The article reviewed the situation of warming kidney and strengthen spleen treatment for ulcerative colitis (UC) in recent 15 years from three aspects: Yang deficiency in spleen and kidney syndrome is one of the most important syndromes in UC; common prescriptions, medicine and efficacy of warming kidney and strengthening spleen treatment for treating UC; the mechanism of the treatment for UC is related to immunity enhancement, inhibition of gastrointestinal smooth muscle, promoting gastrointestinal function, antibiosis, improving plant nerve function, and release of enzymes. It also proposed the problems about warming kidney and strengthening spleen treatment for UC, and prospect for relevant problems.

Objective To analyze the clinical significance of patients self -intervention on self -efficacy and lipid levels in patients with cerebral infarction.Methods 300 patients with cerebral infarction in our hospital were selected from May 2014 to May 2015,and they were randomly divided into two groups by digital table method:the observation group(n =1 50)and the control group(n =1 50),the two groups of patients had no significant differences in the general data.The observation group treated according to the conventional rehabilitation treatments for rehabilitation nursing,in view of the self -management group.After three months,the two groups of patients'self -efficacy and lipid levels were recorded before and after the intervention and the patients'self management abilities were measured. Comparisons between before and after the intervention of the indicators and between the different groups were analyzed,and the relationship of self -management ability and lipid levels of patients was analyzed by Pearson correlation analysis.Results Before the intervention,there was no difference between the two groups in the self -efficacy scale(P >0.05).After the treatment,the self -efficacy scale of the observation group was (29.85 ±5.34), which of the control group was (22.74 ±4.73),the difference between the two groups was statistically significant(t =1 2.207,P <0.001 ).For the lipid levels after treatment,the TC,TG,LDL -C,HDL -C in the observation group were (4.06 ±0.58)mmol/L,(1 .26 ±0.56)mmol/L,(2.27 ±0.49)mmol/L,(1 .42 ±0.54)mmol/L,which in the control group were (4.86 ±1 .05)mmol/L,(1 .54 ±0.48)mmol/L,(3.1 5 ±0.29)mmol/L,(1 .23 ±0.37)mmol/L, the differences between the two groups were statistically significant (t =8.1 68,4.649,1 8.929,3.555,all P <0.001 ).The correlation analysis of self -management ability and the blood lipid improvement of the two groups showed that the observation group presented positive correlation.The patients'self -management ability was stronger, its lipid improve ability was better.Conclusion From the self -efficacy and the blood lipid level analysis,cerebral infarction patients'self -intervention can promote physical and mental health and improve the symptoms of cerebral infarction.In summary,it is helpful for recovery and has positive clinical effect on cerebral infarction.

OBJECTIVE:To assess the ability of suppliers and manufacturers in dealing with drug quality problems so as to provide reference for hospital in optimizing future suppliers and manufacturers.METHODS:The drug quality registration form in drug storehouse in 2007 in our hospital was analyzed in respect of the quality of drugs which were classified into type A and type B.RESULTS:The A-type drug quality problems,i.e drug quality problems presented in the production of drugs, accounted for 28.6%,and B-type drug quality problems,i.e drug quality problems involved in circulation and use of drugs, accounted for 71.4%.CONCLUSIONS:Drug quality data analysis report can objectively reflect the overall condition of hospital drug quality and the drug quality of the suppliers,thus it can be used as reference for hospital in optimizing future drug manufacturing enterprises and suppliers.

