1.The Effect of CGRP on Free Calcium Concentration in Brain Cells of Hypoxic-ischemic Neonatal SD Rats
Lanfang TANG ; Yiu WANG ; Jianxin TAN
Journal of Chinese Physician 2000;0(12):-
Objective To explore the effect of calcium gene-related peptide (CGRP) on free calcium concentration in the brain cells of hypoxic-ischemic neonatal SD rats. Methods Animal model of hypoxic-ischemic cerebral injury was set up using SD rats of 7 days old. Then the rats were randomly divided into treatment group given 3?g/kg/d CGRP intraperitoneally for 3 days immediately after the model was made, and salt solution group given 0.9% NaCl solution intraperitoneally for 3 days. Normal control group received sham operation. All the rats were decapitated after 3 days and the concentration of free calcium in brain cells was measured with calcium fluorescence indicator in Fura-2Am. Results The free calcium concentration in brain cells in salt solution group was significantly higher than that in the other two groups (P
2.Application of holmium: YAG laser treatment via bronchoscopy in the management of congenital vallecular cyst in infant.
Lei WU ; Yuanjian SHENG ; Lanfang TANG
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2015;29(16):1489-1491
Bronchoscopy
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Cysts
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congenital
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surgery
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Humans
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Infant
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Laryngeal Diseases
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congenital
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surgery
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Lasers, Solid-State
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Pharyngeal Diseases
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congenital
;
surgery
3.Post-infectious bronchiolitis obliterans in children: clinical features and bronchoalveolar lavage therapy
Yongxing ZHONG ; Meiping LU ; Zhimin CHEN ; Xinger BAO ; Lianxiang CHEN ; Lanfang TANG ; Guohong ZHU
Chinese Journal of Emergency Medicine 2010;19(11):1188-1192
Objective To investigate the clinical features and bronchoalveolar lavage (BAL)therapy of postinfectious bronchiolitis obliterans (BO) in children. Method Ten children, who had post-infectious BO from February 2009 to February 2010, received BAL therapy, and were retrospectively analyzed. The data included pathology,chnical feature,chest HRCT scan, BALF cellular, levels of blood T cell subtypes and outcome of BAL therapy. Results Adenoviruses or mycoplasma pneumoniae were the most common etiologic agents (4/10, respectively). All patients presented persistent or recurrent dyspneic respirations and wheezing since the initial lung infection. The findings of HRCT included mosaic pattern of perfusion (6/10), accompanied by gas retention,bronchiectasis, atelectasis and bronchial wall thickening. The percentage of neutrophils in BALF was significantly increased in all cases (10/10). There were predominance of CD8+ T cell subtype (9/10) and lower ratio of CD4 +/CD8+ ( 10/10)in blood. Reduced symptoms and shortened hospital stay of BO in 9 of all 10 cases were observed after BAL therapy. Conclusions Severe adenovirus or mycoplasma pneunoniae bronchiolitis and/or pneumonia has higher risk for developing BO in children. Increased percentage of neutrophils in BALF and predominance of CD8 +T cell subtype may play an important role in the mechanism of BO. BAL therepy can reduce the respiratory symptoms of BO in children.
4.The Effects of Keratinocyte Growth Factor and Its Receptors Antisense Oligonucleotide on Prolifera- tion of HaCat Cells
Erxun KANG ; Jiarun ZHENG ; Xinyu LI ; Jiwei GAO ; Lanfang XU ; Meiyu TANG
Chinese Journal of Dermatology 2003;0(10):-
Objective To investigate the effects of keratinocyte growth factor (KGF) and KGF receptor (KGFR) antisense oligonucleotide (ASODN) on cell cycle and apoptosis of HaCat cells. Methods HaCaT cell, an immortalized keratinocyte cell strain, was cultured in vitro. Flow cytometry was used to measure the cell cycle and apoptosis mediated by KGF and ASODN. Results The rates of S phase and apoptosis in the group treated with KGF increased significantly than those in the control group (both P
5.Progress of extracellular vesicles in the pulmonary inflammatory diseases
Junyan JI ; Yuqing CAI ; Lanfang TANG
International Journal of Pediatrics 2020;47(3):155-159
Extracellular vesicles are a kind of membrane structures deriving from cells, which exist widely in urine, blood, saliva, milk, cerebrospinal fluid, amniotic fluid and other body fluid.Extracellular vesicles contain nucleic acid, enzymes, proteins and other bioactive molecules, which can convey the biological information to target cells through a variety of modes and participate in the pathophysiological processes of target cells.In recent years, it is considered that extracellular vesicles are associated with the pathogenesis of inflammatory disease of pulmonary such as asthma, chronic obstructive pulmonary disease and acute lung injury/acute respiratory distress syndrome, and have potential therapeutic value through a large body of research.
6. Diagnosis and intervention of congenital cystic adenomatoid malformation
International Journal of Pediatrics 2019;46(11):790-793
Congenital cystic adenomatoid malformation(CCAM) is a rare cystic lung disease with an incidence rate ranging from 1/35 000 to 1/25 000 births.The mechanism is still unclear and is regarded a nonhereditary anomaly caused by arrest of lung.In some countries, over 99% of CCAMs were diagnosed by fetal sonography at gestational age of 18~20 weeks.Chest X-ray and CT are common methods to detect the CCAMs after birth.Newborns with CCAM may present with respiratory distress, but most patients are asymptomatic in life.Although many methods for diagnosis and treatment were suggested, no unified guideline is provided yet.Herein, we reviewed the advances in the mechanism, diagnosis and treatment of CCAM to highlight this rare event.
