Objective To study the effects of perinatal exposure of diphenylchloroarsine chloride(DA)on the livability and reproductive function of F1 generation rats.Methods The pregnant rats were treated with DA by gavage at 0(solvent control),0.63(low dose),0.94(medium dose),1.89(high dose)mg/(kg?day)from day 6 of pregnancy to day 15 of lactation.The general condition,the change of body weight and abnormity of main organs of the F0 pregnant rats and F1 young rats were observed,and the livability and abnormity of reproductive function of F1 young rats were examined.Results The results indicated that compared with the negative control,the increasing amounts of the body weights of the F0 maternal rats and the livability of F1 young rats aged 4 day significantly decreased at 0.94 mg/kg and 1.89 mg/kg,the livability in lactation period obviously reduced at 1.89 mg/kg,and the rate of absorbed fetus in F1 pregnant rats increased at 1.89 and 0.94 mg/kg.Conclusion DA exposure may have an adverse effect on livability and reproductive function of the F1 offspring when the dosage is no fewer than 0.94 mg/kg.

A new embedded telemetry system for potentiometric sensors was developed. The system consisted of a transmitter unit, a receiver unit and a personal computer ( PC) . The transmitter unit included a current amplifier, a∑-Δanalog-to-digital converter ( ADC) , a microcontroller unit ( MCU) and a radio module. The receiver unit was composed of a radio module, a microcontroller unit and a serial-to-USB converter module. The receiver unit was connected to an upper computer via a universal serial bus ( USB ) . The embedded software written in C language controlled the signal acquisition and transmission. The computer software written in LabVIEW language was used for data storage and display. The range of the acquisition voltages was from-1. 17 V to +1. 17 V. In order to verify the accuracy and reliability of this system, a control experiment had been done with this system and a digital multimeter. Moreover, a response test of acidity changes had been done with the self-made H+ selective electrode. The results showed that the accuracy of this system could be up to 0. 1 mV and it had strong anti-noise ability. The square of the linear correlation coefficient of the response of the changes in pH values was 0 . 998 . The curve of the results measured by this system was consistent with that measured by the commercial electrochemical analyzer. The system was built with standard hardware components and the size of the transmitter unit was only 29 mm×14 mm×11 mm. It can be easily used for remote and real-time detection for the potentiometric sensor.

A new magnetic affinity immunoassay (MAIA) strategy based on enzyme-labeled phage displayed antibody was developed. The assay consisted of a sandwich format in which immobilized polyclonal antibody (pcAb) on magnetic microparticle was used for capture probe, and enzyme-labeled phage displayed antibody for specific detection probe to increase enzyme amount and enhance detection signal. By the proposed method,β-bungarotoxin (β-BGT) was successfully detected. A linear relationship between absorbance value and the concentration of β-BGT in the range of 0. 016-62. 5 μg / L was obtained. The linear regression equation was Y=0. 641X+1. 355 (R =0. 9925, n = 13, p<0. 0001) with a detection limit of 0. 016 μg / L. In comparison with the traditional ELISA, this method gave a 10-fold better sensitivity in β-BGT detection. This strategy also gave a 4-fold better sensitivity comparing with the MAIA based on enzyme labeled monoclonal antibody (mcAb). Due to low detection limit, acceptable reproducibility and high specificity, this method holds great promise in toxin trace detection.

A new micro embedded telemetry system was developed for the amperometric sensing detection. Its output voltage range was ±0. 5 V and resolution was<1 mV. The current acquisition range was ±1μA and the minimum resolution was 0. 2 nA. This telemetry system was designed based on microprocessor ADuCM360, including a potentiostat, a current detection module and a radio module. And the size was only 24 mm × 13 mm × 11 mm. The computer software written in LabVIEW language was used for data storage and display. In order to verify the accuracy and reliability of this system, an electrical performance test was performed. The current response for ascorbic acid with different concentration was recorded by using the telemetry system. The potential of working electrode was set as 30 mV, and the current response of ascorbic acid electrode had a good linear relationship with its concentration within the concentration range of 50-300 μmol/L. Linear equation was I(nA) = 2. 98CAA(μmol/L)-137. 39, and linear correlation coefficient R2=0. 984. Moreover, the applicability of the instrument in the study for living animals was explored by using the cerebral ischemia as model.

A new method for O-ethyl S-[2-( diisopropylamino) ethyl] methylphosphonothiolate ( VX) , sarin detection and its kinetic analysis based on piezoresistive microcantilever aptasensor was developed, where VX, sarin aptamers were immobilized on the microcantilever surface by biotin-avidin binding system. A linear relationship between the response voltage and the concentration of VX in the range of 2-60μg/L was obtained. The linear regression equation was △Ue=0. 886C-1. 039 (n=5, R=0. 984, p<0. 001) and the detection limit was 2μg/L ( S/N≥3 ) . A linear relationship between the response voltage and the concentration of sarin in the range of 10-60 μg/L was obtained, the linear regression equation was △Ue=0. 716C-2. 304 ( n=5, R=0 . 996 , p<0 . 001 ) and the detection limit was 10 μg/L ( S/N≥3 ) . The sensor showed no response for O-butyl methylphosphonochloridate, a structural analog of VX and sarin, which indicated high specificity and good anti-interference ability. On this basis, a reaction kinetic model based on receptor-ligand binding and the relationship with output voltage change was established. Response voltage (△Ue ) and response time( t0 ) were obtained from the fitting equation on different concentrations of VX, sarin fitted well with the measured values.