BACKGROUND: Emblic leafflower fruit contains rich vitamin C, organic acid and mineral substance. It is indicated in modern research that emblic leaafflower fruit acts on preventing from cancer and anti-aging too. In recent years, more and more concerns have been paid to the researches on functional factor of emblic leafflower fruit and the development of the relevant functional food. It has been verified that emblic leafflower fruit acts on reducing lipid and anti-oxidation in vitro, but there are still lacks of enough basic researches to support it.OBJECTIVE: To probe into mechanism of emblid leafflower fruit on anti-oxidation and protection of vascular endothelial function in rabbits with hyperlipemia.DESIGN: Randomized controlled experimental study based on the experimental animals.SETTING: Atherosclerosis(As) experimental room in a university hospital.MATERIALS: Totally 24 normal NewZealand male rabbits, mass weighted (2.2±0.5) kg.METHODS: The experiment was performed in As Experimental Room of Beijing Anzhen Hospital from September 2001 to May 2002. Totally 24 New Zealand male rabbits were randomized into the control, the power of emblid leafflower fruit group(powder group)(4 g/kg day) and hypercholesterolemia model group (model group), 8 rabbits in each group. In both power and model groups, the rabbits were fed with hypercholesterol food. Oxidase method was applied to assay the content of serum lipid, chemical method to assay the plasma total anti-oxidation and the content of malondialdehyde (MDA), radio-immune method to assay the content of plasma endothelin-1 (ET-1), digoxin labeled probe and tissue hybridism in situ to assay the expression of endothelin mRNA of aortic tunica intima, and image analyzer to assay the area of aortic intimal atherosclerosis plaque and the ratio between the area of tunica intima and the area of tunica media.MAIN OUTCOME MEASURES: The primary outcomes included blood lipid level, serum MDA concentration and the comparison of artery plaque areas. The secondary outcomes included the changes in plasma ET-1.RESULTS: By the comparison between the powder group and model group in triglyceride(TG) and low density lipoprotein cholesterol(LDL-C) were significantly reduced [(11.70 ± 1.73), (14.32 ±2.22) mmol/L, P<0.05; (0.740 ± 0.107), (1.450 ± 0.220) mmol/L, P <0.01 successively]and thehigh density lipoprotein cholesterol(HDL-C) was increased MDA was significantly decreased [ (3.88 ± 0.51 ), (6. 29 ± 1.43 ) mmol / L,P < 0.01 ] and the total anti-oxidation significantly increased [ (10. 771der photo-microscope that the plaque area and the ratio between intima area and media area were remarkably decreased [(39.46±6.53), (50.69± 12.36)mmol/L, P < 0.05; (0. 62 ±0. 32), (1.38 ±0.38) mmol/L, Pgranules of aortic intima ET-1 gene were rem~kably decreased compared with the model group.CONCLUSION: Emblic leafflower fruit prevents from the formation of experimental atheromatous plaque in rabbits probably by regulating rabbit lipid metabolism, improving anti-oxidation to reduce lipid peroxidation and protecting endothelial function to inhibit the expression of artery intima ET-1 gene.

OBJECTIVE: To study the expression of Cyclin D1 in nasopharyngeal carcinoma cells processed by epigallocatechin gallate(EGCG) and it's significance, and revealed the anti-tumor mechanism of EGCG against nasopharyngeal carcinoma. METHOD: CNE-2 cells were treated by EGCG at different concentrations, the morphological changes of CNE-2 cells were observed by inverted microscope; the inhibition ratio of cell proliferation was detected by MTT colorimetric method, flow cytometry was used to analyze the changes of cell cycle. The expression of Cyclin D1 mRNA was detected by RT-PCR. RESULT: After treated by EGCG, the CNE2 cells decreased in amount and density, some of which became roll and small; Floating and dead cells can be seen in the inverted microscopy; cell proliferation was significantly inhibited in a time and dose dependent (P < 0.05). CNE-2 cells were arrested at G1/G0 phase. The expression of Cyclin D1 mRNA was down-regulated by EGCG with concentration and action time dependent (P < 0.05). CONCLUSION EGCG resisted nasopharyngeal carcinoma by inhibiting the cell proliferation, The down regulation of Cyclin D1 mRNA expression in a time and dose dependent may be the possible mechanisms.
Carcinoma ; Catechin ; analogs & derivatives ; pharmacology ; Cell Cycle ; drug effects ; Cell Line, Tumor ; Cell Proliferation ; drug effects ; Cyclin D1 ; metabolism ; Humans ; Nasopharyngeal Carcinoma ; Nasopharyngeal Neoplasms ; metabolism ; pathology

