Objective To investigate the value of combined detection by using tumor markers cytokeratin‐19 fragment antigen (CYFRA21‐l) ,neuron‐specific enolase (NSE) ,carcinoembryonic antigen (CEA) and carbohydrate cancer antigen 125(CA125) in lung cancer diagnosis .Methods 200 people who treated in the hospital in related departments from January 2012 to January 2014 were enrolled in the study .100 of them suffered from lung cancer ,53 suffered from benign lung disease ,the rest 47 were healthy people .Electrochemiluminescence method or enzyme‐linked immunosorbent assay(ELISA) were used to detect serum tumor mark‐ers CYFRA21‐1 ,NSE ,CEA ,CA125 and the sensitivity ,specificity and accuracy of different combinations of test items were evalua‐ted .Results In the single detection of those tumor markers for the diagnosis of lung cancer ,CYFRA21‐1 detection got the highest sensitivity and accuracy ,while CA125 got the highest specificity(95 .0% ) .The sensitivity and specificity of combined detection were both significantly higher than single detections .The sensitivity ,pecificity and accuracy of the combined detection of the 4 tumor markers were 92 .0% ,72 .0% ,and 87 .0% respectively .Conclusion In the clinical diagnosis of lung cancer ,the combined detection of several serum tumor markers get higher sensitivity and accuracy than single detection ,which is worthy of clinical promotion .

Cell Growth Processes ; Hemangioma ; pathology ; physiopathology ; Histiocytoma, Benign Fibrous ; pathology ; physiopathology ; Humans ; Neoplasms ; physiopathology

It is documented that nearly half of the global population have been infected with Helicobacter pylori (Hp).In Chinese consensus report on management of Hp infection,bismuth quadruple therapy composed of proton pump inhibitor,bismuth and two antibiotics is recommended as the first-line Hp eradication regimen.However,large doses of oral antibiotics and PPI often disrupt the well-balanced host-microbial symbiotic state,and impair the protective effect of biological barrier.Furthermore,the alteration of microbial composition secondary to eradication therapy might be associated with the increased adverse effects of Hp eradication therapy and re-infection.This paper summarized the advances in study on effects of Hp eradication therapy on intestinal microecology and the advantages of probiotics supplementation in Hp eradication therapy.

Objective To investigate the academic level and influence of the Chinese Journal of Digestive Surgery from the point of articles published and the authors.Methods The articles and authors of Chinese Journal of Digestive Surgery from 2002 to 2012 were retrieved from the Wanfang database and the China National Knowledge Internet database.The full texts were browsed and saved,and then the data were input to Excel for bibliometric analysis.Results There were 1917 articles were published in the Chinese Journal of Digestive Surgery during the past 11 years,and the average number of articles per issue was 29.05.The rate of articles with funded projects was 30.62％,and the citation rate was 58.52％.Authors were from 32 provinces,municipalites and Hong Kong.The numbers of articles contributed by authors from Chongqing,Beijing and Shanghai ranked top 3,which were 446(23.27％),240(12.52％) and 203(10.59％),and followed by authors from Sichuan,Jiangshu and Guandong.The number of articles from affiliated hospitals of Medical Schools was 1382 (72.09％).The cooperation rate was 4.37,and the coauthor rate was 90.03％.Conclusions Chinese Journal of Digestive Surgery is an academic journal with high quality and strong influence.The distribution of authors of the Chinese Journal of Digestive Surgery is wide,and the cooperative rate of authors is high,while the distribution of authors is disproportionate.

