Objectives: To investigate the status of chlamydia pneumonia infection in blood donors from Nanjing Command of PLA. Methods: Use the technique of nested polymerase chain reaction to detect the DNA of chlamydia pneumonia in peripheral blood mononuclear cells. Results: 32 of 100 blood donors were positive (32%). Conclusions: Our study reveals that the infection rate of chlamydia pneumonia in blood donors from Nanjing Command of PLA is considerable high and the clinical values need further research.

Objective: To investigate the levels of resisitin and C reactive protein, and their relationship with diabetic in patients. Methods: The levels of resistin and C reactive protein were determined in 62 diabetic patients and 42 control subjects. Results: The levels of C reactive protein and resistin were (5.62?0.80))mg/L,(37.43?11.52)?g/L,respectively, compared with control subjects(C reactive protein: (3.62?1.1) mg/L, Resisitin: (18.24?5.84) ng/ml),P

To investigate weather serum soluble intercellular adhesion molecule-1 (sICAM-1) and hyaluronic acid(HA) levels can be used in assessing the disease activity of Graves' ophthalmopathy (GO).Serum levels of sICAM-1 and HA were determined in 48 cases with GO and 30 cases with Graves' disease without GO (CON).Compared with the CON group,the levels of sICAM-1 and HA in GO cases were raised significantly( P＜0.05 ).sICAM-1 and HA levels in the subgroup of patients with active GO were markedly elevated when compared to those with stable GO(P＜0.05 ).Pearson regression analysis showed that elevated levels of slCAM-1 and HA were positively correlated with the Clinical Activity Score ( CAS ) of GO ( r =0.53,P ＜ 0.01 ; r =0.46,P ＜ 0.01 ) ; A significant correlation between sICAM-1 and HA( r=0.31,P＜0.05 ) was noted.The raised serum levels of sICAM-1and HA may serve as two activity markers of GO.

Objective To explore the clinical effect of root cause analysis (RCA) in prevention of adverse events in hematodialysis center.Methods A total of 120 patients treated in our hospital carried out root cause analysis (RCA) management in hematology center from January 2016.The incidence of adverse events,the rate of reporting and nursing satisfaction were compared before and after RCA.Results After the implementation of RCA,the incidence of adverse events was significantly lower,and rate of reporting was improved than implementation before (P ＜ 0.05);After implementation of RCA,the incidence rates of nurse-patient disputes in hemodialysis center were significantly lower than that implementation before (P ＜ 0.05);After RCA,the patient's satisfaction with hemodialysis was significantly improved compared with treatment before (P ＜ 0.05).Conclusion RCA can effectively change traditional management concept,strengthen the safety of hemodialysis care,and improve working efficacy of nursing staff and nursing quality,reduce adverse events of hemodialysis and nurse-patient disputes,and enhance patients'satisfaction.

Objective To explore the clinical effect of root cause analysis (RCA) in prevention of adverse events in hematodialysis center.Methods A total of 120 patients treated in our hospital carried out root cause analysis (RCA) management in hematology center from January 2016.The incidence of adverse events,the rate of reporting and nursing satisfaction were compared before and after RCA.Results After the implementation of RCA,the incidence of adverse events was significantly lower,and rate of reporting was improved than implementation before (P ＜ 0.05);After implementation of RCA,the incidence rates of nurse-patient disputes in hemodialysis center were significantly lower than that implementation before (P ＜ 0.05);After RCA,the patient's satisfaction with hemodialysis was significantly improved compared with treatment before (P ＜ 0.05).Conclusion RCA can effectively change traditional management concept,strengthen the safety of hemodialysis care,and improve working efficacy of nursing staff and nursing quality,reduce adverse events of hemodialysis and nurse-patient disputes,and enhance patients'satisfaction.

