Objective To investigate the reason and prevention of severe hyponatremia in hysteroscope treatment of the submucous myoma.Method Retrospective study was done on 106 cases of submucous myoma,3 cases with severe hyponatremia after hysteroscope treatment.Results The rate of hyponatremia was 2.8％(3/106),patients appeared nausea and vomiting 1 hour after hysteroscope treatment,blood sodium was lower than 110 mmol/L,volume of distention liquid was 8000-10000 ml,the volume of discharge was 6300-7500 ml.Three patients were treated immediately,1 patient was given sodium lactate Ringer injection and 0.9％ sodium chloride by intravenous drip,and furosemide by discontinuous injection 15 hours later,the hyponatremia was recovery; 2 patients were given 3％ sodium chloride and 15％ potassium chloride by intravenous drip,and furosemide by injection,12 hours later,the hyponatremia were recovery.Conclusion The prevention of severe hyponatremia complication after hysteroscope treatment of the submucous myoma not only need operate carefully but also need take active preventive measures.

Objective To study the effects of light damages of epidermal permeability barrier on cosmetics function evalu-ation.Methods 10volunteers were selected during the1st stage i.e.the3-month experiment of cosmetics function carried out using the traditional method.The skin moisture,transepidermal water loss and skin elasticity were measured,and the required sample size was estimated also.The2nd stage of the experiment of cosmetics function was carried out based on the data of the1st stage.The skin surface was cleaned with the detergent containing sodium lauryl sulfate with mass fraction of5%to induce light damages in skin surface before testing with cosmetics every day,those indexes mentioned above were measured also.Re -sults The1st stage of the experiment using the traditional evaluation method showed that the skin moisture,transepidermal wa-ter loss and skin elasticity revealed significant differences in the3rd month after testing with cosmetics compared with those be-fore testing with out cosmetics(P

The effects of Amrinone (AMR)on left ventricular function in 30 cases of congestive heart failure patients was assessed by M-mode and pulsed Doppler echocardiography. AMR improves the systolic and diastolic function of left ventricle during and after 10 min (AMR lmg ? kg-1, infusion ). The mean stroke volume increased 20% and 18% ; cardiac output increased 23% , 19% ; cardiac index increased 23% and 23% ; ejection fraction increased 32% , 30%; mVcf increased 50% and 48% ; filling velocity in early diastole increased 30% and 21 %; respectively. There were no significant differently between during and after AMR infusion, but there were signifcantly difference a-mong before, during and after AMR infusion. The heart rate changed slightly without statistic significance. The results suggest that AMR could improve the systolic and diastolic function of left ventricle in congestive heart failure patients.

The ultrastructural localization of acid phosphatase in the cells of mouse thymic cortex was described by using Robinson and Karnovsky method with cerium chloride as capture agent. The results indicated that the enzyme activity was localized mainly on the plasma membrane of epithelial reticular cells and thymocytes, especially where both of them were in contact with each other. Acid phosphatase activity was also found in the invaginations of plasma membrane, the vesicles near the plasma membrane, the saccules of the Golgi zone, the lysosomes, and a part of the vacuoles of epitheleial reticular cells. The lysosomes and the dense granules of cytoplasm, as well as on the some area of plasma membrane of the macrophages showed the enzyme activity. The enzyme activity was demonstrated in the cytoplasm and on the plasma membrane of degenerated thymocytes. The role of acid phosphatase in thymic cortex cells was discussed.

The thymic cortex is the important site for thymocyte differentiation, maturation and selection. Direct cell-cell interactions between thymocytes and distinct stromal cells are an important pattern to demonstrate the essential role in T-cell development. In order to approach the mechanism of their interactions, ultrastructural cytochemical localization of 5'-nucleotidase activity in the thymic cortex of BALB/c mice were investigated by means of Robinson and Karnovsky method using 5'-AMP or 5'-IMP as the substrate, cerium as the capture agent, and levamisol (an inhibitor of nonspecific alkaline phosphatase) was also used. The cytochemical distribution of the reaction products from both the 5'-AMPase and 5'-IMPase activity was essentially similar. The enzyme activity was localized on the extracellular side of the plasma membrane of the epithelial reticular cells and some thymocytes, especially on their contact faces with each other. 5'-nucleotidase activity was also found in the lysosomes of macrophages and in the vacuoles, and vesicles near the plasma membrane and some lysosomes of epithelial reticular cells. The biological significance of the 5'-nucleotidase in thymic cortex was discussed.

A murine MTEC_1 thymus epithelial cell line established by us has been characterized. The cells were polyhydral and closely packed each other as epithelial like cells. Using anti-keratin antibody, the keratin were shown in cytoplasm of all cells. Under electronmicroscope, bunches of tonofilaments were clearly shown in the cytoplasm, and desmosomes were seen between neighbouring cells. Using anti-mouse epithelial cell monoclonal antibodies for immunohistochemical study, nearly all of the MTEC_1 cells were MTS33 positive. It suggests that MTEC, cells were derived from the epithelial cells located in medulla. The majority of the MTEC_1 cells have normal mouse diploid chomosome number of 40. These results provide evidence that MTEC, cell line is normal murine thymus epithelial cells.