7. Clinical analysis of seven cases of pulmonary malignant tumors in children
Dan XU ; Zhimin CHEN ; Weizhong GU ; Yingshuo WANG ; Meixia HUANG ; Lanfang TANG ; Yuanyuan ZHANG ; Yuan JIANG
Chinese Journal of Pediatrics 2017;55(4):298-303
Objective:
To understand the clinical manifestation, imaging characteristics and outcomes of pulmonary malignant tumors in children.
Method:
We retrospectively collected information about seven cases of pulmonary malignant tumors in children in our hospital from Jan 2010 to Dec 2016. The information included clinical manifestation, imaging characteristics, pathologic results, and treatment.
Result:
(1) All the seven patients firstly visited pediatric internal medicine departments. Symptoms included cough (
8. Clinical features of pulmonary thromboembolism of eight children
Feizhou ZHANG ; Jiexin YUAN ; Xiaofen TAO ; Zhimin CHEN ; Lanfang TANG
Chinese Journal of Pediatrics 2020;58(1):25-29
Objective:
To improve the diagnosis and therapy of childhood pulmonary thromboembolism (PTE) by analyzing the clinical features of this rare condition.
Methods:
A total of 8 pediatric patients (4 males, 4 females) with PTE diagnosed in the Children′s Hospital of Zhejiang University School of Medicine from March, 2014 to March, 2019 were enrolled. The clinical manifestation, laboratory results, imaging findings, diagnosis and treatment were summarized.
Results:
Among these 8 cases, aged from 9 hours to 14 years and 10 months. Fever was found in 4 cases, cough aggravation in 4, short of breath in 3, chest pain in 2, abdominal and back pain in one, hemoptysis in 2, cyanosis in 1, and edema of lower extremities in 2. Physical examination found decreased breath sound in 2 cases, phlegm rale in 3, and pleural friction rub in one. Pleural effusion was found in 5 cases by ultrasound. Plasma D-dimer increased in 6 cases (0.66-9.96 mg/L) and hypersensitive C-reactive protein elevated in 5 cases (10.78-78.00 mg/L). Chest enhanced CT showed pulmonary artery or venous filling defects, including pulmonary artery embolism in 7 cases and pulmonary vein embolism in one. The primary disease of these patients included
9. Selection and application opportunity of antibiotics in children with respiratory tract infections
Xuefeng XU ; Yuanjian SHENG ; Lanfang TANG ; Zhimin CHEN
Chinese Journal of Applied Clinical Pediatrics 2019;34(22):1751-1754
Respiratory tract infection is the most common disease in children, and it is also the most common disease of irrational use of antibiotics.The rational application of antibiotics is to reduce the rate of bacterial resistance and its adverse events.The emergence of drug-resistant strains is closely related to the wide application of antibiotics.In order to reduce the irrational use of antibiotics in children′s respiratory tract infection, pediatricians need to know the timing of application of antibiotics.The respiratory tract infection can be divided into upper respiratory tract infection and lower respiratory tract infection, including common cold, pharyngitis, sinusitis, otitis media, bronchitis and bronchitis, and pneumonia.This article reviews the application timing of antibiotics in the common respiratory tract infection of these children.
10. Multisystemic smooth muscle dysfunction syndrome in children: a case report and literature review
Yunlian ZHOU ; Yuanyuan ZHANG ; Beilei CHENG ; Dan XU ; Lanfang TANG ; Zhimin CHEN
Chinese Journal of Pediatrics 2017;55(8):619-623
Objective:
To analyze the clinical characteristics and diagnosis of multisystemic smooth muscle dysfunction syndrome(MSMDS).
Method:
Clinical data of a case diagnosed as MSMDS and hospitalized in our hospital in July 2016 was retrospectively analyzed. Literature search was performed at databases of PubMed, Wanfang, China National Knowledge Infrastructure and VIP with the key words "multisystemic smooth muscle dysfunction syndrome" "ACTA2" . The literature retrieval was confined from January 1980 to November 2016.The characteristics of MSMDS were summarized through review of literature.
Result:
A girl aged 1.6 years had recurrent cough and wheeze for more than 1 year, complicated with congenital fixed dilated pupils, patent ductus arteriosus, pulmonary hypertension, chronic lung disease, and cerebrovascular abnormalities. We had done gene analysis for the patient and found ACTA2 c. 536C>T(p.R179H) heterozygous mutations, but her parents were normal. Totally 11 reports were retrieved from foreign language literature and no report from Chinese literature could be found; the retrieved articles reported a total of 25 cases of multiple system smooth muscle dysfunction syndrome. The minimum age was 11 months, 17 cases were female, 8 were male. The clinical common characteristic is congenital fixed dilated pupils, patent ductus arteriosus, cerebrovascular disease, pulmonary hypertension, chronic lung disease, and so on.
Conclusion
Genetic testing for ACTA2 gene mutations should be considered in infants presenting with congenital fixed dilated pupils and patent ductus arteriosus.