1.Brief accout of stem cell application in treating myocardial infarction
Chongyang WU ; Lanjun SUN ; Yingqiang ZHAO
Basic & Clinical Medicine 2006;0(12):-
Myocardial infarction is one of the main factors causing heart failure and is associated with a high rate of morbidity and mortality in the developing countries.Due to infracted cardiac muscles,heart function will aggravate and myocardium will remodel leading to exacerbation of the disease.Current treatment modalities may not be adequate to prevent myocardial remodeling.It is necessary to explore a new approach to restore the damaged myocardium.Stem cell-based therapy is developing rapidly and has been used in the field of cardiovascular disease.There are many problems still to be solved.
2.Influence of precision medicine-based health education on cognitive level of disease knowledge, anxiety and depression in patients with gestational diabetes
Zhaona SUN ; Yunyun LI ; Lanjun WU ; Qingxian ZHU ; Xiaoyan WU
Modern Clinical Nursing 2017;16(1):38-42
Objective To study the influence of precision medicine-based health education on cognitive level of disease knowledge,anxiety and depression in patients with gestational diabetes.Methods Toally 84 cases of gestational diabetes patients in our hospital from September 2014 to December 2015 were divided into a study group and a control group according to the random number table,42 cases in each group.While the routine nursing intervention was done in the control group,the precision medicinebased health education program was carried out in the study group.The two groups were assessed using the cognitive questionnaire designed by our hospital.The self rating anxiety scale (SAS) and self rating depression scale (SDS) prepared by Zung were used to assess the levels of anxiety and depression in the two groups before the intervention and two weeks after the intervention.Results The cognitive level of disease knowledge in the study group after the intervention was statistically significantly higher than that of the control group (P<0.05).Before the intervention,there were statistically insignificant differences in anxiety and depression between the two groups (P>0.05),but after the intervention,the levels of anxiety and depression in the study group were significantly lower than those of the control group (P<0.05).Conclusion The precision medicine-based health education is helpful for the patients with gestational diabetes to improve their diabetes-related knowledge and meanwhile it can relieve their anxiety and depression.
3.Characterization of acute renal allograft rejection by human serum proteomic analysis.
Ying, GAO ; Ke, WU ; Yi, XU ; Hongmin, ZHOU ; Wentao, HE ; Weina, ZHANG ; Lanjun, CAI ; Xingguang, LIN ; Zemin, FANG ; Zhenlong, LUO ; Hui, GUO ; Zhonghua, CHEN
Journal of Huazhong University of Science and Technology (Medical Sciences) 2009;29(5):585-91
To identify acute renal allograft rejection biomarkers in human serum, two-dimensional differential in-gel electrophoresis (2-D DIGE) and reversed phase high-performance liquid chromatography (RP-HPLC) followed by electrospray ionization mass spectrometry (ESI-MS) were used. Serum samples from renal allograft patients and normal volunteers were divided into three groups: acute rejection (AR), stable renal function (SRF) and normal volunteer (N). Serum samples were firstly processed using Multiple Affinity Removal Column to selectively remove the highest abundance proteins. Differentially expressed proteins were analyzed using 2-D DIGE. These differential protein spots were excised, digested by trypsin, and identified by RP-HPLC-ESI/MS. Twenty-two differentially expressed proteins were identified in serum from AR group. These proteins included complement C9 precursor, apolipoprotein A-IV precursor, vitamin D-binding protein precursor, beta-2-glycoprotein 1 precursor, etc. Vitamin D-binding protein, one of these proteins, was confirmed by ELISA in the independent set of serum samples. In conclusion, the differentially expressed proteins as serum biomarker candidates may provide the basis of acute rejection noninvasive diagnosis. Confirmed vitamin D-binding protein may be one of serum biomarkers of acute rejection. Furthermore, it may provide great insights into understanding the mechanisms and potential treatment strategy of acute rejection.
