1.Concentration and determination of strychnine alkaloid in biological fluids.
Jing ZHANG ; Lang-chong HE ; Qiang FU
Journal of Forensic Medicine 2005;21(1):36-38
OBJECTIVE:
To establish a new method for determination of strychnine alkaloid in biological fluids based on molecularly imprinted polymers.
METHODS:
A strychnine molecularly imprinted monolithic column was prepared by in-situ molecularly imprinted technique. The polymer was filled to a 1cm column, and a method was developed to concentrate and determine strychnine alkaloids in biological fluids.
RESULTS:
the limit of detection of the method was 4.9 ng, and the recoveries were more than 92%. The relative standard deviations were smaller than 6.59%. The linear correlation coefficients of standard curves were 0.999 1 and 0.9966 respectively. This method was applied to concentrate and determine strychnine in plasma and urine of poisoned rabbit.
CONCLUSION
The new method could concentrate and simultaneously determine strychnine alkaloids in biological fluids, and it was applied to forensic toxicological analysis.
Alkaloids/analysis*
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Animals
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Chromatography, High Pressure Liquid/methods*
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Humans
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Male
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Polymers/chemistry*
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Rabbits
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Sensitivity and Specificity
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Strychnine/urine*
2.Inhibitory effects of ligustilide and butylidenephthalide on bFGF-stimulated proliferation of rat smooth muscle cells.
Ming-jin LIANG ; Lang-chong HE
Acta Pharmaceutica Sinica 2006;41(2):161-165
AIMTo investigate the bio-affinities of ligustilide and butylidenephthalide to rat aortic smooth muscle cells and the inhibitory effects of them on bFGF-stimulated proliferation of rat vascular smooth muscle cell (VSMC).
METHODSVSMCs were cultured from rat aorta pectoralis and identified by an immunohistochemical method. The bio-affinities between solute (ligustilide or butylidenephthalide) and cell membrane were measured by rat aortic cell membrane chromatography (CMC). The inhibitory effects of ligustilide and butylidenephthalide on bFGF-stimulated VSMC proliferation were evaluated by MIT colorimetric method.
RESULTSBoth ligustilide and butylidenephthalide had selective affinities to rat aortic smooth muscle cell as the same as verapamil, one of the calcium ion antagonists. They could potently inhibit the bFGF-stimulated VSMC proliferation at the concentrations of 5.5 and 11.1 micromol x L(-1), separately (P < 0.05), but had no effects on the normal VSMC growth.
CONCLUSIONBoth ligustilide and butylidenephthalide can inhibit the abnormal proliferation of VSMC induced by bFGF.
4-Butyrolactone ; analogs & derivatives ; isolation & purification ; pharmacology ; Animals ; Aorta, Thoracic ; cytology ; Cell Proliferation ; drug effects ; Cells, Cultured ; Female ; Fibroblast Growth Factor 2 ; antagonists & inhibitors ; Ligusticum ; chemistry ; Male ; Muscle, Smooth, Vascular ; cytology ; drug effects ; Myocytes, Smooth Muscle ; cytology ; drug effects ; Phthalic Anhydrides ; isolation & purification ; pharmacology ; Plants, Medicinal ; chemistry ; Rats ; Rats, Sprague-Dawley
3.Studies on differences of pharmacokinetic behavior and tissue distribution of nimodipine and its two enantiomers in rats using achiral and chiral liquid chromatography.
Acta Pharmaceutica Sinica 2003;38(8):603-608
AIMTo investigate the differences of pharmacokinetic behavior and tissue distribution of nimodipine and its two enantiomers in rats.
METHODSA high-performance liquid chromatographic method with an ODS column (150 mm x 4.6 mm ID) and a mobile phase of methanol-water (70:30) was used for racemic nimodipine assay. Another method with a Chiralcel OJ column (250 mm x 4.6 mm ID) and a mixture of n-haxane-ethanol (85:15) as mobile phase was used to determine its two enantiomers. Nimodipine was monitored at 236 nm wavelength.
