Objective To assess the effect of peroxisome proliferator-activated receptor γ (PPARγ) agonist on prostate epithelial cells in vitro.Methods The expression of peroxisome proliferator-activated receptor γ(PPARγ) was studied by immunocytochemistry and immunofluorescence study.The RWPE-1 human prostate epithelial cell line was treated with PPARγ agonist rosiglitazone 100 μmol/L for 48 h.Analysis of apoptosis was performed by Caspase 3/7 activity assay.Mitochondria depolarization was measured by using the potential-sensitive color,JC-1.The expression of apoptosis-related proteins-Bax was investigated by immunohistochemistry.Results PPARγ mainly located in nucleus and perinucleus.RWPE-1 cell line treated with PPARγ agonist rosiglitazone showed higher Caspase 3/7 activity (10636±1032 RLU) than in control (5936±620 RLU),P＜0.01 and significantly upregulated Bax level (8250±694 vs.6017±563)than in control group,P＜0.01.In addition,mitochondrial membrane potential was depolarized in rosiglitazone treated cells.Conclusions PPARmay play important roles in the pathophysiology of BPH.The mechanism might be that PPARγ regulates cell apoptosis.It is suggested that the mitochondrial and Bax pathway might be involved in signaling PPARγ induced cell apoptosis.

This study aimed at investigating the dose-effect relationship of SFI between the blood viscosity and the early-and mid-stage cardiogenic shock and the mediatory effect on rats.The end or root of left anterior descending coronary arteries (LADCA) was ligatured to establish the rat model of the early-and mid-stage cardiogenic shock.The blood viscosity indexes included low shear rate (LSR,10/s),middle shear rate (MSR,60/s),high shear rate (HSR,150/s) of the whole blood viscosity and plasma viscosity (PV),being observed 60 mins after the venous administration of 0.10,0.33,1.00,3.30,10.00 and 20.00 mL·kg-1 SFI (low dosage range:0.1-1.0 mL·kg-1,middle dosage range:1.0-10 mL·kg-1,high dosage range:10-20 mL·kg-1) with a blood rheometer.Dose-response curves were fitted by GraphPad Prism 6.0 software,the dose-response relationship of SFI between the blood viscosity and the early-and mid-stage cardiogenic shock in rats was evaluated to calculate the dose threshold parameters of the indexes.It was found that the blood viscosity indexes were improved with the dosage of 10 mL·kg-1 SFI in rats with the early-stage cardiogenic shock,while the dose-response curves of LSR,MSR and HSR at the early stage all presented favorable s shapes.Most of the effective dose range [D]2o-[D]80 and the threshold dose [D]20 were between 3.3 and 6.3 mL· kg-1.The four indexes of blood viscosity were improved with the administration of 10 and 20 mL·kg-1 SFI in mid-stage model rats with favorable s shapes in the dose-response curves.Most of the effective dose range and the threshold dose were in the range of 3.3 to 10.0 mL·kg-1.In conclusion,most of the dose-response curves of blood viscosity indexes in early-and mid-stage cardiogenic shock rats presented favorable s shapes with the threshold dose between 3.3 and 10.0 mL·kg-1,indicating an effective middle dosage range,which was converted into clinical dosage about 37.1 to 112 mL each day.The research provided an experimental basis for clinical medication.

This paper was aimed to study the effects of Huang-Lian Jie-Du decoction (HJD) on contents of oxidized low density lipoprotein (Ox-LDL),monocyte chemoattractant protein (MCP-1),vascular cell adhesion molecule (VCAM-1),malondialdehyde (MDA),and superoxide dismutase (SOD) of atherosclerosis (AS) rats' liver homogenate.Male SD rats were randomly divided into six groups,which were the normal control group,model group,positive control group (lovastatin),and the low-,middle-,high-dose HJD groups,with ten rats in each group.Except for the normal control group fed with normal diet,others were fed with high-fat diet,and regularly injected the vitamin D3.All groups were gavaged once daily from the 3rd week for 8 weeks until they were sacrificed.The results showed that compared with the normal control group,contents of MCP-1,VCAM-1 and Ox-LDL in the liver homogenate of the model group had significantly increased (p ＜ 0.05),after drug administration,all indexes mentioned above in the positive control group,the low-and middle-dose HJD group were decreased significantly (p ＜ 0.05);contents of MCP-1 and VCAM-1 of the high-dose HJD group decreased obviously (p ＜ 0.01);MDA content of liver homogenate increased significantly,after drug administration,contents of the positive control group and the low-dose HJD group decreased significantly (p ＜ 0.05);SOD content decreased significantly,after drug administration,contents of both the positive control group and the high-dose group increased significantly (p ＜ 0.05).It was concluded that HJD may play a role in AS intervention.Its mechanism may be related to its anti-inflammatory and antioxidant reaction.