Fresh cryostat sections from the thymus of BALB/c adult mice were processed with semipermeable membrane technique for demonstration of non-specific esterase (NE). The various cell types and the pattern of their distribution may be showed, because this technique may preserve the total enzyme activity of cells. The NE activity of epithelial reticular cells (ERC), thymic nurse cells (TNC), and interdigitating cells (IDC) are lower, but macrophages (M?) show a high activity. Cortical ERCs appear as a sponge-like network. Medullary ERCs may be divided into two cell types, i. e. low and high activity cells. The distribution of the latter shows foci-like pattern. M? present in both the cortex and medulla, in the cortico-medullary border they are prominent and may form rosettes-like structure with thymocytes. The thymus was also studied with immunohistochemical method using the monoclonal antibody of rat-anti-mouse thymic stromal ceils (MTS-6). This observation enable studies on the type of thymic stromal cells formed thymic microenvironment under LM.

We investigated the effects of IFN-γ on Chlamydia psittaci (Cps) infection.HeLa cells were treated with different concentrations of recombinant human IFN-γ (5 ng/mL,25 ng/mL,50 ng/mL) after infecting with C.psittaci 6BC,then the number and morphology of C.psittaci inclusion bodies were examined after 48 hours.C57BL/6J mice were intranasally infected with 2 × 106 IFUs C.psittaci 6BC,and intraperitoneally administrated with 10 μg recombinant murine interferon-γ 24 hours prior or post infection,then body weight,activity and survival rate were recorded.The histopathology of mice livers and lungs was analyzed by HE staining on day 5 or day10 post infection.And the chlamydial inclusion bodies were titrated in the lung homogenates of mice sacrificed on day 5 after infection.The inclusion body numbers of recombinant human IFN-γ treated groups (by 5ng/mL,25ng/mL,50ng/mL) were significantly less than that in the control group (23.8±5.1)× 106,(10± 3.58) × 106,(8.0±2.22) × 106,(43.3±11.05)× 106,respectively).And the morphology of inclusion bodies in IFN-γ treated HeLa cells was irregular and much smaller.We also found that IFN-γ could significantly improve the survival rate,reduce acute clinical manifestations and pathological injurery of lung and liver in C.psittaci respiratory tract infected mice model.So we summarized that IFN-γ can mediate strong immunological protection during acute C.psittaci early infection.

Objective To explore the relationship between heart rate variability (HRV) and deceleration capacity (DC) in children with idiopathic ventricular premature contraction of different origins. Methods The clinical data from 155 children with idiopathic ventricular premature contraction were retrospectively analyzed. According to the age, the children were divided into young children group (

Objective To investigate the application value of single detection and combined detection of 4 kinds of inflammatory indicators of procalcitonin (PCT ) ,high sensitivity C-reactive protein(hs-CRP) ,interleu-kin-6(IL-6) and white blood cell(WBC) in diagnosing type 2 diabetes mellitus(T2DM) bloodstream by analy-zing the levels of peripheral blood PCT ,hs-CRP ,IL-6 and WBC in the T2DM bloodstream infection group and T2DM non-bloodstream infection group .Methods The clinical data in 85 patients with T2DM bloodstream in-fection (T2DM bloodstream infection group ) and contemporaneous 80 cases of T2DM non-bloodstream infec-tion(T2DM non-bloodstream infection group) in this hospital from January 2013 to July 2016 were retrospec-tively analyzed .The levels of various inflammatory indicators in peripheral blood were analyzed .The receiver operating characteristic(ROC) curve of various inflammatory indicators was drawn ,the area under the curve (AUC) and the best cut-off value were calculated .The detection schemes included 24 kinds of schemes such as the single indicator ,2-indicator ,3-indicator and 4-indicator .Results The levels of PCT ,hs-CRP ,IL-6 and WBC in the T2DM bloodstream infection group were significantly higher than those in the T 2DM non-blood-stream infection group ,the difference was statistically significant (P<0 .05) .AUC of PCT ,hs-CRP ,IL-6 and WBC were 0 .909 ,0 .818 ,0 .838 and 0 .760 respectively ,with best cut-off values of 0 .493 ng/mL ,11 .19 ng/mL ,40 .95 pg/mL and 11 .87 × 109/L respectively .The Youden index of PCT was highest (0 .65) and the ac-curacy of IL-6 was highest (83 .33％ ) in the single indicator detection scheme .The Youden index and accuracy of the scheme of PCT/hs-CRP and PCT+hs-CRP+IL-6 were highest in the combined detection scheme .Con-clusion PCT detection has the prominent value in the assisted diagnosis of T 2DM bloodstream infection .Inthe combined detection scheme ,PCT/hs-CRP and PCT+hs-CRP+IL-6 have the highest value in the assisted diagnosis in T2DM bloodstream infection .