The repair of ancient books as an important element in the protection of ancient books,has been attached great importance in China ever since.At the sairle time,the work is facing with heavy tasks,emergent time,and lack of professional personnel.Besides,due to being emphasized too much on the experience of traditional handiworks for all the time,the work is absence of modern research,theoretical conclusion,scientific standardized management,and fund supporting.All these problems need to be solved rationally.

Necroptosis, or programmed cell death, is a type of cell death with a controllable death signaling pathway and the morphological features similar to necrosis.It is mainly mediated by death receptors or pathogen pattern re-cognition receptors.Among them, tumor necrosis factor receptor 1 (TNFR1)-mediated necroptosis is the most well-studied one.Receptor-interacting protein kinase 1 (RIPK1) and receptor-interacting protein kinase 3 (RIPK3) are the 2 key kina-ses involved in the formation of complex I & II and necrosome in the process of necroptosis.Phosphoglycerate mutase 5 ( PGAM5) , a member of phosphoglycerate mutase gene family, lacks PGAM activity and possesses the phosphatase activi-ty.PGAM5 is anchored in the mitochondrial membrane and is also called mitochondrial phosphoglycerate mutase 5.It has been shown that PGAM5 involves in the formation of necrosome during necroptosis and it is able to accelerate the fission of mitochondria by dephosphorylation of dynamin-related protein 1 (DRP1), thus promoting cell necroptosis.

Objective: To analyze some influence factors during isokinetic eccentric test. Method: Twenty normal males were tested by Biodex isokinetic test and rehabilitation system. Their peak torque(PT) and peak torque to body weight (PT/BW) were analyzed under different conditions. Result: It was found that velocity and contraction mode influence the results significantly( P＜0.01). Conclusion: Many factors must be considered during isokinetic eccentric test and training.

Objective To investigate the effect of netropsin on migration and invasion ability of gastric cancer cells and its mechanisms.Methods To determine if netropsin inhibits migration and invasion of gastric cancer cells, Transwell migration and invasion assay was performed.Then Western blot was performed to detect expression of E-cadherin and vimentin in gastric cancer cells with or without presence in medium netropsin.Finally, immunofluorescence was performed to detect changes in the cellular localization of β-catenin to validate whether Wnt/β-catenin pathway was suppressed or not.Results Netropsin with the concentration of 25 μmol/L had minimal inhibition effect on cell proliferation and was able to suppress ability of migration and invasion by inhibiting EMT in gastric cancer cells(P<0.05).Meanwhile netropsin was able to down-regulate the expression of epithelial markers E-cadherin and up-regulate the expression of mesenchymal marker vimentin.Finally,immunofluorescence showed that netropsin was able to block translocation of β-catenin from cytoplasm to nuclear.Conclusions Netropsin can inhibit EMT thereby suppressing migration and invasion of gastric cancer cells.The mechanism is that netropsin can compete with HMGA2 for transcription factor binding site thereby suppressing the Wnt/β-catenin pathway.

Objective:Through the analysis of the 64 rows of 128 slice spiral CT two years usage data, establishes CVP analysis model.Methods: Using the rate of return on investment, the investment recovery period, cost recovery rate, the amount of profit, data analysis method, etc.Results: Comprehensive embodiment of equipment through various analysis methods of operation efficiency, to use department and management department to provide relevant data, and provide reference for the rational use of equipment.Conclusion: Through the large medical equipment benefit analysis, can make the hospital more clear understanding of medical equipment to hospitals with economic benefits, so as to improve the efficiency in the use of equipment, reduce the waste of money.

Objective To analyze the present research situation of stroke rehabilitation nursing in China, find out the defects and shortcomings existing in the development, and lay a good foundation for the development of stroke rehabilitation nursing. Methods The documentation published in Chinese core journals was searched in Chinese National Knowledge Infrastructure (CNKI), VIP database (VIP), Wanfang database by computer retrieval. The publishing year, journal name, scientific research funds, the method of literature research, research objects and research contents were analyzed by bibliometrics. Results A total of 253 articles were included with an increasing trend. These literature distributed in 34 kinds of Chinese core journals, fund rate was relatively good, but very few national fund, the experimental research was given priority to, and the research content was wide. Conclusions In order to better promote the rehabilitation of brain stroke patients, community rehabilitation care needs to be vigorously promoted, emphasis should be paid to the importance of patients family members, at the same time, great importance should also be attached to the combination between disciplines, in order to find better, more effective and easy methods to promote the rehabilitation nursing.