RESULTSThe linearity, recoveries and the detection limits of the methods were found to be suitable for the determinations. The average results of within-day and between-day RSDs were 5.64% and 7.85% respectively, the mean recovery was 97.66% for the concentration ranges studied. The pharmacokinetic parameters Tmax, Cmax, AUC and CLs were: S-(-)-nimodipine (2.1 +/- 0.3) h, (197 +/- 5) microgram.L-1, (656 +/- 18) mL.min-1, (0.30 +/- 0.03) microgram.h.L-1, and R-(+)-nimodipine (1.7 +/- 0.5) h, (128 +/- 4) microgram.L-1, (381 +/- 4) mL.min-1, (0.53 +/- 0.03) microgram.h.L-1, respectively. The S-(-)-nimodipine concentration was 2.23 and 1.97 times as high as that of R-(+)-nimodipine in heart and in cerebrum respectively and there was almost only S-(-)-nimodipine in cerebellum. But R-(+)-nimodipine concentration was 1.57, 3.69 and 4.20 times as high as that of S-(-)-nimodipine in major excretion organs such as kidney, spleen and liver respectively.
CONCLUSIONThe experimental results obtained by using the achiral and chiral liquid chromatography showed that the differences between enantiomers were apparent for the pharmacokinetics in rat plasma, and very significant for the distributions in major target tissues: heart, cerebrum and cerebellum, and main elimination tissues: kidney, spleen and liver.
Animals ; Area Under Curve ; Calcium Channel Blockers ; blood ; chemistry ; pharmacokinetics ; Chromatography, High Pressure Liquid ; methods ; Female ; Male ; Nimodipine ; blood ; chemistry ; pharmacokinetics ; Rats ; Stereoisomerism ; Tissue Distribution
4.Applications of HPLC/MS in the analysis of traditional Chinese medicines
Miao LI ; Xiaofang HOU ; Jie ZHANG ; Si-Cen WANG ; Qiang FU ; Lang-Chong HE ;
Journal of Pharmaceutical Analysis 2011;01(2):81-91
In China, traditional Chinese medicines (TCMs) have been used in clinical applications for thousands of years. The successful hyphenation of high-performance liquid chromatography (HPLC) and mass spectrometry (MS) has been applied widely in TCMs and biological samples analysis. Undoubtedly, HPLC/MS technique has facilitated the understanding of the treatment mechanism of TCMs. We reviewed more than 350 published papers within the last 5 years on HPLC/MS in the analysis of TCMs. The present review focused on the applications of HPLC/MS in the component analysis, metabolites analysis, and pharmacokinetics of TCMs etc. 50% of the literature is related to the component analysis of TCMs, which show that this field is the most popular type of research. In the metabolites analysis, HPLC coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry has been demonstrated to be the powerful tool for the characterization of structural features and fragmentation behavior patterns. This paper presented a brief overview of the applications of HPLC/MS in the analysis of TCMs. HPLC/MS in the fingerprint analysis is reviewed elsewhere.
5.Inhibitory effect of taspine on mouse S180 sarcoma and its mechanism.
Yan-Min ZHANG ; Lang-Chong HE ; Hong-Ying WANG
China Journal of Chinese Materia Medica 2007;32(10):953-956
OBJECTIVETo study the inhibition effect of taspine on mouse S180 sarcoma and its mechanism.
METHODThe mouse S180 sarcoma model was established and used to observe the antitumor activity of taspine. The microvessel density and protein expressing of the VEGF, bFGF, Bcl-2 and Bax in the tumor were measured by immunohistochemistry.
RESULTTaspine showed antitumor activity on the mouse S180 sarcoma in a good dose-dependent manner. The inhibition rates on tumor of taspine at low, middle and high concentrations were 39.08% , 43.99% and 48.60%, respectively. The microvessel density and protein expressing of the VEGF, bFGF, Bcl-2 and Bax in the tumor were decreased compared with the negative control. The ratio of Bax to Bcl-2 was increased.
