Objective:To study the correlation of the concentrations of tumor necrosis factor ?(TNF-?)between saliva and serum in patients with recurrent aphthous ulceration(RAU).Methods:Thirty patients with light RAU and thirty healthy controls were recruited.The levels of tumor necrosis factor ?(TNF-?)were measured in both saliva and serum of patients with RAU and control subjects by enzyme linked immunosorbent assay(ELISA)and TNF-? standard curve.Results:The concentrations of tumor necrosis factor ?(TNF-?)in saliva and serum were significantly higher(P

10%～20% of acute clinical sporatic hepatitis is hepatitis E (HE). HE has a severe diaease low mortality in general population of about 0.5～1.5%. It has been reported as severe disease and the mortality increased to 20% in pregnant women and old people. China is high-epidemic for hepatitis E virus (HEV). HEV is mainly transmitted through faecaloral route,data also showed that HEV could be transferred through blood donation. The epidemiology,diagnostics and vaccine development of HE is reviewed in this paper.

Diabetic retinopathy is one of the most common and serious chronic complications. Recently, great quantities of researches show that there are correlations between the oxidative stress and the diabetic retinopathy. Hyperglycemia causes the oxidative stress, and it damages the retina through injuring the endothelial cells and mitochondria, generating abnormal metabolism pathway and promoting the inflammatory response, then the serious consequences have been caused such as retinal ischemia or even detachment. In clinic the antioxidant treatment has achieved some results. So this paper focuses on the relationship of the oxidative stress and the incidence of the diabetic retinopathy.

Objective To establish a differently expressed genetic profile in placental tissues of pregnancy induced hypertension(PIH). Method The total RNAs were isolated from the tissues by Trizol and mRNA was purified by Midi Kit. The expression of 17 000 genes in placental tissues of 6 PIH and 5 normoten sive women were evaluated by cDNA array technique. Results Ninety-six differently expressed genes were identified in placental tissue of PIH compared with normal pregnancy cases. Seventy-eight genes were up-regulated and 18 were down-regulated. There were 6 unclassified genes, 8 unknown expressed sequence tag (EST) fragments and 3 cDNA fragments among these differently expressed genes. The differently expressed genes were involved in transcription and translation,cell differentiation,receptor,apoptosis,immune,metabolism and growth. Conclusion The cellular mechanism of PIH involves changes in genes expression in placental tissue. Microarray is a useful method to identify differently expressed genes in tissue.

Objective To detect the level of nitric oxide (NO) and amino acids in cerebrospinal fluid and serum of epilepsy children in different time. Methods The level of NO and amino acids in cerebrospinal fluid and serum of epilepsy children in different time were determined with nitrate reductase and high performance liquid chromatography (HPLC) with fluorimetric detection. Results The level of NO2 - increased 30 min, 60 min, 120 min after seizures (P<0.01), but dropped to normal 180 min after seizures (P>0.05). The level of glutamate (Glu) significantly increased in all epilepsy groups (P<0.05). The level of glycine (Gly) dropped gradually in all epilepsy groups (P<0.05).Conclusion NO may participate in the generation of seizures and the injury process to brain. The imbalance between excitatory and inhibitory amino acids may play an important role in the pathogenesis of epilepsy. The toxicity of excitatory amino acids may be the main injury to the brain.

Crohn Disease ; Humans ; Infant, Newborn ; Male

ObjectiveTo study of the impact on dose distribution by electron width of energy spectrum and angular distribution using Monte Carlo simulation.MethodsThe simulated electron energy spectrum and angular distributions was as the input parameters and percentage depth dose (PDD) and offaxis curves were simulated by a modified PENELOPE code package. Results PDD and off-axis dose distribution curves are almost the same and are not sensitive to energy spectrum width and angular distribution with the exception of energy spectrum width of 2.5 MeV with obviously different curves.ConclusionsIn the situation of clinical treatment,spectrum and angular distributions can be ignored when their width are not very large.It is helpful to save about 9％ time by using monoenergy beams in treatment planning system development.

