The contents of berberine in Rhizoma Coptidis were determined after desication using different ways including drying in the sun directly or with a thin paper cover, roasted by the low-degree fire and cured in oven at different temperatures. The results show that desicating it in oven at low temperature can preserve the external apperence of Rhizoma Coptidis, cause less damage to the content of berberine, shorten the duration of desication and operate more easily, which is an effective method for processing Rhizoma Coptidis.