OBJECTIVE To investigate theradiosensitization of combined inhibition of signal transducer and activator of transcription 3(STAT3) and hypoxia-inducible factor-1α(HIF-1α)on laryngeal squamous carcinoma of xenograft mice and its underlying mechanisms.METHODS Xenograft mice were divided into 5 groups randomly: control group(A), irradiation group(B), irradiation and AG490 group(C), irradiation and PX478 group(D), irradiation combined AG490 and PX478 group(E). The size of xenograft tumor was measured and calculated. The expression of Ki67 and HIF-1α was detected by immunohistochemical method. Western blot was used to detect the expression of PARP1.RESULTS The size of xenograft tumor in group E was smaller compared with that in group C and group D. There were significantly difference between them respectively (t=12.367,11.598,P=0.000). The expression of HIF-1α in group E was lower than that in group C and group D respectively, and there were significantly difference respectively(t=5.422, 3.000,P<0.05). Ki67 index in group E was lower compared with that in group C and group D respectively and there were significantly difference respectively (t=4.479, 4.352,P<0.05). The level of cleaved PARP-1 in group E was higher than that in group C and group D respectively and there were significantly difference respectively (t=5.507, 7.102,P<0.05). CONCLUSION Combined inhibition of STAT3 and HIF-1α can increase the radiosensitivity of human laryngeal squamous carcinoma in the xenograft mice.

Bone turnover is regulated by local concentrations of cytokines such as osteoprotegerin (OPG) and receptor activator of nuclear factor kappaB ligand (RANKL). To explore the in vivo biological function of Opg and the mechanism of osteoporosis due to deficiency of Opg, Opg knockout mice have been generated through homologous recombination. Opg-/- mice exhibit a sharply decrease in bone density and strength as expected. The number of osteoclasts in Opg-/- mice significantly increases. Morphologically, osteoclasts appear more cuboidal in shape in Opg-/- mice than those of wt mice, suggesting that active osteoclastogenesis occurs in the absence of Opg. In consistent with this finding, an increase of osteoblast activity was also observed with accelerated mineral accumulation rate by histomorphometric measurement and elevated serum alkaline phosphatase activity (ALP) in Opg-/- mice. Interestingly, more than 50% of 2-month-old Opg-/- mice manifest medial calcification of aorta with comparable serum concentrations of calcium and phosphorus to wt mice. In conclusion, Opg-/- mice have a high-bone-rurnover type osteoporosis. The aortic calcification in Opg-/- mice is not due to abnormality of calcium and phosphorus metabolism. The mechanism underlying aortic calcification in Opg-/- mice needs to be further investigated.

Objective To observe the effects ofXiangsha Liujunzi Decoction (XSLJZ) on levels and mRNA of IL-6, IL-10 and protein expression of HSP70 of gastric mucosa in chronic atrophic gastritis (CAG) rats with spleen-stomach deficiency; To discuss its mechanism.Methods Rats were divided into 2 groups through random number table: normal group and model group. The model of CAG rat with spleen-stomach deficiency type was induced by synthetic methods. After successful modeling, rats were divided into model group, positive control group, XSLJZ high-, medium-, low-dose group. Rats in normal and model group received distilled water 10 mL/(kg?d) for gavage; XSLJZ high-, medium-, low-dose group received XSLJZ 24, 12, 6 g/(kg?d), respectively; positive control group received mycin 0.30 g/(kg?d) for gavage for 120 consecutive days. Generally living conditions, levels and mRNA of IL-6, IL-10 and protein expression of HSP70 in gastric mucosa tissue were detected by protein immunoblotting.Results Compared with the normal group, generally living conditions of rats in the model group were poor; mRNA and the content of IL-6 increased significantly, and mRNA and the content of IL-10 decreased significantly (P<0.05,P<0.01); mRNA and protein expression of HSP70 in gastric tissues was much lower than that of normal group (P<0.05,P<0.01). Compared with model group, generally living conditions of rats in the XSLJZ high-dose groups were improved significantly; mRNA and the content of IL-6 decreased significantly, and mRNA and the content of IL-10 increased significantly (P<0.05); XSLJZ high-, medium-dose groups mRNA and protein expression of HSP70 in gastric tissues increased significantly(P<0.05).Conclusion XSLJZ has protective effects on gastric mucosa of CAG rats with spleen-stomach deficiency.