OBJECTIVE: To study the pharmacological activities and toxicity of the crude alkaloids of Toddalia asiatica and to provide pharmacological data for the further development of this herbal medicine. METHODS: We observed the anti-inflammatory effects of the crude alkaloids of Toddalia asiatica, using xylol and agra to induce the turgidness and sodium carboxymethyl cellulose (CMC-Na) to induce leucocyte strolling in the rats. The analgesic effects were observed by body-distortion methods. The effects of alkaloids of Toddalia asiatica on hepatic function were observed by testing the contents of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum and calculating the liver index. The LD 50 and 95% creditability were calculated with developed Karber Method. RESULTS: The administration of alkaloids of Toddalia asiatica had the function of inhibiting the auricle swelling caused by xylol and joint swelling caused by agar and leucocyte migration caused by CMC-Na, decreasing the body-distortion of the rats. After two weeks administration, the contents of ALT and AST showed that there was no obvious difference between administered group and control group. The LD50 of the crude alkaloids of Toddalia asiatica was 1.622 g/kg and the 95% creditability was 1.29-2.03 g/kg. CONCLUSION: Toddalia asiatica has anti-inflammatory and analgesic effects, and there is no injury to the liver after long-term administration in rats.

Objective To study the effect of salmon calcitonin and physical therapy on lumbar spinal ste- nosis.Methods Eighty cases of lumbar spinal stenosis were divided into treatment and control groups.Physical therapy alone was given to the control group,but salmon calcitonin was injected intramuscularly in addition to phys- ical therapy for the patients in the treatment group.Visual analogue scale,range of motion (ROM),pain-free walking distance,tendon reflexes and functional independence measures (FIM) were observed to assess the re- sults.Results The VAS,ROM and walking distance of the treatment group improved more than those of the con- trol group,but the groups' tendon reflexes and FIM were similar.Conclusions Salmon caleitonin can reduce the symptoms of lumbar spinal stenosis,and it has special effects in relieving pain.

OBJECTIVE: To investigate the bionomics of the olfactory ensheathing cells (OECs) of human olfactory mucosa. METHOD: To separate and cultivate the OECs of human and rat olfactory mucosa. To observe the cell growth, cell grouping and cell migration in vitro of the two types of OECs. RESULT: Successfully separated and cultivated the OECs of human and rat olfactory mucosa. OECs of the human and rat olfactory mucosa had the similar cell growth, cell grouping and cell migration ability in vitro. CONCLUSION OECs of the human and rat olfactory mucosa have the similar bionomics in vitro, as a result, OECs of the human olfactory mucosa could be a reliable source of cell transplant for nerve injury.
Animals ; Cell Culture Techniques ; Cell Movement ; Cells, Cultured ; Humans ; Olfactory Mucosa ; cytology ; Rats

Objective To study the efficacy of voice therapy combined with Jinsangsanjie pill in patients with vocal nodule .Methods A total of 146 patients with vocal nodes were randomly divided into three groups :45 cases in group A(single Jinsangsanjie pill therapy) ,47 cases in group B(single voice therapy) and 54 cases in group C(voice therapy combined with Jinsangsanjie pill therapy) ,30 healthy adults were as a normal control group .The treatment lasted 1 month .The results were evaluated by voice handicap index ,voice acoustic analysis and electronic laryngo‐scope which were collected before and after 1 month treatments .Results The VHI ,jitter ,shimmer and NNE of all patients were reduced while the MPT was increased after the treatment .The differences were significant (P<0 .05) .VHI 3 .64 ± 2 .12 ,jitter 0 .30% ± 0 .08% ,shimmer 1 .41% ± 0 .31% ,NNE -16 .83 ± 5 .84 dB of group C were significantly lower while MPT 18 .87 ± 3 .38 s and cure rate(93 .5% ) were significantly higher than those of in groupA(7.39±2.56,0.38% ±0.12% ,1.78% ±0.41% ,-13.38±4.42dB,16.38±3.11s,80.5% )andgroupB (23.6±12.5,0.48% ±0.18% ,1.98% ±0.42% ,-9.42±3.82dB,14.98±3.28s,52.4% )(P< 0.05).The VHI ,,jitter shimmer ,NNE of group A were significantly lower .MPT and cure rate were significantly higher than those of in group B .There were no statistical differences in each vocal indicators between group C and the normal control group (P>0 .05) .Conclusion The voice therapy combined with Jinsangsanjie pill in the treatment of vocal nodules is more efficiently ,and can improve patient’s pronunciation features .