Objective Imaging and clinical manifestations of melioidosis were analyzed in order to improve our understanding of the disease and to reduce the rate of misdiagnosis.Methods From 2001 to 2006,12 melioidosis cases were confirmed by blood,pus and sputum culture.All cases were examined with radiography,and nine of them with CT and 2 with US.The imaging and clinical data were assessed retrospectively.Results Ten of 12 cases revealed lung abnormalities,the main organ involved by melioidosis in the body.The appearances were observed as follows:diffused sheets and sheets fused partly in bilateral fields of the lungs can be seen on chest radiograph;homogeneous dense shadow of the lobe or segment were observed on plain chest film and CT;blurred strips radiated from hilum were revealed on plain chest film;multitude nodules in 2 lungs showed on CT;cavity with air and liquid be seen on plain chest film and CT;patches and granules in superior and middle fields or(and)inferior fields be seen on plain chest film and CT;arc shadow of water attenuation in dorsal thorax showed on CT.Infections outside the lung can be observed in orbital,lumbar muscle,liver and spleen in 1 case,and thighbone osteomyelitis with multi abscesses in one case.Conclusion Alhough medioidosis has no characteristic imaging appearances,the disease's location,extent,severity and quantity objectively can be demonstrated by imaging,final confirmation is necessary using the culture of blood,pus and sputum.

Purpose:To investigate the therapeutic effect a nd toxicity of radiotherapy plus ultrasound hyperthermia for treatment of malign ant tumors. Methods:41 cases of locally advanced, recurrent or radioresista nt cancer treated from January 1997 to January 2000 were given radiotherapy plus Sonotherm 1000 ultrasound hyperthermia. The mean radiation dose was 61?12 Gy;h yperthermia was administered within 30 minutes following radiotherapy with mean heating time of (52.4?6) minutes at 1-2 times/week. 3-year local control and survival were estimated by Kaplan-Meier method and Log rank test, factors affec ting prognosis were analyzed by Cox regression. The significance of differences between temperatures was tested by one-way ANOVA. Results:There was significant difference between Tmin and Tmax on tumor local response. Tumor complete response was 58.5%, the 3-year local co ntrol and survival were 13.6%% and 32.69% respectively. There is significant cor relation between radiation dose, tumor volume, Tmin and 3-year local control ( P

Objective To evaluate the sensitivity and specificity of a nested polymersase chain reaction (nested PCR) for detection of the Mycobacterium tuberculosis IS6110 in paraffin embedded tissues. Methods 31 samples from tuberculosis patients and 5 biopsy specimens from patients with hepatitis were subjected to detection of Mycobacterium tuberculosis IS6110 by nested PCR. PCR products of two randomly selected samples were cloned and sequenced. Results Mycobacterium tuberculosis IS6110 was positive in 28 of 31 samples of tuberculosis. The sensitivity and specificity of nested PCR for detection of IS6110 were 90.3% and 100%, respectively. The predictive value of nested PCR was 100%. The sequences of two samples were compared with known sequence of H37Rv isolate (reported by Thierry D) and with nucleotide homology of 97% and 95.3%, respectively. Conclusions Nested PCR is sensitive and specific in the diagnosis of Mycobacterium tuberculosis infection in tissues of routinely paraffin embedded. We propose the diagnostic application of nested PCR for the identification of Mycobacterium tuberculosis infection, especially in the cases which can not be distinguished with certainty from other diseases by histopathology and Ziehl Neelsen's staining.

Objective To investigate the disease causing gene of Stargardt disease. Method Fifteen patients with Stargardt disease were analyzed with 11 primers of the 11 exons of ABCR gene by using PCR SSCP and DNA direct sequencing techniques. Results Three newly detected disease causing mutations were found. Among those mutations, one is a frameshift mutation and others are single base transition. Conclusion This research confirmed that ABCR gene is associated with Stargardt disease, and 3 new mutations of ABCR gene were found.

AIM:Left coronary artery ligation(LAD)was used to induce heart failure.Losartan was microinjected into paraventricular nucleus(PVN),heart rate(HR),blood pressure(BP)and renal sympathetic nerve activity(RSNA)were measured.The mechanism of PVN in chronic heart failure was investigated.METHODS:Sprague-Dawley male rats were selected for LAD ligation for heart failure models,the variation of cardiac function was detected by echocardiography.Losartan was microinjected into PVN,the responses of HR,BP and RSNA were analyzed.RESULTS:Compared with sham group,the level of LV was increased(P