Objective To study effects ofXiangsha Liujunzi Decoction (XSLJZ) on gastric emptying function, pepsin activity and expression of HIF-1α gene and protein of gastric mucosa in chronic atrophic gastritis (CAG) of rats with deficiency of spleen and stomach.Methods Rats were randomly divided into normal group and model group. The models of CAG rats with deficiency of spleen and stomach type were induced by synthetic methods. After the modeling, models rats were divided into model group, positive control group and XSLJZ high-, medium-, and low-dose groups. Normal group and model group were given 10 mL/kg distilled water every day for gavage; XSLJZ high-, medium-, and low-dose groups were given 24, 12, and 6 g/kg XSLJZ Decoction for gavage; positive control group was given 0.30 g/kg Vatacoenayme for gavage, for successive 120 d. Gastric emptying function and pepsin activity were detected, and HIF-1α gene and protein expression in gastric mucosa were detected by RT-qPCR and IHC. Results Compared with the normal group, the gastric emptying function and pepsin activity in the model group were much lower (P<0.01); expressions of HIF-1α gene and protein in gastric tissues was much higher (P<0.01). Compared with model group, XSLJZ could increase the gastric emptying function and pepsin activity significantly (P<0.05), and decrease expressions of HIF-1α gene and protein (P<0.05).Conclusion XSLJZ has functions of improving the gastric emptying function, promoting pepsin activity, and reducing expressions of HIF-1α gene and protein.

Objective: To understand the iodine nutritional status of residents in the newly identified high iodine and non-high iodine areas in Puyang, and to provide evidence for relevant departments to adjust the iodine supplementation policy. Methods: According to the survey results of drinking water iodine content in 2017, in 2018 stratified and systematic sampling methods were used to extract 25 villages in the newly identified high iodine areas and non-high iodine areas in Puyang City. Each village surveyed 40 children and 20 pregnant women, the urinary iodine content of the subjects and the salt iodine content at their home were measured, and the thyroid volume of children in 10 villages in high iodine and non-high iodine areas was measured. Results: In high iodine areas and non-high iodine areas, the household salt samples collected were 1 357 and 1 544, respectively, the iodized salt coverage rate was 87.91% (1 193/1 357) and 85.62% (1 322/1 544), respectively, and the difference was not statistically significant (χ² = 3.291, P > 0.05). A total of 1 028 children and 329 pregnant women in high iodine areas were investigated, their median urinary iodine content was 337.0 and 289.0 μg/L, respectively; a total of 1 041 children and 503 pregnant women in non-high iodine areas were investigated, their median urinary iodine content were 310.0 and 265.0 μg/L, the differences of the urinary iodine content between high iodine and non-high iodine areas were statistically significant (Z = -3.825, -2.795, P < 0.05). Thyroid volume was measured in 407 children in high iodine areas and 423 children in non-high iodine areas. The thyroid enlargement rate was 8.85% (36/407) and 3.78% (16/423), respectively, and the difference was statistically significant (χ² = 9.053, P < 0.05). Conclusions The iodine nutrition levels of children and pregnant women in high iodine areas are higher than those in non-high iodine areas. Iodized salt should be stopped supplying in high iodine areas as soon as possible. In non-high iodine areas, the feasibility of reducing the iodine concentration of iodized salt should be investigated to ensure the iodine nutrition level of local residents.