CONCLUSIONTaspine has antitumor effect on the S180 sarcoma, and the mechanism may be through the way of decreasing the expressing of the VEGF, bFGF, Bcl-2 and Bax and inducing the vascular endothelial cell apoptosis.
Alkaloids ; isolation & purification ; therapeutic use ; Animals ; Antineoplastic Agents, Phytogenic ; isolation & purification ; therapeutic use ; Berberidaceae ; chemistry ; Cell Line, Tumor ; Dose-Response Relationship, Drug ; Fibroblast Growth Factor 2 ; metabolism ; Male ; Mice ; Neovascularization, Pathologic ; metabolism ; prevention & control ; Phytotherapy ; Plant Roots ; chemistry ; Plants, Medicinal ; chemistry ; Proto-Oncogene Proteins c-bcl-2 ; metabolism ; Sarcoma 180 ; blood supply ; pathology ; prevention & control ; Vascular Endothelial Growth Factor A ; metabolism ; bcl-2-Associated X Protein ; metabolism
6.Protecting effect of brevifolin and 8,9-single-epoxy brevifolin of Phyllanthus simplex on rat liver injury.
Xiao-Feng NIU ; Lang-Chong HE ; Te FAN ; Yan LI
China Journal of Chinese Materia Medica 2006;31(18):1529-1532
OBJECTIVETo investigate the effect of protecting liver of brevifolin and 8,9-single-epoxy brevifolin of Phyllanthus simplex.
METHODRats were administered with CCl4 (ip) or alcohol (ig) to establish acute or chronic liver injured model, respectively. ALT, AST and TBIL in serum were measured using colorimetric analysis to evaluate liver function. MDA content or SOD activity in serum and liver tissue was measured by thiobarbituric acid chromatometry and xanthine oxidase methods, respectively. The hemorheological parameters were observed.
RESULTBrevifolin and 8,9-single-epoxy brevifolin reduced the increase of ALT induced by CCl4, but they did not influence the increase of AST. And it could inhibit the pathologic increase of serum TBIL induced by alcohol. They could ameliorate the MDA increase or SOD decrease in serum and liver tissue in rats with liver injury, and decrease abnormal changed hemorheological parameters.
CONCLUSIONBrevifolin and 8,9-single-epoxy brevifolin show protective effective against acute and chronic liver injuries, and the mechanism is relevant to antagonizing the lipid peroxidation of free radical and improving the blood circulation.
Animals ; Carbon Tetrachloride Poisoning ; Chemical and Drug Induced Liver Injury ; physiopathology ; Drugs, Chinese Herbal ; isolation & purification ; pharmacology ; Female ; Hemorheology ; drug effects ; Hepatitis, Alcoholic ; physiopathology ; Liver ; physiopathology ; Male ; Phyllanthus ; chemistry ; Plants, Medicinal ; chemistry ; Protective Agents ; isolation & purification ; pharmacology ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Taxoids ; isolation & purification ; pharmacology
7.Analysis of supercritical fluid extracts of Radix caulophylli with gas chromatography-mass spectrometry.
Si-Cen WANG ; Qin-Hua CHEN ; Yao-Yuan WEI ; Han-Wen LI ; Lang-Chong HE
Acta Pharmaceutica Sinica 2007;42(5):525-528
To analyze the constituents in supercritical fluid CO2 extraction (SFE-CO2) of Radix caulophylli, the Radix caulophylli was extracted with SFE-CO2, and analyzed by gas chromatography-mass spectrometry (GC-MS). The GC-MS analysis with a DB-5MS capillary column (30 mm x 0.32 mm ID, 0.25 microm film thickness) was used. The inlet temperature was maintained at 280 degrees C. The column oven was held at 80 degrees C for 2 min, then programmed from 80 to 280 degrees C at 5 degrees C x min(-1) and, finally, held for 4 min. Helium at a constant flow rate of 2.0 mL x min(-1) was used as the carrier gas. The mass spectrometry conditions were as follows: ionization energy, 70 eV; ion source temperature, 200 degrees C. The mass selective detector was operated in the TIC mode (m/z was from 40 - 500). For the first time 49 peaks were separated and identified, the compounds were quantitatively determined by normalization method, and the identified compounds represent 97.44% of total GC peak areas. Viz, n-hexadecanoic acid (31.4%), (E, E) -9, 12-octadecadienoic acid (26.54%), (Z)-7-tetradecenal (9.4%), hexadecenoic acid (3.23%), 10-undecenal (3.22%), octadecanoic acid (2.25%), and caulophylline (1.76%) etc. The results will provide important foundation for understanding the constituents and further exploitation of Radix caulophylli.
