Objective To observe the effect of pre-intervention with electroacupuncture on oxidative stress (OS) and inflammatory reaction after hepatectomy under portal occlusion.Method Thirty patients going to receive hepatectomy under Pringle's portal occlusion were randomized into an electroacupuncture group and a sham electroacupuncture group, 15 cases each. Three days prior to the surgery, the patients started to receive corresponding acupuncture or sham electroacupuncture treatment by selecting bilateral Riyue (GB24), Qichong (ST30) and Yanglingquan (GB34), once a day. The anesthesia method was general (inhalational and intravenous) anesthesia. Central venous blood was drawn before the surgery, and respectively 6 h, 24 h, 48 h and 72 h after the surgery to examine the OS-relatedparameters, superoxide dismutase (SOD) and malondialdehyde (MDA), as well as the inflammatory mediators, including tumor necrosis factor-α(TNF-α), interleukin (IL)-1, IL-6, and IL-10.Result Compared to the sham electroacupuncture group, the SOD activity significantly increased (241±11.4 U/mL,180±12.6 U/mL,P<0.05) and the MDA level significantly dropped in the electroacupuncture group at 6 h and 24 h after the surgery(11.0±0.8 mmol/L,10.0±0.9 mmol/L,P<0.05); there were no significant between-group differences in the rest indexes (P>0.05).Conclusion Pre-intervention with electroacupuncture can up-regulate the activity of SOD and down-regulate the level of MDA in patients after hepatic portal occlusion, and thus partially reduce OS, but it doesn't shownoticeable effecton inflammatory response.

Objective To analyze the clinical value and safety of capsule endoscopy in the digestive tract diseases.Methods 55 cases of inpatients or outpatients were given the capsule endoscopy examination.The positive detection rate and safety were observed.Results The positive detection rate was 87.3％,the diagnostic rate was 50.9％.Capsule retention in the body happened in 2 cases (1 case of small intestine space-occupying lesions,decided to take the operation,another 1 case got Crohn's disease,intestinal incomplete obstruction),the occurrence rate was 1.5％.Conclusion The capsule endoscopy make up some shortcomings of the traditional gastrointestinal endoscope,but also show some drawbacks,so improving performance of instrument of the capsule endoscopy,expanding the scope of application of capsule endoscopy will be the development direction of the capsule endoscopy in the future.

A method for the detection of residual amount of pentachloro nitro benzene (PCNB ) and benzene hexachloride isomers, in Ginseng was developed. The pesticides in Ginseng was extracted with acetone-n-hexane (1∶ 2 ), purified with H2SO4, separated on ov-1701 flexible glass capillary column and determined by electron capture detector (ECD ).

Child ; Female ; Humans ; Paraquat ; poisoning ; Poisoning ; therapy ; Young Adult

0.05).The blood loss was significantly higher for women who had the abdominal approach(t=3.214,P

Objective To observe the distributive characteristics of glial cell line-derived neurotrophic factor(GDNF)in brain tissue during cerebral ischemia-reperfusion in rats,and its role in ischemic brain damage.Methods The model of focal cerebral ischemia was made by occluding middle cerebral artery(MCA) for 2 h and reperfusion for 0.5～48 h, HE Staining was used to investigate the histological features of ischemic cerebral damage,the immunohistochemical method was used to observe the distributive characteristics of GDNF in brain tissue in rats. Results The focal ischemic areas (including preoptic area, striatum and cortex) presented at 0.5 h of reperfusion and peaked at 24 h. The neurons presented irreversible degeneration at 6 h of reperfusion. At 24 h, the ischemic area in the preoptic area developed into infarct form. At 0.5 h of reperfusion, neurons in ischemic cortex showed GDNF weak positive, neurons in peri-ischemic regions showed GDNF moderate positive. During reperfusion 3～48 h, neurons in ischemic regions showed GDNF negative. Up to 48 h of reperfusion, the active microglias or macroglias in periinfart area strongly expressed GDNF.The number of GDNF positive cells in all groups decreased (P