4.Roles of aquaporin 4 in intracerebral hemorrhage
Xinyu WANG ; Lanjun LI ; Yuying SUN ; Danhua DING ; Qianqian LI ; Jun WU
International Journal of Cerebrovascular Diseases 2017;25(9):839-843
Aquaporin 4 (AQP4) is the most abundant aquaporin type in the brain.It is mainly expressed in the perivascular end feet of astrocytes.A large number of studies have shown that AQP4 is involved in the formation and elimination of brain edema in intracerebral hemorrhage,and plays important roles in the maintenance of the integrity of blood-brain barrier,secondary neuroinflammation,and apoptosis after intracerebral hemorrhage.More and more studies focus on the roles and mechanisms of AQP4 in intracerebral hemorrhage,however,the results are not completely consistent.This article reviews the roles and mechanisms of AQP4 in intracerebral hemorrhage
5.Recent advance in microglial cell drug modulation in ischemic stroke
Lanjun LI ; Yinyu WANG ; Yuying SUN ; Yuming XU ; Jun WU
Chinese Journal of Neuromedicine 2017;16(6):638-642
The etiology underlying ischemic stroke is still elusive. Previous studies have indicated that inflammation might play a key role in the pathogenesis, which provided a novel insight into the therapeutic strategy of ischemic stroke. In this review, we summarize some drugs which regulate the activation and polarization of microglia and further alleviate neurological symptoms.
6.Recent advance in gene polymorphisms of inflammatory cytokines in Alzheimer's disease
Qiangian LI ; Lanjun LI ; Danhua DING ; Shuang ZHANG ; Jun WU
Chinese Journal of Neuromedicine 2018;17(6):640-644
The central nervous system inflammatory response plays an important role in the pathogenesis of AD.Pro-inflammatory cytokines can induce inflammatory reactions in the body,enhance the neurotoxic effects caused by Aβ,promote the pathogenesis of AD and anti-inflammatory factors can down-regulate the inflammatory response,play a protective role and promote the reconstruction of damaged tissue.However,due to the existence of genetic polymorphisms,the influence of inflammatory factors on AD is complicated,especially in different population groups.Studing and clarifying the relationship between gene polymorphisms of inflammatory factors and and the pathogenesis of AD will promote the study of the mechanism of AD and provide a new method for the treatment of AD.Therefore,this article mainly reviews this.
7.Mechanism of microglia cell activation and differentiation induced by lipopolysaccharide
Jun WU ; Danhua DING ; Qianqian LI ; Xinyu WANG ; Yuying SUN ; Lanjun LI
Chinese Journal of Neuromedicine 2018;17(12):1195-1202
Objective To observe the effect and molecular mechanism of lipopolysaccharide (LPS) on activation and differentiation of microglia (MG) cells. Methods Routinely in vitro cultured BV2 microglia cells were divided into control group and LPS group: BV2 microglia cells in the LPS group were treated with 200 ng/mL LPS; cells in the control group were added the same amount of medium. Six h after treatment, real-time quantitative (qRT)-PCR and enzyme-linked immunosorbent assay (ELISA) were used to detect the inflammatory factors, interleukin (IL)-1β and tumor necrosis factor (TNF)-α mRNA and protein expressions in supernatant of cell culture medium. The iNOS, CD32, Arg1 and CD206 mRNA and protein expressions were detected by qRT-PCR and immunofluorescence, respectively. The mRNA and protein expressions of Notch1, Hes1 and Hes5 were detected by qRT-PCR and Western blotting. Results After LPS stimulation, BV2 microglia cells were activated and the morphological changes were observed. The IL-1β and TNF-α protein and mRNA expressions in the LPS group were significantly increased as compared with those in the control group (P<0.05). The iNOS and CD32 protein and mRNA expressions in the LPS group were significantly increased as compared with those in the control group (P<0.05). The Arg1 and CD206 mRNA and protein expressions showed no significant differences between the two groups (P>0.05). The Notch1 and Hes1 mRNA and protein expressions in the LPS group were significantly increased as compared with those in the control group (P<0.05), while no significant differences on Hes5 mRNA and protein expressions were noted between the two groups (P>0.05). Conclusion LPS activates MG cells, which may regulate the differentiation of MG cells into M1 through Notch signaling pathway and promote inflammatory response; therefore, Notch signaling pathway may be a target for regulating MG cells differentiation and reducing inflammatory damage.