Carbon Dioxide
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Caulophyllum
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chemistry
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Chromatography, Supercritical Fluid
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Gas Chromatography-Mass Spectrometry
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Linoleic Acid
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analysis
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Palmitic Acid
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analysis
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Plant Roots
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chemistry
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Plants, Medicinal
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chemistry
8.A method for improving the accuracy and sensitivity of cell membrane chromatography.
Yu WANG ; Yu WANG ; Xiu-ling DENG ; Bing-xiang YUAN ; Lang-chong HE
Journal of Southern Medical University 2009;29(12):2362-2366
OBJECTIVETo improve the accuracy and sensitivity of cell membrane chromatography (CMC) and evaluate the feasibility of CMC in the study of subtype receptors.
METHODSPlasmids were used to transfer alpha(1B)-AR cDNA into human embryonic kidney 293 (HEK293) cell lines to obtain cell lines stably overexpressing the subtype receptors. HEK293 alpha(1B) cell membrane stationary phase (CMSP) was prepared by immobilizing the cell membrane on silica. The retention time of 9 alpha(1)-adrenoceptor ligands and capacity factors(kappa'(HEK293 alpha1B)) were calculated. The capacity factors of rat liver tissue and primary cultured rat hepatocytes were also calculated for a correlation analysis.
RESULTSThe calculated capacity factors (kappa') were positively correlated to the published pKi values. The affinity rank orders were identical. The longest retention of the 9 alpha(1)-adrenoceptor ligands occurred on CMSP prepared with HEK293 alpha(1B) cell lines, while CMSP obtained from rat liver tissue showed the shortest retention of the ligands.
CONCLUSIONCMC proves practical in the study of the subtype adrenoceptors. The accuracy and sensitivity of CMC can be improved using HEK293 alpha(1B) cell membrane.
Animals ; Cell Membrane ; metabolism ; Chromatography, Affinity ; methods ; DNA, Complementary ; metabolism ; Female ; HEK293 Cells ; Humans ; Kidney ; cytology ; embryology ; Ligands ; Male ; Rats ; Rats, Sprague-Dawley ; Receptors, Adrenergic, alpha-1 ; metabolism ; Sensitivity and Specificity
9.Preliminary study on the mechanism of anaphylactoid reaction induced by natural product germacrone
Jia-pan GAO ; De-lu CHE ; Tao ZHANG ; Lang-chong HE
Acta Pharmaceutica Sinica 2019;54(9):1667-1672
The Chinese medicine injections prepared by the natural products containing sesquiterpenoids caused various adverse reactions in clinical use, among which skin allergic reactions are the most common. However, whether the reason of allergic reaction was related to the three isoprene units contained in the sesquiterpenoids is not clear, so the evaluation of drug safety has important guiding significance. The sesquiterpenoids are small molecular substances, and they are not antigens or haptens. They may induce anaphylaxis reactions by acting mast cells directly. Current research confirmed that Mas-related G protein-coupled receptor-X2 (MRGPRX2) which is a 7-transmembrane G protein coupled receptor on mast cells was a key target mediated allergic reactions induced by many small molecular drugs. Unlike IgE-mediated allergic reactions, pseudo-allergic reaction is related to dosage and dosing rate, and occurs in the first exposure to the sensitizer. In this paper, a series of experiments