Objective To explore the diagnostic value of alcohol fixation and liquid-based cytology method for malignant stricture of common bile duct and to study the interval time between sampling and fixation for procedure of alcohol fixation.Methods Data of 82 patients were retrospectively studied,who were suspected of having malignant stricture of common bile duct underwent brushing through endoscopic retrograde cholangiopancreatography (ERCP),and were confirmed by follow-up for 2 years or pathology after surgery from October 2008 to June 2013.These patients were randomly divided into alcohol fixation group (n =30),liquid-based cytology group (n =28) and conventional cytology group (n =24).The positive diagnostic rates of brush cytology in each group were compared.The alcohol fixation group were randomly divided into 2-minute group and 5-minute group according to the interval time between sampling and fixation.Positive diagnostic rates were also compared.Results The positive rates were 73.33％ (alcohol fixation group,22/30),75.00％ (liquid-based cytology group,21/28) and 20.83％ (conventional cytology group,5/24),respectively.The positive rate of conventional cytology group was obviously lower than alcohol fixation group (x2 =21.525,P=0.000) and liquid-based cytology group (x2 =22.208,P =0.000) with statistical significance.But the positive rate of alcohol fixation group was similar to the liquid-based cytology group (x2 =0.683,P =0.898).In alcohol fixation group,the positive rate of 2-min group (88.24％,15/17) was significantly higher than the 5-min group (53.85％,7/13) with statistical significance (x2 =4.535,P =0.033).Conclusion Compared with conventional fixation,alcohol fixation and liquid-based cytology can improve positive diagnostic rate of brushing during ERCP.The alcohol fixation is more simple in procedure and low in cost than liquid-based cytology.The interval time between sampling and fixation should be reduced to no more than 2 minutes.

Objective To evaluate curative effects of treatment of malignant biliary and gastric outlet-duodenal obstruction with endoscopically placed self-expandable metal stents.Methods A retrospective analysis was performed in 17 patients who underwent enteral stenting after placement of the biliary stent.The success rate of insertion,the effective palliation of biliary and duodenal obstruction,the rate of complication,recurrent stent obstruction and the median patency were observed.Results In 17 patients,biliary stenting were all performed for obstructive jaundice and then enteral stents were inserted.The levels of tatal billirubin [from (263.4 ± 62.5) μmol/L to (157.6 ± 25.1) μmol/L],direct billirubin [from (1233.2 ±66.5) μmol/L to (130.9 ± 27.7) μmol/L] and alkaline phosphatase [from (233.2 ± 66.5) IU/L to (130.9 ±27.7)IU/L] decreased significantly (P ＜0.01),and the gastric outlet score increased significandy [from (0.9 ± 1.1) points to (2.1 ±0.7) points] (P ＜0.01).No serious complication in all patients.Lifetime of patients ranged from 70 days to 332 days,and the median survival time was 192 day.Conclusion Combined biliary and enteral stenting is an effective method for palliation of malignant biliary and gastric outlet-duodenal obstruction.

Objective To study the diagnostic evaluation of spiral CT for acute pulmonary embolism(PE).Methods 24 patients with PE were scanned with SCT(Philips AVEL.)pre-and post-injection of 100 ml contrast medium(Ultravist or Omnipaque 300 mgI/ml)from elbow vein.Enhanced CT scans were started after injection of 15 and 30 seconds respecting.Results The indirect signs on plain CT image were:lung-making sparse in 12 cases;lesion of pulmonary infarction in 11 cases;pulmonary hypertension signs in 3 cases;pleural thickening in 4 cases;pleural effusions in 8 cases.The direct signs on enhanced CT images were:Intraluminal filling defect (mural filling defect in 32 vessels;partial filling defect in 30 vessels;total occlusion in 92 vessels and central filling defect that was railway-track sign in 15 vessels)and smaller caliber of pulmonary artery in 14 vessels.Conclusion Enhanced pulmonary SCT angiography is the safe,quick and effective imagilogic examination for acute PE.