8.Effects of empowerment management model based on Myers-Briggs Type Indicator Scale on the quality of orthopedic nursing management
Yan LI ; Lanjun ZHANG ; Hangyan SUN ; Yu SUN ; Ling QIU ; Wanzhen WU
Chinese Journal of Modern Nursing 2021;27(2):241-244
Objective:To explore the effect of implementing group empowerment management model based on Myers-Briggs Type Indicator (MBTI) Scale on the quality of orthopedic nursing management.Methods:From January 2018 to December 2019, MBTI Scale was used to test the personality types of 46 nurses in Department of Orthopedics in Zhejiang Hospital. Test results were analyzed and nurses were grouped and given empowerment management measures. Score of orthopedic nurses on the leaders, score of psychological empowerment of nurses and score of nursing quality were compared before and after the improvement of management.Results:MBTI Scale was used to classify personality types of 46 orthopedic nurses, among which ISFJ, ISTJ, ENFP and ESFJ were the most common personality types. After the implementation of empowerment management, score of nurses on the leaders, score of psychological empowerment of nurses and score of nursing quality were all improved than before, and the differences were statistically significant ( P<0.05) . Conclusions:Based on MBTI Scale, the group empowerment management of orthopedic nurses can give full play to the core advantages of nurses, improve self-management ability of nurses and nursing quality.
9. Thoughts and principles of diagnosis and treatment of chronic refractory wounds in China
Wei DONG ; Yurui XIAO ; Minjie WU ; Duyin JIANG ; Lanjun NIE ; Yingkai LIU ; Jiajun TANG ; Ming TIAN ; Chunlan WANG ; Lifang HUANG ; Jiaoyun DONG ; Xiaozan CAO ; Fei SONG ; Xiaoyun JI ; Xian MA ; Yutian KANG ; Shuwen JIN ; Chun QING ; Shuliang LU
Chinese Journal of Burns 2018;34(12):868-873
The correct thoughts and principles of diagnosis and treatment of chronic refractory wounds need to be formulated. Through the relevant domestic and international consensus and based on clinical experience, the
10.Characterization of Acute Renal Allograft Rejection by Human Serum Proteomic Analysis
GAO YING ; WU KE ; XU YI ; ZHOU HONGMIN ; HE WENTAO ; ZHANG WEINA ; CAI LANJUN ; LIN XINGGUANG ; FANG ZEMIN ; LUO ZHENLONG ; GUO HUI ; CHEN ZHONGHUA
Journal of Huazhong University of Science and Technology (Medical Sciences) 2009;29(5):585-591
To identify acute renal allograft rejection biomarkers in human serum, two-dimensional differential in-gel electrophoresis (2-D DIGE) and reversed phase high-performance liquid chromatog-raphy (RP-HPLC) followed by electrospray ionization mass spectrometry (ESI-MS) were used. Serum samples from renal allograft patients and normal volunteers were divided into three groups: acute rejec-tion (AR), stable renal function (SRF) and normal volunteer (N). Serum samples were firstly processed using Multiple Affinity Removal Column to selectively remove the highest abundance proteins. Differ-entially expressed proteins were analyzed using 2-D DIGE. These differential protein spots were ex-cised, digested by trypsin, and identified by RP-HPLC-ESI/MS. Twenty-two differentially expressed proteins were identified in serum from AR group. These proteins included complement C9 precursor,apolipoprotein A-Ⅳ precursor, vitamin D-binding protein precursor, beta-2-glycoprotein 1 precursor,etc. Vitamin D-binding protein, one of these proteins, was confirmed by ELISA in the independent set of serum samples. In conclusion, the differentially expressed proteins as serum biomarker candidates may provide the basis of acute rejection noninvasive diagnosis. Confirmed vitamin D-binding protein may be one of serum biomarkers of acute rejection. Furthermore, it may provide great insights into un-derstanding the mechanisms and potential treatment strategy of acute rejection.