Cit, ADC had significant difference:(1.04 ± 0.14) μg/L vs. (1.22 ± 0.34) μg/L, 0.15(0.14- 0.16) vs. 0.17(0.15-0.18), (41.45 ± 5.70) cm3 vs. (48.70 ± 9.97) cm3, 0.17(0.15-0.18) ng/(ml·cm3) vs. 0.16(0.14-0.17) ng/(ml·cm3), 2.22 ± 0.59 vs. 1.17 ± 0.52, 0.98 ± 0.28 vs. 1.39 ± 0.24, P < 0.01 or < 0.05. The area under the curve of (AUC) fPSA/tPSA+PSAD+(Cho+Cre)/Cit、fPSA/tPSA+PSAD+ADC、fPSA/tPSA+PSAD+(Cho+Cre)/Cit+ADC were 0.932, 0.941 and 0.977, and the AUC of fPSA/tPSA+PSAD+(Cho+Cre)/Cit+ADC was the highest. Conclusions PSAD and fPSA/tPSA and the value of ADC in DWI and MRS can be used in diagnosis of PCa.

A novel peptide, named BF2-X, was designed based on the structure-activity analysis of an analogue of Buforin II, named BF2-A. The BF2-X was a hybrid peptide containing the N-terminal residues 5 to 13 of BF2-A and three repeats of the C-terminal regular alpha-helical motif RLLR, and the residues 8 valine were replaced by leucine. The results of bioinformatics analysis had showed that compared with BF2-A, the helicity, positive charge, hydrophobicity rate and C-terminal amphipathy of BF2-X had remarkably enhanced. Both peptides showed a random coil structure in an aqueous solution, while displaying a typical alpha-helical structure in 50% trifluoroethanol solution (a membrane mimic condition). BF2-X exhibited higher alpha-helical contents than BF2-A in hydrophobic environment. BF2-X displayed potent antimicrobial activities against a broad spectrum of microorganisms. And BF2-X showed stronger antimicrobial activities against bacteria tested than parent peptide BF2-A. These results suggest that the alpha-helical content was directly correlated with the enhanced antibacterial activity. Both peptides had no hemolytic action on mouse erythrocyte.

Objective To compare the efficacy of four different methods to locate tracheal tube in the tracheal:modified transillumination method,21/23 cm rule,marked the intubation at a distance,and fiberoptic bronchoscope.Methods A prospective randomized controlled trial was conducted.120 endotracheally intubated adult patients with American Society of Anesthesiologists (ASA) classification Ⅰ-Ⅱ and admitted to Central Hospital of Fengxian in Shanghai from January to March 2015 were enrolled.The patients were randomly divided into four groups (n =30) and located by 21/23 cm rule,marked the intubation at a distance,fiberoptic bronchoscope and modified transillumination method (using homemade locator guided by a red laser fiber to position the depth of tube) respectively.An endotracheal tube was inserted and measured the distance of the tube tip to the carina (TTC),vocal cords to tracheal tube cuff (VC-TC) in three different neck positions,i.e.neck in flexion,neutral,and extension position.The number of improper position in four groups was recorded.Results There were no significant differences in gender,age,and body mass index among the four groups.Six of the 30 patients using marked tracheal tuba method failed to find vocal cords with laryngoscope,while the other three methods all completed successfully.① From neck flexion to extension,TTC was gradually increased,while VC-TC was gradually decreased.In neck flexion and extension positions,TTC distance in the 21/23 cm rule group was significantly shorter than that in the fiberoptic bronchoscope group (cm:1.44 ± 1.14 vs.2.11 ±0.54,3.01 ±1.18 vs.3.80±0.71,both P ＜ 0.05),and the distance was also shorter than that in modified transillumination method group (cm:1.44 ± 1.14 vs.1.93 ± 0.81,3.01--1.18 vs.3.45 ± 0.91,both P ＞ 0.05).VC-TC distance in the 21/23 cm rule group was significantly longer than that in the modified transillumination,the marked intubation,and the fiberoptic bronchoscope groups in neck neutral and extension positions,respectively (cm:3.07 ± 1.08 vs.2.28±0.76,2.29±0.90,2.49±0.86;2.64±0.94 vs.1.82±0.72,1.81-0.94,2.02±0.91,all P ＜ 0.05).TTC and VC-TC distances in three neck positions in the modified transiflumination group were shorter than those in the fiberoptic bronchoscope group without statistical significance.② If TTC was too short,an accidental bronchus intubation could happen,while if VC-TC was too short,an accidental damage of the vocal cord inducing by the cuff press could happen.In the 21/23 cm rule group,there were 7 cases that the tube wrongly inserted to bronchus in neck flexion,and 1 case in neutral and extension positions respectively.In the marked intubation group,there were 4 cases that the tube wrongly inserted into bronchus in neck flexion,and 1 case in neck neutral position,and there were 4 cases that the vocal cords were pressed by the cuffs in extension position.In the modified transillumintion and the fiberoptic bronchoscope groups,there was only 1 case that the tube wrongly inserted to bronchus in neck flexion respectively.Conclusions When neck position changed during trachea intubation,it was easier that the tube wrongly inserted to bronchus for 21/23 cm rule method to locate the position.Bronchus intubations and cuff press vocal cords could happen using the marked tube method,which was less be found using modified transillumination or fiberoptic bronchoscope methods.Finally,the modified transillumination methods can be used to locate with satisfactory effect.