Objective Using Meta-analysis method to evaluate the effect of multimedia video education and traditional education for hip replacement patients.Methods Databases including Elsevier, PubMed,EMbase,Medline,SCI,CNKI,CBM and WanFang Data were searched to collect the randomized controlled trails (RCTs) and non-RCTs regarding multimedia video education versus traditional education for hip replacement surgery patients. The retrieval time was from early available time to July 1st 2016.The data were collected and evaluated by two orthopedic nurses independently. The Meta-analysis was conducted by using RevMan5.2 software.Results A total of six RCTs and four non-RCTs involving 1197 patients were included. The results of Meta analysis showed that,the multimedia video education can improve the harris scores of one month [MD=6.95,95%CI(5.77,8.13),P<0.05],three months [MD=9.71,95%CI(7.53,11.89),P<0.05] and six months [MD=8.32,95%CI(4.46,12.17),P<0.05] after surgery,reduce the incidence of complications [OR=0.25,95%CI(0.15,0.42),P<0.05],decrease the hospital stays of patients [MD=-0.75,95%CI (-1.42,-0.08),P<0.05] and increase patients' awareness rate of rehabilitation knowledge [OR=2.55,95%CI (1.36,4.79),P<0.05].Conclusions Multimedia video education is superior to traditional education for hip replacement surgery patients,and with shorter hospital stays,lower complication rate postsurgery,multimedia video education should be promoted in clinic.

Objective: To report our experience in the prevention and control of COVID-19 in the tramatological and microsurgical wards. Methods: A retrospective study was conducted of the COVID-19 infections in the 51 medical staff and patients from 31 December, 2019 to 14 February, 2020 at Department of Traumatology and Microsurgery, Zhongnan Hospital. The prevention and control measures were upgraded after 20 January, 2020 to address the serious epidemic situation, including preventive disinfection, terminal disinfection and personnel disinfection in wards, management of emergency patients, inpatients and patients suspected of COVID-19 infection, and training, management and psychological intervention of medical staff. The outcomes resulting from different prevention and control measures before and after 20 January, 2020 were compared. Results: From 31 December, 2019 to 20 January, 2020, there were altogether 3 cases of definite COVID-19 infection and 2 ones of suspected COVID-19 infection at the department. One doctor, one technician and one nurse were diagnosed as definite COVID-19 infection while one nurse and one patient as suspected COVID-19 infection. The 4 medical staff members infected were cured and discharged before 14 February, 2020 but unfortunately the one patient infected died. After the prevention and control measures for COVID-19 infection had been upgraded since 20 January, 2020, 12 out of the 29 emergency patients at our wards had fever (body temperature ≥37.3℃) but none COVID-19 infection. All the 47 medical staff on duty at the department got trained and none of them was infected by COVID-19 or suffered from mental disorder. Conclusion In the epidemic of COVID-19, as our prevention and control measures for COVID-19 infection were adjusted and upgraded in response to the changing epidemic situation, they eliminated nosocomial infection scientifically and effectively and ensured life safety of the medical staff and patients at the department.

Objective:To investigate the radiation protection effect of furosemide intervention on 18F-2-deoxy-D-glucose ( 18F-FDG) positron emission tomography/computed tomography (PET/CT) imaging. Methods:A total of 146 patients were randomly divided into two groups, with test group of 74 patients and control group of 72. The test group was administrated orally with furosemide of 40 mg for each one before injection, while the normal control group did not undergo special treatment. 60 and 120 min after 18F-FDG injection, the horizontal measurement of ambient dose equivalent rates was carried out at 0.5 m from the front of both chest and abdomen respectively. Results:For the test group, the ambient dose equivalent rates were measured to be (30.80±8.61) and (41.38±11.06) μSv/h 60 min after injection of 18F-FDG whereas (18.26±4.85) and (24.66±6.50) μSv/h 120 min after injection, respectively, both lower than in the control group and with statistically significant difference between the both ( t =15.36, 13.13, 18.73, 17.29, P<0.05) . No significant difference was found between mediastinal SUV max and liver SUV max in the experimental group and control group ( P>0.05) . Multivariate ANOVA showed that body surface area was a major factor influencing ambient dose equivalent rate regardless of furosemide injection ( t=-13.52, 2.96, P<0.05) , and no obvious effects of age and sex on ambient dose equivalence rate were found. Conclusions:Furosemide intervention can promote urination, effectively reduce the internal radiation exposure of the examinated patietns in the premise of not affecting the image quality, and therefore provide a better radiation protection effect.