ObjectiveTo investigate the effect of rehabilitation time on the motor function and activities of daily living (ADL) of patients with intracerebral hemorrhage.Methods76 patients were randomly divided into the early rehabilitation group (40 cases) and late rehabilitation group (36 cases). The patients of two groups were treated with Bobaths method. The starting times of rehabilitation were 48 h～7 d (early rehabilitation group) and 3～4 weeks (late rehabilitation group) after onset. The therapeutic effect was assessed with Fugl-Meyer Motor Assessment Scale (FMA), modified Barthel index (MBI) and neurological deficit evaluation (ND).ResultsThe scores of the FMA, MBI and ND were not different between two groups before treatment. After one month treatment, the scores of patients in two groups improved significantly, but the early rehabilitation group had a greater improvement compared with the late rehabilitation group. Conclusion Early rehabilitation and late rehabilitation can also facilitate the motor function and ADL of patients with intracerebral hemorrhage recovery, and the therapeutic effect of early rehabilitation is better than late rehabilitation significantly.

Animals ; Bone Marrow ; metabolism ; Cell Division ; physiology ; Hematologic Diseases ; therapy ; Hematopoiesis ; physiology ; Hematopoietic Stem Cells ; cytology ; Humans ; Renin-Angiotensin System ; physiology

Objective To observe the dynamic changes of vascular endothelial growth factor (VEGF) levels in the patients with acute intracerebral hemorrhage during different phases and study the relationships between the VEGF levels and the volume of hemorrhage or stroke severity. Methods Fifty-six patients with acute intracerebral hemorrhage were divided into three groups by the volume of hemorrhage:group A(＜ 20 ml,23 caees),group B(20-30 ml, 18 cases),group C(＞ 30 ml, 15 cases) and light group (1-15 scores,22 cases),midrange group (16-30 scores,20 cases),severe group (31-45 scores, 14 cases)by China stroke scale (CSS) score in 24 hours of hospitalization. The serum VEGF level was determined at 24 h,48 h,72 h,7 d and 14 d after the onset of disease by enzyme linked immunosorbent assay(ELISA).Results The serum levels of VEGF during different phases (within 24 h,48 h,72 h,7 d,14 d) had statistical differences between group A and group C (P ＜0.01). Within 24 h,48 h,72 h,7 d,there was statistical difference between group B and group C (P ＜0.01).Within 48 h,72 h,7 d,14 d,there was statistical significance between group A and group B (P＜ 0.05). The serum levels of VEGF during different phases(24 h,48 h,72 h,7 d, 14 d) were higher in severe group than those in light group(P＜ 0.01). Within24 h,48 h,72 h,7 d,there was statistical difference between severe group and midrange group (P＜ 0.01).Within 48 h, 72 h, 7 d, 14 d, there was significant difference between midrange group and light group (P ＜0.05). The volume of hemorrhage had positive correlation with the CSS scores of neurologic impairment on admission (r = 0.916, P ＜ 0.05). Conclusion The serum VEGF levels might be related to the volume of hemorrhage and the severity of the disease.

Protein ubiquitination is one of the most important and widely exist protein post-translational modifications in eukaryotic cells, which takes the ubiquitin and ubiquitin chains as signal molecules to covalently modify other protein substrates. It plays an important roles in the control of almost all of the life processes, including gene transcription and translation, signal transduction and cell-cycle progression, besides classical 26S protesome degradation pathway. Varied modification sites in the same substrates as well as different types of ubiquitin linkages in the same modification sites contain different structural information, which conduct different signal or even determine the fate of the protein substrates in the cell. Any abnormalities in ubiquitin chain formation or its modification process may cause severe problem in maintaining the balance of intracellular environment and finally result in serious health problem of human being. In this review, we discussed the discovery, genetic characteristics and the crystal structure of the ubiquitin. We also emphasized the recent progresses of the assembly processes, structure and their biological function of ubiquitin chains. The relationship between the disregulation and related human diseases has also been discussed. These progress will shed light on the complexity of proteome, which may also provide tools in the new drug research and development processes.
Humans ; Proteome ; Ubiquitin ; chemistry ; Ubiquitination