OBJECTIVEThe present study aims to study the influences experimental navigation and intensive exercise on immune-neuroendocrine network of the male rats and the effects of PNS to this influence.

METHODThirty 6-week Sprague-Dawley male rats (SD rats) were randomly located into three groups: Quiescent control (QC) group, training control (TC) group and Panax notoginseng saponins (PNS) group. Rats from QC group were not given any stimuli, and samples were taken after 7-day intragastric administration of saline. Rats from TC group underwent 10-day run training of increasing load on treadmill and received 7-day intragastric administration of saline. PNS group were subjected to the same procedure of run training as group TC, and received intragastric administration of PNS at the dose of 0.2 g x kg(-1). Blood samples were immediately obtained at the end of the tests to determine the serum levels of corticosterone (Cort), adrenocorticotropic hormone (ACTH), beta-endorphin (beta-EP), Neuropeptide Y (NPY) and interleukin-1beta (IL-1beta), interleukin-6 (IL-6) by RIA. Expressions of Pituitary ACTH, NPY were observed use the immunohistochemistry method and correlation analyses conducted. And the ultrastructural changes of the pituitaries and the adrenal cortex cells were examined by electron microscope meanwhile.

RESULTThe serum beta-EP and Cort levels in TC group were significantly increased compared to the QC group; whereas Expression levels of pituitary ACTH, NPY were markedly higher after experimental navigation and intensive exercise. In Group PNS, the plasma Cort, ACTH and NPY levels decreased significantly compared to Group TC. Meanwhile we found expression levels of pituitary ACTH, NPY also lower than group TC. There was no significant difference about the ultrastructure of anterior pituitary and adrenal cortex of QC and PNS group. However, a obvious change of ultrastructure occurred to TC group rats.

CONCLUSIONThese results suggest that immune-neuroendocrine network function of rats were confused by negative psychological stresses and intensive exercise. PNS therapy may exert regulation effects to the network.

Animals ; Drugs, Chinese Herbal ; administration & dosage ; Exercise ; Humans ; Immune System ; drug effects ; metabolism ; Male ; Models, Animal ; Neurosecretory Systems ; drug effects ; metabolism ; Panax notoginseng ; chemistry ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Saponins ; administration & dosage

OBJECTIVETo study Chlamydia pneumoniae (C. pneumoniae) infection in 110 patients with respiratory tract infection admitted to our hospital from January to December 1995 in Nanjing.

METHODSSputum and throat swab specimens were taken and C. pneumoniae DNA was detected by using polymerase chain reaction (PCR) with the HM-1-HR-1 primer pair. At the same time, serum samples were taken and immunoglobulin G and M (IgG and IgM) fractions of antibodies to C. pneumoniae were studied by microimmunofluorescence test.

RESULTSPrevalence of specific IgG was 70% in patients with respiratory tract infection. Seventeen patients (15.5%) were serologically diagnosed as having recent C. pneumoniae infections and 12 patients (10.9%) had positive PCR in sputum and/or swab specimens. The total positive rate was 22.7% (25/110) detected by PCR combined with serological tests. Acute infection of C. pneumoniae was common in patients with asthma (57.1%), pneumonia (35.0%), COPD (25.9%) and bronchitis (25.0%). Clinical features between C. pneumoniae infection and non-C. pneumonia infection showed no significant differences.

CONCLUSIONSChlamydia pneumoniae is an important pathogen that causes infection of the human respiratory tract and attention should be drawn to this special illness.

Acute Disease ; Adolescent ; Adult ; Age Factors ; Aged ; Aged, 80 and over ; Antibodies, Bacterial ; blood ; Chlamydophila pneumoniae ; genetics ; immunology ; DNA, Bacterial ; analysis ; Female ; Humans ; Immunoglobulin G ; blood ; Male ; Middle Aged ; Pneumonia, Bacterial ; blood ; microbiology ; Polymerase Chain Reaction